DNA can be extracted from cells through a basic three step process: 1) lysing the cell and nucleus to release the DNA, 2) precipitating the DNA out of solution using salt and alcohol, and 3) visually observing the precipitated DNA. This document describes extracting DNA from papaya through mechanical grinding to lyse cells and nuclei, using a salt/soap extraction buffer and cold ethanol to precipitate the DNA, which was observed as a cloudy solid mixture separated from the liquid. The results demonstrate the successful extraction and precipitation of DNA from the papaya through this basic process.
DNA can be extracted from cells through a basic three step process: 1) lysing the cell and nucleus to release the DNA, 2) precipitating the DNA out of solution using salt and alcohol, and 3) visually observing the precipitated DNA. This document describes extracting DNA from papaya through mechanical grinding to lyse cells and nuclei, using a salt/soap extraction buffer and cold ethanol to precipitate the DNA, which was observed as a cloudy solid mixture separated from the liquid. The results demonstrate the successful extraction and precipitation of DNA from the papaya through this basic process.
DNA can be extracted from cells through a basic three step process: 1) lysing the cell and nucleus to release the DNA, 2) precipitating the DNA out of solution using salt and alcohol, and 3) visually observing the precipitated DNA. This document describes extracting DNA from papaya through mechanical grinding to lyse cells and nuclei, using a salt/soap extraction buffer and cold ethanol to precipitate the DNA, which was observed as a cloudy solid mixture separated from the liquid. The results demonstrate the successful extraction and precipitation of DNA from the papaya through this basic process.
DNA can be extracted from cells through a basic three step process: 1) lysing the cell and nucleus to release the DNA, 2) precipitating the DNA out of solution using salt and alcohol, and 3) visually observing the precipitated DNA. This document describes extracting DNA from papaya through mechanical grinding to lyse cells and nuclei, using a salt/soap extraction buffer and cold ethanol to precipitate the DNA, which was observed as a cloudy solid mixture separated from the liquid. The results demonstrate the successful extraction and precipitation of DNA from the papaya through this basic process.
SOLUTION OR REAGENT PREPARATIONS: Extraction liquid / lysis liquid
(Salt,water,dish washing liquid.)
PROCEDURE:
1. In a mortar and pestel device put the fruit and mash it to fine pulp
having a goop like consistency. This process breaks down the cell wall
and release the DNA.
2. Prepare extraction/lysis buffer ,in a bowl add about 5g of salt and 3g
of dish washing liquid add water(as required) and gently mix to form
a liquid like consistency.
3. Now add approximately 2 teaspoons of the extraction/lysis buffer
prepared in the previous step to the fruit mixture.
4. Now filter the mixture using a muslin cloth into a beaker.
5. Transfer the liquid from the beaker to a test tube .Now, using a
dropper carefully add the pre frozen ethanol alcohol to the filtered
mixture in the test tube . Roughly 12 drops should do well.
6. Let the test tube rest in a test tube stand for atleast 10 min .
7. As the waiting period gets over there is a cloudy solid coild mixture
formed at the top of the liquid .
8. This cloudy mixture is the precipitated DNA from the fruit-lysis
mixture .
RESULTS AND DISCUSSION
The result of this experiment is that the dna was successfully extracted from the
fruit material . The mechanical method of grinding the fruit helped break down the
cell wall and the extraction buffer consisting of salt and dish washing liquid provided an environment for the dna to be released into the mix and finally the
cold ethanol helped it precipitate into the cloudy floating lumpy form we see in the
picture below separated from the rest of the liquid .
CONCLUSION
The important aspect of this practical is the method that is being used . This
experiment is not like other practicals , as in the results cannot really be evaluated
