Agilent 6495 Triple Quadrupole LC/MS
with iFunnel Technology
Data Sheet
The Agilent 6495 Triple Quadrupole LC/MS enables the lowest limits of detection and quantitation with performance
specifications in Signal-to-Noise ratio (S/N) and Instrument Detection Limit (IDL). IDL is a rigorous, statistically based metric
that indicates practical sensitivity performance of your quantitative assays. The Agilent 6495 Triple Quadrupole LC/MS
achieves sensitivity and resolution specifications with autotune.
Parameter Measure Specification
MRM sensitivity S/N 1 pg of reserpine injected on column, S/N > 150,000:1
ESI positive quantifying on the transition m/z 609 to 195 Noise 1 x RMS
MRM sensitivity S/N 1 pg of chloramphenicol injected on column, S/N > 150,000:1
ESI negative quantifying on the transition m/z 321 to 152 Noise 1 x RMS
MRM sensitivity IDL 1 fg of reserpine injected on column, IDL < 0.75 fg
ESI positive quantifying on the transition m/z 609 to 195
MRM sensitivity IDL 1 fg of chloramphenicol injected on column, IDL < 0.75 fg
ESI negative quantifying on the transition m/z 321 to 152
Mass range 5 – 2,250 m/z
Polarity switching 30 ms
Mass resolution (autotune) Full width at half maximum 0.7 Da
Mass resolution (manual tune) Full width at half maximum 0.5 Da
Mass accuracy 0.1 Da from 5 – 1,000 m/z
0.2 Da from 1,000 - 2,000 m/z
0.3 Da from 2,000 - 2,250 m/z
Mass stability < 0.1 Da in 24 hours
Dynamic range > 6.0 × 106
Scan modes MS scan, MS/MS product ion scan, MRM, MS/MS neutral loss/gain
scan and precursor ion scan, SIM
Maximum scan rate 15,000 Da/s
Maximum MRM acquisition rate 500 MRMs/sec
Minimum MRM dwell time 1 ms
MRM transitions 450 per time segment, > 40,000 ion transitions per method
Dynamic MRM transitions 4,000 ion transitions per method
Triggered MRM transitions Up to 10 MRM transitions (primary and secondary) for library search
and compound confirmation
Collision cell ion clearance < 1 ms
General system specifications
Parameter Specification
Single point of control Single-point data system method capability with full control of Agilent 1200 Series
HPLC systems and 6495A Triple Quadrupole LC/MS System
Time programming • Polarity change in time segment
• Scan and SIM or MRM (plus other modes of data collection)
• Dynamic and triggered MRM aligns MRMs with compound retention time
• Solvent divert through calibrant delivery system valve
Wide range of ionization sources • Electrospray (ESI)
• Nanospray with HPLC-Chip Cube MS interface
• APCI source (Atmospheric Pressure Chemical Ionization)
• Multimode source (simultaneous ESI and APCI)
• APPI source (Atmospheric Pressure Photo Ionization)
Autotune Automated optimization of ion optics and mass axis calibration in positive and
negative ion modes using a proprietary tune solution
Solvent declustering Countercurrent gas
Detector High-energy conversion dynode and high-gain electron multiplier horn
Vacuum system Two turbomolecular pumps with two mechanical pumps
Ordering information
G6495AA: 6495 Triple Quadrupole LC/MS System
Includes the 6495 Triple Quadrupole Mass Spectrometer, MassHunter Workstation Software with both compliance and method
optimization software, a PC, a monitor and printer, and service installation of the system.
The above are not standard installation specifi cations for the 6495 Triple Quad.
Performance specifi cations in this document are reviewed for accuracy, but they do
not represent the tests and procedures performed at installation, which are described
in the Agilent 6400 Series Triple Quad LC/MS System Installation Manual, document
G3335-90170 or subsequent version number. See Site Preparation Guide and Service
Notes for additional product and specifi cation information.
www.agilent.com/chem/qqq
Agilent Technologies shall not be liable for
errors contained herein or for incidental or
consequential damages in connection with the
furnishing, performance, or use of this material.
Information, descriptions, and specifications in
this publication are subject to change without
notice.
© Agilent Technologies, Inc. 2014
Published in the U.S.A. June 5, 2014
5991-4704EN
