USER GUIDE

37-228’03 REV A

ADP450 Series
Peltier Polarimeter
ADP450 User Guide (EN)
Code 37-228
Issue 03
Revision A
Date January 2019

Bellingham + Stanley, a Xylem brand, has been manufacturing high quality optical
instruments in the UK for over 100 years and is a leading manufacturer of
refractometers and polarimeters.

Our current range of products includes optical and digital hand refractometers as well
as a full range of laboratory refractometers and polarimeters available through a
network of trained distributors throughout the world. Process refractometers are also
available through specialist outlets.

Visit our website, www.bellinghamandstanley.com, for full details of Bellingham +

Stanley, our products and foreign language brochures.

Contact our Sales team to discuss a particular application or to receive details of your
local distributor.

Xylem Inc. recognise all trademarks

 2017 Xylem Inc.

Every effort has been made to ensure the accuracy of the contents of the manual.
However, Bellingham + Stanley Ltd. and Xylem Inc. can assume no responsibility for
errors contained in the manual or their consequences.

Printed in UK

Bellingham + Stanley Bellingham + Stanley

Longfield Road 90 Horizon Drive
Tunbridge Wells, Kent TN2 3EY Suwanee, GA 30024
United Kingdom United States of America
Main: +44 (0) 1892 500400 Main: (678) 804 5730
Fax: +44 (0) 1892 543115 Fax: (678) 804 5729
sales.bs.uk@xyleminc.com sales.bs.us@xyleminc.com
 Contents
Section 1: Installation ............................................................................. 3
Instrument overview ..................................................................... 4
Menu structure ............................................................................. 5
Unpacking .................................................................................... 7
Positioning the system ................................................................. 8
Mains connection ......................................................................... 8
Section 2: Instrument operation ............................................................ 9
Basic operation ........................................................................... 11
Setup Wizard .............................................................................. 14
Measurement Display................................................................. 15
Measurement settings ................................................................ 19
Calibration and Reading ............................................................. 25
Saving results ............................................................................. 29
Data Menu .................................................................................. 35
Section 3: Instrument Setup ................................................................ 40
Methods ...................................................................................... 41
User accessibility........................................................................ 43
Reading setup ............................................................................ 54
System settings .......................................................................... 67
Section 4: System maintenance .......................................................... 70
Maintenance Menu ..................................................................... 71
Information and Help .................................................................. 72
Setting default values ................................................................. 72
Section 5: Specification ........................................................................ 74
Specification ............................................................................... 75
Section 6: Accessories and Contact ................................................... 77
Accessories ................................................................................ 78
Contact ....................................................................................... 81

ADP450 37-228’03 Rev. A January 2019 Page 1

 Declaration of conformity
According to ISO/IEC 17050-1 & 2: 2004

Manufacturer's Name Bellingham + Stanley

Manufacturer's Address Longfield Road,
Tunbridge Wells,
Kent TN2 3EY
United Kingdom
declares that the product

Product Name ADP450 Series Polarimeter

Model Number All

Is designed to conform to the following Product Specifications:

Safety:
BS EN 60950-1:2002

Applied and met EMC test standards:

Emissions
EN 61326-1:2013
• Radiated emissions CISPR 11:2009 inc.
A1:2010, Class A
• Conducted emissions, ac port CISPR 11:2009 inc.
A1:2010, Class A
• Conducted emissions, ethernet port CISPR 11:2009 inc.
A1:2010, Class A
EN 61000-3-2:2014
• Mains harmonics Class A
EN 61000-3-3:2013
• Mains voltage flicker (dmax=4%)
Immunity:
EN 61326-1:2013 - Basic immunity requirement (Table 1)
• Electrostatic discharge EN 61000-4-2:2009
• Radiated RF interference EN 61000-4-3:2006 inc.
A1:2008 & A2:2010
• Fast transient bursts EN 61000-4-4:2012
• Surge EN 61000-4-5:2014
• Conducted immunity EN 61000-4-6:2014
• Voltage dips and interruptions EN 61000-4-11:2004

Supplementary:
The product herewith is designed to comply with the requirements of the EMC
Directive 2014/30/EU and the Low Voltage Directive 2014/35/EU.

This symbol is an internationally agreed indicator that the

product bearing it should not be disposed of as general
waste or garbage which might end up in landfill sites, but
should instead be sent for special processing and/or
recycling in those countries where appropriate legislation
and facilities are in place.

The symbols below are used throughout this user guide.

Caution or warning. Electrical hazard.

Hint or tip.

Page 2 37-228’03 Rev. A January 2019 ADP450

 Section 1: Installation
Instrument overview ............................................................................... 4
Menu structure ........................................................................................ 5
Unpacking ................................................................................................ 7
Contents list .................................................................................. 7
Positioning the system ........................................................................... 8
Mains connection .................................................................................... 8
Power requirements ..................................................................... 8
Warning ........................................................................................ 8

ADP450 37-228’03 Rev. A January 2019 Page 3

 Instrument overview
The ADP450 Series Peltier Polarimeter with patented XPC technology is
a self-contained, easy-to-use instrument suitable for measuring the
rotation of optically active samples.

The instrument is housed in a case which is light in weight whilst being

extremely rugged and has a high resistance to chemical attack from the
majority of commonly used samples.

Sample temperature is controlled by the Peltier Temperature control

system which enables rapid stabilisation and fulfils the temperature
requirements of all Pharmacopoeias.

Operation is very simple by way of four push buttons including scale and
compensation selection, zero calibration and the displaying of optical
density and temperature.

The instruments conform to a number of industry measurement

standards and offer operational features that allow use in an environment
controlled by FDA regulation 21 CFR Part 11. Built in RFID technology
helps provide clearance and a log of operator and configuration functions.

Readings can be printed via USB, serial or network ports. Alternatively

they can be stored in a database or as secure PDFs to later download via
either a USB removable storage device or across a local area network.

The ADP450 Series Peltier Polarimeters meet requirements of British,

United States, European and Japanese Pharmacopoeias

A wide range of accessories including printers, barcode readers and USB

keyboards are available.

Page 4 37-228’03 Rev. A January 2019 ADP450

Menu structure
1. Calibration 3. Setup 4. Maintenance
1. Span (Top
1. Methods 1. Add new… 1. Maintenance Report
Calibration)
2. Undo 2. Settings… 1. Use Methods 2. Backup to USB 1. Backup
3. Report 2. Hide preset 2. Frequency
4. Warning 3. Selection Type 3. Last Changed
2. Users 1. Add new… 3. Update Software
2. Data 2. Settings… 1. Protection 1. basic 5. cfr
1. Saved Results 2. low
2. System Log 3. medium 5. Help
3. Copy Files to USB 4. high 1. Setup Wizard
4. Move Files to USB 2. User Types 1. Add new… 2. Information
5. Export Logs to USB 2. administrator 3. Contact
3. calibrator 4. User Guide
4. operator 5. PC software
3. Reading 1. Response 1. fast (2s)
2. medium (20s)
3. slow (40s)
2. Scales 1. Add new… 1. From Library
2. From Data
3. Temp. Comps. 1. Add new… 1. From Data
1. none 5.
4.Recording 1. Mode
statistics
2. single 6. multi
3. save
4. single + save
1. none 5.
2. Printer
network
2. serial
3. usb
4. pdf
3. LIMS Output 1. Fields 1. serial
2. none 2. network
3. csv 3. usb file
4. xml 4. ftp file
4. Traceability 1. Location
2. Batch Codes 1. off
2. number
3. text
4. dateinc
3. Auto Increment 1. off
2. on
4. Log Users 1. off
2. number
3. text
4. login
5. Remember Text 1. none
2. batch
3. user
4. batch and
user
5. Ports 1. Serial Port 1. Baud Rate
2. Word Length
2. Network 1. DHCP
2. IP Address
3. Subnet Mask
4. Default
Gateway
5. System 1. Language
2. Time / Date 1. Date Format 1. dd/mm/yy
2. mm/dd/yy
2. Summer Time 1. no
2. yes
3. Time Server
4. Set Clock…
4. UTC Offset
3. Display 1. Brightness
2. Remote Display
3. Setup
4. Clone From Backup
5. Temperature Control 1. Mode 1. off
2. on

ADP450 37-228’03 Rev. A January 2019 Page 5

Page 6 37-228’03 Rev. A January 2019 ADP450
 Unpacking
Carefully remove all of the packing material. It is recommended that the
box and other packing materials are retained so that, should the need
arise, the polarimeter can be safely returned to the supplier for service.
Check that all parts listed below are present and that no transit damage
has occurred. If any are damaged or missing, contact the supplier
immediately.

Contents list

Quantity Item Code

1 ADP450 Series Polarimeter See below
1 Mains lead See below
1 Power supply 55-105
1 Quick Start Guide 37-234
1 Key-fob type ID tags (pack of 3) 22-071
1 200mm stainless steel tube 35-91
XPC-200 Adaptor for 200mm stainless steel
1 35-96
tube
1 Temperature probe sleeve 37-565

Instrument code numbers:

Model Complete polarimeter Polarimeter module only

ADP450 37-50 37-450

Mains lead code numbers:

Moulded plug type for Code

Switzerland 61-181
Denmark 61-182
India / South Africa 61-188
Australia 61-189
UK 13 Amp square pin to BS1363/A 61-191
United States (3 pin) 61-192
Europe (Schuko) 61-193

ADP450 37-228’03 Rev. A January 2019 Page 7

 Positioning the system
Place the instrument on a flat and stable bench that is:

• Dry and indoors.

• Capable of holding the instrument’s weight.
• Away from draughty or hot equipment like fans or heaters.
• Out of direct sunlight or strong ambient light.
• Away from potential sources of interference, such as RFI
generating equipment.
• Within reach of a power point.
• Not using a power circuit that also has large motors or noise
generating equipment connected to it.

Do not block the air vents.

WARNING:
• Disconnect all cables before moving.
• Ensure the chamber is empty before moving.
• Wear appropriate PPE for samples under test.

Mains connection
The power supply adapter is supplied with a moulded mains cord and
plug to suit one of several socket types. For UK leads, replace the fuse
only with the type indicated on the plug.

Power requirements
Voltage 110 to 230 V ~ ±10%
Frequency 50 to 60 Hz
Maximum current 2A

Warning
RISK OF ELECTRIC SHOCK:
• For indoor use only.
• Must be kept dry.
• Disconnect the equipment from the mains supply before
unplugging the mains lead from the instrument.

WARNING:
• Do not cover, designed to operate with free air convection.
• No cleaning required

Page 8 37-228’03 Rev. A January 2019 ADP450

 Section 2: Instrument
operation
Basic operation ..................................................................................... 11
Switching on and off ................................................................... 11
Manoeuvring through menus ..................................................... 11
Keying in letters, numbers and other characters ....................... 12
Keying in numerical values ........................................................ 13
Setup Wizard .......................................................................................... 14
Measurement Display ........................................................................... 15
Title Bar ...................................................................................... 15
Reading display and data ........................................................... 17
Softkeys ...................................................................................... 18
Measurement settings .......................................................................... 19
Changing settings ....................................................................... 19
Basic settings ............................................................................. 19
Scale........................................................................................... 19
Temp. Comp. (temperature compensation) ............................... 20
Tube Length ............................................................................... 20
Set Temp (temperature control set-point) .................................. 20
Advanced settings ...................................................................... 21
Stability ....................................................................................... 21
Limits .......................................................................................... 21
Resolution .................................................................................. 22
Multiplication ............................................................................... 22
Range ......................................................................................... 22
Using Methods ........................................................................... 24
Calibration and Reading ....................................................................... 25
XPC Temperature Adaptor ......................................................... 25
Zeroing the instrument ............................................................... 25
Taking a reading......................................................................... 26
Span calibration.......................................................................... 27
Undo calibration ......................................................................... 28
Calibration report ........................................................................ 28
Warning frequency ..................................................................... 28
Saving results ........................................................................................ 29
Set the print mode ...................................................................... 29
Printer ......................................................................................... 30
Network printer ........................................................................... 30

ADP450 37-228’03 Rev. A January 2019 Page 9

 LIMS output ................................................................................ 31
Traceability ................................................................................. 31
Location ...................................................................................... 32
Batch codes ................................................................................ 32
User codes ................................................................................. 32
Remember text ........................................................................... 32
Port settings ............................................................................... 33
Statistics print mode ................................................................... 34
Data Menu .............................................................................................. 35
The Reading Log ........................................................................ 35
Reading Log Options.................................................................. 35
Result Information ...................................................................... 36
The System Log ......................................................................... 37
Export Logs to USB .................................................................... 38
Downloading Files ...................................................................... 38
FTP access ................................................................................ 38
File Integrity ................................................................................ 39

Page 10 37-228’03 Rev. A January 2019 ADP450

 Basic operation

Switching on and off

Plug in the power supply, ensure the sample chamber is empty and
switch on the mains supply. Once loaded, the instrument will be in
standby mode. To switch on, press the left Power softkey. After a few
moments the instrument will show the Measurement Display (or the
Setup Wizard if switching on for the first time).

Place the adaptor on the instrument rails so that the flat surface is in
contact with the temperature controlled plate.

To switch to standby mode press and hold the Power Softkey for several
seconds and select Yes on the confirmation screen. It is then safe to turn
power off the instrument.

Manoeuvring through menus

The softkey functions change depending upon what inputs are required at
that time. If a softkey has no text then that button is disabled.

Menu items can be selected by either:

• Pressing the Up/Down softkeys until it is highlighted and then

pressing Select
• Pressing the item number key e.g. press 3 for Setup

ADP450 37-228’03 Rev. A January 2019 Page 11

 If the menu item contains a list of options to choose from then either:

• select the menu item and then choose the option from the
displayed list
• press the Forward/Back arrow keys to step through the options

Menu items that can be modified by using the arrow keys are identified by
arrows being displayed next to the menu items value.

Return to the Measurement Display by repeatedly pressing Quit to step

back through the menus.

Keying in letters, numbers and other characters

When it is necessary to enter in text, the alpha-numeric entry screen will
be displayed. This will allow entry of numbers, letters and a range of
punctuation characters by multiple presses of keys.

E.g. To enter the name cola(74):

• press the 2 key 3 times c

• press the 6 key 3 times o
• press the 5 key 3 times l
• press the 2 key 1 time a
• press the 1 key 10 times (
• press and hold the 7 key 7
• press and hold the 4 key 4
• press the 1 key 11 times )

Once a key is pressed the available characters that can be selected

using that key are shown at the top right of the screen (see the table
below).

If a key is not re-pressed within one second, the displayed character will
be accepted. Pressing and holding a number key will produce that key’s
number.

Page 12 37-228’03 Rev. A January 2019 ADP450

 Key Available characters
1 - ? ! . : ; “ ‘ ( ) 1

2 a b c 2

3 d e f 3
4 g h i 4

5 j k l 5

6 m n o 6
7 p q r s 7

8 t u v 8

9 w x y z 9
0 + $ % / # < = > _ 0

A USB keyboard, available from Bellingham + Stanley (code 55-86), can

also be used for text and number entry.

Keying in numerical values

Numerical values can be entered in either fixed decimal point format or in
scientific notation. E.g.:

Fixed decimal point Scientific

1.2345 1.2345E0
or
-123.45 -1.2345E2
0.00012345 1.2345E-4

The second softkey gives access to the minus (-), decimal point (.) and
exponent (E) symbols that are required to enter both numerical formats.
As a number is entered the instrument responds to the key strokes by
changing the softkey function.

If the number being entered is negative press the minus softkey before
entering the number. If the number is less than 1, press 0 first to show
the decimal point softkey. If an error is made while entering the number,
press the Clear softkey to erase the entry. The OK softkey may be
pressed at any time to confirm the entry.

ADP450 37-228’03 Rev. A January 2019 Page 13

 Setup Wizard
When switching on for the first time, after the instrument has completed
its startup procedures, the Setup Wizard will appear. First select the
desired language. A list of different options will then appear.

• Copy user guides and PC software to USB: Makes copies of

all user guides and PC software compatible with the instrument
to a removable USB storage device.
• Set the standard measurement conditions: Make changes to
the measurement settings, see Page 19.
• Set clock: Set the instrument to local time and set the date
format, see Page 67.
• Set the printer options: Specify how reading results will be
printed or saved, see Page 29.

When the desired conditions are set in each screen press the Quit
softkey to proceed.

Once all required settings are changed press Quit on the Setup Wizard
Menu to show the Measurement Display.

The Setup Wizard can be run at any time from the Help Menu.

Page 14 37-228’03 Rev. A January 2019 ADP450

 Measurement Display
From the Measurement Display readings can be viewed and saved.

Title Bar
The title bar contains several elements that will be displayed on every
screen.
3
1 2 4

1. Title: Current location within the instrument’s menu structure.

The colour of the title bar will change depending on the current operation.

Red: In the Main Menu structure. This indicates that changes will be
made to the instrument’s settings.

Orange: In Modes/Methods. Changes made will affect the measurement

settings.

Green: Associated with taking and initiating readings.

Blue: Within a process that affects how the current reading will be printed
or saved.

These colours are replicated on the associated Softkeys.

If security settings are in use, the username of a logged-in user will also
appear here.

2. Security status: A indicator for whether a User is logged in.

Users logged out, symbol orange User logged in, symbol green

To log a user out hold down the left arrow key whilst on the Measurement
Display.
ADP450 37-228’03 Rev. A January 2019 Page 15
 3. Temperature control status: Displays the stability status, set
temperature and current temperature. The colour and status symbol will
change depending on the set-point temperature and the measured
temperature.

• Temperature stable

The tick indicates that the measured temperature (second value) is stable
to within ±0.2°C of the set-point temperature (first value).

• Temperature stabilising

This will often occur when applying a fresh sample which has a
significantly different temperature to the set point.

The stability symbol oscillates to show that the temperature is not yet
stable.

• Temperature changing

This will occur when the temperature set-point is changed or after the
instrument is switched on.

The stability symbol becomes a cross and there can be a significant

difference between the measured temperature and the set-point
temperature.

4. System clock: Shows the current system time in a 24 hour format. To

alter the time, see Page 67.

If the system clock turns orange, it is to indicate that the calibration

warning has expired – it has been a certain number of days since the
instrument was last calibrated, see Page 28.

Page 16 37-228’03 Rev. A January 2019 ADP450

 Reading display and data

2
1

3
5
4

1. Reading result: Readings will be displayed here. The result will be

displayed in the units set in the Mode/Method, shown in the reading
configuration section of the Measurement Display.

If an article has been left in the chamber which totally blocks the light
path, the display will show all dashes.

2. Progress bar: Demonstrates the reading stability based on the

response time, see page 54. Not displayed if stability active, see page 21.

On printouts, the stability field has 3 possible values, below shows the
stability value and the associated on screen display.

Printout Screen Printout Screen Printout Screen

Ok Un No

Reading stable Not yet stable Light path blocked

3. Temperature: The temperature that the sample was at when the

reading was taken will be displayed here.

4. Optical Density: The optical density is a measure of the light and the
sample optical density (OD)

where:
OD = - Log10 ( % light transmission / 100 )

OD 0.0 = 100% light transmission, no absorption in the sample

OD 1.0 = 10% light transmission, 90% absorption in the sample
OD 2.0 = 1% light transmission, 99% absorption in the sample
OD 3.0 = 0.1% light transmission, 99.9% absorption in the sample

If the optical density of the sample is greater than 3, the background of

the reading area will appear grey.

A high OD measurement could be used to indicate that there is a bubble

in the polarimeter tube if the OD value is higher than typical for the
sample under test.

5. Measurement settings: The scale, temperature compensation and

tube length used for the reading.

ADP450 37-228’03 Rev. A January 2019 Page 17

 Softkeys

Zero button: Zeros the instrument, see page 25. Hold for several
seconds to switch the instrument to standby.

Record button: Pushing the button will record the result to the Reading
Log (Page 35) or printer depending on the print settings, see Page 29.

Mode button: Gives access to edit all Basic and Advanced reading
settings, see below. This will allow Method selection if Methods are
active, Page 41.

Menu button: Opens the instrument’s Main Menu from where all
instrument settings can be changed.

When working in CFR security level available softkeys will be different,

see page 45.

Page 18 37-228’03 Rev. A January 2019 ADP450

 Measurement settings
The measurement settings affect how a reading is collected and
presented. If different sets of measurement settings are required then
Methods can be enabled. For simplicity, the instrument is supplied in
Mode operation.

Changing settings
To change measurement settings press the orange Mode button. The
Mode Configuration is divided into two screens with Basic and Advanced
settings.

The Basic settings change the way a result is calculated, for example the
scale units and temperature compensation applied, and also allows
changes to temperature and wavelength. The Basic settings are:

• Scale, Page 19
• Temp. Comp., Page 20
• Tube Length (mm), Page 20
• Set Temp, Page 20

The Advanced settings allow the use of additional criteria to affect how a
measurement is taken and displayed. The Advanced settings are:

• Stability, Page 21
• Limits, Page 21
• Resolutions, Page 22
• Multiplication, Page 22
• Range, Page 22

Once set, press the Quit button to return to the Measurement Display.
Changes to the Basic settings will be displayed on the Reading
Configuration section of the screen.

Basic settings

Scale
By default the available scales are:

Scale name Scale ID

angular (°a) °a
iss (°z) °z

Many other pre-installed scales, as well as custom scales, can be added,

see Page 54.

ADP450 37-228’03 Rev. A January 2019 Page 19

 Temp. Comp. (temperature compensation)
By default the available temperature compensation modes are:

TC name TC ID
none nc
sugar sc
quartz qc

Additional temperature compensations can be added, see Page 65.

• “Sugar” temperature compensation

“Sugar” temperature compensation will correct readings of water and

sucrose solutions to 20°C. It conforms to the published ICUMSA
correction tables which covers the ranges 5 to 40°C. Although the
correction is specifically applicable to pure sucrose solutions, it is also
valid for many sugar based food products. However, it must be stressed
that the correction values may be unsuitable for other, non-sugar based,
products and great care should be exercised if “sugar” temperature
compensation is used with these samples.

• “Quartz” temperature compensation

“Quartz” temperature compensation will correct readings of quartz control

plates to 20°C in accordance with ICUMSA, it covers the range 5 to 40°C.

A variety of Quartz Control Plates are available from Bellingham +

Stanley, see the Accessories section.

Tube Length
Select the length of tube to be used for measurement. This will ensure
calculations are correct when using special scales and optimise
performance of the XPC temperature control system. Custom values can
also be set, contact Bellingham + Stanley for information regarding XPC
adaptors for different tube lengths. Select not applicable when working
with Quartz Control Plates.

Set Temp (temperature control set-point)

Acclimatise sample temperature prior to taking a reading for faster
stabilisation times. Ensure the correct tube length is selected.

The temperature control system in the instrument can be set from 15 to

35°C. In most situations, it is likely that all samples will be measured at
the same temperature; e.g. 20°C for British Pharmacopoeia or 25°C for
US Pharmacopeia.

Some manufacturers, who produce a range of different products for

different markets at one facility, may need to measure samples at more
than one temperature. However, temperature changes must be used with
care. Due to the time required for stabilisation it is not practical to
continually adjust the temperature set-point for every sample.

Page 20 37-228’03 Rev. A January 2019 ADP450

 Advanced settings
Advanced settings available will change dependent on any library scales
in use, See Page 55.

Stability
Stability sets conditions that must be met before a reading will be shown.
Stability can be set to:

• none
• delay
• smart

Delay causes a wait period before measurements are initiated. This

delay provides a fixed time for the sample to stabilise in the chamber and
to achieve thermal equilibrium throughout the sample mass.

SMART will request a temperature tolerance from 0.1 to 5°C then a time
in seconds up to 240. When active the instrument will check to make sure
the sample temperature is within the tolerance for the time specified
before a reading is shown.

Readings with Stability active will be initiated after the light path is
broken. To reread the same sample press the 1 softkey. The progress
bar will not be displayed once a reading with stability is taken.

Limits
Limits notify users if readings have fallen outside set criteria. If the
measured values fall outside the set Limit a line will be drawn through the
result. Limit acceptance will also appear on printed results.

The Limits can be set to check the reading value, temperature, OD or all
three.

Example: Limits have been set with the scale set to °a, with checks on
both reading and temperature:

Reading value: lower limit = 33.6 upper limit = 33.7

Temperature: lower limit = 19.5 upper limit = 20.5

ADP450 37-228’03 Rev. A January 2019 Page 21

 If the Reading value is lower than 33.6 or higher than 33.7, or the
temperature is less than 19.5 or higher than 20.5, then the reading will be
recorded as “fail” and displayed with a line through it.

Or a limit could be set on OD to detect bubbles in a sample tube, for

instance, as bubbles could cause a higher OD than is typical for a
sample.

Resolution
The reading can be displayed to 3 (high), 2 (medium) or 1 (low) decimal
places.

This can be set to be appropriate to the required accuracy for the sample
under test or scale type, for example specific rotation may only be
required to a low resolution according to certain Pharmacopoeia
Monographs.

Multiplication
The measured reading will be multiplied by this factor to create the
displayed reading.

For example, if a 100mm length sample tube is being used but the
equivalent result with a 200mm tube is required then enter a
multiplication factor of 2.

If the multiplication factor has been set to any value other than 1, the
scale identification field will show, for example, “Scale: °a x 2”, when a
multiplication value of 2 is used.

Range
The Range setting is only available when the angular degree scale (°a) is
selected. The default range for the °a scale is -89° to +89° (std). “(std)”
has been added to this default range title to allow easy identification of
the standard range.

Possible ranges are:

-359° to -181°
-269° to -91°
-179° to -1°
-89° to +89° (std)
+1° to +179°
+91° to +269°
+181° to +359°

Page 22 37-228’03 Rev. A January 2019 ADP450

 All polarimeters measure the amount of rotation of a plane of polarised
light and so it is only possible for the instrument to detect the position of
the rotated light beam over a 180° span. As shown in the diagrams
below, the plane of light leaving a sample with a rotation of +110° is
exactly the same as that from a sample with a rotation of -70°.

Therefore, a sample with a rotation of +110° will display -70° on the

default range.

0° Rotation 110° Rotation -70° Rotation

If the user is aware that the sample to be measured should have a

rotation greater than +89° then the correct value can be calculated from

180 + Displayed Rotation

Alternatively, if the +1° to +179° (or the +91° to + 269°) scale is selected,
then the correct rotation will be displayed.

If, in the above example, the user is unsure whether the correct rotation
is -70° or +110°, then an additional measurement should be taken with a
different length of tube (e.g. half the length of the first tube). If the original
true rotation was -70° then the second reading will be -35°. Otherwise, if
the original rotation was +110°, then the second reading will be +55°.

If any range other than -89° to +89° is selected, then the scale will be
identified as “°a + r”.

If a Multiplication factor is also in use, then the scale code will be shown
as, for example, “(°a+r) x 2”.

ADP450 37-228’03 Rev. A January 2019 Page 23

 Using Methods
A Method is a named set of measurement settings. They include all the
settings necessary for the instrument to take and check a particular
sample.

If the complete range of product types that is to be measured with the

polarimeter requires the same measurement settings then using the
Mode Menu is most suitable.

However, if a number of products with different reading requirements are

to be read then a Method can be added for each of them. For example, a
range of essential oils could be regularly measured and the display can
show whether each product is within its set tolerance. Alternatively,
Methods may be created to fulfil different Pharmacopoeia requirements
or Monographs.

An additional benefit of Methods is that, whilst the Mode/Method access

User right (Page 51) will allow users to change measurement settings
when operating with the Mode Menu, Methods access only allows for
change of Method. Only users with the higher Setup security rights are
able to change the measurement settings of a Method. This will ensure
an analysis is performed the same way every time.

To enable the use of Methods see Page 41.

The “normal” Method will use the same settings as those specified in
Mode.

Page 24 37-228’03 Rev. A January 2019 ADP450

 Calibration and Reading
Allow the instrument to stabilise for at least 30 minutes after switching on
before taking accurate readings.

XPC Temperature Adaptor

It is best to leave the temperature adaptor in place at all times to ensure

continued temperature stability. Just remove the tube when changing
samples. The adaptor should only be removed if different tube types or
Quartz Control Plates are to be measured.

The adaptors will sit on the polarimeter rails. Ensure the flat surface of
the adaptor is in good contact with the flat surface of the temperature
control system.

Zeroing the instrument

The instrument should be Zeroed regularly to ensure accurate readings.
The Zero can be performed with an empty chamber or adaptor, or a tube
filled with the solvent to be used to dissolve the sample.

If using a polarimeter tube, ensure that the cover glasses are clean, dry
and not over-tightened. If the Zero is to be performed with additional
solvent, make sure there are no air bubbles in the tube that could block
the light path by gently rocking the tube from end to end until they are
released through the filling tube. Open the sample chamber by lifting the
lids and place the tube on the adaptor. Close the lids.

To Zero the instrument press the Zero button on the Measurement

Display. This will cause the instrument to display all dashes briefly to
identify that the zero has been carried out.

ADP450 37-228’03 Rev. A January 2019 Page 25

 Taking a reading
If the instrument was Zeroed with an empty tube, add the sample to be
measured into the tube used until that the neck of the tube has filled
slightly. Gently rock the tube from end to end to ensure all air bubbles are
released through the neck.

If the tube used to Zero the instrument was filled with solvent empty the
solvent through the neck. Partially fill the tube with the sample to be
measured and rock the tube back and forth so the sample thoroughly
rinses the entire tube. Empty the tube, repeat this cleaning process, and
then add sample until the neck has filled slightly.

Do not readjust the end caps, this could change the stress on the
cover glass and slightly affect the optical rotation of the tube.

Lift open the chamber covers, place the sample tube on the adaptor and
place the temperature probe in the tube neck. Ensure that the
temperature probe is suitably immersed. Close the lids.

With stability set to none, the instrument will start taking readings
continually. Note that the progress bar will slowly fill, an indication that the
reading has yet to stabilise, and the reading result is displayed in grey.

When temperature and readings have stabilised the progress bar will
appear full and the reading will be displayed in black.

Page 26 37-228’03 Rev. A January 2019 ADP450

 If using temperature compensation the reading will be displayed black
before the temperature has stabilised.

If stability settings are active a single reading will be displayed once the
criteria are met. To take another reading of the same sample press the
“1” softkey.

The reading will be displayed using the scale and temperature

compensation set in the Mode menu or current Method, shown on the
Reading Configuration Panel.

Span calibration
Regular calibration is required to maintain instrument accuracy.

Span calibration sets the high end of the measurement range, performed
using a Quartz Control Plate available from Bellingham + Stanley.

To perform a Span press Menu, and then select:

• 1. Calibration
• 1. Span (Top Calibration)

The settings in Mode will be used unless Methods are active. The Span
method will then be automatically selected when a Span is being carried
out. Only °A and °Z scales can be used.

The instrument stores the details of the last sample used for Span
calibration. If a different QCP is to be used press the Change Sample
button and enter the data as requested, the sample value must be
entered in the scale used for calibration.

Confirm when the QCP has been placed in the chamber and the
instrument will perform all required measurements before setting the
Span.

The span result will be displayed on the Measurement Display until the
light path is broken.

If a Quality System is in place that requires regular calibration the

instrument can be set to give periodic warnings.
ADP450 37-228’03 Rev. A January 2019 Page 27
 Undo calibration
If a calibration was performed in error or, for example, the wrong
calibration sample was measured by mistake the last performed
calibration can be undone with this option.

Calibration report
The calibration report shows the details of the last Span. This includes
the date, time and measured value. The calibration report can also be
printed.

Warning frequency
Quality Systems and Good Laboratory Practice may require frequent
calibration of instrumentation. To assist with this, the instrument can give
a warning when it has not been Spanned within a certain number of days.

To set, select the Warning Frequency option and type the number of days
required. Set the value to 0 to turn off warnings.

When the warning period expires the clock on the Measurement Display
will turn orange.

A warning will also be displayed prior to each print attempt.

Page 28 37-228’03 Rev. A January 2019 ADP450

 Saving results
To access the Recording Menu to set up printing and saving, press Menu
then select:

• 3. Setup
• 4. Recording

Set the print mode

The print mode (option 1) has several different settings:

Mode Function

The Record button will be disabled preventing results

none
from being printed or saved.

single Print the last displayed reading to the selected printer.

Save the last displayed reading to the Reading Log

save
(see Page 35).

Print the last displayed reading to the selected printer

single + save
and save it to the Reading Log.

Allows a group of up to 10 measurements to be

statistics recorded and printed with a statistical analysis of the
data (see Page 34 for a description of the process)

Allows continuous measurements to be recorded with

a time delay between each. Press the Record button
then specify the total duration for readings (up to 1440
multi
minutes) and then interval between each reading (up
to 300s). After each reading the instrument will
automatically print the result to the selected printer.

ADP450 37-228’03 Rev. A January 2019 Page 29

 Printer
Several different printer types are available from Bellingham + Stanley,
see the Accessories section. The print format is set in option 2 of the
Recording Menu.

Printer Description

Result will not be sent to a printer, this format can be

used if only CSV results are required. Results will also
none
be stored in the Reading Log if the print mode is set to
single + save.
Readings can be sent to a terminal program or serial
serial
printer.
For printing to a PCL compatible printer connected via a
usb
USB port.
The instrument will save a PDF of results to memory
which can be later downloaded via FTP or removable
pdf
USB storage device, see Page 38. PDFs are secure to
prevent any alteration.

Result can be sent to a printer connected to a local area

network
network.

To set up a serial printer a USB FTDI serial adaptor, available from

Bellingham + Stanley (Code 55-85), will be required.

Select serial in the printer options. If a USB serial adaptor is already

connected to the instrument it will need to be removed.

Plug a USB serial adaptor into the instrument’s USB port and select ok.
The instrument will indicate if the adaptor has been detected, continue on
to set the baud rate and word length (9600 and 8 bit NP for printers
supplied by Bellingham + Stanley). Plug the serial printer into the other
end of the USB serial adaptor and results can then be printed.

A USB hub, available from Bellingham + Stanley (Code 55-88), will

support multiple USB serial adaptors. This allows a result to be recorded
to a LIMS and by any selected printer simultaneously.

For GLP the use of serial dot matrix printers is recommended.

Network printer
To print results to a printer on a local area network via the Ethernet port,
from the Recording Menu select Printer then option 5, network.

The option to enter the IP Address of the desired printer is then given.

Page 30 37-228’03 Rev. A January 2019 ADP450

 The cursor will skip to the next number box once a valid three digit
number is entered, or the Next button can be used to advance.

Press OK at any time to confirm the IP address. The entry will also be
accepted once all four numbers blocks are input.

LIMS output
Readings can also be printed as CSV (comma separated values) or XML
format, suitable for connection to a LIMS.

After selecting csv or xml formats, the output method can then be
selected.

In serial mode, readings will be sent to the serial port and can be
received by a proprietary terminal program or custom software.

With LIMS output set to network, readings can be sent via the Ethernet
port to a program capable of receiving UDP (user datagram protocol)
transmissions.

In usb file mode, a file is created on a connected USB removable storage

device.

The ftp file option will save a file in the instrument’s memory, allowing it to
later be transferred via FTP, see page 38.

When LIMS Output is active, a result is sent every time the Record button
is pressed on the Measurement Display. This allows a result to be
recorded to a LIMS and by any selected printer simultaneously.

Results in the Reading Log are also stored in CSV form and can be
downloaded separately, see Page 35.

• Select Fields

A lot of data can be associated with any individual reading. Depending on

regulatory standards under which the instrument is operated, all of this
data may be required or very little. The ability to define what fields are
sent in CSV LIMS outputs is given to limit redundant information.

Available column headers are grouped as types (e.g. instrument

information, reading information, traceability) to facilitate selection.

Traceability
All printed and saved readings are recorded together with the
measurement settings selected and the time and date of the
measurement. In addition, an optional product batch code and a user
code can be entered.

To setup traceability, press Menu then select:

• 3. Setup
• 4. Recording
• 4. Traceability

If either batch or user codes are enabled the instrument will request them
at the start of a print.

ADP450 37-228’03 Rev. A January 2019 Page 31

 Location
Specify where an instrument is located. This can be useful if results are
obtained from multiple instruments in different departments or different
facilities around the world.

Batch codes
The instrument can request batch codes in the following formats:

Setting Description

off The instrument will not request a batch code.

number Number from 0 to 32000.

text 14 character alpha-numeric string.

The date followed by an auto increasing number from 0 to

9999, in the format of: yymmddnnnn
dateinc
Example: 1703117235 (11th March 2017, number 7235)

If number is selected, an Auto Increment option is available which will

then offer a batch code which is one greater than the previously used
batch code when a reading is requested.

If text is selected, a barcode scanner can be used to input values. When

the batch code is requested simply scan the barcode.

Compatible barcode scanners are available from Bellingham + Stanley,

see the Accessories section.

User codes
The instrument can request user codes in the following formats:

Setting Description

off The instrument will not request a user code.

number Number from 0 to 99.

text 24 character alpha-numeric string.

Requires the user to login, see Page 43 for User

login Accessibility. The Signature set in the user’s profile will
then be used as the user code.

Remember text
If this option is set, the instrument will display the last entered batch or
user code in the input box when text Traceability is required.

Page 32 37-228’03 Rev. A January 2019 ADP450

 Port settings
The settings of both the serial and network ports can be altered from the
Recording Menu using option 5, Ports.

The USB Serial Port enables CSV printing to a LIMS, remote operation
via a terminal program or, if required, connection to PC software. A USB
FTDI serial adaptor is available from Bellingham + Stanley (Code 55-85).

The USB serial adaptor will be detected automatically unless a Serial

printer is assigned to the USB port.

• Serial Port

The baud rate and word length of the serial port can be altered to suit the
receiving device.

The default settings are:

Baud rate: 9600 Word length: 8 bit, no parity

These settings are those used by serial printers provided by Bellingham +

Stanley, see the Accessories section.

• Network communications

By default, the instrument uses DHCP to obtain an IP address. IP

address, Subnet Mask and Default Gateway can be changed as required
by first disabling DHCP. The instrument must be powered down for
changes to network settings to take effect.

To quickly view the current IP address of the instrument from the Main
Menu select:

• 5. Help
• 2. Information
• 3. Network

ADP450 37-228’03 Rev. A January 2019 Page 33

 Statistics print mode
If the print mode (see Page 29) is set to statistics, a group of up to 10
readings can be temporarily logged together and a statistical analysis
carried out upon the results.

To perform a statistical reading set, prepare and read a sample as

normal. After the reading is complete press the Record button and enter
traceability data as required. The statistics screen will then be displayed.

1
2

4 5 6

• 1. Traceability details.
• 2. Result counter – up to 10 readings can be used in each set of
statistics.
• 3. Statistical analysis of readings.
• 4. Clear button: Clears the currently stored statistics.
• 5. Print button: Prints the statistics and individual readings to
the set printer. The statistics are also cleared.
• 6. Next: Return to the measurement display to take the next
reading.

Page 34 37-228’03 Rev. A January 2019 ADP450

 Data Menu
The instrument has two separate logs for storing data. This data will be
held in the instrument whether the power is on or off. The logs can be
accessed by pressing the Data button on the Measurement Display or via
the Main Menu.

The Reading Log

If the print mode is set to “save” or “single + save” then pressing the
Record button will save the last displayed reading to the Reading Log.
10000 readings can be stored in the log.

On selecting Saved Results in the Data Menu the viewer will be

displayed.

Reading Log Options

• Result Information

Shows full result details and opportunities to sign and print the selected
result.

• Sort order

Choose whether the newest or oldest results are displayed at the top of
the Reading Log.

• Filter

The Reading Log can be filtered by:

Start/End Date: gives results from the set time period.

User: gives results by an individual user, user codes must be set to login,
see Page 32.
Batch: a text search if batch traceability is active.
Method: gives readings that were taken with a particular measurement
Method only.
Signed status: displays results that have or have not been signed at the
approval level of the current user.
Exported Status: displays results that have or have not been previously
exported from the instrument in some manner.

With a filter applied only those that meet the criteria will be shown in the
viewer, exported or cleared.

If user codes are set to login results from an individual user can be
displayed and then exported by scanning that user’s RFID tag. Scan
again to remove filter.

• Saved Results Printer

Results can be re-printed in the event that original printouts are lost. The
printer for this is set here. Available printers are the same as those for
printing readings, see page 30. This can be performed by using the Print
button when viewing the individual reading. If the reading has been
signed, signatures will be shown on this print.

ADP450 37-228’03 Rev. A January 2019 Page 35

 • Export Data

Press to export the filtered results in the format displayed on the option.
Results can be exported in CSV or PDF formats to USB drive or FTP.
This is defined in the Export Settings option (below).

• Export Settings

Format: This sets the file format that results will be exported in when the
export option (above) is selected.
Destination: This sets the destination media that results will be exported
to when the export option (above) is selected.
FTP Scheduled: Regularly exports the complete log to the instrument’s
FTP memory, this can then be downloaded to a PC or network location -
automatically with proprietary software. Only items that have not
previously been exported are transferred using this method.
Clear after Scheduled: Choose whether records are left in the Log after
a scheduled export is completed.
Last Export: Displays the data that the data was last exported

• Clear Results

This will delete the results that meet the conditions of the current filter
from the instrument.

If a result has not been previously exported in any format it will not be
deleted.

Result Information
Measurement details: The reading result

Print softkey: press to reprint this result. The printer type used can be
different to the one set in the main Recording Menu, set in the Reading
Log Options. Signatures will be included in these prints.

Signatures softkey: certain regulatory systems require electronic

signatures to submit and approve results. Press the button to access
signing. There are three levels of signature - Approver, Reviewer and
Submitter - each with the ability to accept or reject the result along with a
comment, for full details see Page 50.

Page 36 37-228’03 Rev. A January 2019 ADP450

 Configuration: the Measurement Settings active when the reading was
performed.

Device information: identifies the instrument used for the reading.

Calibration details: the last performed calibration at the time of reading.
Signatures: Indicates if a result has been signed as pass or fail by users
of different review levels.

The System Log

The System Log maintains a record of all changes and adjustments
made to the instrument by its users. This will include each Zero and Span
calibration, changes to the setup, configuration and Methods. Up to
10000 entries can be stored, further operation will be disabled until the
data is downloaded.

The System Log functions in the same way as the Reading Log with the
following filters available:

Event type
User
Date range
Exported status
Comment

Comments can be added to individual events, events with and without

comments can be filtered. All filtered data will be downloaded on export.
ADP450 37-228’03 Rev. A January 2019 Page 37
 Export Logs to USB
For convenience, Option 5, Export logs to USB, in the Data Menu will
download both Logs in full to a USB removable storage device in CSV
format.

Downloading Files
If the print format is set to PDF or LIMS Output to FTP (see Page 30),
every time a reading result is recorded or if a calibration report or Method
list, for example, is printed, a file is created and stored within the
instrument’s memory.

In order to access the files, they can be downloaded either via a

removable USB storage device or via FTP if a network is available.

PDFs are encrypted to prevent any alteration and are ideal for
transferring and storing data securely.

Downloading files to a USB removable storage device can be done in the

Data Menu. Copy files (option 3) will make a copy of the files currently
stored in the instrument but also leave copies in the instrument.

Move files (option 4) will download any files in memory to the USB
removable storage device and remove them from internal instrument
memory.

If more than one USB removable storage device is detected the

transfer will not be performed.

If a large number of files are copied or moved this process can take
several minutes, do not remove the USB removable storage device
during file transfer as this can lead to loss of data.

FTP access
If a large number of files have built up in the instrument, transfer via USB
could take several minutes. In this case it may be preferable to transfer
from the instrument via FTP.

Transfer could also be automated using proprietary software.

To view the instrument’s current IP address, press Menu then select:

• 5. Help
• 2. Information
• 3. Network

The current IP address can then be seen under the Network heading.
The network configuration for the instrument can be changed, see Page
33.

To view the files from a Windows computer open Windows Explorer, then
in the address bar type “ftp://username@XXX.XXX.XXX.XXX/” (without
quotes), where username is replaced by the username of a user with
Data access rights and the Xs represent the numbers in the instrument’s
IP address e.g. ftp://administrator@101.76.202.56/

Page 38 37-228’03 Rev. A January 2019 ADP450

 A log on will be required using the details of the user even if the
administrator PIN is set to 000 in the instrument.

With access to the instrument’s FTP server, images can be assigned to

individual Methods for ease of identification. PDF headers can also be
changed. Contact Bellingham + Stanley for details.

File Integrity
When PDFs or LIMS data is created in the instrument’s memory an MD5
checksum file is also created. Once files are downloaded to PC the MD5
File Verifier software, available from Bellingham + Stanley, can be used
to generate another checksum. This will be compared to the originally
created checksum file to ensure the downloaded files maintained their
integrity.

ADP450 37-228’03 Rev. A January 2019 Page 39

 Section 3: Instrument Setup
Methods .................................................................................................. 41
Hide preset ................................................................................. 41
Selection type ............................................................................. 41
Print List ..................................................................................... 42
Adding a new Method................................................................. 42
Editing a Method ........................................................................ 42
User accessibility .................................................................................. 43
Preset protection levels .............................................................. 43
FDA regulation 21 CFR Part 11 ................................................. 45
Security features ........................................................................ 46
User Types ................................................................................. 49
Approval Levels .......................................................................... 50
Users .......................................................................................... 51
Print User Lists ........................................................................... 52
Logging in/out ............................................................................. 52
RFID Tags .................................................................................. 53
Reading setup ........................................................................................ 54
Reading response ...................................................................... 54
Adding extra scales .................................................................... 54
Adding a library scale ................................................................. 55
Specific rotation .......................................................................... 56
Concentration ............................................................................. 57
% Inversion of sucrose solution ................................................. 58
% Change of product over a period of time................................ 59
Specific rotation with a known moisture content ........................ 60
Purity .......................................................................................... 61
Advanced Purity Settings ........................................................... 62
Editing the Purity equation ......................................................... 62
Connection to an RFM ............................................................... 63
Adding a custom scale ............................................................... 65
Adding a custom temperature compensation ............................ 65
USB and network scales ............................................................ 66
System settings ..................................................................................... 67
Language ................................................................................... 67
Time/Date ................................................................................... 67
Display ........................................................................................ 68
Temperature control ................................................................... 68
Clone settings ............................................................................. 68

Page 40 37-228’03 Rev. A January 2019 ADP450

 Methods
A Method is a defined set of measurement settings. This allows for
measurement settings to be quickly changed between frequently used
configurations or for reading different products with specific test
requirements. For example, if a range of different essential oils is
regularly measured a Method could be created for each with the display
set to show whether each product is within its set tolerance using the
limits system. Alternatively, Methods may be created to fulfil different
Pharmacopoeia requirements or Monographs.

Initially there are two Methods set in the instrument:

• normal for measuring samples

• span scale and temperature compensation used for
the Span calibration

The “normal” Method settings will match those configured in the Mode
Menu. Only the scale and temperature compensation can be set in the
“span” Method, the temperature and resolution will be the same as the
Method used prior to the calibration, other advanced settings are
preconfigured.

To activate Methods press Menu, then select:

• 3. Setup
• 1. Methods

Then set Use Methods to yes. This makes the Hide Preset, Selection
Type and Print List options available.

Hide preset
This will hide the two default Methods, “normal” and “span”.

If no custom Methods are added then the Methods button will be disabled
on the Measurement Display. The settings in the “normal” Method will
always be used for readings, this will help to ensure the measurement
settings are not changed.

Alternatively, if a number of Methods are created for a standard range of

products, e.g. mint oil, lavender oil, orange oil, and the preset Methods
will never be required, then it could be advantageous to hide the default
Methods from the Methods list. This will make it easier to select the
Method required.

Selection type
Before reading a sample, the relevant Method should be selected by
pressing the Method button on the Measurement Display.

This will show a list of all available Methods and the appropriate one can
be selected. However, if a large number of Methods have been created
then it could be easier to select the required Method by its index number
rather than scrolling through the list. The instrument therefore gives list
and number selection type.

ADP450 37-228’03 Rev. A January 2019 Page 41

 List selection type Number selection type

Holding down the Enter button for several seconds when the Method is
highlighted will enable editing of the Method, a login from a user with
Setup rights will be required if PINs are active.

Print List
This will print a list of all Methods to the set printer, providing a useful
reference if a large number of Methods are in use.

Adding a new Method

With Methods switched on, pressing Quit from the Method Settings menu
will show a list of current Methods. This list will be shown first when
re-entering Methods at a later time.

To add a new Method, select the Add new... option.

Enter the Method name, this could be a common name, such as mint oil
or orange oil, a trade name or a product reference code. Do not use a
batch code. Method names should identify a generic product type.

The Basic and Advanced measurement settings can then be configured

as required for the product type the Method will be used for. See Page 19
for a description of each of the measurement setting options. The starting
settings will be the same as those in the “normal” Method.

Once the Method settings are configured as required press Quit to return
to the Method list, from where more Methods can be added.

Editing a Method
There are several ways to edit a Method’s configuration.

From the Method List in the Setup menu simply select the Method which
requires editing and then change the settings as desired. Accessing the
Method this way is the only way for a Method to be deleted (or hidden on
higher security levels).

Methods can also be edited from the Select Method screen, accessed by
pressing the Method button on the Measurement Display. This is
dependent on selection type.

With List selection type press and hold the method number or highlight
the method and hold select. With number selection type, type the Method
number then press and hold Enter.

If PINs are active then a login by a user with Setup rights will be required
before the Method configuration can be accessed.

Page 42 37-228’03 Rev. A January 2019 ADP450

 User accessibility
RFID tags offer rapid user clearance, see Page 53.

Access to the instrument’s functions and Menus can be restricted, with

the sophistication of the protection set to match the operating
environment.

There are a range of security features that can be activated to preset

levels for convenience or customised to user preference.

It is recommended that the CFR level of preset protection settings is used

if working in a FDA controlled environment (e.g. 21 CFR part 11).

To access the Users menu press Menu, then select:

• 3. Setup
• 2. Users

It is important to set the level of security required before adding users as

some changes require the deletion of custom users to maintain a secure
environment.

The level of security can be set from the Protection Menu, found by
pressing the Settings button at the top of the Users menu. There are five
security levels. The basic level is feature limited to simply prevent Menu
access. Four other preset levels – low, medium, high and CFR – each of
which can be edited for the environment the instrument is used in, take
advantage of multiple users and gives access depending on
responsibility.

Preset protection levels

To set the protection level select the Settings option in the Users menu,
then select Protection.

• Basic

Basic will limit the instrument to two PINS, the "operator" PIN and the
"administrator" PIN. The "operator" PIN allows access to the Mode Menu
or Method Selection and Zero calibration. The "supervisor" PIN provides
unrestricted access to all menus. Each PIN is a 3 digit number in the
range 001 to 999.

With a PIN set, after attempting to access a Menu the user is prompted to
enter the PIN and press Enter.

Setting the "operator" PIN to 000 will give unrestricted access to the
Mode Menu/Method Selection and Zero calibration.

Setting the "administrator" PIN to 000 will give unrestricted access all
menus.

This level is suitable to restrict Menu access to prevent accidental

changes but may not be suitable when working in an environment subject
to stronger security regulations.

ADP450 37-228’03 Rev. A January 2019 Page 43

 The following preset protection levels use default configurations of the
security features as detailed on Page 46. The security levels can be
customised if desired.

• Low

The instrument will request a PIN when entering the Menu or

Mode/Methods.

Security feature Setting

User Selection PIN only
PIN length 3
Keep Logged In 30 secs
Maximum PIN Age Off
Remember Last User No
Reset PIN Only No
Prohibit PIN Recycling No
User Lockout No
Fast PIN Change No

• Medium

This preset improves security by requiring the user to enter their

username and then their PIN. This preset does not have features enabled
that could increase administration overheads.

Security feature Setting

User Selection User and PIN
PIN length 4
Keep Logged In 30 secs
Maximum PIN Age off
Remember Last User 10 mins
Reset PIN Only no
Prohibit PIN Recycling no
User Lockout no
Fast PIN Change no

Page 44 37-228’03 Rev. A January 2019 ADP450

 • High

This preset security level ensures PINs are regularly changed.

Security feature Setting

User Selection User and PIN
PIN length 4
Keep Logged In off
Maximum PIN Age 120 days
Remember Last User 10 mins
Reset PIN Only yes
Prohibit PIN Recycling 10
User Lockout 5
Fast PIN Change yes

• CFR

This preset features all the security options set to recommended levels
required when using the instrument in a FDA controlled environment.

Security feature Setting

User Selection User and Complex Password
PIN length 8
Keep Logged In off
Maximum PIN Age 120 days
Remember Last User 10 mins
Reset PIN Only yes
Prohibit PIN Recycling 10
User Lockout 5
Fast PIN Change yes

FDA regulation 21 CFR Part 11

This instrument complies with the technical aspects of FDA regulation 21
CFR part 11. However the instrument capabilities are only a small part of
compliance, standard operating procedures are required.

To setup the instrument to be used in a 21 CFR Part 11 environment the

following steps are required.

• Set the Protection level to CFR.

• Set user Traceability to login and an appropriate batch
traceability (Page 31).
• Set the appropriate Print Mode (Page 29). PDFs are stored
securely so that they cannot be altered once created. Saved
results in instrument memory cannot be changed. However, once
downloaded from the instrument appropriate measures must be
taken to prevent tampering. LIMS output enables direct
connection to a LIMS. Formats must be tested to ensure all
required data is available.

ADP450 37-228’03 Rev. A January 2019 Page 45

 • Set the instrument to operate with Methods (Page 41).
• Reset the administrator user PIN.
• Login as administrator and set a new password.
• Add all necessary User Types (Page 49) and Users (Page 51).
Reactivate the operator and calibrator accounts if required.

When CFR level is activated, Methods, Users and User types cannot be
deleted, only hidden, to prevent their reuse with different settings or the
same signatures being assigned to different users.

The instrument will now have a Read softkey to activate “single-shot”

readings only. Results will be automatically recorded using the selected
print mode after reading is complete.

Security features
The preset protection levels use the following settings:

• User Selection

There are four options for user selection:

PIN only
user and PIN
user and password
user and complex password

With “PIN only”, when a login is required, the user will only have to type in
a PIN. The length of the PIN can be set separately. This is used by the
low preset level.

Several features will not work when User Selection is set to PIN only as
they require two identifiers.

If using “user and PIN”, the user will first have to type in a username and
then a PIN. This is more secure than PIN only as two identifiers are
needed. Medium and high security levels use this setting.

With “password” characters from the instrument’s full alphanumeric

keyboard can be used, this can be more secure but could lead to more
entry errors.

Page 46 37-228’03 Rev. A January 2019 ADP450

 If “complex password” is selected, then each password will need to
include at least one of each of:

Lowercase letter
Uppercase letter
Number
Symbol (-,?,!,$ etc.)

A USB keyboard, available from Bellingham + Stanley will facilitate use

when using “Password”

• PIN Length (3 to 8 digits / 6 to 15 characters)

The greater the number of digits, the lower the chance of a PIN being
guessed. However, as the PIN length increases it will be harder to
remember.

With Passwords this specifies the minimum number of characters that a

user’s Password must be.

This should be set before adding custom users as they will be

deleted if this setting is changed.

• Keep Logged In (0 (off), 30 to 300 seconds)

This option specifies how long a user is kept logged in for whilst idle
before automatically being logged out. If set to 0, a user will be logged
out on returning to the Measurement Display.

Leaving the instrument logged in can lead to security issues as

unauthorised use of the instrument could occur. Therefore ensure that
the “Keep Logged In” feature is set appropriately for the instrument’s
working environment.

The instrument will return to the Measurement Display and log the user
out if they are idle in a Menu for 1 minute if set to 0. Otherwise the set
time will be used.

• Max PIN Age (0 (off) to 366 days)

This specifies how often a PIN must be changed. If the PIN is not
changed within this period the account PIN will be reset and a new PIN
requested on next login attempt.

• Remember Last User (0 (off) to 720 mins)

This option specifies how long the instrument will remember the last user
for.

If set, the instrument will display the last entered username in the input
box when a login is required. If the same user is logging in they will not
have to re-enter their username, only their PIN.

• Reset PIN Only (no / yes)

If selected, an administrator will only be able to reset a user’s PIN rather

than changing it. When a PIN is reset the next time the user logs in they
will be requested to set a new PIN.

If an administrator resets a user’s PIN the user should be present so that

they can create a new PIN for their username immediately.

ADP450 37-228’03 Rev. A January 2019 Page 47

 • Prohibit PIN Recycling (0 to 15)

When active, the instrument will check when a new PIN is set that it has
not been used within the last number of specified changes. This stops a
user from switching between frequently used PINs.

• User Lockout (0 to 10)

If selected, the instrument will automatically lockout a user if they enter

their PIN incorrectly more than the specified number of times.

An administrator will then be required to reset or change that user’s PIN.

The default administrator profile (Page 51) cannot be locked out.

• Fast PIN Change (no / yes)

This will allow the user to change their PIN without having Setup rights.
These are required to gain access to the Users menu where PINs are
usually changed.

A fast PIN change can be performed from the login screen. Whilst logging
in a user should enter their username or their PIN. When entered press
the Change button.

If the user enters their login details correctly a new PIN will be requested.
The new PIN must be input twice to ensure no errors are made.

After the PIN has changed, the instrument will continue to whatever was
requested that required a login to access.

Page 48 37-228’03 Rev. A January 2019 ADP450

 User Types
User Types define the Access Rights that each user can have. There are
three default User Types in the instrument.

User Type administrator calibrator operator

Read Yes Yes Yes
Mode / Method
Yes Yes Yes
Selection

Access Rights
Method Editing Yes Yes No
Zero Yes Yes Yes
Span (Top Calibration) Yes Yes No
Data Yes Yes No
Setup Yes No No
Maintenance Yes No No
Approval Level Approver Reviewer Submitter

When creating a new User a User Type is assigned. This gives each user
an associated meaning when electronically signing results and the
required access rights to appropriate menus.

Along with the three default user types, new user types can be created.

To define a user type, select the Settings option in the Users Menu, then
select:

• 2. User Types
• 1. Add new…

First give a title for the user type e.g. technician.

Then the access rights for that user type can be set, these rights give
access to the following:

Right User access

Allows the user to take readings. This feature is only

Read available when User Traceability is set to login, see
page 31.

If using the Mode Menu, users will be able to change

Mode / Method the measurement settings. If Methods are active,
Selection users will be able to change Method but Method
measurement settings cannot be changed.

Method Editing Allows the user to edit the settings of a Method.

The user will be able to perform Zero calibrations.
Zero This feature is only available when User Traceability
is set to login.
This will allow access to the Calibration menu. This
Span
should be restricted to users who are trusted to
(Top Calibration)
handle the expensive, traceable standards.

Gives access to Data in the Main Menu or via the

Data button from where logs can be reviewed and
Data
downloaded. PDF records can also be downloaded
from here.

ADP450 37-228’03 Rev. A January 2019 Page 49

 Right User access

With access to the Setup Menu, all of the

instrument’s settings can be changed along with
Setup
security settings. This should be restricted to the
highest level users.

This should be granted to users responsible for the

Maintenance
care of the instrument.

Sets the level at which a user can countersign saved

Approval Level
results, see below.

The user type will then be selectable when adding a new user. The user
type can be edited from the User Type Menu by selecting it again.

Approval Levels
The instrument has three approval levels – Approver, Reviewer and
Submitter – allowing users to countersign results and indicate their
acceptance, required in some regulatory environments.

To sign a result, select it in the Reading Log (Page 35) and press the
Signing button. This shows all previous signings.

Press the sign button, a login will be required to confirm the user’s
identity then select pass or fail to apply an electronic signature. An
additional comment can be added at this time.

If a higher Approval level User signs a result before others, lower level
signatures will not be required, e.g. if a User with Approver level signs a
result before any other user their signature will also be applied to the
Submitter and Reviewer level. If a Reviewer signs first, their signature will
also be applied to the Submitter level, leaving only an Approver’s
signature needed to be complete.

The signature of the user who performed the reading will still accompany
the result.

Each result summarises applied signatures with a traffic light system.

Grey indicates not yet signed, green signed as pass, red signed as failed.

Users without Data Rights and Submitter approval levels will be given
access to only their unsigned results to enable signing. Data transfer will
not be enabled.

Page 50 37-228’03 Rev. A January 2019 ADP450

 Users
After setting the Security Level and defining User Types as required,
Users can then be added (not available with the Basic protection level).
The current Users are shown in the Users menu.

• Default users

There are three default users, these can only have their PINs changed or
ID tags paired (see Page 53).

Username administrator calibrator operator

Default PIN 000 355 123
Signature Administrator Calibrator Operator
User type administrator calibrator operator

The default PINs will have the required number of leading zeroes
depending on the length setting, e.g.

PIN Length PIN Code

3 355
4 0355
8 00000355

The administrator PIN is initially set to all zeroes (000). This disables
all security features, giving all users unrestricted instrument
access. The administrator PIN must be changed before security
settings come into effect.

If the operator PIN is set to all zeroes (000), then there will be
unrestricted access to the Mode/Methods selection, ideal for product
familiarisation and training.

If the PIN type is set to Password then a default value will not be set. The
passwords for the default users will have to be set manually.

WARNING! Access to all the functions is only possible for the

administrator or a user with full rights after entering the correct PIN.
DO NOT FORGET IT!

In the event of the administrator PIN being forgotten, contact Bellingham

+ Stanley or your supplier for assistance.

• Adding a user

Select the Add new… option at the top of the Users Menu to add a new
user. Up to 47 additional users can be added, giving a total of 50 users
with the default profiles included.

First a username is requested. This is the name that will be used to login
when User Selection is set to user and PIN or password.

The user’s Signature is then required, this will be used for Traceability
purposes, for instance on calibration reports and when recording results
with User codes set to login (see Page 32).

The user’s PIN will then need to be set (entry is requested twice to avoid
errors). This will have to be input at the first login at CFR and High
security level.

Finally, select the User Type to set their access rights.

ADP450 37-228’03 Rev. A January 2019 Page 51

 From the User list, created user details can also be edited. Simply select
the user for whom details need to be changed. This will include
information about the status of the user account.

If protection level is set to high or CFR, users will not have the option to
change PINs from the Manage Users menu, however the user can have
their PIN reset. This will cause the instrument to request that the user
change their PIN when they next attempt to login.

• Disabling Users

At higher security levels Users cannot be deleted to prevent the reuse of

electronic signatures. Instead the accounts can be deactivated. Select
the User in the User List and select Option 4. Advanced then 2. Active.
Set this to “No” to disable the user, they will then not be able to login.

If a user is disabled due to multiple incorrect PIN entries (User Lockout in

use) the Active status will be set to “No”, change this to “Yes” to allow the
user to resume work.

Print User Lists

If two factors are required to login e.g. if “user and PIN” is active, when a
user tries to access a Menu, for example, the instrument will first request
their username.

Passwords will not be shown at the higher security levels.

Print User Types will give a list of User Rights associated with each User
Type.

Logging in/out
If two factors are required to login e.g. if “user and PIN” is active, when a
user tries to access a Menu, for example, the instrument will first request
their username.

If “remember last user” is active there may already be a username in the

text box.

After confirming username entry or if user selection is set to “PIN only”,

the PIN entry screen will appear.

After typing in the PIN and pressing OK, if both the username and PIN
were correct, the user will have access to the requested Menu (if they
have sufficient rights). The user’s username will scroll in the title bar.

Instead of typing out the user’s details login can be performed simply by
swiping an assigned RFID tag.

Page 52 37-228’03 Rev. A January 2019 ADP450

 A USB keyboard, available from Bellingham + Stanley will facilitate entry
when using “Password”

If the username or PIN was incorrect, or the user does not have sufficient
rights for the current activity, a notification will be given.

If “keep logged in” is set to 0 (off) users will be automatically logged out
when they return to the Measurement Display. However, if “keep logged
in” has a value, to log a user out hold down the left arrow key whilst on
the Measurement Display.

Logging out can also be performed by swiping an RFID tag.

RFID Tags
RFID (radio frequency identification) tags are used to wirelessly identify a
user. Several RFID tags come with the instrument and additional tags
can be obtained from Bellingham + Stanley, see the Accessories section.

ID tags are read by placing the tag within 2cm of the reader:

RFID tags are paired to a user. This enables the user to be identified and
logged in simply and quickly without the need for further authentication
(PIN or username entry).

As RFID tags alone will authenticate a user, ensure that ID tags are
protected from misuse.

To pair an ID tag with a user, first select the user from the Users Menu.

The Pair ID Tag value should be none. Select Pair ID Tag and the
instrument will request that the relevant ID tag is placed onto the reader.

ADP450 37-228’03 Rev. A January 2019 Page 53

 Reading setup
In addition to the measurement settings there are several other factors
that can affect how readings are taken.

To access the Reading menu, press Menu, then select:

• 3. Setup
• 3. Reading

Reading response
The instrument measurement is the result of a running average of a
number of measurements. The quantity of measurements the instrument
averages over affects the measurement response, stability, and reading
time.

The greater the averaging, the better the stability, the disadvantage is a
slower response to a change in sample, and longer reading time. The
instrument has 3 response settings.

Response Time Description

fast 2 seconds Ideal for low resolution.
medium 20 seconds Balances reading time and stability
Only necessary if displaying results to a
slow 40 seconds
high resolution

Adding extra scales

Scales can be added to convert the readings to other standard units, or
company specific units for a particular product.

To access the Scales menu, select option 2 (Scales) in the Reading

Menu. This will present a list of current custom and installed library scales
and the option to add new.

Page 54 37-228’03 Rev. A January 2019 ADP450

 Adding a library scale
Select the Add new… option in the Scales List screen, then select From
Library. The following scales can then be installed.

Library
Scale name Scale ID Description
number
Specific rotation, using a
1 specif. rotation sr
known concentration
Concentration, using a known
2 conc. (g/cm3) co
specific rotation
Purity, using a known Brix
3 purity pu
value
% Inversion of sucrose
4 % inv sucrose pi
solution
% Change of product over a
5 % change pc
period of time
Specific rotation, using a
6 specif. rotation-m sm known concentration and
moisture percentage

Once loaded, the scale will be available for use in Mode or Methods.

When a library scale is activated additional Advanced Measurement

Settings become available. Values can either be fixed to the value set in
the measurement settings or variable. When set to variable, if a
measurement is recorded (saved or printed) the instrument allows certain
values to be changed. Fixed or variable can be Altered by selecting the
scale again in the Scale List.

Library scales present results in special Measurement Display formats.

ADP450 37-228’03 Rev. A January 2019 Page 55

 Specific rotation
Specific rotation is calculated using the following formula:

α l20
[α ]l20 = c
×l
100

where:

[α ] is the specific rotation in angular degrees per dm (decimetre) and

20
λ
3
per g/cm , at 20°C and measured at a particular wavelength, λ

α λ20
is the optical rotation in angular degrees at 20°C and measured at a
particular wavelength, λ
3
c is the concentration in g/100cm

l is the length of the polarimeter tube in dm (decimetres) (automatically

converted from mm)

When the specific rotation scale is activated additional Advanced

measurement settings become available.

When activated, the Measurement Display will change to accommodate

the extra values associated with the specific rotation scale.

The concentration value can be set in the Advanced measurement

settings but users will also be prompted to confirm the value at the start
of each print.

Page 56 37-228’03 Rev. A January 2019 ADP450

 Concentration
Concentration uses a rearrangement of the specific rotation formula:

α l20
c=
[α ]l20 × l
where:
3
c is the concentration in g/cm

[α ] is the specific rotation in angular degrees per dm and per g/cm , at

20
λ
3

20°C and measured at a particular wavelength, λ

α λ20
is the optical rotation in angular degrees at 20°C and measured at a
particular wavelength, λ

l is the length of the polarimeter tube in dm (automatically converted

from mm)

When the concentration scale is activated additional Advanced

measurement settings become available.

When activated, the Measurement Display will change to accommodate

the extra values associated with the concentration scale.

The specific rotation value can be set in the Advanced measurement

settings but users will also be prompted to confirm the value at the start
of each print.

ADP450 37-228’03 Rev. A January 2019 Page 57

 % Inversion of sucrose solution
The instrument can automatically calculate % inversion of sucrose
solution using the following formula:

%inversion = (rsuc − oA / l / c *158.874) /(rsuc − rinv) *100

where:

rsuc is the rotation in °A of non-inverted 15 °Brix sucrose solution in

100mm tube

rinv is the rotation in °A of fully-inverted 15 °Brix sucrose solution in

100mm tube

o
A is the rotation in °A of the sucrose solution

c is the concentration of sucrose in g/l

l is the length of the polarimeter tube in dm (automatically converted

from mm)

When the % inversion scale is activated additional Advanced

measurement settings become available.

When activated, the Measurement Display will change to accommodate

the extra values associated with the inversion scale.

The concentration value can be set in the Advanced measurement

settings but users will also be prompted to confirm the value at the start
of each print.

Page 58 37-228’03 Rev. A January 2019 ADP450

 % Change of product over a period of time
The instrument can automatically calculate % change of product over a
period of time using the following formula:

%change =(oA * cstart / c − rstart ) /(rstart − rend ) *100

where:

o
A is the rotation in °A of the product sample

rstart is the rotation in °A of product sample in its start state (e.g. non-
inverted)

rend is the rotation in °A of product sample in its end state (e.g. fully
inverted) * cstart / cend

cstart concentration of product start state sample in g/l

cend concentration of product end state sample in g/l

c is the concentration of product sample in g/l

When the % change scale is activated additional Advanced measurement
settings become available.

When activated, the Measurement Display will change to accommodate

the extra values associated with the % change scale.

ADP450 37-228’03 Rev. A January 2019 Page 59

 Specific rotation with a known moisture content
Specific rotation is calculated using the following formula:

α l20
[α ]l20 = c 100 − m
× ×l
100 100

where:

[α ] is the specific rotation in angular degrees per dm (decimetre) and

20
λ
3
per g/cm , at 20°C and measured at a particular wavelength, λ

α λ20
is the optical rotation in angular degrees at 20°C and measured at a
particular wavelength, λ
3
c is the concentration in g/100cm

m is the moisture content percentage

l is the length of the polarimeter tube in dm (decimetres) (automatically

converted from mm)

When the specific rotation scale is activated additional Advanced

measurement settings become available.

When activated, the Measurement Display will change to accommodate

the extra values associated with the specific rotation scale.

The concentration and moisture percentage values can be set in the

Advanced measurement settings but users will also be prompted to
confirm the value at the start of each print.

Page 60 37-228’03 Rev. A January 2019 ADP450

 Purity
This instrument has the facility to automatically calculate the purity of
sugar solutions. The value of purity is derived from the angular rotation of
the sample (supplied by the polarimeter), the mass concentration (brix)
value (supplied by a refractometer, see below) and the density (derived
from the brix value).

As there are different methods used throughout industry for calculating

sugar purity, the instrument has a number of settings which allow the
instrument to be setup using the appropriate calculation.

The standard equation used to calculate sugar purity is:

reading × purityconst
purity =
density × brix

where:

reading is the polarimeter reading in °Z

purityconst is a user definable purity constant

density is calculated as a function of brix in accordance with the

ICUMSA Specification and Standard SPS-4 (1994)

brix is the Brix value of the sample

When the purity scale is activated an additional Advanced measurement
setting becomes available.

When activated, the Measurement Display will change to accommodate

the extra values associated with the purity scale.

The Brix value can be set in the Advanced measurement settings but
users will also be prompted to confirm the value at the start of each print.

The purity constant can be changed from the scale list in the Reading
section of the Setup Menu. Select the purity scale and then select
constant. The desired constant can then be entered. The default purity
constant is 28600.

ADP450 37-228’03 Rev. A January 2019 Page 61

 Advanced Purity Settings
As well as manual input, the polarimeter can receive the °Brix value for a
sample from the RFM700 and RFM300 series of refractometers. The
instruments can be interfaced by Network or Serial cable, depending on
the generation of refractometer available.

Open the Main Menu, then select:

• 3. Setup
• 3. Reading
• 2. Scales
• 2. Purity

Editing the Purity equation

Select equation in the Purity Settings Menu to choose the equation used
to calculate purity.

• Standard

The default purity equation, as discussed above, will be used.

• Equation A

Purity will be calculated using the following equation:

𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓 × 𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟
𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝 = × 100
𝑏𝑏𝑏𝑏𝑏𝑏𝑏𝑏
Where 𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓 is a function of the specific gravity (20°C/20°C) of
sucrose:

26.00
𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓 =
99.718 × 𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠 𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔

The 𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠 𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔 is calculated from the Brix value.

𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟 is the polarimeter reading in °Z

𝑏𝑏𝑏𝑏𝑏𝑏𝑏𝑏 is the Brix value of the sample

Page 62 37-228’03 Rev. A January 2019 ADP450

 • Equation B

Purity will be calculated using the following equation:

𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟
𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝𝑝 = × 100
𝑏𝑏𝑏𝑏𝑏𝑏𝑏𝑏
𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟𝑟 is the polarimeter reading in °Z

𝑏𝑏𝑏𝑏𝑏𝑏𝑏𝑏 is the Brix value of the sample

• User defined:

A user defined equation for purity can be created using the following
elements:

!degreez polarimeter reading in °Z

!degreea polarimeter reading in °A
!temp polarimeter temperature in °C
!brix °Brix by refractometer
!purityconst Purity scale constant
!density density derived from Brix value according to ICUMSA SPS-4

Standard mathematical operators (e.g. +,-,/,*) can be used. Type any

required constants as numbers. When inputting an equation ensure
spaces are left between elements.

For example, purity equation B would appear as:

Connection to an RFM
All required cables are available from Bellingham + Stanley, see the
Accessories section. To set the connection type, select Refractometer in
the Purity Settings Menu.

• Manual

The Brix value can be set in the Advanced measurement settings but
users will also be prompted to confirm the value at the start of each print.

• Serial

The print mode of the attached RFM must be set to CSV serial using:
Baud rate: 9600 Word length: 8 bit, no parity

Refer to the RFM’s user guide for instructions.

Depending on the generation of instruments a FTDI USB to RS232

converter may be needed.

ADP450 37-228’03 Rev. A January 2019 Page 63

 The ADP will instruct for all USB devices to be removed. Push ok.
Insert the USB/Serial cable that is connected to the RFM. Push ok. The
instruments will now be connected.

If a serial printer was previously connected via a USB hub, plug it back
into its original port.

• Network

The print mode of the attached RFM must be set to CSV network, refer to
the RFM’s user guide for instructions.

Select the Network option on the ADP. An IP address will be requested.

This can be either:

The IP address of an RFM located somewhere on a LAN (both

instruments must be on the LAN).

Or:

The IP address of an RFM connected directly to the ADP via a cross-over

Ethernet cable.

The RFM’s IP address can generally be viewed in the Information section

of its Help Menu.

Input the RFM’s IP address into the ADP and push ok. The instruments
will now be connected.

• CSV delimiter

If the CSV output of the refractometer does not use a comma to delimit
fields, the option to use a semicolon, space or tab delimiter is available
here.

• CSV Brix position

This sets the column of CSV output of the refractometer that the °Brix
value appears in e.g. from an RFM300-T with all traceability active:

03/05/18, 14:01:52, qx157, j.smith, 9.33, 20.0, 90, brix, none

The Brix value, 9.33, is in the fifth column, therefore set this value to 5.

• Connection test

On selection the instrument will wait to receive a CSV string. It will then
break it down into its component parts (ensure the delimiter is set
correctly first).

e.g.: 03/05/18, 14:01:52, qx157, j.smith, 9.33, 20.0, 90, brix, none

Page 64 37-228’03 Rev. A January 2019 ADP450

 Select the value which shows the °Brix value from the refractometer.
The CSV Brix position will then be automatically set.

Using these tools it may be possible to use non-RFM refractometers if a

suitable CSV output is available.

Once the RFM is connected and the CSV Brix position set, return to the
Measurement Display and ensure the purity scale is active and a reading
displayed.

Take a reading on the connected RFM and then press its print button.
The Brix value on the ADP’s display will be updated after a few moments.

Adding a custom scale

As well as the library scales, readings can be converted to any custom
units. To set up a custom scale, select Add new... on the Scales list then
select From Data.

Readings can be converted to custom units using the following formula:

2 3 4 5
reading = A + Bx + Cx + Dx + Ex + Fx

where: x = measured rotation (°a) - Offset

A,B,C,D,E,F are polynomial constants

• Enter the new scale name, (16 characters) e.g. coffee solids
• Enter the scale ID, (2 characters) e.g. cs
• Enter the 6 constants A to F. Any constants not required, (e.g. E
& F with a 4 term polynomial), should be set to 0.
• Enter Offset (this can generally be left as 0).
• Select the Number Format required for displaying and printing
the reading.

The scale will then be added to the Scales List and can subsequently be
Altered, Deleted or transferred to USB if a removable storage device is
present by selecting it again. The scale will also be available in
measurement settings.

Adding a custom temperature compensation

Temperature compensations can be entered in a similar manner to
scales via the Temp. Comp. menu (option 3 in the Reading Menu). This
will present a list of current custom temperature compensations and the
option to add new. To set up a custom temperature compensation, select
the Add new... option and enter data as discussed above. Offset and
resolution are not required for temperature compensations.

Temperature compensations are calculated using the following equation:

Compensated Reading = Uncorrected Reading

1+(Constant x (t – 20))

where: t = sample temperature (°C)

ADP450 37-228’03 Rev. A January 2019 Page 65

 USB and network scales
Custom scales and temperature compensations can also be transferred
from and to the instrument using the USB or network ports. This is useful
for reinstalling mistakenly altered scales or quickly transferring them
between instruments.

• USB

Place a USB removable storage device into the instrument then access
the Scales List or Temp. Comp. menu. Select the item that is required for
export then Export to USB. After giving the file an appropriate name it will
be saved to the USB device in a folder named either “Scales” or “TCs”.

The file can then be imported into another instrument or reloaded to the
same instrument in case of accidental loss. With the USB device in the
instrument select the Add New… option in either Scales List or Temp.
Comps. The From USB option will then be available. Select it then
choose the file to be imported.

The instrument checks the USB storage device for files in folders called
“Scales” for compatible scale files (extension .usc) or “TCs” for
temperature compensation files (extension .utc). By creating the required
folder and placing compatible files within, it is possible to import files
saved or created by other means

• Network

It is possible to import scales and temperature compensations to the

instrument via a network. Connect to the instrument via FTP (see Page
38), create a folder called “Scales” or “TCs” and place a compatible file
(.usc or .utc respectively) inside. Select the Add New… option in either
the Scales or Temp. Comp. Menu and then From Network. A list of
compatible files in the folder will be displayed, simply select the one
required for it to be installed. The file will be removed from the network
folder once installed.

Page 66 37-228’03 Rev. A January 2019 ADP450

 System settings
To make changes to the system settings press Menu, then select:

• 3. Setup
• 5. System

Language
There are several language options available in the instrument by default.

Select the required language and press Yes to confirm the change.

If the language you require is not available, contact Bellingham + Stanley

and we will do our best to accommodate your needs.

Time/Date

• Date Format

The instrument can display the date in two formats, “dd/mm/yy” and
“mm/dd/yy”. These formats will also be used on any printouts.

• Summer Time

This will adjust the instrument’s clock for daylight saving time (adding one
hour to the clock).

Set it to yes to advance the clock one hour. Set summer time to no to
revert to the system time

• Set Clock…

Enter the time and date in the format displayed on screen, as specified in
Date format.

Use the arrow buttons to change the cursor position.

Press ok when the clock is set to the correct time and date.

• Time Server

Time Server allows the instrument’s clock to be synchronised to a local

server if connected to a LAN.

First use Set Clock... to ensure the time is approximately correct. Select
Time Server and type in the IP address or name of a time server on the
LAN. Confirm entry and the instrument will attempt to connect to the
server.

The clock will resynchronise at switch on and midnight.

• UTC Offset

This allows for server set time to be adjusted to a local time zone. Input a
value in hours to add or subtract from the synchronised time.

ADP450 37-228’03 Rev. A January 2019 Page 67

 Display

• Display Brightness

If the display is difficult to read its brightness can be altered to be suitable

for the operating environment.

• Remote Display

By turning Remote Display on it will be possible to interact with the

instrument using PC software available from Bellingham + Stanley
website.

The instrument and PC must be connected to a Local Area Network to

use the remote display feature.

• Setup

The Header, Temperature, OD, and Configuration can be individually

selected to be displayed. If these four options are hidden then the screen
will show only the reading and the softkey functions.

The softkey option allows the colouring of the softkeys to be changed.

Grey

Solid

Corner

Top

Temperature control
Under certain conditions it may be useful to disable the temperature
control, this can be done by setting this option to off.

Clone settings
Clone is ideal for restoring parameters if a default has been performed.

The instrument settings can be backed up from the Maintenance Menu,

see Page 71. With the USB removable storage device that contains a
valid Clone file inserted into the instrument, enter the System Menu and
the Clone option will be available.

This will copy all settings from the Clone file to the current instrument,
useful if settings are accidentally changed or lost, or if multiple
instruments are in use only one needs to be setup then the settings

Page 68 37-228’03 Rev. A January 2019 ADP450

 copied via USB to all other instruments. This enables quick integration of
new or hire instruments.

Once the Clone option is accessed and confirmation given a list of

instruments from which backups are available will be displayed.

Select the instrument, by serial number, which has the settings required
for the current instrument.

A list of date and time stamped backups will then appear. Select the
backup with the required settings.

A backup of the current settings will be made in case the Clone needs to
be undone, this will be labelled as an “autobackup”. The selected backup
will then be installed.

If more than one USB removable storage device is detected the

Clone will not be performed.

Do not remove the USB removable storage device during a Backup

or Clone as this can lead to loss of data.

ADP450 37-228’03 Rev. A January 2019 Page 69

 Section 4: System
maintenance
Maintenance Menu ................................................................................ 71
Maintenance Report ................................................................... 71
Backup ....................................................................................... 71
Software update ......................................................................... 71
Information and Help ............................................................................ 72
Setting default values ........................................................................... 72

Page 70 37-228’03 Rev. A January 2019 ADP450

 Maintenance Menu
There are several options to help keep the instrument in good working
order. To access the Maintenance Menu select option 4 in the Main
Menu.

Maintenance Report
It is recommended that the instrument is regularly serviced by Bellingham
+ Stanley trained personnel. Contact Bellingham + Stanley or your local
distributor to arrange a service visit.

The Maintenance Report provides a useful reminder of when servicing is

required. The reminders also appear on the instrument’s standby screen.

A coloured ribbon symbol indicates that the instrument was last serviced
by Bellingham + Stanley personnel or an engineer who has received
training from Bellingham + Stanley.

Backup
The Backup option creates a copy of all the settings changed in the
instrument. Using Clone (see Page 68) these changes can be reloaded at
a later date to undo accidental changes or deletions.

Alternatively, the settings can be Cloned to another instrument. This is

useful if multiple ADP450s are available for use. The required settings
only need to be configured on one instrument then they can be easily
Cloned to all the others. This will also enable quick integration of any new
or hire instruments.

To create a backup insert a USB removable storage device before

entering Backup in the Maintenance Menu. Select Backup to USB, option
1, and the instrument will create a backup file with a date and time stamp.

Backup & Clone is ideal for restoring parameters if a default has been
performed. Make backup part of your SOP.

A reminder can be set in the Backup section of the Maintenance Menu.

When the warning period expires, on the next attempt to take a reading
or print, a reminder will appear on screen. Press ignore to delay the
procedure for a brief period.

Software update
Bellingham + Stanley pursue a policy of continuous product development
and improvement and as such, from time to time, new versions of
software will become available. Contact Bellingham + Stanley to obtain
new software.
ADP450 37-228’03 Rev. A January 2019 Page 71
 When new software is received it can be installed using a USB
removable storage device. Place the software update folder on the USB
device, ensure no other USB removable storage devices are in the
instrument, and then insert the device holding the update files. Access
the Maintenance Menu and select option 3, Update Software.

Confirm the software installation and the instrument will then perform the
update.

The instrument will restart after the update is complete.

Do not remove the USB removable storage device during the update
process as this can lead to loss of data.

Information and Help

From the Main Menu the Help Menu can be accessed (option 5).

From here information regarding the instrument’s software, hardware and

network configuration can be seen and printed. This is useful to have
available when contacting Bellingham + Stanley for assistance and can
be printed to the selected printer. Contact details of Bellingham + Stanley
are provided in the Help Menu.

Copies of this User Guide and compatible PC software can also be made
to a USB removable storage device.

Setting default values

If the instrument fails to start up correctly after switching on, or it is
functioning unexpectedly, it could be advisable to reset certain settings to
the original factory set (or default) values.

Unplug the instrument. Power up the instrument and wait for the loading
screen to appear. Press and hold down the Green softkey (power),
‘Default Request’ will appear at the bottom of the screen.

Do not release the button until the restore / default message appears.

Page 72 37-228’03 Rev. A January 2019 ADP450

 Press Restore to load a backup stored on a USB removable storage
device. The instrument will request a USB device is inserted then the
Clone procedure can be carried out (Page 68).

Press Default to return various settings to their factory configuration.

Default should be performed as a last resort as all customisation

can be lost. Contact Bellingham + Stanley for assistance.

Initially the options will be set to “no”. Select the settings that you want to
return to default values by setting them to show “yes”.

When set as desired press the Quit button.

The instrument will ask the user to confirm their choice. Pressing Yes will
carry out the default.

ADP450 37-228’03 Rev. A January 2019 Page 73

 Section 5: Specification
Specification .......................................................................................... 75
Performance ............................................................................... 75
Optical path ................................................................................ 75
Temperature ............................................................................... 75
Temperature compensation ....................................................... 75
Light source ................................................................................ 75
Communication ports ................................................................. 75
Physical ...................................................................................... 76
Power requirements ................................................................... 76

Page 74 37-228’03 Rev. A January 2019 ADP450

 Specification

Performance

Scale ºA - Angular °Z – ISS

-89 to +89
Range -225 to +225
(±355 Method selectable)
Resolution 0.1 / 0.01 / 0.001

Accuracy (±) 0.01 0.03

Reproducibility 0.002 0.005

Optical Density 0.0 to 3.0

Optical path
Tube length 10-200 mm
Tube diameter 3-8 mm

Temperature
Ambient operating range 15 to 35 ºC
Storage -5 to 60 ºC
Control range 20°C (min.) (not <7.5°C below ambient) to 30°C (max.)
Stability ±0.2°C
Sensor Accuracy ±0.1°C

Temperature compensation
None -
Sugar 5 to 40 ºC
Quartz 5 to 40 ºC
User defined -

Light source
Light emitting diode with interference filter 589 nm
Beam diameter, maximum 4 mm

Communication ports
Type A x1
USB
Type B x1
Ethernet 10/100Mbps
Serial via FTDI USB to RS232 converter

ADP450 37-228’03 Rev. A January 2019 Page 75

 Physical
Length (polarimeter module only) 64 cm
Width (polarimeter module only) 34 cm
Height (polarimeter module only) 15 cm
Weight (polarimeter module only) 9 kg

Power requirements
Voltage 100 to 240 V
Frequency 50 to 60 Hz
Current <2A

Page 76 37-228’03 Rev. A January 2019 ADP450

 Section 6: Accessories and
Contact
Accessories ........................................................................................... 78
Quartz Control Plates ................................................................. 78
Printers ....................................................................................... 78
Other accessories ...................................................................... 78
Interconnecting cables ............................................................... 79
Spares ........................................................................................ 79
XPC Polarimeter tubes ............................................................... 79
Low Volume Polarimeter Tubes ................................................. 79
XPC Temperature ...................................................................... 79
Upper lids ................................................................................... 80
Power supplies ........................................................................... 80
Contact ................................................................................................... 81
UK / International........................................................................ 81
United States of America ........................................................... 81
Germany ..................................................................................... 81
France et DOM-TOM.................................................................. 81
About Xylem ............................................................................... 81

ADP450 37-228’03 Rev. A January 2019 Page 77

 Accessories

Quartz Control Plates

Bellingham + Stanley, a UKAS accredited Calibration Laboratory number
0834, offer a choice of Quartz Control Plates (QCP) for verifying and
calibrating polarimeters. QCPs are made to the highest standard and
may be supplied with an optional Certificate of Calibration, showing
traceability to PTB.

Nominal Value
Type 100 °Z 15 °Z -30 °Z Code
(34.6 °A) (5.2 °A) (-10.4 °A)
Quartz Control
34-30
Plate: Set A
Quartz Control
34-31
Plate: Set B
Quartz Control
34-32
Plate: Set C
Quartz Control
34-39
Plate: Set Full

As part of Good Laboratory Practice, it is recommended that Quartz

Control Plates are sent to our UKAS laboratory at regular intervals
determined by the user’s own Standard Operating Procedure for
inspection and recertification.

Code
QCP UKAS certificate of calibration traceable to PTB (589nm) 90-803
QCP UKAS certificate of calibration traceable to PTB (546-
90-805
900nm (each))
QCP manufacturers certificate of calibration (300-545nm) 90-807
Quartz Plate thermal block assembly 34-241
Quartz Control Plate nominal 100°Z (34.6°Angular) 34-20
Quartz Control Plate nominal 15°Z (5.2°Angular) 34-21
Quartz Control Plate nominal -30°Z (-10.4°Angular) 34-22
XPC Adaptor for QCP 35-98

Printers
Code
Dot matrix impact printer serial: Euro version 230V 55-14
Dot matrix impact printer serial: UK version 230V 55-15
Dot matrix impact printer serial: US version 110V 55-16
USB Printer – Thermal: 110-240V 55-18

Other accessories
Code
Barcode scanner - USB version 55-82
USB keyboard 55-86

Page 78 37-228’03 Rev. A January 2019 ADP450

 Interconnecting cables
Code
Computer (9way D type) 54-07
LAN cable – 2m 54-075
USB cable A to B (male/male) – 2m 54-081
USB cable A to A (male/male) – 2m 54-082
USB Serial Adaptor 55-85
USB Hub 55-88

Spares
Code
Paper for dot matrix impact printers (20) 55-91
Printer ribbon for dot matrix impact printers 55-93
Paper for USB thermal printer (20) 55-95
ID tag card (single) 37-530
ID tag key-fob pack (3) 22-071
ID tag key-fob pack (10) 22-072

XPC Polarimeter tubes

Code
Stainless steel tube, 2 filler, 100mm 35-90
Stainless steel tube, 2 filler, 200mm 35-91
Stainless steel tube, funnel filler, 100mm 35-92
Stainless steel tube, funnel filler, 200mm 35-93
Temperature probe sleeve 37-565
Spare cover glasses (6) 37-540
Spare gaskets (10) 37-541

Low Volume Polarimeter Tubes

Length Volume
Type * Code shown Code
(mm) (ml)
5 0.1 35-74
Low volume
10 0.2 35-73
continuous flow-
25 0.5 35-72
through tube
50 1.0 35-71*
Compatible with slotted upper lid 37-010

Code
Low volume cover glass (6) 35-80
Low volume spare gaskets, Nitrile (10) 35-81
Low volume spare gaskets, Viton™ (2) 35-85
Low volume spare gaskets, Kalrez® (2) 35-87

XPC Temperature
Code
XPC-100 Adaptor for 100mm stainless steel tube 35-95
XPC-200 Adaptor for 200mm stainless steel tube 35-96
XPC Adaptor for low volume stainless steel tubes 35-97
XPC Adaptor for QCP 35-98

ADP450 37-228’03 Rev. A January 2019 Page 79

 Upper lids

Type Code.

No slots 37-008

2 slots 37-009

2 slots 37-010

4 slots 37-011

4 slots 37-012

Power supplies
Code
Power supply (unsealed) 110-230V 55-105
Mains lead for 55-106 with plug suitable for:
Switzerland 61-181
Denmark 61-182
India / South Africa 61-188
Australia 61-189
UK 13 Amp square pin to BS1363/A 61-191
United States (3 pin) 61-192
Europe (Schuko) 61-193

Page 80 37-228’03 Rev. A January 2019 ADP450

 Contact
For product support, please contact your local supplier or contact us via
our regional offices below:

UK / International United States of America

sales.bs.uk@xyleminc.com sales.bs.us@xyleminc.com

Bellingham + Stanley Ltd. Bellingham + Stanley

Longfield Road 90 Horizon Drive
Tunbridge Wells Suwanee
Kent TN2 3EY GA 30024
United Kingdom USA
Tel: +44 1892 500400 Tel: 678 804 5730
Fax: +44 1892 543115 Fax: 678 804 5729
Toll free (USA only): 800 678 8573

Germany France et DOM-TOM

France Métropolitaine ainsi que les
Départements et Territoires d’Outre-Mer
bs.germany@xyleminc.com

Xylem Analytics Germany analytics.info-fr@xyleminc.com

Dept. Bellingham + Stanley
Hattenbergstr. 10 Xylem Analytics France
55122 Mainz Dept. Bellingham + Stanley
29, rue du Port
Tel: +49 (0) 6131 66 5111
92022 NANTERRE
Fax: +49 (0) 6131 66 5001
Tel: +33 (0)1 46 95 33 56
http://www.bellinghamandstanley.com

About Xylem
Xylem |ˈzīləm|

1) The tissue in plants that brings water upward from the roots;
2) a leading global water technology company.

We’re a global team unified in a common purpose: creating advanced

technology solutions to the world’s water challenges. Developing new
technologies that will improve the way water is used, conserved, and re-
used in the future is central to our work. Our products and services move,
treat, analyze, monitor and return water to the environment, in public
utility, industrial, residential and commercial building services, and
agricultural settings. With its October 2016 acquisition of Sensus, Xylem
added smart metering, network technologies and advanced data
analytics for water, gas and electric utilities to its portfolio of solutions. In
more than 150 countries, we have strong, long-standing relationships
with customers who know us for our powerful combination of leading
product brands and applications expertise with a strong focus on
developing comprehensive, sustainable solutions.

To learn more about Xylem products and services, including other

analytical devices, please visit our websites:

http://www.xylemanalytics.com

http://www.xyleminc.com

ADP450 37-228’03 Rev. A January 2019 Page 81