High-throughput production of human proteins for crystallization: the SGC experience. (Q34124142)

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5 February 2020

title

High-throughput production of human proteins for crystallization: the SGC experience (English)

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5 February 2020

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5 February 2020

Christopher D. Cooper

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5 February 2020

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5 February 2020

Pavel Savitsky

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5 February 2020

Brian D Marsden

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5 February 2020

Pravin Mahajan

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5 February 2020

Nicola A Burgess-Brown

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5 February 2020

publication date

10 June 2010

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Journal of Structural Biology

5 February 2020

published in

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5 February 2020

volume

172

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5 February 2020

issue

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5 February 2020

page(s)

3-13

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The completion of the Mammalian Gene Collection (MGC).

5 February 2020

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Large-scale structural biology of the human proteome

5 July 2018

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Structural coupling of SH2-kinase domains links Fes and Abl substrate recognition and kinase activation

5 July 2018

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Baculovirus expression vector system: an emerging host for high-throughput eukaryotic protein expression

5 July 2018

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Protein production and purification

5 July 2018

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A systematic interaction map of validated kinase inhibitors with Ser/Thr kinases

5 July 2018

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5 July 2018

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Mutations of Bacteria from Virus Sensitivity to Virus Resistance

5 July 2018

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5 July 2018

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Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination

5 July 2018

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Combinatorial Domain Hunting: An effective approach for the identification of soluble protein domains adaptable to high-throughput applications

5 July 2018

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First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value.

5 July 2018

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In vivo and in vitro protein solubility assays using split GFP.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2938586

An efficient and generic strategy for producing soluble human proteins and domains in E. coli by screening construct libraries

5 July 2018

1 reference

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Combinatorial library approaches for improving soluble protein expression in Escherichia coli

5 July 2018

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Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses

5 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2938586

FoldIndex: a simple tool to predict whether a given protein sequence is intrinsically unfolded

5 July 2018

1 reference

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Hsp90 and Cdc37 -- a chaperone cancer conspiracy.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2938586

CD-Search: protein domain annotations on the fly

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2938586

GlobPlot: Exploring protein sequences for globularity and disorder

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2938586

Medium-scale structural genomics: strategies for protein expression and crystallization

5 July 2018

1 reference

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A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2938586

The PSIPRED protein structure prediction server

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2938586

Protein secondary structure prediction based on position-specific scoring matrices

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2938586

SMART, a simple modular architecture research tool: identification of signaling domains

5 July 2018

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The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression

5 July 2018

1 reference

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Ligation-independent cloning of PCR products (LIC-PCR)

5 July 2018

1 reference

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Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates.

5 July 2018

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High throughput production of recombinant human proteins for crystallography

28 October 2018

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Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.

28 October 2018

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The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins.

28 October 2018

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Recombinant protein expression and solubility screening in Escherichia coli: a comparative study.

28 October 2018

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Scalable high-throughput micro-expression device for recombinant proteins.

28 October 2018

1 reference

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Improvement of the GenTHREADER method for genomic fold recognition

28 October 2018

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Strategies for structural proteomics of prokaryotes: Quantifying the advantages of studying orthologous proteins and of using both NMR and X-ray crystallography approaches

28 October 2018

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High throughput methods for gene cloning and expression.

28 October 2018

1 reference

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XtalPred: a web server for prediction of protein crystallizability

28 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2938586

Z(basic)--a novel purification tag for efficient protein recovery

28 October 2018

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12 December 2020

A pilot project to generate affinity reagents to human proteins

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12 December 2020

Creating a structural genomics consortium

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12 December 2020

Protein structures hint at the shape of things to come

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12 December 2020

10.1016/J.JSB.2010.06.008

Identifiers

DOI

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5 February 2020

1 reference

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5 February 2020

PubMed publication ID

20541610

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