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Microscope Basics

This document provides instructions for using a microscope. It explains the basic parts of a microscope including the ocular lens, body tube, nosepiece, objectives, stage, stage clips, diaphragm, coarse and fine adjustments, light, arm, and base. It also discusses calculating magnification by multiplying the ocular and objective powers, making wet mount slides, and comparing images at different magnifications.

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157 views6 pages

Microscope Basics

This document provides instructions for using a microscope. It explains the basic parts of a microscope including the ocular lens, body tube, nosepiece, objectives, stage, stage clips, diaphragm, coarse and fine adjustments, light, arm, and base. It also discusses calculating magnification by multiplying the ocular and objective powers, making wet mount slides, and comparing images at different magnifications.

Uploaded by

Anonymous h2EnKyDb
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPT, PDF, TXT or read online on Scribd
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T.

Trimpe 2005
http://sciencespot.net/
Ocular lens
(Eyepiece)
Body Tube

Nosepiece
Arm
Objectives

Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light

Base
Always carry a microscope with one hand
holding the arm and one hand under the base.
What’s my power?
To calculate the power of magnification, multiply the power of the
ocular lens by the power of the objective.

What are the powers of


magnification for each of
the objectives we have on
our microscopes?
Fill in the table on
your worksheet.
Comparing Powers of Magnification

We can see better details with higher the


powers of magnification, but we cannot
see as much of the image.

Which of these images


would be viewed at a
higher power of
magnification?
Let’s give it a try ...
1 – Turn on the microscope and then rotate the nosepiece to click the
red-banded objective into place.
2 – Place a slide on the stage and secure it using the stage clips. Use
the coarse adjustment knob (large knob) to get it the image into view
and then use the fine adjustment knob (small knob) to make it
clearer.
3 – Once you have the image in view, rotate the nosepiece to view it
under different powers. Draw what you see on your worksheet!
Be careful with the largest objective! Sometimes there is
not enough room and you will not be able to use it!
4 – When you are done, turn off the microscope and put up the
slides you used.
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use
too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover Lower slowly
Slip

5 – Place the slide on the stage and view it first with the red-banded
objective. Once you see the image, you can rotate the nosepiece to
view the slide with the different objectives.
You do not need to use the stage clips
when viewing wet-mount slides!

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