Dr. Sergey M. Zaytsev Profile

Dr. Sergey M. Zaytsev

at Univ of Lorraine

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Author

Publications (8)

SPIE Journal Paper | 27 May 2023 Open Access

In vivo skin optical clearing efficacy quantification of clinically compatible agents using line-field confocal optical coherence tomography

Sergey M. Zaytsev, Marine Amouroux, Valery V. Tuchin, Elina A. Genina, Walter Blondel

JBO, Vol. 28, Issue 05, 055002, (May 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.5.055002

KEYWORDS: Skin, Mixtures, In vivo imaging, Optical clearing, Optical coherence tomography, Tissues, Confocal microscopy, 3D image processing, Permeability, Tissue optics

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Proceedings Article | 7 March 2023 Presentation + Paper

Characterization of human skin optical clearing based on confocal optical coherence tomography imaging and optical clearing agents for clinical use

Sergey Zaytsev, Walter Blondel, Marine Amouroux, Valery Tuchin, Elina Genina

Proceedings Volume 12378, 1237807 (2023) https://doi.org/10.1117/12.2649736

KEYWORDS: Skin, Optical clearing, In vivo imaging, Optical coherence tomography, Permeability, 3D image processing, Optical imaging

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Proceedings Article | 4 May 2021 Paper

Impact of ex vivo skin dehydration on collimated transmittance spectra kinetics

Sergey Zaytsev, Alexey Bashkatov, Walter Blondel, Marine Amouroux, Valery Tuchin, Elina Genina

Proceedings Volume 11845, 118450M (2021) https://doi.org/10.1117/12.2589589

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Proceedings Article | 10 October 2020 Presentation

Effect of probe pressure and drying on ex vivo skin UV-VIS-NIR spectroscopic signals

Sergey Zaytsev, Elina Genina, Alexey Bashkatov, Marine Amouroux, Walter Blondel, Valery Tuchin

Proceedings Volume 11553, 115531B (2020) https://doi.org/10.1117/12.2575195

KEYWORDS: Skin, Spectroscopy, Tissues, In vivo imaging, Collimation, Transmittance, Signal attenuation, Luminescence, Diagnostics, Tissue optics

Proceedings Article | 10 October 2020 Poster + Paper

Analysis of image features for the characterization of skin optical clearing kinetics performed on in vivo and ex vivo human skin using Linefield-Confocal Optical Coherence Tomography (LC-OCT)

Sophie Tran, Sergey Zaytsev, Viktoriya Charykova, Munira Yusupova, Alexey Bashkatov, Elina Genina, Valery Tuchin, Walter Blondel, Marine Amouroux

Proceedings Volume 11553, 115532P (2020) https://doi.org/10.1117/12.2575173

KEYWORDS: Optical coherence tomography, Skin, Image analysis, Optical clearing, Tissues, In vivo imaging, Skin cancer, Cancer, Tissue optics, Light scattering

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Proceedings Article | 2 April 2020 Presentation

Physical impacts on epidermal permeability in vivo for optical clearing agents (Conference Presentation)

Elina Genina, Alexey Bashkatov, Sergey Zaytsev, Marine Amouroux, Walter C. P. Blondel, Valery Tuchin

Proceedings Volume 11363, 113630P (2020) https://doi.org/10.1117/12.2555734

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Proceedings Article | 2 April 2020 Presentation

Optical spectroscopy as an effective tool for skin cancer features analysis: applicability investigation (Conference Presentation)

Sergey Zaytsev, Walter Blondel, Marine Amouroux, Grégoire Khairallah , Valery Tuchin, Elina Genina

Proceedings Volume 11363, 113631Z (2020) https://doi.org/10.1117/12.2555776

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Optical methods in medicine are exploit the light propagation through the biological tissue, to non-invasively extract morphological and geometrical parameters of the tissue under investigation, from the detected light. However, because the transport of photons into biological tissues of interest is weakened due to losses caused by multiple light scattering, absorption and reflectance, the depth resolution and photon penetration depth of these optical methods still need to be improved. The optical properties of biological tissues can be controlled using special biocompatible hyperosmotic agents or Optical Clearing Agents (OCA). The latter might penetrate inside a tissue, interact with and modify its natural high-scattering formation through different ways and thus, increase the photons penetration within the biological tissue. The aim of this work was to study the modifications in depth resolution of the proposed Spatially Resolved Multi-Modal Spectroscopy (SRMMS) method by combining it with an optical clearing approach and to estimate the applicability of this method for future clinical use. As an OCA, PEG-400/DMSO (80%/20% vol/vol) and Sucrose/PEG-400/PG (50%/45%/5% vol/vol) solutions were used. Depending on the experimental condition, either one of 2 OCA, or saline solution or nothing ("dry" condition) was applied on the skin surface of 2-layer hybrid model, made of skin layer on top and the gel layer, containing exogenous fluorophore Chlorin e6, on bot. The SRMMS probe made of one central excitation fiber and 4 rings of collecting fibers for collecting spectroscopic data with the separation by depth, was placed in contact with the skin at the area of application. Then, Diffuse Reflectance and excited on a different wavelengths (365,385,395, 405 and 415 nm) Autofluorescence spectra were aquired at initial time point, and then every 4 minutes within 36 minutes. Aquired DR and AF spectra were preprocessed, and the Area Under the Curve (AUC) values were calculated for the specific wavelength bandwidths of interest for the skin-AF and Ce6-fluorescence spectra obtained at every of the 5 excitation peaks. AUC values were normalized to the T0 respective values to get the time kinetics curves of the spectroscopic signals. Time kinetics of skin-AF and Ce6-fluorescence spectrum AUC showed that under 36 minutes of OCA application it is possible to observe certain enhanced light penetration effect through an increasing of normalized Ce6 AUC kinetics and a decreasing of normalized AF AUC kinetics. Thus, it was possible to observe that optical clearing led to increased depth resolution with time. Moreover, the effect of clearing itself was observed to be dependent on the chosen OCA, while the saline solution application didn’t cause significant changes in normalized fluorescence intensities. The result of “dry” condition experiment highlighted that while there were no significant changes in AF spectrum amplitudes, a strong increase of Ce6 fluorescence emission intensity was collected from the bottom gel layer. This is probably due to drying of an ex vivo skin sample and the applied probe pressure effect.

Proceedings Article | 3 June 2019 Paper

Research and development of effective optical technologies for diagnostics in dermatology

Vadim Genin, Prisca Rakotomanga, Sergey Zaytsev, Elina Genina, Ekaterina Lazareva, Grégoire Khairallah, Marine Amouroux, Charles Soussen, Hang Chen, Wei Feng, Dan Zhu, Alexey Bashkatov, Walter Blondel, Valery Tuchin

Proceedings Volume 11065, 1106505 (2019) https://doi.org/10.1117/12.2528700

KEYWORDS: Skin, Optical coherence tomography, Tissue optics, Luminescence, In vivo imaging, Scattering, Absorption, Optical clearing, Photons, Transparency

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