1. The document describes an experiment to isolate DNA from strawberries. Students mashed strawberries and mixed them with a DNA extraction buffer and cold isopropyl alcohol.
2. A white, fluffy cloud of DNA became visible at the interface between the two liquids. Students were able to isolate DNA fragments and view them under a microscope.
3. The experiment allowed students to learn about the structure of DNA molecules, including the nitrogen bases, phosphate groups, sugars, and the covalent bonds that link them.
1. The document describes an experiment to isolate DNA from strawberries. Students mashed strawberries and mixed them with a DNA extraction buffer and cold isopropyl alcohol.
2. A white, fluffy cloud of DNA became visible at the interface between the two liquids. Students were able to isolate DNA fragments and view them under a microscope.
3. The experiment allowed students to learn about the structure of DNA molecules, including the nitrogen bases, phosphate groups, sugars, and the covalent bonds that link them.
1. The document describes an experiment to isolate DNA from strawberries. Students mashed strawberries and mixed them with a DNA extraction buffer and cold isopropyl alcohol.
2. A white, fluffy cloud of DNA became visible at the interface between the two liquids. Students were able to isolate DNA fragments and view them under a microscope.
3. The experiment allowed students to learn about the structure of DNA molecules, including the nitrogen bases, phosphate groups, sugars, and the covalent bonds that link them.
1. The document describes an experiment to isolate DNA from strawberries. Students mashed strawberries and mixed them with a DNA extraction buffer and cold isopropyl alcohol.
2. A white, fluffy cloud of DNA became visible at the interface between the two liquids. Students were able to isolate DNA fragments and view them under a microscope.
3. The experiment allowed students to learn about the structure of DNA molecules, including the nitrogen bases, phosphate groups, sugars, and the covalent bonds that link them.
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Name: _____________________________ Date Performed: ____________
Year and Course: ____________________ Date Submitted: ____________
Subject title: ________________________ Code number: _______________ Name of Professor: ___________________ Score: ______________________
Exercise no. 8 DNA ISOLATION FROM STRAWBERRIES
I. INTRODUCTION
In 1953, James Waston and Francis Crick proposed a molecular model of
DNA ( Deoxyribonucleic acid) after a year and a half of joint work at Cambridge University. So completely was their model substantiated by subsequent investigations that team shared a Nobel Prince in 1962 with Maurice Wilkins. The basic unit of structure of the DNA molecule is the nucleotide. This is also the basic unit incorporated in DNA during synthesis. The nucleotide consists of three parts: A phosphate, a sugar, and nitrogenous bases.
II. OBJECTIVES: At the end of the experiment, each student will be able to:
1. Isolate DNA fragments from the strawberry.
2. Identify the structural formula of the DNA molecules.
III. MATERIALS 1-3 strawberries (about the volume of a golf ball). Frozen strawberries should be thawed at room temperature. 10 ml DNA Extraction Buffer (soapy salty water) About 20 ml ice cold 91% or 100% isopropyl alcohol 1 Ziploc TM bag, 3- test tubes, test tube brush, test tube holder, test tube rack, 1 Funnel ,1 Coffee stirrer or transfer pipette, 10g baking soda, 2g NaCl Microscope, glass slide, cover slip, triple beam balance, 1-10ml graduated cylinder, 2-water bath, White cloth, toothpick
IV. PROCEDURE
1. Remove the green sepals from the strawberries.
2. Place strawberries into a Ziploc TM bag and seal shut. 3. Squish for a few minutes to completely squash the fruit. 4. Add 10 ml DNA Extraction Buffer (soapy salty water) and squish for a few more minutes. Try not to make a lot of soap bubbles. (buffer must be chilled /place with an ice) Buffer solution: 120ml water, 1.5g NaCl, 5g baking soda 5. Filter through a moistened paper towel set in a funnel, and collect the liquid in a clear test tube. Do not squeeze the paper towel. Collect about 5 ml of liquid. 6. Add the alcohol gently to the top of the solution about an inch and a half above the solution. 7. Add 2 volumes ice cold isopropyl alcohol to the strawberry liquid in the tube. Pour the isopropyl alcohol carefully down the side of the tube so that it forms a separate layer on top of the strawberry liquid. 8. Watch for about a minute. What do you see? You should see a white fluffy cloud at the interface between the two liquids. That’s DNA! 9. Spin and stir the coffee stirrer or transfer pipette in the tangle of DNA, wrapping the DNA around the stirrer. 10. Pull out the stirrer and transfer the DNA to a piece of saran wrap or clean tube. The fibers are thousands and millions of DNA strands. 11. To view in a microscope, put the glob on a clean slide and gently tease/stretch apart using 2 toothpicks or dissecting pins. The fibers will be easier to see the teased-apart area. 12. Rinse your funnel. Put the Ziploc TM bag and white cloth in the garbage.
V. RESULTS AND DISCUSSION
1. Where you able to isolate DNA fragment from your sample? If yes, what is the evidence of your isolates?
Yes, we were able to isolate a DNA fragment from the sample. The white fluffy cloud, DNA, became visible after we mixed the cold isopropyl alcohol, DNA Extraction Buffer (soapy salty water), and the mashed strawberry.
2. Fill out the table below:
Purine bases Pyrimidine bases
Adenine Pair with Thymine Guanine Pair with Cytosine
3. Draw the structural formula of the DNA molecule and properly label its three components: Nitrogen base, phosphate group, and sugar. Determine also which type of bond that link between nitrogen base pairs, sugar and phosphate group.
Phosphate group
Sugar Nitrogen Base
Type of Bond: COVALENT BOND
VI. CONCLUSION
In the end, we were able to isolate the Strawberry DNA fragment by mixing the mashed strawberry with cold isopropyl alcohol and the DNA extraction buffer (water, baking soda, and salt). We were able to identify and draw the structural formula of the DNA through a microscope, and we were also able to label its components and the type of bond that links the components together.
