Name: Date: Marks /8
AS Biology Practice Worksheet
Topic: 2.1 Testing for Biological Molecules
P1 Multiple Choice Questions
1 A student was asked to estimate the concentration of glucose in a solution using the
Benedict’s test. The student was provided with a 1.0 mol dm glucose solution and was
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told to make a 0.6 mol dm solution by proportional dilution.
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Which row shows the correct volumes of both 1.0 mol dm glucose solution and distilled
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water needed to make the 0.6 mol dm solution?
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2 A sample of a solution was tested for reducing sugar and the result was negative.
Another sample of the same solution was then tested for non-reducing sugar and the
result was positive.
Which step in the test for non-reducing sugar breaks the glycosidic bonds?
A addition of Benedict’s reagent
B addition of sodium hydroxide
C boiling with hydrochloric acid
D heating to 80 °C
3 After boiling a sample of milk with Benedict’s solution, a yellow colour is observed.
Which conclusion about the sample of milk is correct?
A Reducing sugars are not present.
B Reducing sugars are present.
C There is a high concentration of fructose.
D There is a low concentration of sucrose.
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4 A student carried out four tests for biological molecules on a solution. The results are
shown in the table.
Which three molecules may be present in this solution?
A glucose, starch, globin
B globin, glucose, collagen
C starch, sucrose, collagen
D sucrose, globin, collagen
5 A student carried out the Benedict’s test on a sample and got a negative result.
What should the student do to confirm there are no sugars present in the sample?
A boil the sample for 5 minutes then repeat the Benedict’s test
B boil with alkali, neutralise with hydrochloric acid and repeat the Benedict’s test
C boil with hydrochloric acid, neutralise with alkali and repeat the Benedict’s test
D repeat the Benedict’s test but add more Benedict’s reagent
6 Diastase is an enzyme that breaks down starch into maltose. Maltose is a reducing sugar.
A sample of starch is treated with boiled diastase and left for 15 minutes. Samples of the
mixture are then tested with iodine solution and with Benedict’s reagent.
What are the results?
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7 A student carries out a semi-quantitative test with Benedict’s solution.
Which statement about this procedure is correct?
A It detects only the presence or absence of glucose.
B It provides an indication of relative reducing sugar concentrations.
C The precipitate needs to be filtered, dried and weighed to give the reducing sugar
concentration.
D A colorimeter needs to be used to determine the glucose concentration.
8 A student carried out four tests on a sample of biological molecules.
Which conclusions made by the student are correct?
1. Fat was present.
2. Glucose was present.
3. Protein was present.
4. Starch was not present.
A 1, 2, 3 and 4
B 1, 2 and 4 only
C 1, 3 and 4 only
D 2 and 3 only
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