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Lissamine, Schmorl, Ground, Sudan Method

The document describes several histopathology staining techniques used to visualize different tissue components under a microscope. The Lissamine Fast Red Tartrazine stain uses formol sublimate fixation and stains muscle and reticulin fibers red, nuclei black, and collagen yellow. Schmorl's picro-thionin method stains bone lacunae and canaliculi dark brown using thionin and picric acid. Ground section preparation of bones analyzes haversian canals in thin ground bone sections after formalin fixation. The Sudan Black method stains lipids blue-black in unfixed or frozen cryostat sections.

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251 views3 pages

Lissamine, Schmorl, Ground, Sudan Method

The document describes several histopathology staining techniques used to visualize different tissue components under a microscope. The Lissamine Fast Red Tartrazine stain uses formol sublimate fixation and stains muscle and reticulin fibers red, nuclei black, and collagen yellow. Schmorl's picro-thionin method stains bone lacunae and canaliculi dark brown using thionin and picric acid. Ground section preparation of bones analyzes haversian canals in thin ground bone sections after formalin fixation. The Sudan Black method stains lipids blue-black in unfixed or frozen cryostat sections.

Uploaded by

ryan caballerobatuang
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Stain Purpose Fixation Result Section

Muscle striations are well shown Formol Sublimate  Nuclei = Black Thin Paraffin
 Muscle and RBS = Red Section
 Collagen = Yellow

Lissamine Test - Red Tartrazine


Method

Schmorl’s picro-thionin Any fixative but avoid  Lacuni and canaliculi Frozen or celloidin
precipitates within the bone mercuric chloride has a dark brown black freshly cut
canaliculi accelerated by 0.125 % stain;
thionin. This stain the compact  bone matrix yellow or
bone employs thionin and picric brown yellow and
acid to produce color in the  cells are red
canaliculi

Schmorl’s Picro-Thionin Method


(1934)
Ground Bone sections are 10 % Formalin  Lacunae and canaliculi Thin ground
analyzed to determine the number filled with air lamellae sections
of and width of haversian canals brown or brownish black
line

Ground Section Preparation of Bones

Sudan dyes are lipid soluble lipids Formaldehyde calcium  Lipids are blue black Unfixed cryostat
therefor can penetrate the with post- chorming  Nuclei is red sections preferred,
specimen. Sudan Black is slightly or frozen sections
basic dye and will combine with post fixed in formol
acidic groups in compound lipids, calcium
thus staining phospholipid also.

Sudan Black Method for Lipids

References
Gregorios, Jocelyn et al, 2006, Histopathologic Techniques 2 nd Edition, Retrieved from https://books.google.com.ph/books?
id=DCMUb2chHzUC&pg=PA225&lpg=PA225&dq=lissamine+fast+red+tartrazine+muscle+bone&source=bl&ots=sfEdApN5ax&sig=ACfU3U0ifjLk6H1IgASC4Ifn1H3EREGBlg&hl
=en&sa=X&ved=2ahUKEwi63qWY4YLpAhUawosBHQrOBTEQ6AEwAHoECAoQAQ#v=onepage&q=lissamine%20fast%20red%20tartrazine%20muscle%20bone&f=false
Gregorios, Jocelyn and Faldas, Marc, 2017, Histopathologic Techniques, pgs 339, 556-557
Dettmeyer, Reinhard, 2011, Forensics Histopathology; Fundamentals and Perspective, Retrieved from https://books.google.com.ph/books?
id=PVY45hQYJZwC&pg=PA231&dq=ground+section+for+bones+histopathology&hl=en&sa=X&ved=0ahUKEwi7qfWg6oLpAhUTfXAKHbYmCcIQ6AEISjAE#v=onepage&q=grou
nd%20section%20for%20bones%20histopathology&f=false

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