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Job Aid 5.5-05-03 AFB Grading and Reporting

The document outlines job aids for reporting AFB results using Ziehl-Neelsen and FM staining techniques, detailing the number of AFB seen and corresponding reporting scales. It provides specific criteria for negative results, as well as various positive classifications based on the quantity of bacilli observed. This document is intended for internal use at Bedele General Hospital Laboratory, effective from January 1, 2020.

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158 views1 page

Job Aid 5.5-05-03 AFB Grading and Reporting

The document outlines job aids for reporting AFB results using Ziehl-Neelsen and FM staining techniques, detailing the number of AFB seen and corresponding reporting scales. It provides specific criteria for negative results, as well as various positive classifications based on the quantity of bacilli observed. This document is intended for internal use at Bedele General Hospital Laboratory, effective from January 1, 2020.

Uploaded by

blessbe45
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Bedele General Hospital Laboratory Document No: BGHL/BACT/ JA5.

5-05-03
Version No: 01
Job aids for AFB result reporting Page 1 of 1 Effective date: Jan. 01, 2020 GC

AFB reporting by Ziehl-Neelsen staining technique AFB reporting by FM staining technique


Number of AFB Seen (100x) Objective Reporting scale Number of Bacilli seen (20x) Objective Reporting scale
 No AFB found in at least 100 fields Negative  No AFB in one length No AFB observed
 1-9 AFB / 100 fields Exact figure  1-4AFB in one length Need confirmation
 10-99 AFB /100 fields 1+  5-49 AFB in one length Scanty
 1-10 AFB/ field (count at least 50 fields) 2+  3-24 AFB per field in at least 15 fields (1+)
 More than 10 AFB field (count at least 20 fields) 3+  25-250AFB per field in at least 6 fields (2+)
 >250 AFB per field (3+)

Skin slit smear reporting by Ziehl-Neelsen staining technique AFB reporting by FM staining technique
Number of AFB Seen (100x) Objective Reporting scale Number of Bacilli seen (40x) Objective Reporting scale
 0 Bacilli in 100 fields Negative:  No AFB in one length No AFB observed
 1-10 Bacilli in 100 fields 1+  1-2AFB in one length Need confirmation
 1-10 Bacilli in 10 fields 2+  3-24AFB in one length Scanty
 1-10 Bacilli ,on average, in each field 3+  1-6 AFB per field in at least 20 fields (1+)
 0-100 Bacilli ,on average, in each field 4+  7-60 AFB per field in at least 8 fields (2+)
 100-1000 Bacilli ,on average, in each field 5+  >60 AFB per field (3+)
 >1000 Bacilli ,on average, in each field 6+

Authority of Issue: BGHL Authorized Date: Jan 01, 2020.


This a controlled document for internal use only

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