Intern at ional Journal of Applied Research 20 15; 1(12): 105-107

ISSN Print: 2394-7500

ISSN Online: 2394-5869
Impact Factor: 5.2 Assessment of total phenolic, flavonoid content and
IJAR 2015; 1(12): 105-107
www.allresearchjournal.com
Anti-oxidant potential of Peltophorum pterocarpum
Received: 18-09-2015
Accepted: 19-10-2015
(DC.) Baker ex. K. Heyne flower extracts
B Amala
P.G and Research Department B Amala, TV Poonguzhali
of Botany, Queen Mary’s
College, Chennai, India.
Abstract
The objective of the present study was to evaluate photochemical screening, and estimate total phenol,
TV Poonguzhali
P.G and Research Department total flavonoid, and antioxidant potential of the flowers extract of Peltophorum pterocarpum by DPPH
of Botany, Queen Mary’s radical scavenging assay. Total phenolic and flavonoid contents were evaluated according to Folin-
College, Chennai, India. Ciocalteau procedure and a calorimetric method, respectively. It showed high content of total phenolic
compounds and total flavonoids. In vitro antioxidant activity of petroleum ether, chloroform, acetone,
aqueous and ethanol extracts was evaluated by studying 1, 1-diphenyl-2-picrylhydrazyl radical
scavenging activity using the standard procedure. The result shows that acetone extract of P.
pterocarpum flowers had highest antioxidant activity (93.5%) using DPPH method.

Keywords: Peltophorum pterocarpum, Antioxidant, DPPH.

Introduction
The medicinal properties of plants have been investigated in the recent scientific
developments throughout the world, due to their potential antioxidant activities, no side
effects and economic viability [1]. Recently there has been an upsurge of interest in the
therapeutic potentials of medicinal plants as antioxidants in reducing such free radical
induced tissue injury.
Antioxidants are play a very important key role in the body defense mechanism against
reactive oxygen species (ROS), which are the harmful by products generated during normal
cell aerobic respiration [2]. Many recent research have proved that antioxidants are radical
scavengers, which protect the human body against free radicals that may cause pathological
conditions such as ischemia, anaemia, asthma, arthritis, inflammation, neurodegeneration,
and ageing process [3]. Many experimental datas suggests that free radical and reactive
oxygen species can be involved in several number of diseases [4, 5]. As plants produces a lot
of antioxidants to control the oxidative stress caused by sunbeams and oxygen, they can
represent a source of compounds with antioxidant activity.
The phenolic compounds are considered as one of the largest and most ubiquitous groups of
plant metabolites [6]. Plant phenolics are commonly found in both edible and non-edible
plants, and have various biological effects, including antioxidant activity. The antioxidant
activity of phenolics is mainly due to their redox properties, which allow to act as reducing
agents, hydrogen donators, and singlet oxygen quenchers. In addition, they have a metal
chelation potential. Recent studies have focused on the biological activities of phenolic
compounds, which are antioxidants and free radical scavengers [7-9]. The extracts of more
numbers of herbs, spices and other plant materials rich in phenolics and flavonoids are of
increasing interest in the food industry because they retard oxidative degradation of lipids
and there by improving nutritional value of food [10].

Correspondence Materials and Methods

B Amala Fresh flowers of P. pterocarpum were collected from different places of Chennai. The leaves
P.G and Research Department were washed thoroughly with normal tap water followed by sterile distilled water. Then the
of Botany, Queen Mary’s leaves were shade dried at room temperature. These were crushed to powder using grinding
College, Chennai, India.
machine. The powdered sample was analysed for qualitative inorganic compounds.
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[17]
Preparation of extracts . Subsequently, at every 5 minutes interval, the absorption
Preparation of the extracts was following the standard maxima of the solution were measured using a UV double
methods [11, 12]. About 15g of fine dried powdered flowers of beam spectra scan (Chemito, India) at 517 nm. The
P. pterocarpum were extracted with 150mL of ethanol antioxidant activity of the sample was compared with known
(75%), acetone Chloroform, petroleum ether and aqueous synthetic standard of (0.16%) of butylated hydroxy toluene
extract for 1 min using an Ultra Turax mixer (13,000rpm) (BHT). The experiment was carried out in triplicates.
and soaked overnight at room temperature. The samples was The capacity of scavenging free radicals activity was
then filtered through Whatman No.1 paper in Buchner calculated by the formula
funnel. The filtered solution was evaporated under vaccum in
a rota-evator at 40 ºC and then dissolved in respective Scavenging activity (%) = (A of Control-A of Sample)/A of
solvents. The concentrated extracts were stored in airtight Control ×100
container in refrigerator below 10 ºC.
Result and Discussion
Estimation of Total Phenol Content in Flowers Extracts
of P. pterocarpum Table 1: Estimation of total phenol and flavanoid content of
Total phenolic content in the flowers extracts was flowers extract of Peltophorum pterocarpum
determined by the Folin–Ciocalteau colorimetric method [13]. Total phenol
For the analysis, 0.5 mL of aliquot of sample was added to Total flavanoid
Sample content (mg
0.5 mL of Folin–Ciocalteau reagent (0.5 N) and the contents content (mg /g)
GAE/g)
of the flask were mixed thoroughly. Later 2.5 mL of sodium Peltophorum
carbonate (2%) was added, and the mixture was allowed to pterocarpum flowers 80 48.7
stand for 30 minutes after mixing. The absorbance was extract
measured at 760 nm in a UV-Visible Spectrophotometer. The GAE: Gallic acid equivalents
total phenolics contents were expressed as mg gallic acid
equivalents (GAE)/g extract. Table2: DPPH scavenging activity of flowers extract of
Peltophorum pterocarpum
Estimation of Total Flavanoid Content in Flowers Flower extracts of P. pterocarpum % of inhibition for 100 µl
Extracts of P. pterocarpum Petroleum ether 84
Total flavonoids content of P. pterocarpum was determined Chloroform 84.6
by the aluminium chloride colorimetric method [14]. 0.5 mL Acetone 93.5
of flower extracts of P. pterocarpum at a concentration of Ethanol 91.3
1mg/ mL were taken and the volume was made up to 3mL Aqueous 92
with methanol. Then 0.1mL AlCl3 (10%), 0.1mL of BHT (Standard) 98.6
potassium acetate and 2.8 mL distilled water were added
sequentially. The test solution was vigorously shaken.
Absorbance was recorded at 415 nm after 30 minutes of
incubation. A standard calibration plot was generated at
415nm using known concentrations of quercetin. The
concentrations of flavonoid in the test samples were
calculated from the calibration plot and expressed as mg
quercetin equivalent /g of sample.

Qualitative Analysis of Antioxidant Activity of P.

pterocarpum flowers
The antioxidant activity of flower extracts of P. pterocarpum
was determined by standard method [15, 16]. 50 μL of flower
extracts of P. pterocarpum were taken in the microtiter plate.
100 μL of 0.1% methanolic 1, 1-diphenyl-2-picrylhydrazyl
(DPPH) was added over the samples and incubated for Fig 1: DPPH scavenging activity of flowers extract of Peltophorum
pterocarpum
30 minutes in dark condition. The samples were then
observed for discoloration, from purple to yellow and pale
Discussion
pink were considered to be strong and weak positive
The present study was to investigate the antioxidant activity
respectively. The antioxidant positive samples were
and phenolic contents of P. pterocarpum flowers. In the
subjected for further quantitative analysis.
present paper, we have evaluated the free radical scavenging
activity of ethanol, acetone, pet. ether, chloroform and
Quantitative Analysis of Free Radical Scavenging
aqueous extracts of P. pterocarpum flowers. The antioxidant
Activity of P. Pterocarpum flowers
properties of P. pterocarpum have been evaluated by DPPH
The antioxidant activities were determined using DPPH,
method. Scavenging activity for free radicals of 1.1-
(Sigma-Aldrich) as a free radical. Flowers extract of 100 μL
diphenyl-2- picrylhydrazyl (DPPH) has been widely used to
were mixed with 2.7 mL of methanol and then 200 μL of
evaluate the antioxidant activity of natural products from
0.1% methanolic DPPH was added. The suspension was
plant and microbial sources [18]. Among the five different
incubated for 30 minutes in dark condition. Initially,
solvents extracts tested, the acetone extract showed better
absorption of blank containing the same amount of methanol
DPPH scavenging activity. A maximum scavenging activity
and DPPH solution was prepared and measured as a control
was offered by acetone extract of P. pterocarpum (93.5 %)
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and followed by Aqueous (92%), ethanol (91.3%), chilensis [Elaeocarpaceae], Maqui. Food Chem 2008;
chloroform (84.6%) and pet.ether (84%). 107(2):820-9.
Polyphenolic compounds are commonly found in both edible 9. Reddy BS, Reddy BP, Raghavulu SV, Ramakrishna S,
and non-edible plants, they have multiple applications in Venkateswarlu Y, Diwan PV. Evaluation of antioxidant
food, cosmetic and pharmaceutical industries [19]. The and antimicrobial properties of soymida febrifuga leaf
concentration of phenols in the examined flowers extracts extracts. Phytother Res 2008; 22(7):943-7.
using the Folin-Ciocalteu reagent was expressed in terms of 10. Chu YH, Chang CL, Hsu HF. Flavonoid content of
gallic acid equivalent. several vegetables and their antioxidant activity. J Sci
Table 1 shows the estimation of total phenol and flavonoid Food Agric 2000; 80(5):561-6.
content in the flowers extract of P. pterocarpum. The higher 11. Pizzale L, Bortolomeazzi R, Vichi S, Conte LS.
content of total phenol (80 mg GAE/g) and total flavonoid Antioxidant activity of sage and oregano extracts related
(48.7g) was found in flowers extract of P. pterocarpum. It to their phenolic compound content. J Sci Food Agric.
has been recognized that flavonoids show antioxidant 2002; 82(14):1645-51.
activity and their effects on human nutrition and health are 12. Lu Y, Foo Y. Antioxidant activities of polyphenols from
considerable. The mechanisms of action of flavonoids are sage (Salvia officinalis). Food Chem 2001; 75(2):197-
through scavenging or chelating process [20]. Phenolic 202.
compounds are a class of antioxidant agents which act as free 13. Slinkard K, Singleton VL. Total phenol analysis:
radical terminators [21]. The value of phenolic content Automation and comparison with manual Methods. Am
indicates that the plant has antioxidant activity. Obviously, to J Enol Vitic. 1977; 28(1):49-55.
confirm the beneficial effects of these extracts, it is necessary 14. Winky DR, Salatino A. Analysis of propolis some
to carry out further studies about their in vivo activity and parameter and procedures forchemical quality control. J
bioavailability. Apic Res. 1998; 37-99-105.
15. Hsiao G, Teng CM, Wu CL, Ko FN. Marchantin Has a
Conclusion natural antioxidant and free radical scavenger. Arch
The present study revealed a marked antioxidant potential of Biochem Biophys 1996; 334(1):18-26.
flowers extracts. The flowers can be used as a remedy for 16. Abirami MS, Muthuswamy. Antioxidant potential, total
treatment of oxidative damage caused by free radicals. The phenolic and total flavonoids content of various extracts
antioxidant activity of flowers extracts might be attributed to from whole plant of Polycarpaea corymbosa lam. Asian
the presence of phenolic compounds and flavonoids. The J Pharm Clin Res. 2013; 6(4):121-4.
strong antioxidant properties were confirmed in the acetone 17. Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH.
flowers extract. The result of the present study appear as Screening of medicinal plant extracts for antioxidant
interesting and promising in the search of potent antioxidant activity. Life Sci 2003; 73(2):167-79.
agent and may be effective as potential source novel 18. Lie-Fen Shyura, Jieh-Hen Tsunga, Je-Hsin Chenb, Chih-
antioxidant drugs. Yang Chiua, Chiu-Ping Lo. Antioxidant Properties of
Extracts from Medicinal Plants Popularly Used in
References Taiwan. International Journal of Applied Science and
1. Auudy B, Ferreira F, Blasina L, Lafon F, Arredondo F, Eng. 2005; 3(3):195-202.
Dajas R et al. Screening of antioxidant activity of three 19. Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja
Indian medicinal plants, traditionally used for the K, Kujala TS. Antioxidant activity of plant extracts
management of neurodegenerative diseases. J containing phenolic compounds. J Agri Food Chem.
Ethnopharmacol. 2003; 84:131-138(s). 1999; 47:3954-3962.
2. Gutteridge JMC, Halliwell B. Free radicals and 20. Kessler M, Ubeaud G, Jung L. Anti-and pro-oxidant
antioxidants in the year 2000 – A historical look to the activity of rutin and quercetin derivatives. J Pharm
future. Ann. N. Y. Acad. Sci 2000; 899:136-147. Pharmacol. 2003; 55:131-142.
3. Ames BN, Shigenaga MK, Hagen TM. Oxidants, 21. Om Prakash T, Yamini BT. Antioxidant properties of
antioxidants and the generative disease of aging. Proc different fractions of Vitex negundo Linn. Food Chem
Natl Acad Sci 1993; 90:7915-7922. 2007; 100(3):1170-1176.
4. Richards RT, Sharma HM. Free radicals in health and
disease, Indian J Clin Pract. 1991; 1:15-26.
5. Niwa Y. Effect of Maharishi 4 and Maharish 5 on
inflammatory mediators with special reference to their
free radical scavenging effect, J Clin Pract. 1991; 1:23-
27.
6. Hagerman AN, Rield KM, Jones GA, Sovik KN,
Ritchard NT, Hartzfeld PW et al. High molecular weight
plat polyphenolics (tannins) as biological antioxidants. J
Agric Food Chem. 1998; 46:1887-92.
7. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM,
Pridham JB. The relative antioxidant activities of plant-
derived polyphenolic flavonoids. Free Radic Res 1995;
22(4):375-83.
8. Cespedes CL, El-Hafidi M, Pavon N, Alarcon J.
Antioxidant and cardioprotective activities of phenolic
extracts from fruits of Chilean blackberry Aristotelia
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