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Food Test

The document describes tests to identify four main biomolecules: glucose, starch, protein, and lipids. For glucose, Benedict's solution is heated with the sample and turns orange or red if glucose is present. Iodine turns blue-black when mixed with starch. Biuret solution turns purple with proteins. And lipids are detected when an ethanol sample emulsion clouds in cold water, indicating a positive test.

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Aisha Ali - 49549/TCHR/EKNNC
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171 views4 pages

Food Test

The document describes tests to identify four main biomolecules: glucose, starch, protein, and lipids. For glucose, Benedict's solution is heated with the sample and turns orange or red if glucose is present. Iodine turns blue-black when mixed with starch. Biuret solution turns purple with proteins. And lipids are detected when an ethanol sample emulsion clouds in cold water, indicating a positive test.

Uploaded by

Aisha Ali - 49549/TCHR/EKNNC
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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1.

Test for glucose (a reducing sugar)

 Add Benedict's solution into sample solution in test tube


 Heat at 60 - 70 °c in water bath for 5 minutes
 Take test tube out of water bath and observe the colour
 A positive test will show a colour change from blue to orange or brick red

The Benedict's test for glucose


2. Test for starch using iodine
We can use iodine to test for the presence or absence of starch in a food sample.

The iodine test for starch

Testing a potato to prove the presence of starch

 Add drops of iodine solution to the food sample


 A positive test will show a colour change from orange-brown to blue-black
3. Test for protein

 Add drops of Biuret solution to the food sample


 A positive test will show a colour change from blue to violet / purple

The Biuret test for protein


4. Test for lipids

 Food sample is mixed with 2cm3 of ethanol and shaken


 The ethanol is added to an equal volume of cold water
 A positive test will show a cloudy emulsion forming

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