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Complement Fixation Test - Revised

The document outlines the steps of a complement fixation test used to detect the presence of antibodies, such as in the Wassermann Test for syphilis. It involves inactivating natural complement, mixing patient serum with known antigen, adding guinea pig complement, and then introducing sheep RBCs coated with hemolysin to observe for hemolysis. The results indicate the presence or absence of antibodies based on whether hemolysis occurs or not.

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7 views1 page

Complement Fixation Test - Revised

The document outlines the steps of a complement fixation test used to detect the presence of antibodies, such as in the Wassermann Test for syphilis. It involves inactivating natural complement, mixing patient serum with known antigen, adding guinea pig complement, and then introducing sheep RBCs coated with hemolysin to observe for hemolysis. The results indicate the presence or absence of antibodies based on whether hemolysis occurs or not.

Uploaded by

themasterof400
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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COMPLEMENT

FIXATION TEST
DEMONSTRATE PRESENCE OF ANTIBODY
(E.g. Wassermann Test - Detection of Syphilis Antibody)

STEP 1:
Natural complement inactivated
at 56°C for 30 minutes.
Patient’s serum is mixed with
known antigen. The mixture is
incubated.

STEP 2:
Complement (from guinea pig) is
added to the mixture and
incubated. This allows for any
antibodies in the patient’s serum
to bind to antigen and fix
(consume) the complement.

STEP 3:
After incubation, sheep RBCs
coated with hemolysin (anti-sheep
RBC antibodies) are added to the
mixture. The tube is incubated
again and observed for hemolysis.

STEP 4:

-OR-

Hemolysis

If no hemolysis occurs,
If hemolysis occurs, the
complement was used up
complement was not fixed in
earlier by the Ag-Ab complex in
step 2 – the result is negative
step 2 — result is positive
(no antibodies present in the
(antibodies are present in the
patient's serum)
patient's serum)

Known Antigen RBC w/ Hemolysin

Complement Unknown Antibody

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