Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR. (Q33706250)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:9464406%20AND%20SRC:MED&resulttype=core&format=json

9 November 2019

title

Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR (English)

1 reference

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9 November 2019

D P Chandler

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9 November 2019

C A Wagnon

series ordinal

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9 November 2019

H Bolton

series ordinal

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9 November 2019

language of work or name

English

0 references

publication date

1 February 1998

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Applied and Environmental Microbiology

9 November 2019

published in

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9 November 2019

volume

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9 November 2019

issue

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9 November 2019

page(s)

669-677

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Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein

9 November 2019

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1 reference

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Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies

14 July 2018

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Estimation of the abundance of an uncultured soil bacterial strain by a competitive quantitative PCR method

14 July 2018

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Manganese peroxidase mRNA and enzyme activity levels during bioremediation of polycyclic aromatic hydrocarbon-contaminated soil with Phanerochaete chrysosporium

14 July 2018

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Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples

14 July 2018

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Epstein-Barr virus and Hodgkin's disease: transcriptional analysis of virus latency in the malignant cells

14 July 2018

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Detection of poliovirus, hepatitis A virus, and rotavirus from sewage and ocean water by triplex reverse transcriptase PCR.

14 July 2018

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Quantitation of fungal mRNAs in complex substrates by reverse transcription PCR and its application to Phanerochaete chrysosporium-colonized soil

14 July 2018

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Detection and counting of Nitrobacter populations in soil by PCR.

14 July 2018

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Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast

14 July 2018

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Thermococcus peptonophilus sp. nov., a fast-growing, extremely thermophilic archaebacterium isolated from deep-sea hydrothermal vents

14 July 2018

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Simple method of concentrating enteroviruses and hepatitis A virus from sewage and ocean water for rapid detection by reverse transcriptase-polymerase chain reaction.

14 July 2018

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Detection of Norwalk virus and hepatitis A virus in shellfish tissues with the PCR

14 July 2018

1 reference

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Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids

14 July 2018

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An improved double stranded DNA sequencing method using gene 32 protein.

14 July 2018

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Reverse transcription of mRNA by Thermus aquaticus DNA polymerase

14 July 2018

1 reference

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Improved yields of long PCR products using gene 32 protein

14 July 2018

1 reference

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PCR based diagnosis in the presence of 8% (v/v) blood

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=106100

Reverse transcriptase inhibits Taq polymerase activity

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=106100

Reconstruction of bacteriophage T4 DNA replication apparatus from purified components: rolling circle replication following de novo chain initiation on a single-stranded circular DNA template

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=106100

Population Ecology of Nitrifying Bacteria

14 July 2018

1 reference

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In Vivo Interactions of Genes and Proteins in DNA Replication and Recombination of Phage T4

14 July 2018

1 reference

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Probing DNA Replication Mechanisms with the T4 Bacteriophage In Vitro System

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=106100

Recent excitement in the DNA replication problem

14 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=106100

Electron Microscope Measurements of Rous Sarcoma Virus RNA

14 July 2018

1 reference

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Isolation of nucleic acid-binding protein: stimulation of reverse transcriptase-catalysed DNA synthesis

14 July 2018

1 reference

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Estimating biodegradative gene numbers at a JP-5 contaminated site using PCR.

14 July 2018

1 reference

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Measurement by the polymerase chain reaction of the Epstein-Barr virus load in infectious mononucleosis and AIDS-related non-Hodgkin's lymphomas

30 October 2018

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Continuous RT-PCR using AMV-RT and Taq DNA polymerase: characterization and comparison to uncoupled procedures.

30 October 2018

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A coupled one-step reverse transcription PCR procedure for generation of full-length open reading frames.

30 October 2018

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31 Reverse transcription of mRNA by Thermus aquaticus DNA polymerase followed by polymerase chain reaction amplification

30 October 2018

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12 December 2020

32 Absolute levels of mRNA by polymerase chain reaction-aided transcript titration assay

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12 December 2020

33 Polymerase chain reaction-based mRNA quantification using an internal standard: Analysis of oncogene expression

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12 December 2020

Amplification, detection, and automated sequencing of gibbon interleukin-2 mRNA by Thermus aquaticus DNA polymerase reverse transcription and polymerase chain reaction

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12 December 2020

Quantitation of metallothionein mRNA by RT-PCR and chemiluminescence

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12 December 2020

Quantitative RT-PCR for measuring gene expression

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12 December 2020

Primary structure of the bacteriophage T4 DNA helix-destabilizing protein

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12 December 2020

Products of bacteriophage T4 genes 32 and 45 improve the accuracy of DNA replication in vitro

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12 December 2020

Redesigning trypsin: alteration of substrate specificity

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12 December 2020