HumaCount 30TS and 60TS

| Service Manual

16420/302
REVISION LIST OF THE MANUAL
Rev. /DATE REVISION DESCRIPTION
01/2010-09 First edition
02/2012-04 Revised structure and update
03/2012-06 Hardware update
04/2015-12 Addition Cabling Diagram

SYSTEM VERSION

HumaCount 60TS and HumaCount 30TS with software version 2.0 and 2.1

COPYRIGHT

Copyright 2012, Human GmbH, Wiesbaden, Germany. All rights reserved.

No part of this documentation may be reproduced in any form, nor processed, copied or
distributed by means of electronic systems, without prior permission of Human GmbH in
writing. Since all precautionary measures were taken into account in producing these operating
instructions, the manufacturer accepts no responsibility for any errors or omissions. This
includes any liability for damage that could arise from possible incorrect operation based on this
information. Subject to changes without notice as result of technical development.

SERVICE UND SUPPORT

CONTENTS

TABLE OF CONTENTS

1 SAFETY INSTRUCTIONS 5
1.1 INTRODUCTION 5
1.2 USER WARRANTY 5
1.3 INTENDED USE OF THE INSTRUMENT 6
1.4 GENERAL SAFETY WARNINGS 6
1.5 DISPOSAL MANAGEMENT CONCEPT 7
1.6 INSTRUMENT DISINFECTION 7
1.7 BIOHAZARD WARNING 8

2 SYSTEM DESCRIPTION 11
2.1 NAME AND SERIAL NUMBER 11
2.2 INTEGRATED SOFTWARE 11

3 INSTALLATION OF THE INSTRUMENT 13

4 REMOVAL OF THE INSTRUMENT 15

5 MAINTENANCE 17
5.1 SELF TEST 17
5.2 DEVICE STATISTICS 17
5.3 CLEANING AND GREASING DILUTOR BLOCK 17
5.4 CHECKING AND LUBRICATING DILUTOR PISTON TIPS 17
5.5 MICRO PISTON SEALING 17
5.6 CHECKING AND REPLACING WASHING HEAD 17
5.7 BLEACHING OF FLUIDIC SYSTEM 18

6 TROUBLE SHOOTING 19
6.1 SELF TEST 19
6.1.1 Selftest Screens 19
6.1.2 Actions in case of failed self test parameters 21
6.2 DEVICE INFORMATION 22
6.3 LOG IN AS SERVICE 22
6.4 SERVICE MENU 24
6.4.1 Troubleshooting menu 24
6.4.2 Stress 25
6.4.3 Needle position check 26
6.4.4 Statistics 26
6.5 AMPLIFIER SETTING 27
 6.5.1 Setting RBC amplifier gain 27
6.5.2 Setting WBC amplifier gain 27
6.6 POSSIBLE CAUSES OF NOISE 27
6.6.1 Contaminated reagent 28
6.6.2 Bad earth grounding 28
6.6.3 External electrical noise 28
6.6.4 Internal noise sources 29
6.7 WARNING FLAGS 31

7 ELECTRONICS 33
7.1 COUNTING CHAMBER WITH ELECTRODES AND MEASURING TUBE 36
7.2 HGB MEASURING HEAD 36
7.3 CELL COUNTER AMPLIFIER BOARD 37
7.3.1 HC30TS 37
7.3.2 HC60TS 39
7.4 MAIN CPU BOARD 40
7.5 DIMM-PC* MODULE 42
7.6 DIMMBOARD MODULE 43
7.7 OPTO SENSORS 43
7.8 VALVE ASSEMBLIES 43
7.9 FRONT PANEL ELECTRONICS 44
7.10 EXTERNAL POWER SUPPLY 44

8 MECHANIC AND FLUIDIC PARTS 45

8.1 SAMPLE PROBE MECHANICS 46
8.1.1 Vertical movement, setting timing belt tension 47
8.1.2 Vertical opto sensor and needle settings 48
8.1.3 Setting the horizontal movement 49
8.1.4 Setting the needle shaft 50
8.1.5 Setting the position of the chambers 50
8.2 SAMPLE PROBE 51
8.3 WASHING HEAD 51
8.4 PUFFER RESERVOIR 51
8.5 DILUTOR BLOCK 52
8.5.1 HC30TS 52
8.5.2 HC60TS 53
8.6 MEASURING BLOCK 54
8.6.1 HC30TS 54
8.6.2 HC60TS 54
8.7 PUMP 55
CONTENTS

8.8 ASSEMBLED ANALYZER 55

8.8.1 Front Panel 55
8.8.2 Rear Panel 56
8.8.3 Front view inside 56
8.8.4 Side view inside 57

9 OPERATION OF THE FLUIDIC SYSTEM 59

9.1 INITIALIZATION OF THE FLUIDIC SYSTEM 59
9.2 OPERATION OF THE FLUIDIC SYSTEM 60
9.2.1 HC30TS 60
9.2.2 HC60TS 67

10 MISCELLANEOUS 75
10.1 SERVICE CALIBRATION 75
10.2 MDA SCREEN 75
10.2.1 Event log 76
10.2.2 Command input 76
10.3 USB B CONNECTOR COMMUNICATION 76
10.3.1 Characters and basic structure 76
10.3.2 Details of the 3.1 protocol 77
10.4 CABLING DIAGRAM 80
10.5 TUBING SCHEMATICS 81
10.6 RECOMMENDED KIT OF TOOLS 83
Safety Instructions 5

1 SAFETY INSTRUCTIONS

1.1 Introduction
This manual is considered as a part of the instrument; it has to be at the oper-
ator’s hand as well as at the maintenance operator’s availability. For accurate
installation, use and maintenance, please read the following instructions care-
fully. In order to avoid instrument damage or personal injury, carefully read the
”GENERAL SAFETY WARNINGS”, describing the suitable operating procedures. In
case of breakdowns or any troubles with the instrument, apply to the local Tech-
nical Service.

1.2 User warranty

HUMAN warrants that instruments sold by one of its authorised representa-
tives shall be free of any defect in material or workmanship, provided that this
warranty shall apply only to defects which become apparent within one year
from the date of delivery of the new instrument to the purchaser.
The HUMAN representative shall replace or repair any defective item at no
charge, except for transportation expenses to the point of repair.
This warranty excludes the HUMAN representative from liability to replace
any item considered as expendable in the course of normal usage, e.g.: lamps,
valves, syringes, glassware, fuses, diskettes, tubing etc.
The HUMAN representative shall be relieved of any liability under this warranty
if the product is not used in accordance with the manufacturer‘s instructions,
altered in any way not specified by HUMAN, not regularly maintained, used with
equipment not approved by HUMAN or used for purposes for which it was not
designed.
HUMAN shall be relieved of any obligation under this warranty, unless a
completed installation / warranty registration form is received by HUMAN with-
in 15 days of installation of this product.
This warranty does not apply to damages incurred in shipment of goods. Any
damage so incurred shall be reported to the freight carrier for settlement or
claim.
equipment not approved by Human or used for purposes for which it was not
designed.
Human shall be relieved of any obligation under this warranty, unless a com-
pleted installation / warranty registration form is received by Human within
15 days of installation of this product.
 6

This warranty does not apply to damages incurred in shipment of goods. Any
damage so incurred shall be reported to the freight carrier for settlement or
claim.

1.3 Intended use of the instrument

[IVD] The instrument is intended for in vitro diagnostic application by professional
users. It has to be used for the expected purposes and in perfect technical
conditions, by qualified personnel, in working conditions and maintenance
operations as described in this manual, according to the GENERAL SAFETY
WARNINGS. This manual contains instructions for professional qualified
operators.
HumaCount 30TS / HumaCount 60TS hematology analyzers are fully auto-
mated cell counters for in vitro diagnostic use. The compact instruments were
developed for small clinics, point-of-cares, and hospitals.
HumaCount 30TS can process 30, HumaCount 60TS can process 60 samples
per hour and they are intended to determine the following 18 hematology
parameters from a 25µL whole blood sample:
-- WBC - LYM - MON - GRA - LYM% - MON% - GRA% (three-part WBC
differential)
-- HGB - RBC - HCT - MCV - RDW - MCH - MCHC
-- PLT - MPV - PCT – PDW

1.4 General safety warnings

Use only chemical reagents and accessories specified and supplied by HU-
MAN and/or mentioned in this manual. Place the product so that it has proper
ventilation.
The instrument should be installed on a stationary flat working surface, free
from vibrations.
Do not operate in area with excessive dust.
Work at room temperature and humidity, according to the specifications listed
in this manual.
Do not operate this instrument with covers and panels removed.
Only use the power cord specified for this product, with the grounding
conductor of the power cord connected to earth ground.
Use only the fuse type and rating specified by the manufacturer for this instru-
ment, use of fuses with improper ratings may pose electrical and fire hazards.
To avoid fire or shock hazard, observe all ratings and markings on the
instrument.

HumaCount 30TS / 60TS | Service manual

Safety Instructions 7

Do not power the instrument in potentially explosive environment or at risk of

fire.
Prior to cleaning and/or maintaining the instrument, switch off the instrument
and remove the power cord.
For cleaning use only materials specified in this manual, otherwise parts may
become damaged. It is recommended always to wear protective apparel and
eye protection while using this instrument. Respective warning symbols, if
appearing in this manual, should be carefully considered.

1.5 Disposal management concept

The currently valid local regulations governing disposal must be observed. It is in
the responsibility of the user to arrange proper disposal of the individual
components.
All parts which may comprise potentially infectious materials have to be
disinfected by suitable validated procedures (autoclaving, chemical treatment)
prior to disposal. Applicable local regulations for disposal have to be carefully
observed.
The instruments and electronic accessories (without batteries, power packs etc.)
must be disposed off according to the regulations for the disposal of electronic
components.
Batteries, power packs and similar power source have to be dismounted from
electric/electronic parts and disposed off in accordance with applicable local
regulations.

1.6 Instrument disinfection

Analytical instruments for in vitro diagnostic involve the handling of human
samples and controls which should be considered at least potentially infectious.
Therefore every part and accessory of the respective instrument which may have
come into contact with such samples must equally be considered as potentially
infectious.
Before doing any servicing on the instrument it is very important to thorough-
ly disinfect all possibly contaminated parts. Before the instrument is removed
from the laboratory for disposal or servicing, it must be decontaminated.
Decontamination should be performed by authorised well-trained personnel
only, observing all necessary safety precautions. Instruments to be returned
have to be accompanied by a decontamination certificate completed by the
responsible laboratory manager. If a decontamination certificate is not sup-
plied, the returning laboratory will be responsible for charges resulting from
 8

non-acceptance of the instrument by the servicing centre, or from authority’s

interventions.

1.7 Biohazard warning

Analytical instruments for in vitro diagnostic application involve the handling
of human samples and controls which should be considered at least potentially
infectious.
Therefore every part and accessory of the respective instrument which may have
come into contact with such samples must equally be considered as potentially
infectious.
For safety reasons, we have labeled instruments with the „BIOHAZARD“
warning label below.

Figure 1
Biological Hazard Symbol

HumaCount 30TS / 60TS | Service manual

Safety Instructions 9

Notes:
 10

HumaCount 30TS / 60TS | Service manual

System Description 11

2 SYSTEM DESCRIPTION
Since HumaCount 30TS and HumaCount 60TS have so much common character-
istics, we issue a common Service Manual covering both instruments. Informa-
tion herein applies for all instruments unless otherwise noted.
To be well up in the instruments, please read this manual carefully to have the
knowledge for servicing the instruments perfectly and avoid extra costs and
wasting precious time.
In this manual, we are using the following conventions:

HC30TS – stands for HumaCount 30TS

HC60TS – stands for HumaCount 60TS

This HumaCount 30TS / HumaCount 60TS Service Manual contains the functional
descriptions of all analyzers, operation of the fluidic systems, adjustments and
settings, and very important information for the service personnel about the
service operations and possible problems.

2.1 Name and serial number

Name: HumaCount 30TS / HumaCount 60TS Hematology Analyzer
Every instrument has its own serial number, which is printed
on the rear panel label and it can be read out from Device In-
Serial No.:
formation or from the self test submenu. This identity number
is write-protected.

2.2 Integrated software

The integrated software controls the instrument operations, displays, stores,
recalls data, and allows the user to perform QC and calibration procedures and
modify the user settings. The software version number can be read out from the
Device Information or from the Self test submenu.
Every HC30TS / HC60TS software version is upgradeable (using a USB flash drive)
by the latest program developed by HUMAN, and it can be downloaded from:
http://www.human.de
 12

HumaCount 30TS / 60TS | Service manual

Installation of the instrument 13

3 INSTALLATION OF THE INSTRUMENT

For the installation of the instrument please refer to the relevant user manual.
 14

HumaCount 30TS / 60TS | Service manual

Removal of the instrument 15

4 REMOVAL OF THE INSTRUMENT

To remove the instrument for e.g. service it must be shutdown according to the
instructions given in the user manual for turning the instrument off, preparing
for shipment. For transport make use of the original packaging material to pro-
tect the instrument from damage.
Disposal has to be done in accordance with local regulations.
 16

HumaCount 30TS / 60TS | Service manual

MAINTENANCE 17

5 MAINTENANCE
The instruments should be checked and maintenance must be carried out every
6 months, or after 10 000 measurement cycles.

5.1 Self test

Run the built-in Self test and check the overall test result. Regarding failed self
test see section „6.1.2 Actions in case of failed self test parameters“ on page
21.

5.2 Device statistics

Check the device statistics to find common problems.
For details see section „6.3 Log in as SERVICE“ on page 22 and section „6.4.4
Statistics“ on page 26

5.3 Cleaning and greasing dilutor block

The dilutor block driving wheels and gear bar should be cleaned from dirt and
must be greased between the gear bar and the support, and between cogged
wheels. Use the grease in the red/orange Eppendorf cup (Machine grease LX2)
for the cogged wheel connections and the white grease in the clear Eppendorf
cup (Photolube) for sliding bars. Both are part of the ‚Lubrication Kit‘.

5.4 Checking and lubricating dilutor piston tips

The cogged end of PTFE dilutor pistons should be cleaned and lubricated. Apply
just a thin layer, and move it along the perimeter of the piston, so that some of
the material goes into the gaps between the sealing rings. Use the clear silicon
grease in the blue Eppendorf cup which is part of the ‚Lubrication Kit‘.

5.5 Micro piston sealing

Check the condition of the micro piston sealing, and replace if necessary.

5.6 Checking and replacing Washing Head

Check the state of the washing head, and replace if necessary. After replacing
washing head, do not forget to perform correct adjustment of sampling nee-
 18

dle height (see section „8.1.2 Vertical opto sensor and needle settings“ on page
48).

5.7 Bleaching of fluidic system

It is recommended to run a bleaching procedure to remove stains from the
fluidic system.

1. Connect 2-5%, hand warm, clean bleach solution to all reagent inputs.
2. Perform a stress test with 3 cycles.
3. Connect D.I. water to all inputs immediately after the 3 cycles are completed.
4. Immediately after connecting D.I. water perform a stress test with 5 cycles.
5. Connect the reagents to the reagent inputs again.
6. Perform a stress test with 5 cycles.

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Trouble shooting 19

6 TROUBLE SHOOTING

6.1 Self test

There is a built-in Self test in each model. The Self test can be
used to check the operation of the instrument. It can be select-
ed by Maintenance, Diagnostics, Selftest. By pressing the Start
button the selftest will be performed. The test results can be
printed or saved to USB flash disk.

6.1.1 SELFTEST SCREENS

To see all results of the self test you need to scroll down.
Every measured value has a check mark if it is in the acceptable
range, or an X and a minus or plus sign if it is below or above
the normal range.
Overall result indicates Success only if all individual results are
within acceptable range. Otherwise it will show Failed.
HGB measured impulses per second
Vacuum reports pump operation (vacuum made by the pump
in a 10-second period of time).
Drift represents pressure loss of vacuum measured in a 10-sec-
ond period of time.
Measuring Electrode voltage, current and offset.

Amplifier Noise test during a 5-second period.

Amplifier test by generating 20000 test pulses, incl. gain relat-
ed Peak value, noise related Deviance.
 20

Fast blank measure, the device performs a fast blank measure-

ment. This number is the PLT count. Fast blank probe min and
Fast blank probe max voltage are relative numbers during fast
blank measurement. For HC30TS this is single measurement
while for HC60TS separate results are shown for both measure-
ment chambers.

Following the normal ranges of the different parameters for

the selftest are listed:
Table 1 Parameter Unit Lower bound Upper bound
Normal range of self HGB count 3000 60000
test parameters Vacuum mBar 300 600
Drift mBar/10sec 0 10
Electrode voltage V 45 55
Electrode offset mV -5.0 5.0
Electrode current µA 830 930
Noise test pls/5sec 0 50
Amplifier test count 19990 20050
Peak mV 1300 1700
Deviance mV 0 100
Fast blank meas count 0 100
Fast blank probe
- 280 360
min
Fast blank probe
- 280 360
max

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Trouble shooting 21

6.1.2 ACTIONS IN CASE OF FAILED SELF TEST PARAMETERS

Following recommended actions are given in case of failed parameters of the
self test:
Parameter Mark Possible reason Remedy Table 2
Check HGB LED during Recommended actions in
HGB head not connected measurement. case of failed self test
or HGB LED or sensor out Check light pathway through
Low
of order. Pptical pathway chamber. Check HGB head
HGB disturbed. electrical connection. Replace
if necessary.
Stray light or HGB LED
Close door or check optical
High too bright or HGB head
pathway. Replace if necessary.
damaged.
Pump or sensor failure or Check function of pump and
Vacuum High
leakage sensor. Check for leakage.

Drift High Leakage in pneumatics Check tubing in pneumatics.

Electrode Low or Fault on Main or Change Main or Amplifier

voltage High Amplifier board board
Electrode Low or Fault on Main or Change Main or Amplifier
offset High Amplifier board board
Electrode Low or Check measuring circuit.
Fault on Amplifier board
current High Measuring tube clogged?
Noise test High Instrument not grounded Check mains ground lead
Amplifier Boards is not Check cables and connectors
Low
Amplifier test connected to Main board coming from the Amplifier

High Instrument not grounded Check mains ground lead

Low or Change Main or Amplifier
Peak Fault on Amplifier board
High board
Deviance High Instrument not grounded Check mains ground lead
Fast blank
High Contaminated system Run cleaning cycle
measure
Check measuring circuit for in-
Fast blank High or terruptions. Check for correct
Fault on MAIN or
probe min Low liquid in chamber (diluent).
Amplifier board or
Change depending on obser-
measuringcircuit
vation: chamber, measuring
disturbed
Fast blank High or tube, reference elctrode, ca-
probe max Low bles, Amplifier or Main board.
 22

6.2 Device information

Under Maintenance, Diagnostics you will also find the function
Device information. This function displays in a short form the
serial number of the instrument and the versions of the differ-
ent software.

6.3 Log in as SERVICE

To log in as Service procede as follows:
1. In Main menu touch Exit button.

2. Touch Logout (USERNAME).

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Trouble shooting 23

3. Press Login

4. At Log In screen press the START button on the front panel,

so the Login name will change to Service.
5. Touch the Password field.

6. Type in the Service User password which is 6484 and touch

Enter to confirm.

7. Touch Accept button in Log in screen.

 24

The display will show the Exit menu again. By touching Home
the Main menu will be shown again. Touch Maintenance, Diag-
nostics and Service to enter the Service menu.

6.4 Service Menu

The Service menu is intended for servicing and operation
checking purposes.
It can only be accessed by user Service.

6.4.1 TROUBLESHOOTING MENU

The Troubleshooting menu provides tools to test different
components of the instrument.

6.4.1.1 Motors
From the Motors submenu each or all motor movements can
be checked.
By testing the movements the optos are also checked since the
end points of a movement in one direction is dependent on the
corresponding opto switch. However a separate testing of the
optos might be necessary.
While the motor test is running the progress will be displayed
on the screen.

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Trouble shooting 25

6.4.1.2 Optos
Optosensor states can be checked in this screen.
The mechanical parts must be moved manually for this test.
By moving the flags/holes in and out of the optos the state of
the correspondig opto switch can be observed on the screen by
the change of the colour of the square box between black and
white.

6.4.1.3 Valves
This menu allows to check the correct function of the valves
and the pumps.
Pressing the buttons of the valves switches them. 1 stands for
electronically forced state.
By pressing the Pump(s) button the peristaltic pump(s) can be
switched on/off.
The current vacuum in the puffer reservoir is displayed under
the buttons.

6.4.1.4 Amp. Offset

You can check the offset on the amplifier board. The current
offset and the acceptance range are displayed.

6.4.2 STRESS
In Stress mode, the instrument performs measuring cycles
without sample (blank measurements) continuously. This can
be used for burn-in tests, or to check the pneumatic system af-
ter changing any main fluidic part.
 26

You can have information about stability, cleanliness, HGB op-

eration, and counting time stability. Results of the PLT and HGB
blank results are displayed in table format.
You can detect any kind of noise, or bubbles in the system if the
PLT is not stable low, or HGB has big variation.
To start stress cycles press Start or Start 10. Start will start
stress cycles until the process is stopped. Start 10 will perform
10 stress cycles.
The Stress test can be stopped anytime by pressing Abort.

6.4.3 NEEDLE POSITION CHECK

With Needle position check the correct height of the needle
can be set. If the needle opto is set correctly, the lower end of
the needle is co-planar with the bottom plane of the washing
head. If it doesn’t then adjust the needle opto up or down and
check the position again (see section „8.1.2 Vertical opto sensor
and needle settings“ on page 48).

6.4.4 STATISTICS
In this screen the statistics of different actions and events is
shown. This helps to identify issues which need attendance.
Once checked for service issues press Clear to reset the count
to zero.

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Trouble shooting 27

6.5 Amplifier setting

The following possibilties to set the amplifier gain are only
available if the service engineer is logged in as Service (see sec-
tion „6.3 Log in as SERVICE“ on page 22). The settings are
under Settings, Measurement, Settings, Calibration.

6.5.1 SETTING RBC AMPLIFIER GAIN

If the correct MCV value cannot be obtained by calibration
the amplifier gain of RBC measurement could be increased
or decreased by approx. ±10%. Please be advised that chang-
ing this value requires to recalibrate the device to get proper
results!

6.5.2 SETTING WBC AMPLIFIER GAIN

If the WBC diagram is shifted too far on left or right in WBC
histogram the amplifier gain of WBC measurement could be
increased or decreased by approx. ±10%. Please be advised that
changing this value requires to recalibrate the device to get
proper results!

6.6 Possible causes of noise

Generally high count of any particle - even if you think it should
be low, or near zero - can be caused by noise, i.e. something
interfere with the measurement.
The most important thing in these cases is to identify the
source of the noise, otherwise you cannot protect the system
against it.
Noise can come from several sources, and the different sources
add up. However only one of them may be enough to cause
problems.
 28

6.6.1 CONTAMINATED REAGENT

The most probable cause are particles in the reagent, and therefore the PLT
blank is continuously high (e.g. always 30-40). You can easily sort out this case
by replacing diluent with a new container. PLT blank must go down during
several blank measurements (below 10).
How can a good reagent become bad with time?
-- If the reagent tube was contaminated, and some bacteria begin to grow
inside, once you put an infected reagent tube into a new tank, by time it
can become infected as well, i.e. the background (PLT blank) becomes high.
Wash the reagent tube - which is in connection with the reagent - with 1% of
bleach solution, then rinse with clean distilled water or diluent. It can avoid
the bacteria to grow inside.
-- If container is open – and cap is not installed or closed - external dust can fall
into the reagent.

6.6.2 BAD EARTH GROUNDING

In this case external - ground referenced - noise can get into the system by
ground coupling. If system ground is not good enough, ground terminal can be-
come a noise source as well, i.e. external signals will be coupled into the system
instead of protecting it.
If no earth ground is available, you can use a screw at the rear panel to connect a
ground potential to the case, so that noise immunity can be increased.
Measure voltage on ground terminal to make sure earth grounding is correct. AC
voltage lower than 1V is accepted in this case.
At some places - as a bad practice - electricians like to connect earth ground
terminal to neutral wire. Depending on the resistance of the neutral back
wire (where it is really earthed), several volts can appear, and this way any
inductive noise will be coupled into the instrument. It is better to create a real
earth grounding and connect it to the rear screw.

6.6.3 EXTERNAL ELECTRICAL NOISE

If another instrument is near the analyzer can radiate electromagnetic sig-
nals in the 1 kHz - 100 kHz frequency region it can be picked up by the system
(especially if they are very close to each other, or the grounding is not quite per-
fect).
You can easily identify this noise source: by relocating the instrument
noise (high PLT blank) disappears. In this case you have to identify the possible
noise source (switch mode power supplies, computer monitors, since they are

HumaCount 30TS / 60TS | Service manual

Trouble shooting 29

not shielded, centrifuges due to high switching noise of rotor contacts, etc.), the
power of the electromagnetic source, because if high power is present, maybe
relocation does not solve your problems, sometimes the electric power supply
makes the coupling, so UPS solves the problem.
Another source of coupling in external noise can be the reagent tanks and tubes.
Especially radio transmitters can cause problems of radiating so that even the
reagents (diluent) guides in the noise. A metal pack for the diluent tank, then
a good earth grounding of this metal box allows this coupling to disappear
forever.

6.6.4 INTERNAL NOISE SOURCES

The most annoying but real cause is some sort of internal noise. The reason for
this phenomenon is that inside electrode - hot point - of the measuring circuit
must be well insulated from surrounding electronics, otherwise inside noise
sources can take their effect.

6.6.4.1 Bad chamber insulation

-- Bad shielding of the chamber (floating shield couples signals to the
chamber, and does not prevent against them). Check grounding of shield,
remove it and clean the surface between the shield and the metal base.
-- Bad reference electrode connection (floating ground reference). Repair is
required.
-- Bad sealing of aperture. Replacement of measuring tube is required.
-- Broken measuring chamber starts to conduct through the gaps (ground
path). Replacement of chamber is required.
-- Contaminated draining tube starts to conduct due to protein or lipid build-
up. It is very easy to identify this case. After replacing the drain tube of the
measuring chamber (mainly WBC), WBC histogram peak, or PLT becomes
low soon. Normally a good cleaner is required to dissolve lipid or protein
build-up. Sometimes the cleaner is not strong enough to keep this tube clean
enough. Periodic washing using 1% hand warm bleach solution helps.

6.6.4.2 Bad insulation of electronic signal paths:

In these cases check for any capacitive coupling of electronic signals to the
chamber:
-- Interference with HGB head (high-frequency signal is coupled to the cham-
ber). HGB head metal parts must be grounded. The ground comes externally,
it must be in place, otherwise HGB head does not shield, but couples in noise.
 30

-- Interference with internal high voltage inverter (high-frequency signal is

coupled to the chamber). Repair is required: avoid near contact of HVB cable
to chamber or shielded amplifier cable.
-- Interference with internal start button (polling signal to start button may
cause noise). Guide start button wires as far from chamber as possible. You
may try mix them up on the start micro-switch if applicable.
-- Interference with display cable (high-frequency LCD signal is coupled to the
chamber by the ribbon cable). Keep the ribbon cable far from the chamber.
-- Interference with CPU fan or other digital logic traces (CPU fan or other
digital signal radiates to chamber or to the shielded amplifier cable). Try
keeping the ribbon cables far from the chamber and shielded cable.

6.6.4.3 Bad components or connections:

-- Bad soldering, salt residuals or component failure on amplifier (especially
if some reagent could get in the amplifier section). Cleaning of PCB/elec-
trode socket or replacement of amplifier is required. Check for the correct
soldering of reference cable and its connector.
-- Circuit board bad soldering or component failure. Check the shielded cable
connections as well. Sometimes inside out connection (hot electrode goes
outside as a shield) is the problem: both ends of amplifier signal cable must
be reversed.
-- Analog signal ribbon cable (it picks up noise). Check the ribbon cable
between the circuit board and the amplifier. Maybe it is pinched under some
screws or components. This may cause trouble and even noise.

6.6.4.4 Pneumatic failures, liquid paths that conduct noise into the chamber:
-- Liquid remains under the chamber in drain tube (during measurement the
conducting liquid remains inside the drain tube making noise to appear
there).
• Check chamber draining path for clogging or salt crystals.
• Check the pump operation. Since draining of the chamber goes under
pressure control, maybe a bad pressure sensor or connection can cause
trouble.
• Clean the draining path. Do not use alcohol, but bleach. Replace chamber
if necessary.
-- Liquid remains in the washing inlet at top of the chamber (during measure-
ment the conducting liquid remains inside the chamber wash tube making
noise to appear). The software is not compatible with the mechanics, or re-
lated valve is bad/partly clogged, or the tubing is clogged/loose.

HumaCount 30TS / 60TS | Service manual

Trouble shooting 31

-- Lyse path guides in noise (during counting, if the a liquid in the draining tube
is touching lyse reagent in T-fitting, noise can appear). Check the lyse path,
and the lyse valve as well.

6.7 Warning flags

Analyzer SW displays warning flags for each individual measurement to noti-
fy user about status of results. The following table summarizes warning flags
and gives explanation of their possible cause and a few hints to overcome the
problem.
Uppercase letters refer to WBC or HGB problems.

Flag Meaning Recommended user action

Table 3
No WBC 3-part differ- Possible lyse problem. May occur in pathological Summary of warning flags related
E
ential lymphocytosis. to WBC/HGB

Repeat the blank measurement. If HGB blank is

HGB blank is high, or not stable there are probably bubbles in the WBC
H
no HGB blank chamber: Run a cleaning and try blank again.
Close the side door if open during measurement.
Repeat the blank measurement, or run prime lyse
WBC blank is high, or
B and try blank again. Possible lyse contamination,
no WBC blank
or noise problem.
The analyzer found that the cell count is higher
linearity range ex- than the linearity range of the analyzer. Make a
M
ceeded in WBC stage pre-dilution, and run the same sample in predilut-
ed mode.
RBC cells were detected during the WBC meas-
RBC cells found in
urement. Either the lyse reagent is not effective
R sample during WBC
enough (volume should be increased) or the RBC’s
stage
in the sample are somewhat lyse resistive.

Probably large PLTs or clumped PLTs are present

in the sample. Usually caused by the nature of the
sample. cat and goat samples tend to clump. In-
tensive, but careful mixing of the sample (e.g. Vor-
W WBC 3-part warning
tex) can help remove the clumps. If the rerun sam-
ple gives the same results, consider that WBC and
NEU values seem higher because of the clumps.
Lyse modification can’t solve the problem.
 32

Typically insufficiently lysed RBC’s interfere with

the start of the WBC histogram. Repeating the
measurement with an increased lyse volume
RBC-WBC limit warn-
L should provide better separation. If the repeated
ing
run reports very similar results then the MON and
NEU results are VALID but the WBC and LYM re-
sults may be higher because of interfering RBCs.
Aperture clogging. Perform cleaning and repeat
the measurement. If it is a general problem,
please contact your Service Personnel. Low tem-
C WBC clogging
perature reagents can cause it as well (mainly
diluent), in this case you will have to wait until
they reach room temperature.
Warning flags in lowercase refer to RBC or PLT problems.

Flag Meaning Recommended user action

Table 4
The analyzer found that the cell count is higher
Summary of warning flags Linearity range
than the linearity range of the analyzer. Make a
related to RBC/PLT m exceeded in PLT/RBC
predilution, and run the same sample in predilut-
stage
ed mode.
Multiple or incorrect RBC peak(s) detected. Try to
k RBC peak error
run the sample again.
PLT and RBC cells could not be separated, or the
PLT / RBC limit not
l histogram remained high in the PLT/RBC valley
correct
range.
c RBC/PLT clogging The same action as in case of the C warning flag.
Run cleaning and repeat the blank measurement
PLT blank is high, Diluent or system cleanliness problem. If it is sta-
p
or no PLT blank ble high, replace the diluent by opening a new
tank.
RBC blank is high or
b Same action as in case of warning flag p.
no RBC blank

HumaCount 30TS / 60TS | Service manual

Electronics 33

7 ELECTRONICS
HC30TS / HC60TS contain the following electronic parts:

1. Counting chamber (2 pcs in HC60TS) with electrodes and measuring aper-

ture
2. HGB Measuring Head
3. Cell Counter Amplifier Board
4. MAIN CPU Board with Dimm-PC and measurement processing unit, 4 mo-
tor controllers, valve & pneumatic controller/driver, pump driver and power
supply for internal printer (+7.5V) and digital circuitry (+5V, +3.3V)
5. DIMM-PC module
6. Motors with opto-boards of needle moving motor (H) and sample rotor/nee-
dle moving motor (V)
7. Dilutor block with opto-board for sampling, diluent, lyse and cleaner
8. Valve boards
9. Peristaltic Pump (2 pcs in HC60TS)
10. USB interface
11. Graphic LCD Display Module with touch-screen
12. Start Button Panel
13. Internal Printer
 34

Figure 2
Electroics block diagram

HumaCount 30TS / 60TS | Service manual

MAIN Board (CPU, Pneumatic and Power Board) Fluidic System
Amplifier Board
Internal printer
interface
Motor driver 1 Rotor motor Counting chamber
(MIX/WBC in Cell counter Amplifier
HC60TS)
Motor driver 2 Motor driver 1
12 VDC
external Power Dilutor motor( 2 in HGB measuring head
Supply Motor driver 3 HGB interface
HC60TS)

Motor driver 4 (used

in HC60TS only) Snap in optosensors
RBC chamber
Digital power 5V (in HC60TS only)
Opto sensor interface
Valve block I _II

External printer
Digital power 3.3V Valve drivers
Pump

Pump driver

USB A 1
FPGA Pressure sensor
XCS30XL
USB A 2
External keyboard Measure control
DIGIO Display
USB A 3 Assembly
HVB(50V, 150V)
USB A 4
USB stick
DC/DC ±12V
320*240 (QVGA)
USB DIMMPC TFT modul
HUB AMD Elan SC-520 Start
PIC24 w/ touchscreen
μController To PIC ADC button & w/ LED backlight
Status
LED board

USB B
LCD backlight driver
Sign Collection Board
External PC
BLTS v1.0
Touchscreen
interface

Start Button, Status

LEDs interface

LCD driver
 Valve board Valve board P1 Built-in Printer Vertical optosensor
1-5 6-11 16350/184
Electronics

1-10 10-20 Sample rotor/Horizontal

movement optosensors

Valves cable 16420/176 Left Right

13-15

Dilu Vertical Horizontal

Motor (3) Motor (2) Motor (1)
M M M Printer cable 16420/179 1-3 4-6

Opto cable 16420/175

…... …... …... …...

USB6 USB4
HC30TS Front Panel Dilutor optosensors

Upper Lower

LCD flat cable

Front USB
16420/171 Internal USB cable 7-9 10-12
panel

Display 16420/157 Other side of

Power switch
the board
16420/619
Back light cable
Touch flat cable

Meas 2
Meas 1
BLTSST 16420/174

Power input
ACS Main board …...
…..
16420/121-1

This line is available in case of closed system

Amplif cable
16350/176
Digio cable 16420/328
USB panel Reag prot
16430/218 USB cable 4 pin panel
16430/169

Amplifier
board
16420/321

Size Cat. no. Drawing name Version

Drown by: Eszter Tóth A3 Humacount30TS cabling diagram V1.0
Released: 13.10.2015 Scale N/A CO#: Page 1/1
Figure 3
Cabling diagram
35
 36

7.1 Counting chamber with electrodes and measuring tube

Impedance method is used for determination of volume and number of cells.
In this method a known volume of dilution is drawn through a small aperture.
Constant current is passed through the aperture from one side to the other.
When a cell passes through the aperture, it causes a change in resistance, which
generates a voltage pulse.
The amplitude of the voltage pulse is proportional to the ratio of cell volume
per aperture volume. This is used to determine the volume of cells. The num-
ber of cells can be obtained by counting the pulses. In the HC30TS there is one
cell-counter probe: the aperture size is 70 µm and has a reference electrode
assembly and U-shaped metal fixing as it is shown in the figure below.
The aperture is made of ruby and it is molded into the end of the measuring tube.
In the HC60TS there are two separate chambers: one for counting RBC with an
aperture of 80 µm, and another for MIX/WBC/HGB with 100 µm aperture.

Figure 4
Measuring chamber

1 8
1 Chamber
2 Electrode
3 Draining connection
7
4 Opening for measuring tube
2
5 Measuring tube
6 U-shaped metal fixing
7 Reference electrode
8 Washing inlet

3 4 5 6

7.2 HGB Measuring Head

Hemoglobin head is placed around one measuring chamber in all instruments.
It contains: light source (LED) at 540 nm wavelength and Photo Detector
(TSL235). The Photo Detector converts the light to frequency. The HGB concen-

HumaCount 30TS / 60TS | Service manual

Electronics 37

tration is a logarithmic function of this frequency measured by the FPGA circuit

on the MAIN board.

2 Figure 5
HGB measuring head

1 3
1 LED
2 Connection to amplifier
3 TSL 235

The frequency output signal of TSL235 is counted by a digital counter in the

FPGA circuit.
This counter counts up while the LED is on and counts down while the LED is
off. The LED and direction of counting are switched with a 100 Hz signal. This
method provides “real time backlight correction”, which makes the HGB meas-
urement more precise in changing backlight environment situation as well.
! Due to enhanced HGB tech-
nology, HC30TS / HC60TS is
less sensitive to incident light
There are two kinds of HGB measurement: changes. However, it is recom-
-- Sample measurement (before RBC counting) mended to keep side door closed
-- Diluent/blank measurement (in WBC washing phase) during measurements.
The HGB result is calculated from these measurements by:
HGB ≅ log (CNTdiluent light / CNTsample light)

7.3 Cell Counter Amplifier Board

7.3.1 HC30TS
Amplifier board includes its own voltage regulator, connection interfaces to
HGB head and to MAIN board. There is a current generator circuit on it, which
works from 50V measuring voltage (generated by MAIN) and the probe voltage
(DC) is amplified with a voltage follower (output: ELV). Nominal measuring cur-
rent is 870 µA.
 38

Figure 6
Amplifier board (front) HC30TS

3
1 Connection to DIGIO on
1
Main board
2 Connection to CSA1 on Main 2
board
3 Connection to HVB on Main
board

Figure 7
Amplifier board (back) HC30TS

1 Connection to electrode
2 Connection to HGB head

The amplifier board includes one input connector for the chamber (measuring
electrode). There are two opto switches (U1, U3) to connect high voltage to the
probe with HSW signal and isolate the input of the amplifier. Test circuit makes
possible to generate test pulses (with TEST and PLS signals through FETs) for
checking the proper operation of the amplifier channel.
Amplifier board includes a 3-stage main amplifier channel, which gains input
signal to the 0...3.3 V range (this is the input range of the A/D converter, which
is placed on the MAIN board). The RSW signal changes the gain (RBC, WBC) in
the feedback of the second amplifier stage with U2 (MAX319) analog switch.
Amplifier gain and offset are adjusted by software.
DHON signal switches on the LED and the MVON signal – which is active during
counting – switches off the Photo Detector in the HGB head, to prevent noise
generated by the HGB detector.

HumaCount 30TS / 60TS | Service manual

Electronics 39

The other side of the amplifier board contains special connectors for the cham-
ber and the HGB head (JP4).

7.3.2 HC60TS
Amplifier board includes its own voltage regulators, connection interfaces to
HGB head, to chamber electrodes, high voltage and DIGIO connector to Main
board. There is a current generator circuit on this board, which works from 50V
measuring voltage (generated by the High Voltage Circuit on Main board) and
the probe voltage (DC) is amplified with a voltage follower (output: ELV). Nomi-
nal measuring current is 870 µA.
Amplifier board includes one input connector for each measuring chamber
(measuring electrodes). There is one opto switch (OPT1) and a relay (REL1) to
connect high voltage to one of the probes with HSW signal and to isolate the
input of the amplifier. Test circuit allows generating test pulses (with TEST and
PLS signals through Q1, Q2 FETs) for checking proper operation of each amplifier
channel.

Figure 8
3 Amplifier board (front) HC60TS
1

1 Connection to HVB on Main

board
2
2 Connection to DIGIO on
Main board
3 Connection to amplifier in
on Main board
 40

Figure 9
Amplifier board (back) HC60TS

1 Connections to electrodes
2 Connection to HGB head 1

Amplifier board includes a 3-stage main amplifier channel, which gains input
signal to the 0...3.3 V range (this is the input range of the A/D converter on the
Main board). The RSW signal (with Q8 transistor) changes the input electrode
through REL2 relay.
The bottom side of the amplifier board contains special connectors for the elec-
trodes and the HGB head (JP2).
DHON signal - from the MAIN board - switches on (with Q4) the LED and the PLS
signal switches off the Photo Detector in the HGB head, to prevent noise gener-
ated by the HGB detector.

7.4 MAIN CPU Board

This board contains:
-- DIMM-PC and measurement processing unit,
-- 4 motor controllers,
-- valve & pneumatic controller/drivers, pump driver(s)
-- power supply for internal printer (+7.5V) and digital circuitry (+5V, +3.3V)

MAIN board is responsible to control the instrument: contains the main power
regulator circuits, valve and motor driver circuits and other connections for the
fluidic and pneumatic system’s parts, responsible for the specific measurement
processing functions.
The central micro-controller with a FPGA and with several other digital chips
(buffers, decoder, multiplexer) handles the pneumatic system, displaying,
measurement and data management.
Power system: filtering the +12V Input and generates +3.3V (FPGA), +5V (Digital
power), +7.5V (Printer power). Filtered +12V is used for the power of motors and
valves.

HumaCount 30TS / 60TS | Service manual

Electronics 41

Motor drivers: 4 power drivers; Horizontal, Vertical/Sample rotor motors and

dilutor motors (2 in HC60TS) have separated ribbon cable connections.
Valve driver: consists two 8-bit, powered output shift registers (with built in
protection diodes) and there is one common ribbon cable connection for the
valve boards. The peristaltic pump has a separated power FET driver circuit for
more reliable operation.
Measurement processing: the A/D conversion made by the micro-controller it-
self, but several preprocessing steps (time limits, noise handling, pulse integra-
tion) taken by the external analog circuitry.

Figure 10
Main board (front)

1 DIMM-PC
1 2 Video connection to touch
screen
2
 42

14 13 12 11
Figure 11
Main board (back)

1
1 Pressure sensor or connector
to pressure sensor 2

2 Motor connectors
10
3 Front panel USB connector
3
4 Connector to Touch screen,
backlight and START button 4
5 Connector to amplifier
6 Internal battery
9
7 Power connector
8 Power switch connector
9 USB B type connector 8

10 USB A type connectors 5

11 Connectors to opto sensors
12 Pump connector #1 7

13 Pump connecotr #2 (only

used in HC60TS)
14 Valves connector
6

7.5 DIMM-PC* module

The MAIN board incorporates a credit-card sized PC, named DIMM-PC*. The
processor on the Dimm-PC is a 133MHz Pentium-class core, with 32Mbytes on-
board RAM, and 32Mbytes on-board flash. This is the SSD (Solid State Disk) of
the analyzer, so instrument software with all user settings, calibration, data-
base, etc. is stored on the DIMM-PC.
The DIMM-PC module is used in HC30TS up to serial number 820660 and in
HC60TS up to serial number 920427.
* DIMM-PC® is the Trade Mark of Kontron Embedded Modules GmbH

Figure 12
DIMM-PC

HumaCount 30TS / 60TS | Service manual

Electronics 43

7.6 DIMMBOARD module

In HC30TS from serial number 820661 and in HC60TS from serial number 920428
a DIMMBOARD is used instead of the DIMM-PC module.

Figure 13
DIMMBOARD

7.7 Opto sensors

Opto sensor snap-in modules are responsible for checking motor positions.
There are 6 opto sensors in HC60TS, and 5 in HC30TS (see cabling diagram).

Figure 14
Opto sensor

7.8 Valve assemblies

There are two kinds of valve assemblies: Valve board 1-5 and Valve board 6-11.
HC30TS has 5 valves, while HC60TS has 6 valves in Valve board 6-11. The valve
boards are connected to controller and driver chips which are located on the
MAIN board.

Figure 15
Valve assembly
 44

7.9 Front panel electronics

Figure 16
Front panel assembly

1 Colour LCD with touch

1
screen 9

2 Touchscreen connector
3 START button and status LED
connector 8

4 Ribbon cable from front

2 7
panel to Main board
5 Front USB connector
3 6
6 Front panel ground connec-
tor
7 LCD backlight connector
8 BLTS board
9 LCD video connector to Main
board

4
5

7.10 External power supply

The analyzer works with an external power supply. The figure below shows the
power supply unit generating 12VDC.
The power supply modules have an auto range input, which makes possible
to use them with 230V or 115V mains outlet and it has the CE and UL safety
certificate. The input socket of the power supply is a standard 3-terminal plug,
with power cable connection; the output is a coaxial power connector.

HumaCount 30TS / 60TS | Service manual

Mechanic And Fluidic PARTS 45

8 MECHANIC AND FLUIDIC PARTS

HC30TS and HC60TS Hematology Analyzers consist of the following mechanic
and fluidic parts:

1. Sample rotor
2. Sampling needle
3. Washing head
4. Sample/Horizontal moving unit
5. Micro Dilutor
6. Dilutor
7. Chamber
8. Cell-counter probe
9. Puffer reservoir
10. Pump
11. Valves
12. Tubing

V2 DilWash
Figure 17
1

V4 DilNeedle
3
1

2 1
V3 DilChamber
Fluidic scheme HC30TS
3

3
2

V5 LyseWbc DILUENT
1 2

3 2 1

Pressure
V1 Cleaner
Meter 3
P V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
WBC
1
3

Micro Dilutor Lyse Dilutor V8 Bubble

2

M3

Macro Dilutor HGB

2

V7 DrainAperture
M2
1

LYSE Ver
2

V6 DrainChamber
2 1
1

V10 DrainPuffer Sample/

M1 H motor

Pump

2 1 1 2 3-way
Stepper Motor 2 -way Valve
M1 Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On
 46

V2 DilNeedle V1 DilWash

1
2 1

Figure 18

3
3

2
Fluidic scheme HC60TS

1
V4 DilChamber

2
P V3 Cleaner
DILUENT
Puffer 2 1

Reservoir
V5 LyseWBC 3
V10
1 2
1 2
3 3

1 2
CLEANER
Diluent Diluent Lyse V7 Aperture

1
MIX
RBC
V9 WBC

2
Bubble
Diluent

Micro dilutor HGB

M3 M4 M2
LYSE Ver

2
2
V6 DrainChamber V8 Drainchamber

2
1
1

3
V11 DrainPuffer

1
Sample/
M1 H motor

Pump 1
Pump 2

2 1 1 2
3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE

8.1 Sample probe mechanics

The sample rotor unit uses a stepper motor. The rotor has opto sensors for po-
sitioning. A second stepper motor is used for vertical probe movement. An opto
sensor at the upper end is used to determine the home position.

Figure 19
Sample probe and door
mechanics

HumaCount 30TS / 60TS | Service manual

Mechanic And Fluidic PARTS 47

8.1.1 VERTICAL MOVEMENT, SETTING TIMING BELT TENSION

The timing belt tension could be set with positioning the vertical motor using
the oval holes in the mounting plate.
1. Untighten the screws which fix the vertical motor
2. Push the motor down. Tighten the screws.

Figure 20
Setting the belt tension

3. Verify the tension. The vertical rod side of the belt must be pushed towards
the rod. The distance between the rod and the belt must be 2-3mm.

Figure 21
Verifying the belt tension
 48

8.1.2 VERTICAL OPTO SENSOR AND NEEDLE SETTINGS

The vertical opto sensor should be set as follows:
The flag (2) mounted on the vertical needle moving mechanism must run freely
between the two parts (1) of the opto-sensor.
Figure 22
Home opto sensor and flag for
vertical needle movement

1
1 Opto sensor
2
2 Flag

The vertical position can be set by loosening the two mounting screws of the
opto sensor and moving it up or down. In the correct setting the end of the nee-
dle is coplanar of the lower plane of needle washing head. The vertical position
of the needle is checked in the Service menu (see section „6.4.3 Needle position
check“ on page 26). If the the end of the needle is not coplanar with the lower
plane of the washing head adjust the opto sensor accordingly.

Figure 23
Setting opto sensor for vertical
needle movement

HumaCount 30TS / 60TS | Service manual

Mechanic And Fluidic PARTS 49

8.1.3 SETTING THE HORIZONTAL MOVEMENT

8.1.3.1 HC30TS
The setting of the horizontal movement is correct, when the opto wheel is in the
position shown in the picture, then the aspirating needle bracket is as close to
the cantilever as possible.
Figure 24
Horizontal probe mechanics
setting HC30TS

8.1.3.2 HC60TS
The setting of the horizontal movement is correct, when the opto wheel is in the
position shown in left picture, then the aspirating needle bracket is as close to
the cantilever as possible.
Figure 25
Horizontal probe mechanics
setting HC60TS
 50

8.1.4 SETTING THE NEEDLE SHAFT

The needle shaft must be fastened. If it was loose it could be adjusted with the
set screw on top end of the shaft.

Figure 26
Needle shaft setting

8.1.5 SETTING THE POSITION OF THE CHAMBERS

After setting the needle position, the horizontal position of the chamber bracket
should be checked. The needle must not go down exactly in the center of the
chamber. Chamber bracket can be moved left or right if necessary.

Figure 27
Chamber position setting

HumaCount 30TS / 60TS | Service manual

Mechanic And Fluidic PARTS 51

8.2 Sample probe

The sampling needle is assembled in the H&V moving unit and it makes the
sample transfer. Correct setting of sampling needle according to the previous
sections is necessary and very important.

8.3 Washing head

The washing head is located at the bottom of the H&V moving unit and it is for
cleaning the outer surface of the sampling needle. This washing process is made
with diluent reagent and the fluid is drained by the pump.

Figure 28
Wash head

1 Diluent inlet
2 Waste outlet

8.4 Puffer reservoir

The glass puffer reservoir is directly connected to the pressure sensor.
During measurement, there is no pump activity, so the puffer reservoir main-
tains measuring vacuum stable.
The instrument measures relative pressure so measuring vacuum is independ-
ent of atmospheric pressure.

Figure 29
Puffer reservoir
 52

8.5 Dilutor block

8.5.1 HC30TS
In HC30TS this unit includes one dilutor stepper motor. The Micro dilutor
syringe makes the aspirating while the motor moves down. The syringes are
mechanically connected with a loose mechanism, so there is a phase along the
track, where the micro dilutor doesn’t move.

Figure 30
HC30 dilutor block
TS
1

1 Micro syringe 4

2 Positioners
3 Lyse syringe
2
4 Dliuent syringe

8.5.1.1 Setting the dilutor mechanics

The micro-dilutor’s movement must be set by the following procedure:
1. Push the dilutor pistons up as possible.
2. Fasten the set screw of the upper fixing ring.
3. Fasten the lower fixing ring’s set screw in the position shown in picture on
the left.

Figure 31
HC30 dilutor mechanics
TS

HumaCount 30TS / 60TS | Service manual

Mechanic And Fluidic PARTS 53

8.5.2 HC60TS
In HC60TS this unit includes two dilutor channels – one for diluent and sampling,
and another one for lyse and diluent reagents. There are two stepper motors,
4 opto sensors, five syringes and piston rods with gear transmission. The Micro
dilutor syringe makes the aspirating while the motor moves down. The syringes
are mechanically connected with a loose mechanism, so there is a phase along
the track, where the micro dilutor doesn’t move.

Figure 32
HC60TS dilutor block

1
3

4
1 Micro syringe

2
2 Positioners
3 Diluent syringes
4 Lyse syringe

8.5.2.1 Setting the dilutor mechanics

The micro-dilutor’s movement must be set by the following procedure:
1. Push the dilutor pistons up as possible.
2. Fasten the set screw of the upper fixing ring.
3. Fasten the lower fixing ring’s set screw in the position shown in picture on
the left.
Figure 33
HC60TS dilutor mechanics
 54

8.6 Measuring block

8.6.1 HC30TS
The measuring block contains all components, counting chamber, measuring
tubes, HGB head, draining tubes.

Figure 34
HC30 measuring unit
TS

8.6.2 HC60TS
The measuring block contains all components, counting chambers, measuring
tubes, HGB head, draining tubes.

Figure 35
HC60TS measuring unit

HumaCount 30TS / 60TS | Service manual

Mechanic And Fluidic PARTS 55

8.7 Pump
Pump generates regulated vacuum and drains the fluidic system. It is connected
to the MAIN board and it has its own driver circuit (Power FET). In HC60TS there
are two pumps.
The pump is maintenance free.
Figure 36
Waste and vacuum pump

8.8 Assembled Analyzer

8.8.1 FRONT PANEL

Figure 37
HCxxTS front panel

1 LCD
1
2 START button
3 USB socket
4 Sample door

3
 56

8.8.2 REAR PANEL

Figure 38
HCxxTS rear panel 2

1 Reagent and waste connec- 3

tors
2 Power switch
4
3 USB A connectors
4 USB B connector
5 Grounding connector 5

6 Power connector 12V DC 6

8.8.3 FRONT VIEW INSIDE

Figure 39
Font view inside

1 Main board 1
2 Waste pump
3 Dilutor motor 3

4 Sample probe vertical motor

5 Sample probe and door hori-
zontal movement machanics
6 Sample probe and door 4

movement motor

5
2

HumaCount 30TS / 60TS | Service manual

Mechanic And Fluidic PARTS 57

8.8.4 SIDE VIEW INSIDE

Figure 40
HC30TS electronics side

1 1 Main board
2 DIMM-PC
2

Figure 41

1 HC30TS pneumatics side

6
1 Valves 1-5
2 7 2 Micro/Sample syringe
8 3 Sampling needle with
washing head
3
4 Measuring chamber
5 Valves 6-10
4
6 Puffer reservoir
7 Macro/Diluent syringe
8 Lyse syringe

5
 58

Figure 42
HC60TS electronics side
1

1 Main board 2

2 DIMM-PC
3 Amplifier board

Figure 43
HC60TS pneumatics side 1

1 Valves 1-5
2 8
2 Micro/Sample syringe
9
3 Sampling needle with 3
washing head
4 10
4 RBC/PLT measuring chamber
5 11
5 WBC/HGB measuring cham-
ber
6 Valves 6-11
7 Puffer reservoir
6
8 Lyse syringe
9 3 diluent syringes
10 Dilutor 1
11 Dilutor 2

HumaCount 30TS / 60TS | Service manual

OPERATION OF THE FLUIDIC SYSTEM 59

9 OPERATION OF THE FLUIDIC SYSTEM

This section describes the main fluidic steps of HC30TS / HC60TS measurement
cycle. The instrument’s fluidic schematics are shown in section „10.5 Tubing
schematics“ on page 81 of this manual. The following figures show total
measurement flow diagram and detailed descriptions of processes for under-
standing the fluidic system work.
The following steps are introduced in this section:

1. Flow diagram of measurement

2. Initialization process
3. Sampling process
4. Needle washing process
5. Diluting process
6. Lysing process
7. Counting process
8. Chamber draining process
9. Shutdown process

In the detailed process description figures, the active tube is filled with black
color, while an arrow shows the direction of the flow. Moving mechanic parts
have another arrow indicating direction of movement. Only opened (On) valves
are mentioned in this section while all the other valves are closed (Off).
HC30TS / HC60TS employs software counters to estimate waste (and other rea-
gent) level. Software integrates volume of the reagents used, and gives a mes-
sage when this volume reaches the preset tank capacity.

9.1 Initialization of the Fluidic System

Fluidic initialization process performs the following steps:
-- Checking of pump and pressure sensor by generating measuring vacuum
-- Positioning all mechanical components by scanning moving range (with
opto sensors)
-- Priming of reagents
-- Cleaning of tubing & measuring chamber
-- Cleaning of aperture with high-pressure back-flush, cleaner reagent &
high-voltage burning
 60

9.2 Operation of the fluidic system

The details of the operation of the instrument can help to identify the subpro-
cess which causes an error.

9.2.1 HC30TS

Figure 44 Previous RBC Diluent of standby

dilution in state in chamber
HC30 flow diagram
TS
chamber (3mL)
Needle washing Draining

Fill up with 1mL

Chamber draining Lysing process diluent for RBC
dilution

Fill up with 1mL Generating

Dilution process
diluent for WBC measuring
(1:256000)
dilution vacuum

WBC counting
Sampling process Needle washing
process

Generating
HGB
Needle washing measuring
measurement
vacuum

Diluting process Extensive RBC

(1:160) cleaning measurement

Sampling process
Blank HGB
(from primary END
measurement
dilution)

HumaCount 30TS / 60TS | Service manual

OPERATION OF THE FLUIDIC SYSTEM 61

9.2.1.1 Sampling process

The aspirating needle aspirates 25 µL (50 µL in prediluted mode) of blood sam-
ple. The Micro dilutor syringe makes the aspirating while the M3 Micro-dilu-
tor motor moves down. The syringes are mechanically connected with a loose
mechanism, so there is a phase along the track, where the micro dilutor doesn’t
move.

V2 DilWash Figure 45

1
V4 DilNeedle

2
1

2 1
V3 DilChamber Sampling process
3

3
2

V5 LyseWbc DILUENT
1 2

3 2 1

Pressure V1 Cleaner
Meter 3
P V9 DilAperture
1 2

CLEANER
Puffer
RBC
Reservoir
WBC
1

Macro Lyse
3

Micro dilutor V8 Bubble

2

Dil M3
HGB
LYSE
2

V7 DrainAperture
M2
1

Ver
2

V6 DrainChamber
2 1
1

V10 DrainPuffer
Sample/
M1 H motor

Pump

2 1 1 2
3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On

There is also another sampling process for the second (RBC) dilution, 25 µL of
primary dilution is aspirated by the sampling needle from the chamber but it
is kept in the sampling needle during the WBC measurement and the cleaning
process.
 62

9.2.1.2 Needle washing process

Both instruments clean the sampling needle with diluent in the washing head
after sampling. It is important to clean the outer surface of the sampling needle
to avoid inaccurate sampling.
The Macro syringe doses and the pump drains the diluent from the washing
head, while sampling needle moves upwards so that the total length of it is
washed and cleaned. This process is called total sampling needle washing, and
it is mainly used after taking primary sample from sample tube.
Another process, which is washing only a smaller part of the sampling needle,
is the same but the needle does not move in the total length. Some procedures
perform this kind of sampling needle washing.
Figure 46
Needle washing V2 DilWash

1
V4 DilNeedle

3
1

2
2 1
V3 DilChamber
3

3
2

V5 LyseWbc DILUENT
1 2

3 2 1

Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2

CLEANER
Puffer
RBC
Reservoir
WBC
1

Macro Lyse
3

Micro dilutor V8 Bubble

2

Dil M3
HGB

LYSE
2

V7 DrainAperture
M2
1

Ver
2

V6 DrainChamber
2 1
1

V10 DrainPuffer Sample/

M1 H motor

Pump

2 1 1 2
3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On

The Macro syringe pushes the diluent through V4 (Off), V3 (Off), V2 (On). The
Pump aspirates the diluent from the washing head through V10(On), while the
M2 Vertical motor moves the sampling needle up.

HumaCount 30TS / 60TS | Service manual

OPERATION OF THE FLUIDIC SYSTEM 63

9.2.1.3 Diluting process

The parts of the fluidics are rinsed with diluent reagent. The measuring chamber
is filled up with 1 ml of diluent. This method prevents the chamber from dirt and
makes the diluting process faster.
The sampling process has aspirated 25 µL of sample, which is in the sampling
needle. In the first diluting step the sample is dispensed into the measuring
chamber with 3 ml of diluent, which comes from the Macro syringe through
V4 (On) and Micro-dilutor, while the M3 Dilutor motor moves upwards. This
process makes the 1:160 first dilution rate in the chamber.

Figure 47
V2 DilWash Diluting process
1

V4 DilNeedle
3

2
1

2 1
V3 DilChamber
3

3
2

V5 LyseWbc DILUENT
1 2

3 2 1

Pressure V1 Cleaner
Meter 3
P V9 DilAperture
1 2

CLEANER
Puffer
RBC
Reservoir
WBC
1

Macro Lyse
3

Micro dilutor V8 Bubble

2

Dil M3
HGB

LYSE
2

V7 DrainAperture
M2
1

Ver
2

V6 DrainChamber
2 1
1

Sample/
V10 DrainPuffer
M1 H motor

Pump

2 1 1 2 3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On

The second sample – 25 µL of primary dilution – is stored in the sampling needle

during the WBC measurement and the cleaning process. The instrument makes
the second (RBC) dilution into the chamber after these processes.
 64

9.2.1.4 Lysing process

In this step the set lysing reagent is added into the measuring chamber through
V5 (On), while the Lyse syringe moves upwards. This process makes the WBC/
HGB dilution with lyse reagent.

Figure 48 V2 DilWash

1
V4 DilNeedle

3
1

2
Lysing process 2 1
V3 DilChamber

3
3

2
V5 LyseWbc DILUENT
1 2

3 2 1

Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2

CLEANER
Puffer
RBC
Reservoir
WBC

1
Macro Lyse
3
Micro dilutor V8 Bubble
Dil M3 2

HGB

LYSE

2
V7 DrainAperture
M2

1
Ver
2

V6 DrainChamber
2 1
1

V10 DrainPuffer Sample/

M1 H motor

Pump

2 1 1 2
3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On

For better mixing the macro syringe pushes some air bubbles (aspirated through
the washing inlet of the chamber and V8) after the lysing process through V4
(Off), V3 (On), V9 (Off) V8 (On).

HumaCount 30TS / 60TS | Service manual

OPERATION OF THE FLUIDIC SYSTEM 65

9.2.1.5 Counting process

The regulated vacuum (it is generated by the pump in the puffer reservoir) aspi-
rates the diluted sample (WBC or RBC) from the chamber through V7 (On) valve.
There is no volume limiter in the system, the instrument counts the cells for
8.5 seconds in both counting phases (WBC and RBC).
Figure 49
V2 DilWash Counting process

1
V4 DilNeedle

3
1

2
2 1
V3 DilChamber
3

3
2

V5 LyseWbc DILUENT
1 2

3 2 1

Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2

CLEANER
Puffer
RBC
Reservoir
WBC
1

Macro Lyse
3

Micro dilutor V8 Bubble

2

Dil M3
HGB

LYSE
2

V7 DrainAperture
M2
1

Ver
2

V6 DrainChamber
2 1
1

V10 DrainPuffer Sample/

M1 H motor

Pump

2 1 1 2
3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On

For noise prevention there is no mechanical or electronic activity during the

counting process and the door should be closed for better shielding.
 66

9.2.1.6 Chamber draining process

Chamber draining is made under pressure control. Pump drains chamber while
puffer reservoir and thus the pressure sensor is connected to the draining tube.
The instrument can detect the empty state of the chamber from drop of vacuum

Figure 50
V2 DilWash

1
Counting process V4 DilNeedle

3
1

2
2 1
V3 DilChamber

3
3

2
V5 LyseWbc DILUENT
1 2

3 2 1

Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2

CLEANER
Puffer
RBC
Reservoir
WBC
1
Macro Lyse
3

Micro dilutor V8 Bubble

2

Dil M3
HGB

LYSE

2
V7 DrainAperture
M2

1
Ver
2

V6 DrainChamber
2 1
1

V10 DrainPuffer Sample/

M1 H motor

Pump

2 1 1 2
3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On

9.2.1.7 Shutdown process

The fluidic shutdown performs the following steps:
-- Priming chamber with reagent to avoid drying out of aperture
-- Sampling needle is positioned above counting chamber, needle up
-- Lyse syringe are positioned up
-- Diluent syringes are positioned up
-- Sample rotor moved out

HumaCount 30TS / 60TS | Service manual

OPERATION OF THE FLUIDIC SYSTEM 67

9.2.2 HC60TS
The HC60TS fluidic system operates in two parallel pneumatic processes. The first
subsystem consists of the following components: Microdilutor, Diluent I. dilutor,
V1 valve, washing head, Pump 2. This subsystem is marked with a dotted line
outline in the charts. The second subsystem consists of the remaining fluidic
components. Thanks to this two parallel subsystems the measuring is started
during the chamber washing processes. The separate RBC and MIX/WBC cham-
bers make two different diluents mixes possible simultaneously.

MIX/WBC Needle
RBC chamber Both chambers
chamber subsystem
Figure 51
Cleaning
Sampling process HC60TS flow diagram
chambers

HGB Blank
measurement

Chamber draining Needle washing

Diluting process Diluting process

(1:160) (1:160)

Sampling process
(from primary
dilution)

Lysing process Needle washing

Dilution process Dilution process

(1 : 23000) (1 : 23000)

Generating
measuring
vacuum

WBC counting
process, HGB
measurement

RBC counting
process

Draining

Cleaning

Fast Blank
measurement

END
 68

9.2.2.1 Sampling process

The aspirating needle aspirates 25 µL (50 µL in prediluted mode) of blood sam-
ple. The Micro dilutor syringe makes the aspirating while the M3 Micro-dilutor
motor moves down. The syringes are mechanically connected with a loose
mechanism, so there is a phase along the track, where the micro dilutor doesn’t
move.

V2 DilNeedle V1 DilWash

1
2 1

3
3

Figure 52

2
1
V4 DilChamber

3
Sampling process

2
P V3 Cleaner
DILUENT
Puffer 2 1

Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3

1 2
CLEANER
Diluent Diluent Lyse V7 Aperture

1
MIX
RBC
V93 WBC

2
Bubble
Diluent

Micro dilutor HGB

M3 M4 M2
LYSE Ver
2

V6 DrainChamber V8 Drainchamber

2
1
1

V11 DrainPuffer 3

1
Sample/
M1 H motor

Pump 1
Pump 2

2 1 1 2
3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE

The second sample – 35 µL of primary dilution – is made in the RBC chamber.

The instrument makes the second (RBC) dilution into the chamber after straight
after the WBC dilution.

HumaCount 30TS / 60TS | Service manual

OPERATION OF THE FLUIDIC SYSTEM 69

9.2.2.2 Diluting process

The parts of the fluidics are rinsed with diluent reagent. The measuring chamber
is filled up with 1 ml of diluent. This method prevents the chamber from dirt and
makes the diluting process faster.
The sampling process has aspirated 25 µL of sample, which is in the sampling
needle. In the first diluting step the sample is dispensed into the measuring
chamber with 3 ml of diluent, which comes from the Diluent I. syringe through
V2 (On) and Micro-dilutor, while the M3 Dilutor motor moves upwards. This pro-
cess makes the 1:160 first dilution rate in the chamber.

2 1
V2 DilNeedle V1 DilWash
1
Figure 53
3

3
2

Diluting process
1

V4 DilChamber
3

P V3 Cleaner
DILUENT
Puffer 2 1

Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3

1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1

MIX
RBC
V9 WBC
3

Bubble
Diluent

Micro dilutor HGB

M3 M4 M2
LYSE Ver
2

V6 DrainChamber V8 Drainchamber
2
1
1

V11 DrainPuffer
1

Sample/
M1 H motor

Pump 1
Pump 2

2 1 1 2
3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE

The second sample – 25 µL of primary dilution – is made right after the first di-
lution. The second dilution is made in the RBC chamber.
 70

9.2.2.3 Lysing process

In this step the set lysing reagent is added into the measuring chamber through
V10 (On), while the Lyse syringe moves upwards. This process makes the WBC/
HGB dilution with lyse reagent.

Figure 54 V2 DilNeedle V1 DilWash

1
2 1

Lysing process

3
3

2
1
V4 DilChamber

2
P V3 Cleaner
DILUENT
Puffer 2 1

Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3

1 2
CLEANER
Diluent Diluent Lyse V7 Aperture

1
MIX
RBC
V9 WBC

2
Bubble
Diluent

Micro dilutor HGB

M3 M4 M2
LYSE Ver
2

2
V6 DrainChamber V8 Drainchamber

2
1
1

3
V11 DrainPuffer

1
Sample/
M1 H motor

Pump 1
Pump 2

2 1 1 2 3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE

For better mixing the macro syringe pushes some air bubbles (aspirated through
the washing inlet of the chamber and V9) after the lysing process through V4
(Off), V10 (On), V9 (Off).

HumaCount 30TS / 60TS | Service manual

OPERATION OF THE FLUIDIC SYSTEM 71

9.2.2.4 Counting process

The regulated vacuum (it is generated by the pump in the puffer reservoir) as-
pirates the diluted sample (WBC or RBC) from the chamber through V11 (On)
valve. There is no volume limiter in the system, the instrument counts the cells
for 16 seconds in counting phases (WBC and RBC).

V2 DilNeedle V1 DilWash Figure 55

1
2 1

3
3

2
Counting process
1

V4 DilChamber
3

P V3 Cleaner
DILUENT
Puffer 2 1

Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3

1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1

MIX
RBC
V9 WBC
3

Bubble
Diluent

Micro dilutor HGB

M3 M4 M2
LYSE Ver
2

V6 DrainChamber V8 Drainchamber
2
1
1

V11 DrainPuffer
1

Sample/
M1 H motor

Pump 1
Pump 2

2 1 1 2
3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE

For noise prevention there is no mechanical or electronic activity during the

counting process and the door should be closed for better shielding.
 72

9.2.2.5 Chamber draining process

Chamber draining is made under pressure control. Pump drains chamber while
puffer reservoir and thus the pressure sensor is connected to the draining tube.
The instrument can detect the empty state of the chamber from drop of vacuum.

Figure 56
V2 DilNeedle V1 DilWash

1
2 1

Chamber draining process

3
3

2
1
V4 DilChamber

2
P V3 Cleaner
DILUENT
Puffer 2 1

Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3

1 2
CLEANER
Diluent Diluent Lyse V7 Aperture

1
MIX
RBC
V9 WBC

2
Bubble
Diluent

Micro dilutor HGB

M3 M4 M2
LYSE Ver
2

2
V6 DrainChamber V8 Drainchamber

2
1
1

3
V11 DrainPuffer

1
Sample/
M1 H motor

Pump 1
Pump 2

2 1 1 2
3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE

HumaCount 30TS / 60TS | Service manual

OPERATION OF THE FLUIDIC SYSTEM 73

9.2.2.6 Cleaning process

The pump aspirates the cleaner through the V3 (On), V10 (On), V7 (On) valves to
puffer the cleaner reagent in the tubes between V10 and V3.

V2 DilNeedle V1 DilWash
Figure 57

1
2 1

3
3

2
Cleaning process
1

V4 DilChamber
3

P V3 Cleaner
DILUENT
Puffer 2 1

Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3

1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1

MIX
RBC
V9 WBC
3

Bubble
Diluent

Micro dilutor HGB

M3 M4 M2
LYSE Ver
2

V6 DrainChamber V8 Drainchamber
2
1
1

V11 DrainPuffer
1

Sample/
M1 H motor

Pump 1
Pump 2

2 1 1 2
3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE

After that the Diluent 2 syringes pushes the cleaner reagent remaining in the
tube between V10 and V3 into the chamber.

9.2.2.7 Shutdown process

The fluidic shutdown performs a fluidic system cleaning with cleaner.
 74

HumaCount 30TS / 60TS | Service manual

Miscellaneous 75

10 MISCELLANEOUS

10.1 Service calibration

The analyzer provides a menu for Service calibration purposes.
You can access the service calibration menu logged in as Service User:
Maintenance-->Calibration-->Service.
Factors
In result calculations the service calibration factors are used as the user calibra-
tion factors, so they are multiplied for each parameter:
RBCDisp. = FactRBC User * FactRBC Serv. * RBCMeasured
If the user factor is near the bound (0.80 - 1.20), by setting the corresponding
service factor, the user factor can be adjusted to 1.00 using Apply user factors
button. Example: Fact RBC User = 1.19 and Fact RBC Serv = 0.96, and Fact RBC
User = 1.00 and Fact RBC Serv = 1.14 gives the same result for RBC.
Press white data field to modify calibration factor. A numeric input screen will
show up so that you can enter values.
All values must be in the 0.8…1.2 range.
Press Accept to proceed with new settings, or Cancel to keep values unchanged.
History
You can check the previous calibration factors with the date of change in a table
form.
Apply user calibration factors
This function is used to combine user and service calibration factors. The soft-
ware will multiply the existing factors, and move them to the Service level to set
user factors to 1.00.

10.2 MDA screen

As part of the system all commands by the software and possible documenta-
tion are displayed on the MDA scrren. This screen appears like the command line
window on usual operating systems. The content looks similar to the following:

Date 2012/02/27
OEM Human GmbH.
Model Humacount 60TS
Serial 920020
Version 2.0
Compiled Dec 16 2011 13:26:58
FPGA firmware 0.14
PIC boot 0.3
 76

10.2.1 EVENT LOG

The content of this screen is like an event log and can be helpful for trouble-
shooting. To store the content of this MDA screen the following steps need to
be performed:
1. Connect an external keyboard
2. Connect a USB flash memory
3. Press Ctrl-Alt-Del on the keyboard. The MDA screen will be shown.
4. Press Ctrl-PrtScr. The information on the MDA screen will be stored as text
file on the USB flash memory.

10.2.2 COMMAND INPUT

Two commands may be entered while MDA screen is active
Print2File: any print command from the software will be directed to a file.
Dontmove: Entering this command will toggle between demo mode and nor-
mal mode.

10.3 USB B connector communication

The byte stream is a human readable ASCII character stream, with occasional
control characters. Most programming environments are able to handle this
stream as a simple ASCII string or text. The stream is line-oriented with special
characters to separate fields. The protocol has a single format for transmitting
a single measurement record. If more records are sent, they are simply chained
together one after the other.

10.3.1 CHARACTERS AND BASIC STRUCTURE

The byte stream uses the ASCII characters in the range 1..255 (http://en.wikipe-
dia.org/Ascii ), or 0x01..0xFF in hexadecimal.
A record transmission consists of three parts: a small header, a big text body, and
a small footer. A single record is never longer than 8192 bytes. A transmission
always starts with the control character „Start of Header” (<SOH>, 1, 0x01). The
second character is a counter: it will contain a single uppercase English letter in
the range „A” to „Z”, incrementing with every record. The first record will con-
tain „A”, the second will contain „B”, etc. If the instrument sends many records
without being turned off, the counter will overflow from „Z” to „A”. The third
character is an identifier: if the instrument is an ABJV5, it will be an uppercase
„A”, and in case of the HC30/60TS it will be an uppercase „N”. The fourth charac-
ter is the control character „Start of Text” (<STX>, 2, 0x02). The fifth and consec-

HumaCount 30TS / 60TS | Service manual

Miscellaneous 77

utive characters form the body of the transmission. The body may contain char-
acters from the printable range (32..126, 0x20..0xFF), and the control characters
„Horizontal tab” (<HT> or <TAB>, 9, 0x09), „Carriage return” (<CR>, 13, 0x0D),
and „Line feed” (<LF>, 10, 0x0A). The body contains several lines separated by
a two-byte sequence <CR><LF>. See below for the detailed description of the
contents. The body of the transmission is closed by the control character „End
of Text” (<ETX>, 3, 0x03).
The footer consists of a two-character checksum in a two-digit hexadecimal
form. The checksum is calculated by summing up the values of all characters in
the message header and body, including the beginning <SOT> character and the
last <ETX> character, adding 255 (hex: 0xFF) to it, and keeping only the last two
hexadecimal(!) digits.
The last character of a record is always the single control character „End of
Transmission” (<EOT>, 4, 0x04). There is no terminating „NULL” (<NUL>, 0, 0x00)
character at the end. The next record can start right after the <EOT> character.

10.3.2 DETAILS OF THE 3.1 PROTOCOL

The body of a transmission is line-oriented, separated by the two-byte „Carriage
Return” „Line Feed” (<CR> <LF>, 13 10, 0x0D 0x0A) sequence. A single line might
contain one or more fields, separated by the „Horizontal tab” (<HT>, 9, 0x09)
character.
The following lines are usually composed of an identifier field and one or more
value fields, all separated by the <HT> character. The characters in bold ap-
pear in the transmission exactly as written, without any variance between re-
cords. Control characters are marked with the < and > characters, for example
<HT>. {Comments} are marked with { and }, and are not included in the actual
transmission. For a more detailed discussion on the meanings of the various
parameters and histograms, please refer to the instruments’ user manuals.

header1 to header 8 header1 to header8 are the lab header lines these lines are
defined by the user in the instrument settings any or all of
these lines can be empty
Serial No.:<HT>serial serial is the 6 digit serial number of the instrument
RecNo:<HT>recno recno is the internal record number, at most 6 digits
Sample ID:<HT>sampleid sampleid is at most 8 characters long
Patient ID:<HT>patientid patientid is at most 20 characters long
Patient Name:<HT>patientname patinetname is at most 32 characters long
Mode:<HT>mode
Doctor:<HT>doctor doctor is at maximum 16 characters long
 78

value is a number of at most 3 digits, unit is either „years”

Age:<HT>value<HT>unit
or „months”
Birth(ymd):<HT>birthdate birthdate is an 8 digit number, format: yyyymmdd
gender is „Male”, „Female”, „Neutered”, „Spayed” or a sin-
Sex:<HT>gender
gle „-” character
Test date(ymd):<HT>date date is an 8 digit number, format: yyyymmdd
Test time(hm):<HT>time time is a 6 digit number, format: hhmmss
Param<HT>Flags<HT>Value<HT>
this is a header line, always the same
Unit<HT>[min-max]
There are 24 similar lines:
param is the parameter name, at most four characters long,
possible values are (in sequence): WBC, RBC, HGB, HCT, MCV,
MCH, MCHC, PLT, PCT, MPV, PDWs, PDWc, RDWs, RDWc,
LYM, MON, NEU, LY%, MO%, NE%, EOS, EO%, BAS, BA%
flag is a single character indicator, can be „ ” (space), „+”, „-”,
„E” and „*”(asterisk) value is the measured parameter value,
param<HT>flag<HT>value<HT>
exactly 4 characters: number with a possible decimal dot,
unit<HT>[min-max]
padded with spaces on the left side, or 4 minus signs „----”,
or 4 spaces „ ” unit is at most 4 characters long, possible
values are „109/L”, „103/µL”, „1012/L”, „106/uL”, „fL”, „%”,
„g/L”, „g/dL”, „mmol/L”, „pg”, „fmol”, depending on the pa-
rameter. Min and max are the lower and upper bounds of
the normal range, exactly 4 characters, including a possible
decimal dot, padded with spaces on the left side
flags is a series of characters indicating errors, at most 32
Flags:<HT>flags
characters long, upper or lowercase letters „a” to „z”
always the same, indicates the beginning of the WBC his-
WBC graph
togram
wbcscale is maximum 3 digit number, indicating the fl val-
Scale(fl):<HT>wbcscale
ue of the last channel, value is usually 400
wbcchannels is the number of channels (columns) in the
Channels:<HT>wbcchannels
histogram, always 256
WMarker1:<HT>wm1 wm1 is the first WBC discriminator channel (RBC/WBC)
WMarker2:<HT>wm2 wm2 is the second WBC discriminator channel (LYM/MON)
WMarker3:<HT>wm3 wm3 is the third WBC discriminator channel (MON/NEU)
chxx is the histogram height at a given channel (range
Points:<HT>ch0<HT>...<HT>ch255 0..255), there are always wbcchannels values here (usually
256)
always the same, indicates the beginning of the RBC histo-
RBC graph
gram
rbcscale is maximum 3 digit number, indicating the fl value
Scale(fl):<HT>rbcscale
of the last channel, value is usually 200
rbcchannels is the number of channels (columns) in the his-
Channels:<HT>rbcchannels
togram, always 256

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Miscellaneous 79

RMarker1:<HT>rm1 rm1 is the RBC discriminator channel (PLT/RBC)

chxx is the histogram height at a given channel (range
Points:<HT>ch0<HT>…<HT>ch255 0..255), there are always rbcchannels values here (usually
256)
always the same, indicates the beginning of the EOS histo-
EOS graph
gram
eosscale is maximum 3 digit number, indicating the fl value
Scale(fl):<HT>eosscale
of the last channel, value is usually 400
eoschannels is the number of channels (columns) in the his-
Channels:<HT>eoschannels
togram, always 256
EMarker1:<HT>em1 em1 is the EOS discriminator channel (WBC/EOS)
chxx is the histogram height at a given channel (range
Points:<HT>ch0<HT>…<HT>ch255 0..255), there are always eoschannels values here (usually
256)
always the same, indicates the beginning of the PLT histo-
PLT graph
gram
pltscale is maximum 3 digit number, indicating the fl value
Scale(fl):<HT>pltscale
of the last channel, value is usually 50
pltchannels is the number of channels (columns) in the his-
Channels:<HT>pltchannels
togram, always 256
PMarker1:<HT>pm1 pm1 is the first PLT discriminator channel (PLT start)
PMarker2:<HT>pm2 pm2 is the second PLT discriminator channel (PLT/RBC)
chxx is the histogram height at a given channel (range
Points:<HT>ch0<HT>…<HT>ch255 0..255), there are always pltchannels values here usually
256)

As mentioned above, after the last channel value in the PLT histogram the body
of the record is closed with the control character „End of Text” (<ETX>, 3, 0x03).
 80

10.4 Cabling diagram

14 13 12 11

Figure 58
Cable connections on Main Board
1

10
1 Horizontal motor (sample 2
9
probe and door)
2 Vertical motor (sample
probe)
3 BLTS board
4 Measure cable to amplifier
board 3

5 DIGIO cable to amplifier

board
6 Dilutor motor
7 Dilutor motor (second
dilutor, HC60TS only)
8 Power switch
9 Dilutor opto sensors (second
dilutor, HC60TS only) 8
4
10 Opto sensors
11 Internal printer
5
12 Waste pump
13 Waste pump (second pump,
HC60TS only)
14 Valves

6 7

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Miscellaneous 81

10.5 Tubing schematics

Figure 59
MAIN Board HC30TS tubing scheme
Pressure
Sensor PUFFER

V1 V2 V3 V4 V5
2 2 2 2 2
3 3 3 3
1 1 1 1 1

M D L

70u

Dilutor

11 1 1 1 1
3 3 3
2 2 2 2 2

V6 V7 V8 V9 V10

out in
Pump
D

W
 82

Figure 60
MAIN Board
HC60TS tubing scheme V1 V2 V3 VV4
12 V5
Pressure
2 2 2 2 2
3
1
3 1 1
3
1
3
1 Sensor

M D1 D2 L
D2
MEA
PUFFER

W W

II.
out in
2 1
3
2 1
3
1
3
1
3
Pump
1 2 1 2 2 2
V6 V7 V6 V9 V10 V11

out I. in
Pump

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Miscellaneous 83

10.6 Recommended kit of tools

-- Screwdrivers:
• Cross Slot Screwdrivers (Philips)
• Slot Screwdrivers
• Hexagon Screwdrivers (3.5, 2.5, 2.0, 1.5 mm sizes)
-- Pocket digital multimeter
-- Diagonal Cutter (plier)
-- Nipper
 84

HumaCount 30TS / 60TS | Service manual

HUMAN
Gesellschaft für Biochemica und Diagnostica mbH
Max-Planck-Ring 21 • 65205 Wiesbaden • Germany
Tel.: +49 6122/9988 0 • Fax: +49 6122/9988 100
eMail: human@human.de • www.human.de