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Discussion Practical 3 Microbe

The document describes the morphological, biochemical, and cultural characteristics of 6 different bacteria samples labeled D through I. The 3 key characteristics that distinguish the samples are: 1. Gram staining results - ranging from gram negative bacilli to coccobacilli to cocci in clusters. 2. Biochemical test results on TSI, motility, indole, citrate, and other tests - with varying results across the 6 samples. 3. Appearance of colonies on SS and CLED media - with different color, size, and other features described for each sample.

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36 views2 pages

Discussion Practical 3 Microbe

The document describes the morphological, biochemical, and cultural characteristics of 6 different bacteria samples labeled D through I. The 3 key characteristics that distinguish the samples are: 1. Gram staining results - ranging from gram negative bacilli to coccobacilli to cocci in clusters. 2. Biochemical test results on TSI, motility, indole, citrate, and other tests - with varying results across the 6 samples. 3. Appearance of colonies on SS and CLED media - with different color, size, and other features described for each sample.

Uploaded by

domo- kun
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Discussion

2. different characteristics
Morphology
D E F G H I
Gram Gram Gram Gram Gram Gram Gram
staining negative negative negative negative negative negative
bacilli coccobacilli coccobacilli bacilli in cocci in coccobacilli
cluster cluster in chain
Haemolysis Gamma Gamma Gamma Gamma Gamma Gamma
Size Medium Medium Medium Medium Medium Medium
Colour Yellowish Grey Grey Grey Yellowish Grey
Density Opaque Translucent Translucent Translucent Opaque Translucent
Elevation Flat Flat Convex Raised Raised Raised
Odour Fishy Fishy Fishy Fishy Fishy Fishy
Margin Entire Entire Entire Entire Entire Entire
Texture Mucoid Mucoid Mucoid Soft Soft Soft
Catalase Positive Negative Positive
Oxidase Negative Negative Negative

Biochemical tests
Test D E F G H I
TSI A/A with A/A without A/A with A/A with A/K A/A without
H2S gas H2S, gas H2S without gas
gas
Motility Motile Non motile Motile Motile Non motile Non motile
Indole Negative Positive Negative Positive Positive Negative
Citrate Negative Negative Positive Negative Negative Positive
PD Positive Negative Negative Negative Negative Negative
Urea Positive Negative Negative Negative Positive Negative
MR Negative Negative Negative Negative Negative Negative
VP Positive Positive Positive Positive Positive Positive
OF Ferment Ferment Ferment Ferment Ferment Ferment
(glucose)
OF Ferment Ferment Non Non Ferment Ferment
(sucrose) ferment ferment
MacConkey Non Non lactose Non Non Non Non
lactose fermenter lactose lactose lactose lactose
fermenter fermenter fermenter fermenter fermenter
SS Pink Pink yellow Yellow Yellow Pink Pink
medium medium medium medium yellow medium
yellow colourless black black medium yellow
colonies colonies colonies colonies colourless colonies
colonies
CLED Blue Blue Blue Blue Blue Blue
medium medium medium medium medium medium
colourless colourless colourless colourless colourless colourless
colonies colonies colonies colonies colonies colonies
Gram stain Gram Gram Gram Gram Gram Gram
negative negative negative negative negative negative
bacilli coccobacilli coccobacilli bacilli in cocci in coccobacilli
cluster cluster in chain

3. the important of the quality control for reagents and media


In my opinion, the importance of quality control for reagents and media is to
prevent the contamination occurs to the reagents and media. Plus, it is can prevent
the reagents and the media from damaged. Lastly, to make sure that the media is
prepared correctly.

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