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B.Sc. Semester- I (Bio Group)
BOT-5IP-102 Botany Practical-I
Syllabus
Cell and Cell Organelles
• Study of electron microphotographs of prokaryotic and eukaryotic cel.
Study of electron microphotographs of virus, bacteria and eukaryotic cells for comparative
study of cellular organization.
Study of cell structure in Onion, Hydrilla and Spirogyra.
• Study of plastid for pigment distribution in Lycopersicon, Cassia and Capsicum.
Cell Division and Chromosomes
Study of permanent slides/photographs of different stages of miwsis meiosis, sex
chromosomes, polytene chromosome and salivary gland chromosomes.
Study of different stages of mitosis and meiosis in root-tip cells and flower buds
respectively of onion.
• Calculate the mitotic index of onion root tip cells.
• Study of induced aberrations in onion root tips employing chemicals and plant extracts.
Algae and Lichen
Algae- Study of morphology and anatomy of Nostoc, Volvox, Chara, Vaucheria,
Ectocarpus and Polysiphonia (vegetative and reproductive structures) by preparing
temporary slides and studying permanent slides.
Lichens: Study of growth forms of lichens (crustose, foliose and fruticose)
Fungi and Bryophyta
Fungi- Microscopic observation of vegetative and reproductive structures of Rhizopus,
Peziza and Puccinia through preparation of temporary slides and permanent slides.
Bryophytes- Study of morphology, anatomy, vegetative and reproductive organs of
Marchantia, Anthoceros and Funaria by preparing temporary slides and studying
permanent slides.
Study of enowned Indian scientists in the fields of phycology (M.0.P Iyengar), mycology
(K. C. Mehta), bryology (S.R.Kashyap) and lichens (D.D.Awasthi).
• Make a list of national and international institutes of repute in the fields of cytology,
phycology, mycology, bryology and lichens.
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B.Sc. Semester- II(Bio Group)
BOT-52P-104-Botany Practical-II
Syllabus
Practicals related to DNA
Isolation of Genomic DNA from Onio/Banana/Pineapple/etc.
Demonstration of Gel-electrophoresis
Practicals related to Genetics
To solve genetic problems based upon Mendel's laws of inheritance: Monohybrid
cross, Dihybrid cross, Back cross and test cross.
Induction of polyploidy using colchicines
Emasculation, Bagging and Tagging
Pteridophytes
Study of vegetative and reproductive stages of Selaginella, Equisetum and
Marsilea by preparing temporary slides and studying permanent slides.
• Study of fossil plant: Rhynia
Gymnosperms
Stucy of Vegetative and reproductive stages of Cycas, Pinus and Ephedra by preparing
temporary slides and studying permanent slides.
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