This document summarizes a rabbit polyclonal secondary antibody that targets chicken IgY. The antibody is conjugated to horseradish peroxidase (HRP) and can be used for techniques like ICC/IF, dot blot, ELISA, IHC-P, IHC-Fr, immunomicroscopy, and western blot. It is supplied in liquid form with a storage buffer and has been affinity purified and adsorbed to remove unwanted reactivities. Images demonstrate its use for IHC-Fr, ELISA, and western blot applications.
This document summarizes a rabbit polyclonal secondary antibody that targets chicken IgY. The antibody is conjugated to horseradish peroxidase (HRP) and can be used for techniques like ICC/IF, dot blot, ELISA, IHC-P, IHC-Fr, immunomicroscopy, and western blot. It is supplied in liquid form with a storage buffer and has been affinity purified and adsorbed to remove unwanted reactivities. Images demonstrate its use for IHC-Fr, ELISA, and western blot applications.
This document summarizes a rabbit polyclonal secondary antibody that targets chicken IgY. The antibody is conjugated to horseradish peroxidase (HRP) and can be used for techniques like ICC/IF, dot blot, ELISA, IHC-P, IHC-Fr, immunomicroscopy, and western blot. It is supplied in liquid form with a storage buffer and has been affinity purified and adsorbed to remove unwanted reactivities. Images demonstrate its use for IHC-Fr, ELISA, and western blot applications.
This document summarizes a rabbit polyclonal secondary antibody that targets chicken IgY. The antibody is conjugated to horseradish peroxidase (HRP) and can be used for techniques like ICC/IF, dot blot, ELISA, IHC-P, IHC-Fr, immunomicroscopy, and western blot. It is supplied in liquid form with a storage buffer and has been affinity purified and adsorbed to remove unwanted reactivities. Images demonstrate its use for IHC-Fr, ELISA, and western blot applications.
Form Liquid Storage instructions Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Storage buffer Preservative: 0.01% Gentamicin Sulfate (Do not add Sodium Azide!) Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 Purity Immunogen affinity purified Purification notes Horseradish Peroxidase (HRP). Conjugation notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities Clonality Polyclonal Isotype IgG
Applications
ICC/IF ICC/IF: 1/1000 - 1/5000.
Dot Blot Dot: Use at an assay dependent concentration. ELISA ELISA: 1/145000. IHC-P IHC-P: Use at an assay dependent concentration. IHC-Fr IHC-Fr: Use at an assay dependent concentration. Immunomicroscopy IM: Use at an assay dependent concentration. WB WB: 1/2500 - 1/10000.
Images (See the website for higher resolution images of this product)
Ab6753 was used at dilution 1/2000 with the primary antibody ab16293 in IHC-Fr. See the review on ab16293.
Last updated on May
US office: 24, 2012 UK office: Abcam Inc. Abcam plc 330 Cambridge Science Park Cambridge CB4 0FL UK 1Tel:Kendall Square, Suite B2304 Cambridge, MA 02139-1517 USA 888-77-ABCAM (22226) Tel: +44 (0)1223 696000 Fax: 866-739-9884 Fax: +44 (0)1223 771600 These numbers are toll free in the US/Canada Email: orders@abcam.com Email: us.orders@abcam.com Ab6753 was used at dilution 1/10000 with the primary antibody ab15879 in ELISA. See the review on ab15879.
Ab6753 was used at dilution 1/5000 with the primary antibody ab19130 in WB. See the review on ab19130.
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Last updated on May
US office: 24, 2012 UK office: Abcam Inc. Abcam plc 330 Cambridge Science Park Cambridge CB4 0FL UK 1Tel:Kendall Square, Suite B2304 Cambridge, MA 02139-1517 USA 888-77-ABCAM (22226) Tel: +44 (0)1223 696000 Fax: 866-739-9884 Fax: +44 (0)1223 771600 These numbers are toll free in the US/Canada Email: orders@abcam.com Email: us.orders@abcam.com
