Corning® Synthemax® II-SC Substrate
synthetic self-coating substrate for pluripotent stem cell culture
Pluripotency marker expression in hiPSCs during 5 passages
Animal-free, synthetic on Synthemax II-SC Substrate
alternative for feeder layers
and biological coatings
Positive cells (%)
Easy to coat
Matrigel™
Lot traceable Synthemax Surface
Synthemax II-SC Substrate
Gamma irradiated
Quality tested for lot-to-lot
consistency Flow cytometery data
Average of 5 passages +/- STDEV
Cat. # 3535XX1 OCT4 SSEA4
hiPSC doubling time during 7 serial passages on hiPSCs cultured on Synthemax II-SC Substrate for 7
Synthemax II-SC Substrate passages maintain the ability to differentiate to cells of
all three germ layers
Doubling time (hours)
β-III-Tubulin (Ectoderm) α-Actinin (Mesoderm) AFP (Endoderm)
hiPSCs maintain normal karyotype after multiple
Passage number passages on Synthemax II-SC Substrate
OCT4 immunofluorescent staining of hiPSCs after 5
passages on Synthemax II-SC Substrate
Synthemax II-SC Substrate Matrigel™
OCT4
Summary
Synthemax II-SC Substrate coated plates
Hoechst
support multipassage culture of hiPSCs in
defined medium
hiPSCs cultured on Synthemax II-SC Substrate
coated plates maintain pluripotency markers,
normal karyotype and ability to differentiate
Corning and Synthemax are the registered trademarks of Corning to cells of all three germ layers
Incorporated, One Riverfront Plaza, Corning, NY 14831-0001.
All other trademarks are the property of their respective owners
