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in

Organic qualitative analysis - 1 (1.benzaldehyde)

S.no Experiment Observation Inference

Preliminary tests

1 Odour:
No characteristic odour Absence of amine,phenol ann
Note the Odour of the organic compound. benzaldehyde

2 Reaction with litmus paper:

No colour change is noted Absence of carboxylic acid ,
Touch the Moist litmus paper with an organic phenol and amine
compound.

3 Action with sodium bicarbonate:

in
No brisk effervescence is Absence of a carboxylic acid.
Take 2 ml of saturated sodium bi carbonate solution obtained
in a test tube. Add 2 or 3 drops (or a pinch of solid)

s.
of an organic compound to it.

s
4 Action with Borsche’s reagent:

re
Take a small amount of an organic compound in a yellow or orange or red Presence of an aldehyde or
test tube. Add 3 ml of Borsche’s reagent, 1 ml of precipitate ketone
Conc HCl to it, then warm the mixture gently and
xp
cool it.
e
5 Charring test:
vi

Take a small amount of an organic compound in a dry No Charring takes place absence of carbohydrate
test tube. Add 2 ml of conc H2SO4 to it, and heat the with smell of burnt sugar
mixture.
al

Tests for Aliphatic or Aromatic nature:

.k

6 Ignition test:
Burns with sooty flame Presence of an aromatic
w

Take small amount of the organic compound in a compound

Nickel spatula and burn it in Bunsen flame.
w

Tests for an unsaturation:

w

7 Test with bromine water:

No Decolourisation takes Substance is saturated.
Take small amount of the organic compound in a place
test tube add 2 ml of distilled water to dissolve it. To
this solution add few drops of bromine water and
shake it well.

8 Test with KMnO4 solution:

No Decolourisation takes Substance is saturated.
Take small amount of the organic compound in a place
test tube add 2 ml of distilled water to dissolve it. To
this solution add few drops of very dilute alkaline

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KmnO4 solution and shake it well.

TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

Test For Phenol

9 Neutral FeCl3 test:

No violet or blue colouration is abesence of phenol.
Take 1 ml of neutral ferric chloride solution is taken seen
in a dry clean test tube. Add 2 or 3 drops (or a pinch
of solid) oforganic compound to it. If no colouration
occurs add 3 or 4 drops of alcohol.

TEST FOR CARBOXYLIC ACIDS

in
10 Esterification reaction:
No Pleasant fruity odour is noted. absence of carboxylic
Take 1 ml (or a pinch of solid) of an organic group.

s.
compoundin a clean test tube. Add 1 ml of ethyl
alcohol and 4 to 5 drops of conc. sulphuric acid to

s
it. Heat the reaction mixture strongly for about 5
minutes. Then pour the mixture into a beaker

re
containing dil. Sodium carbonate solution and note
the smell.
Test for aldehydes.
xp
11 Tollen’s reagent test:
Shining silver mirror is formed. Presence of an aldehyde
Take 2 ml of Tollen’s reagent in a clean dry test
tube. Add 3-4 drops of an organic compound (or
e
0.2 g of solid) to it, and warm the mixture on a
vi

water bath for about 5 minutes.

12 Fehling’s test:
Red precipitate is formed. Presence of an aldehyde
al

Take 1 ml each of Fehling’s solution A and B are

taken in a test tube. Add 4-5 drops of an organic
compound (or 0.2g of solid) to it, and warm the
.k

mixture on a water bath for about 5 minutes.

Test for ketones
w

13 Legal’s test:
No Red colouration. absence of a ketone.
A small amount of the substance is taken in a test
w

tube. 1 ml sodium nitro prusside solution is added.

Then sodium hydroxide solution is added
w

dropwise.
Test for an amine.
14 Dye test:
Take A small amount of an organic substance in a Scarlet red dye is not obtained. absence of an aromatic
clean test tube, add 2 ml of HCl to dissolve it. Add primary amine
few crystals of NaNO2, and cool the mixture in ice
bath. Then add 2 ml ofice cold solution of β-
naphtholin NaOH.

Test for diamide

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15 Biuret test:
Take A small amount of an organic compound in a test No Violet colour is appeared. absence of a diamide
tube. Heat strongly and then allow to cool. Dissolve the
residue with 2 ml of water. To this solution Add 1 ml of
dilute copper sulphate solution and few drops of 10%
NaOH solution drop by drop.

Test for carbohydrates

16 Molisch’s test:
Take A small amount of an organic compound in a test No Violet or purple ring is absence of carbohydrate
tube. It is dissolved in 2 ml of water. Add 3-4 drops of formed at the junction of the
alpha naphtholto it.Then add conc H2SO4 through the two liquids.
sides of test tube carefully.

17 Osazone test:

in
Take A small amount of an organic compound in a test Yellow crystals are not absence of carbohydrate
tube. Add 1 ml of phenyl hydrazine solution and heat the obtained

s.
mixture for about 5 minutes on a boiling water bath.

s
Result:

re
The given organic compound contains /is

(i) Aromatic
xp
(ii) Saturated
(iii) aldehyde functional group ( benzaldehyde )
e
vi
al
.k
w
w
w

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Organic qualitative analysis – 2 (2.cinnamaldehyde)

S.no Experiment Observation Inference

Preliminary tests

1 Odour:
No characteristic odour Absence of amine,phenol ann
Note the Odour of the organic compound. benzaldehyde

2 Reaction with litmus paper:

No colour change is noted Absence of carboxylic acid ,
Touch the Moist litmus paper with an organic phenol and amine
compound.

in
3 Action with sodium bicarbonate:
No brisk effervescence is Absence of a carboxylic acid.

s.
Take 2 ml of saturated sodium bi carbonate solution obtained
in a test tube. Add 2 or 3 drops (or a pinch of solid)

s
of an organic compound to it.

re
4 Action with Borsche’s reagent:
Take a small amount of an organic compound in a
xp yellow or orange or red Presence of an aldehyde or
test tube. Add 3 ml of Borsche’s reagent, 1 ml of precipitate ketone
Conc HCl to it, then warm the mixture gently and
e
cool it.
vi

5 Charring test:
al

Take a small amount of an organic compound in a No Charring takes place with absence of carbohydrate
dry test tube. Add 2 ml of conc H2SO4 to it, and heat smell of burnt sugar
the mixture.
.k

Tests for Aliphatic or Aromatic nature:

w

6 Ignition test:
Burns with sooty flame Presence of an aromatic
w

Take small amount of the organic compound in a compound

Nickel spatula and burn it in Bunsen flame.
w

Tests for an unsaturation:

7 Test with bromine water:

Decolourisation takes place Substance is unsaturated.
Take small amount of the organic compound in a test
tube add 2 ml of distilled water to dissolve it. To this
solution add few drops of bromine water and shake it
well.

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8 Test with KMnO4 solution:

Decolourisation takes place Substance is unsaturated.
Take small amount of the organic compound in a test
tube add 2 ml of distilled water to dissolve it. To this
solution add few drops of very dilute alkaline KmnO4
solution and shake it well.

TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

Test For Phenol

9 Neutral FeCl3 test:

No violet or blue abesence of phenol.
Take 1 ml of neutral ferric chloride solution is taken in colouration is seen
a dry clean test tube. Add 2 or 3 drops (or a pinch of
solid) oforganic compound to it. If no colouration
occurs add 3 or 4 drops of alcohol.

in
TEST FOR CARBOXYLIC ACIDS

s.
10 Esterification reaction:
No Pleasant fruity odour is absence of carboxylic

s
Take 1 ml (or a pinch of solid) of an organic noted. group.
compoundin a clean test tube. Add 1 ml of ethyl alcohol

re
and 4 to 5 drops of conc. sulphuric acid to it. Heat the
reaction mixture strongly for about 5 minutes. Then
pour the mixture into a beaker containing dil. Sodium
carbonate solution and note the smell.
xp
Test for aldehydes.
e
11 Tollen’s reagent test:
Shining silver mirror is formed. Presence of an aldehyde
vi

Take 2 ml of Tollen’s reagent in a clean dry test tube.

Add 3-4 drops of an organic compound (or 0.2 g of
al

solid) to it, and warm the mixture on a water bath for

about 5 minutes.
Fehling’s test:
.k

12
Red precipitate is formed. Presence of an aldehyde
Take 1 ml each of Fehling’s solution A and B are taken
in a test tube. Add 4-5 drops of an organic compound
w

(or 0.2g of solid) to it, and warm the mixture on a water

bath for about 5 minutes.
w

Test for ketones

13 Legal’s test:
w

No Red colouration. absence of a ketone.

A small amount of the substance is taken in a test tube.
1 ml sodium nitro prusside solution is added. Then
sodium hydroxide solution is added dropwise.

Test for an amine.

14 Dye test:
Take A small amount of an organic substance in a clean Scarlet red dye is not obtained. absence of an aromatic
test tube, add 2 ml of HCl to dissolve it. Add few primary amine
crystals of NaNO2, and cool the mixture in ice bath.
Then add 2 ml ofice cold solution of β-naphtholin
NaOH.

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Test for diamide

15 Biuret test:
Take A small amount of an organic compound in a No Violet colour is appeared. absence of a diamide
test tube. Heat strongly and then allow to cool.
Dissolve the residue with 2 ml of water. To this
solution Add 1 ml of dilute copper sulphate solution
and few drops of 10% NaOH solution drop by drop.

Test for carbohydrates

16 Molisch’s test:
Take A small amount of an organic compound in a No Violet or purple ring is absence of carbohydrate
test tube. It is dissolved in 2 ml of water. Add 3-4 formed at the junction of the
drops of alpha naphtholto it.Then add conc H2SO4 two liquids.
through the sides of test tube carefully.

in
17 Osazone test:

s.
Take A small amount of an organic compound in a Yellow crystals are not absence of carbohydrate
test tube. Add 1 ml of phenyl hydrazine solution and obtained
heat the mixture for about 5 minutes on a boiling

s
water bath.

re
Result:
xp
The given organic compound contains /is
e
(i) Aromatic
(ii) unsaturated
vi

(iii) aldehyde functional group (cinnamaldehyde )

al
.k
w
w
w

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Organic qualitative analysis -3 (3.acetophenone)

S.no Experiment Observation Inference

Preliminary tests

1 Odour:
No characteristics odour Absence of
Note the Odour of the organic compound. amine,phenol,ester and
benzaldehyde

2 Reaction with litmus paper:

No colour change is noted Absence of carboxylic
Touch the Moist litmus paper with an organic acid , phenol and amine
compound.

3 Action with sodium bicarbonate:

in
No brisk effervescence is obtained Absence of a carboxylic
Take 2 ml of saturated sodium bi carbonate acid.
solution in a test tube. Add 2 or 3 drops (or a

s.
pinch of solid) of an organic compound to it.

s
4 Action with Borsche’s reagent:

re
Take a small amount of an organic compound in yellow or orange or red precipitate Presence of an aldehyde or
a test tube. Add 3 ml of Borsche’s reagent, 1 ml ketone
of Conc HCl to it, then warm the mixture gently
and cool it.
xp
5 Charring test: Charring does not takes place with Absence of carbohydrate
smell of burnt sugar
e
Take a small amount of an organic compound in a
dry test tube. Add 2 ml of conc H2SO4 to it, and
vi

heat the mixture.

al

Tests for Aliphatic or Aromatic nature:

6 Ignition test:
.k

Burns with not-sooty flame Presence of an aliphatic

Take small amount of the organic compound in a compound
Nickel spatula and burn it in Bunsen flame.
w

Tests for an unsaturation:

w

7 Test with bromine water:

w

Take small amount of the organic compound in a No Decolourisation takes place Substance is saturated.
test tube add 2 ml of distilled water to dissolve it.
To this solution add few drops of bromine water
and shake it well.

8 Test with KMnO4 solution:

Take small amount of the organic compound in a No Decolourisation takes place Substance is saturated.
test tube add 2 ml of distilled water to dissolve it.
To this solution add few drops of very dilute
alkaline KmnO4 solution and shake it well.

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TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

Test For Phenol

9 Neutral FeCl3 test:

No Violet or blue absence of phenolic compound
Take 1 ml of neutral ferric chloride solution is taken in colouration is seen
a dry clean test tube. Add 2 or 3 drops (or a pinch of
solid) oforganic compound to it. If no colouration
occurs add 3 or 4 drops of alcohol.

TEST FOR CARBOXYLIC ACIDS

10 Esterification reaction: No Pleasant fruity odour is abesence of carboxylic group.

noted.
Take 1 ml (or a pinch of solid) of an organic
compoundin a clean test tube. Add 1 ml of ethyl
alcohol and 4 to 5 drops of conc. sulphuric acid to it.
Heat the reaction mixture strongly for about 5

in
minutes. Then pour the mixture into a beaker
containing dil. Sodium carbonate solution and note

s.
the smell.

Test for aldehydes.

s
11 Tollen’s reagent test: No Shining silver mirror is absence of an aldehyde
formed.

re
Take 2 ml of Tollen’s reagent in a clean dry test tube.
Add 3-4 drops of an organic compound (or 0.2 g of
solid) to it, and warm the mixture on a water bath for
xp
about 5 minutes.
12 Fehling’s test: No Red precipitate is absence of an aldehyde
formed.
Take 1 ml each of Fehling’s solution A and B are taken
e
in a test tube. Add 4-5 drops of an organic compound
vi

(or 0.2g of solid) to it, and warm the mixture on a

water bath for about 5 minutes.
al

Test for ketones

13 Legal’s test: Red colouration. Presence of a ketone.
.k

A small amount of the substance is taken in a test tube.

1 ml sodium nitro prusside solution is added. Then
w

sodium hydroxide solution is added dropwise.

Test for an amine.

w

14 Dye test: No Scarlet red dye is absence of an aromatic primary amine

obtained.
w

Take A small amount of an organic substance in a clean

test tube, add 2 ml of HCl to dissolve it. Add few
crystals of NaNO2, and cool the mixture in ice bath.
Then add 2 ml ofice cold solution of β-naphtholin
NaOH.

Test for diamide

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15 Biuret test: No Violet colour is appeared. absence of a diamide

Take A small amount of an organic compound
in a test tube. Heat strongly and then allow to
cool. Dissolve the residue with 2 ml of water. To
this solution Add 1 ml of dilute copper sulphate
solution and few drops of 10% NaOH solution
drop by drop.

Test for carbohydrates

16 Molisch’s test: No Violet or purple ring is formed at absence of carbohydrate

the junction of the two liquids.
Take A small amount of an organic compound
in a test tube. It is dissolved in 2 ml of water.
Add 3-4 drops of alpha naphtholto it.Then add
conc H2SO4 through the sides of test tube
carefully.

in
17 Osazone test: No Yellow crystals are obtained absence of carbohydrate

s.
Take A small amount of an organic compound
in a test tube. Add 1 ml of phenyl hydrazine
solution and heat the mixture for about 5

s
minutes on a boiling water bath.

re
xp
Result:
The given organic compound contains /is
e
(i) Aliphatic
vi

(ii) Saturated
al

(iii) ketone functional group (acetophenone )

.k
w
w
w

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Organic qualitative analysis - 4 (4.benzophenone)

S.no Experiment Observation Inference

Preliminary tests

1 Odour:
No characteristics odour Absence of amine,phenol,ester and
Note the Odour of the organic compound. benzaldehyde

2 Reaction with litmus paper:

No colour change is noted Absence of carboxylic acid , phenol
Touch the Moist litmus paper with an organic and amine
compound.

3 Action with sodium bicarbonate:

in
No brisk effervescence is Absence of a carboxylic acid.
Take 2 ml of saturated sodium bi carbonate obtained
solution in a test tube. Add 2 or 3 drops (or a

s.
pinch of solid) of an organic compound to it.

Action with Borsche’s reagent:

s
4
Take a small amount of an organic compound in yellow or orange or red Presence of an aldehyde or ketone

re
a test tube. Add 3 ml of Borsche’s reagent, 1 ml precipitate
of Conc HCl to it, then warm the mixture gently
and cool it.
xp
5 Charring test: Charring does not takes Absence of carbohydrate
place with smell of burnt
e
Take a small amount of an organic compound sugar
in a dry test tube. Add 2 ml of conc H2SO4 to
vi

it, and heat the mixture.

Tests for Aliphatic or Aromatic nature:

al

6 Ignition test:
.k

Burns with sooty flame Presence of an aromatic compound

Take small amount of the organic compound in
a Nickel spatula and burn it in Bunsen flame.
w

Tests for an unsaturation:

w

7 Test with bromine water:

w

Take small amount of the organic compound in No Decolourisation takes Substance is saturated.
a test tube add 2 ml of distilled water to place
dissolve it. To this solution add few drops of
bromine water and shake it well.

8 Test with KMnO4 solution:

Take small amount of the organic compound in No Decolourisation takes Substance is saturated.
a test tube add 2 ml of distilled water to place
dissolve it. To this solution add few drops of
very dilute alkaline KmnO4 solution and shake
it well.

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TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

Test For Phenol

9 Neutral FeCl3 test:

No Violet or blue absence of phenolic compound
Take 1 ml of neutral ferric chloride solution colouration is seen
is taken in a dry clean test tube. Add 2 or 3
drops (or a pinch of solid) oforganic
compound to it. If no colouration occurs add
3 or 4 drops of alcohol.

TEST FOR CARBOXYLIC ACIDS

10 Esterification reaction: No Pleasant fruity abesence of carboxylic group.

odour is noted.
Take 1 ml (or a pinch of solid) of an organic
compoundin a clean test tube. Add 1 ml of ethyl

in
alcohol and 4 to 5 drops of conc. sulphuric acid
to it. Heat the reaction mixture strongly for
about 5 minutes. Then pour the mixture into a

s.
beaker containing dil. Sodium carbonate
solution and note the smell.

s
Test for aldehydes.

re
11 Tollen’s reagent test: No Shining silver absence of an aldehyde
mirror is formed.
Take 2 ml of Tollen’s reagent in a clean dry test
tube. Add 3-4 drops of an organic compound (or
xp
0.2 g of solid) to it, and warm the mixture on a
water bath for about 5 minutes.
12 Fehling’s test: No Red precipitate is absence of an aldehyde
e
formed.
Take 1 ml each of Fehling’s solution A and B are
vi

taken in a test tube. Add 4-5 drops of an organic

compound (or 0.2g of solid) to it, and warm the
mixture on a water bath for about 5 minutes.
al

Test for ketones

Red colouration. Presence of a ketone.
.k

13 Legal’s test:
A small amount of the substance is taken in a test
w

tube. 1 ml sodium nitro prusside solution is

added. Then sodium hydroxide solution is added
dropwise.
w

Test for an amine.

14 Dye test: No Scarlet red dye is absence of an aromatic primary
w

obtained. amine
Take A small amount of an organic substance in a
clean test tube, add 2 ml of HCl to dissolve it.
Add few crystals of NaNO2, and cool the mixture
in ice bath. Then add 2 ml ofice cold solution of
β-naphtholin NaOH.

Test for diamide

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15 Biuret test: No Violet colour is appeared. absence of a diamide

Take A small amount of an organic compound in
a test tube. Heat strongly and then allow to cool.
Dissolve the residue with 2 ml of water. To this
solution Add 1 ml of dilute copper sulphate
solution and few drops of 10% NaOH solution
drop by drop.

Test for carbohydrates

16 Molisch’s test: No Violet or purple ring is absence of carbohydrate

formed at the junction of the
Take A small amount of an organic compound in two liquids.
a test tube. It is dissolved in 2 ml of water. Add 3-
4 drops of alpha naphtholto it.Then add conc
H2SO4 through the sides of test tube carefully.

in
17 Osazone test: No Yellow crystals are absence of carbohydrate
obtained
Take A small amount of an organic compound in

s.
a test tube. Add 1 ml of phenyl hydrazine solution
and heat the mixture for about 5 minutes on a
boiling water bath.

s
re
Result:
The given organic compound contains /is
xp
(i) Aromatic
e
(ii) Saturated
vi

(iii) ketone functional group (benzophenone )

al
.k
w
w
w

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Organic qualitative analysis - (5.benzoic acid)

S.no Experiment Observation Inference

Preliminary tests

1 Odour: Absence of
No characteristics odour benzaldehyde,amine,phenol and
Note the Odour of the organic compound. ester

2 Reaction with litmus paper:

Blue litmus turns red May be a carboxylic acid or
Touch the Moist litmus paper with an organic phenol
compound.

3 Action with sodium bicarbonate:

in
brisk effervescence is presence of a carboxylic acid.
Take 2 ml of saturated sodium bi carbonate solution obtained
in a test tube. Add 2 or 3 drops (or a pinch of solid)

s.
of an organic compound to it.

s
4 Action with Borsche’s reagent: No yellow or orange or red absence of an aldehyde or
precipitate ketone

re
Take a small amount of an organic compound in a
test tube. Add 3 ml of Borsche’s reagent, 1 ml of
Conc HCl to it, then warm the mixture gently and
cool it.
xp
5 Charring test: No Charring takes place with abesence of carbohydrate
smell of burnt sugar
e
Take a small amount of an organic compound in a
dry test tube. Add 2 ml of conc H2SO4 to it, and
vi

heat the mixture.

al

Tests for Aliphatic or Aromatic nature:

.k

6 Ignition test:
Burns with sooty flame Presence of an aromatic
Take small amount of the organic compound in a compound
Nickel spatula and burn it in Bunsen flame.
w

Tests for an unsaturation:

w

7 Test with bromine water:

No Decolourisation takes Substance is saturated.
w

Take small amount of the organic compound in a place

test tube add 2 ml of distilled water to dissolve it.
To this solution add few drops of bromine water
and shake it well.

8 Test with KMnO4 solution:

No Decolourisation takes Substance is saturated.
Take small amount of the organic compound in a place
test tube add 2 ml of distilled water to dissolve it.
To this solution add few drops of very dilute
alkaline KmnO4 solution and shake it well.

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TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

Test For Phenol

9 Neutral FeCl3 test:

No violet or blue colouration absence of phenol.
Take 1 ml of neutral ferric chloride solution is is seen
taken in a dry clean test tube. Add 2 or 3 drops (or a
pinch of solid) oforganic compound to it. If no
colouration occurs add 3 or 4 drops of alcohol.

TEST FOR CARBOXYLIC ACIDS

10 Esterification reaction:
Pleasant fruity odour is presence of carboxylic group.
Take 1 ml (or a pinch of solid) of an organic noted.
compoundin a clean test tube. Add 1 ml of ethyl
alcohol and 4 to 5 drops of conc. sulphuric acid to it.
Heat the reaction mixture strongly for about 5

in
minutes. Then pour the mixture into a beaker
containing dil. Sodium carbonate solution and note the
smell.

s.
Test for aldehydes.

s
11 Tollen’s reagent test:
No Shining silver mirror is absence of an aldehyde
Take 2 ml of Tollen’s reagent in a clean dry test tube.

re
formed.
Add 3-4 drops of an organic compound (or 0.2 g of
solid) to it, and warm the mixture on a water bath for
about 5 minutes.
xp
12 Fehling’s test:
No Red precipitate is formed. absence of an aldehyde
Take 1 ml each of Fehling’s solution A and B are
e
taken in a test tube. Add 4-5 drops of an organic
compound (or 0.2g of solid) to it, and warm the
vi

mixture on a water bath for about 5 minutes.

Test for ketones
al

13 Legal’s test:
Red colouration. presence of a ketone.
A small amount of the substance is taken in a test
.k

tube. 1 ml sodium nitro prusside solution is added.

Then sodium hydroxide solution is added dropwise.
w

Test for an amine.

Scarlet red dye is not absence of an aromatic
w

14 Dye test:
obtained. primary amine
Take A small amount of an organic substance in a clean
w

test tube, add 2 ml of HCl to dissolve it. Add few

crystals of NaNO2, and cool the mixture in ice bath.
Then add 2 ml ofice cold solution of β-naphtholin
NaOH.

Test for diamide

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15 Biuret test:
Take A small amount of an organic compound in a No Violet colour is appeared. absence of a diamide
test tube. Heat strongly and then allow to cool.
Dissolve the residue with 2 ml of water. To this
solution Add 1 ml of dilute copper sulphate
solution and few drops of 10% NaOH solution drop
by drop.

Test for carbohydrates

16 Molisch’s test:
Take A small amount of an organic compound in a No Violet or purple ring is absence of carbohydrate
test tube. It is dissolved in 2 ml of water. Add 3-4 formed at the junction of the
drops of alpha naphtholto it.Then add conc H2SO4 two liquids.
through the sides of test tube carefully.

in
17 Osazone test:
Take A small amount of an organic compound in a No Yellow crystals are absence of carbohydrate

s.
test tube. Add 1 ml of phenyl hydrazine solution and obtained
heat the mixture for about 5 minutes on a boiling
water bath.

s
re
Result:
The given organic compound contains /is
xp
(i) Aromatic
(ii) Saturated
e
(iii) carboxylic acid functional group ( benzoic acid)
vi
al
.k
w
w
w

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Organic qualitative analysis - 6 (6.cinnamic acid)

S.no Experiment Observation Inference

Preliminary tests

1 Odour: Absence of
No characteristics odour benzaldehyde,amine,phenol and
Note the Odour of the organic compound. ester

2 Reaction with litmus paper:

Blue litmus turns red May be a carboxylic acid or phenol
Touch the Moist litmus paper with an
organic compound.

in
3 Action with sodium bicarbonate:
brisk effervescence is presence of a carboxylic acid.
Take 2 ml of saturated sodium bi carbonate obtained

s.
solution in a test tube. Add 2 or 3 drops (or a
pinch of solid) of an organic compound to it.

s
4 Action with Borsche’s reagent: No yellow or orange or red absence of an aldehyde or ketone
precipitate

re
Take a small amount of an organic
compound in a test tube. Add 3 ml of
Borsche’s reagent, 1 ml of Conc HCl to it,
then warm the mixture gently and cool it.
e xp
Charring takes place with presence of carbohydrate
5 Charring test:
vi

smell of burnt sugar

Take a small amount of an organic
compound in a dry test tube. Add 2 ml of
al

conc H2SO4 to it, and heat the mixture.

Tests for Aliphatic or Aromatic nature:

.k

6 Ignition test:
w

Burns with sooty flame Presence of an aromatic compound

Take small amount of the organic
compound in a Nickel spatula and burn it in
w

Bunsen flame.

Tests for an unsaturation:

w

7 Test with bromine water:

Decolourisation takes place Substance is unsaturated.
Take small amount of the organic
compound in a test tube add 2 ml of
distilled water to dissolve it. To this solution
add few drops of bromine water and shake it
well.

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8 Test with KMnO4 solution:

Decolourisation takes place Substance is unsaturated.
Take small amount of the organic
compound in a test tube add 2 ml of
distilled water to dissolve it. To this solution
add few drops of very dilute alkaline
KmnO4 solution and shake it well.

TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

Test For Phenol

9 Neutral FeCl3 test:

No violet or blue colouration absence of phenol.
Take 1 ml of neutral ferric chloride solution is seen
is taken in a dry clean test tube. Add 2 or 3
drops (or a pinch of solid) oforganic
compound to it. If no colouration occurs add

in
3 or 4 drops of alcohol.

TEST FOR CARBOXYLIC ACIDS

s.
10 Esterification reaction:
Pleasant fruity odour is noted. presence of carboxylic group.
Take 1 ml (or a pinch of solid) of an organic

s
compoundin a clean test tube. Add 1 ml of

re
ethyl alcohol and 4 to 5 drops of conc.
sulphuric acid to it. Heat the reaction
mixture strongly for about 5 minutes. Then
pour the mixture into a beaker containing
xp
dil. Sodium carbonate solution and note the
smell.
Test for aldehydes.
e
11 Tollen’s reagent test:
vi

No Shining silver mirror is absence of an aldehyde

Take 2 ml of Tollen’s reagent in a clean dry formed.
test tube. Add 3-4 drops of an organic
al

compound (or 0.2 g of solid) to it, and warm

the mixture on a water bath for about 5
minutes.
.k

12 Fehling’s test:
No Red precipitate is formed. absence of an aldehyde
Take 1 ml each of Fehling’s solution A and
w

B are taken in a test tube. Add 4-5 drops of

an organic compound (or 0.2g of solid) to it,
w

and warm the mixture on a water bath for

about 5 minutes.
w

Test for ketones

13 Legal’s test:
No Red colouration. absence of a ketone.
A small amount of the substance is taken in a
test tube. 1 ml sodium nitro prusside solution
is added. Then sodium hydroxide solution is
added dropwise.
Test for an amine.

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14 Dye test: Scarlet red dye is not obtained. absence of an aromatic primary
amine
Take A small amount of an organic substance
in a clean test tube, add 2 ml of HCl to
dissolve it. Add few crystals of NaNO2, and
cool the mixture in ice bath. Then add 2 ml
ofice cold solution of β-naphtholin NaOH.

Test for diamide

15 Biuret test:
Take A small amount of an organic No Violet colour is appeared. absence of a diamide
compound in a test tube. Heat strongly and
then allow to cool. Dissolve the residue with
2 ml of water. To this solution Add 1 ml of
dilute copper sulphate solution and few drops
of 10% NaOH solution drop by drop.

in
Test for carbohydrates

s.
16 Molisch’s test:
Take A small amount of an organic compound No Violet or purple ring is absence of carbohydrate

s
in a test tube. It is dissolved in 2 ml of water. formed at the junction of the

re
Add 3-4 drops of alpha naphtholto it.Then add two liquids.
conc H2SO4 through the sides of test tube
carefully.
xp
17 Osazone test:
Take A small amount of an organic compound No Yellow crystals are absence of carbohydrate
e
in a test tube. Add 1 ml of phenyl hydrazine obtained
solution and heat the mixture for about 5
vi

minutes on a boiling water bath.

al

Result:
The given organic compound contains /is
.k

(i) Aromatic
w

(ii) Saturated
(iii) carboxylic acid functional group ( cinnamic acid)
w
w

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Organic qualitative analysis - 7 (7.urea)
S.no Experiment Observation Inference
Preliminary tests

1 Odour:
No characteristics odour Absence of amine,phenol,benzaldehyde
Note the Odour of the organic compound. and ester

2 Reaction with litmus paper:

No colour change is noted
Touch the Moist litmus paper with an organic Absence of carboxylic acid , phenol and
compound. amine

3 Action with sodium bicarbonate:

No brisk effervescence is Absence of a carboxylic acid.
Take 2 ml of saturated sodium bi carbonate obtained
solution in a test tube. Add 2 or 3 drops (or a

in
pinch of solid) of an organic compound to it.

s.
4 Action with Borsche’s reagent:
Take a small amount of an organic compound No yellow or orange or red absence of an aldehyde or ketone

s
in a test tube. Add 3 ml of Borsche’s reagent, 1 precipitate
ml of Conc HCl to it, then warm the mixture

re
gently and cool it.

5 Charring test:
xp
Take a small amount of an organic compound No Charring takes place with absence of carbohydrate
in a dry test tube. Add 2 ml of conc H2SO4 to smell of burnt sugar
e
it, and heat the mixture.
vi

Tests for Aliphatic or Aromatic nature:

al

6 Ignition test:
Burns with not-sooty flame Presence of an aliphatic compound
Take small amount of the organic compound in
a Nickel spatula and burn it in Bunsen flame.
.k

Tests for an unsaturation:

w

7 Test with bromine water:

No Decolourisation takes Substance is saturated.
w

Take small amount of the organic compound in place

a test tube add 2 ml of distilled water to .
w

dissolve it. To this solution add few drops of

bromine water and shake it well.

8 Test with KMnO4 solution:

No Decolourisation takes Substance is saturated.
Take small amount of the organic compound in place
a test tube add 2 ml of distilled water to
dissolve it. To this solution add few drops of
very dilute alkaline KmnO4 solution and shake
it well.

TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

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Test For Phenol

9 Neutral FeCl3 test:

No Violet or blue colouration absence of phenolic compound
Take 1 ml of neutral ferric chloride solution is is seen
taken in a dry clean test tube. Add 2 or 3 drops
(or a pinch of solid) oforganic compound to it.
If no colouration occurs add 3 or 4 drops of
alcohol.

TEST FOR CARBOXYLIC ACIDS

10 Esterification reaction: No Pleasant fruity odour is absence of carboxylic group.
noted.
Take 1 ml (or a pinch of solid) of an organic
compoundin a clean test tube. Add 1 ml of ethyl
alcohol and 4 to 5 drops of conc. sulphuric acid
to it. Heat the reaction mixture strongly for
about 5 minutes. Then pour the mixture into a

in
beaker containing dil. Sodium carbonate solution
and note the smell.
Test for aldehydes.

s.
11 Tollen’s reagent test: No Shining silver mirror is absence of an aldehyde
formed.
Take 2 ml of Tollen’s reagent in a clean dry test

s
tube. Add 3-4 drops of an organic compound (or

re
0.2 g of solid) to it, and warm the mixture on a
water bath for about 5 minutes.
12 Fehling’s test:
xp
No Red precipitate is formed. absence of an aldehyde
Take 1 ml each of Fehling’s solution A and B
are taken in a test tube. Add 4-5 drops of an
organic compound (or 0.2g of solid) to it, and
e
warm the mixture on a water bath for about 5
vi

minutes.
Test for ketones
al

13 Legal’s test:
No Red colouration. absence of a ketone.
A small amount of the substance is taken in a
.k

test tube. 1 ml sodium nitro prusside solution is

added. Then sodium hydroxide solution is added
dropwise.
w

Test for an amine.

w

14 Dye test:
Take A small amount of an organic substance in No Scarlet red dye is absence of an aromatic primary amine
w

a clean test tube, add 2 ml of HCl to dissolve it. obtained.

Add few crystals of NaNO2, and cool the
mixture in ice bath. Then add 2 ml ofice cold
solution of β-naphtholin NaOH.

Test for diamide

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15 Biuret test:
Take A small amount of an organic compound Violet colour is appeared. presence of a diamide
in a test tube. Heat strongly and then allow to
cool. Dissolve the residue with 2 ml of water. To
this solution Add 1 ml of dilute copper sulphate
solution and few drops of 10% NaOH solution
drop by drop.

Test for carbohydrates

16 Molisch’s test:
Take A small amount of an organic compound No Violet or purple ring is absence of carbohydrate
in a test tube. It is dissolved in 2 ml of water. formed at the junction of the
Add 3-4 drops of alpha naphtholto it.Then add two liquids.
conc H2SO4 through the sides of test tube
carefully.

in
17 Osazone test:

s.
Take A small amount of an organic compound No Yellow crystals are absence of carbohydrate
in a test tube. Add 1 ml of phenyl hydrazine obtained
solution and heat the mixture for about 5

s
minutes on a boiling water bath.

re
Result:
xp
The given organic compound contains /is

(i) aliphatic
e
(ii) Saturated
vi

(iii) diamide functional group (urea )

al
.k
w
w
w

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Organic qualitative analysis - 8 (8.clucose)

S.no Experiment Observation Inference

Preliminary tests

1 Odour:
No characteristics odour Absence of
Note the Odour of the organic compound. amine,phenol,benzaldehy
de and ester

2 Reaction with litmus paper:

No colour change is noted
Touch the Moist litmus paper with an organic compound. Absence of carboxylic
acid , phenol and amine

3 Action with sodium bicarbonate:

in
No brisk effervescence is Absence of a carboxylic
Take 2 ml of saturated sodium bi carbonate solution in a obtained acid.
test tube. Add 2 or 3 drops (or a pinch of solid) of an

s.
organic compound to it.

Action with Borsche’s reagent:

s
4
Take a small amount of an organic compound in a test

re
yellow or orange or red presence of an aldehyde
tube. Add 3 ml of Borsche’s reagent, 1 ml of Conc HCl to precipitate or ketone
it, then warm the mixture gently and cool it. xp
5 Charring test:
Take a small amount of an organic compound in a dry test Charring takes place with smell presence of carbohydrate
e
tube. Add 2 ml of conc H2SO4 to it, and heat the mixture. of burnt sugar
vi

Tests for Aliphatic or Aromatic nature:

al

6 Ignition test:
Burns with not-sooty flame Presence of an aliphatic
Take small amount of the organic compound in a Nickel compound
.k

spatula and burn it in Bunsen flame.

Tests for an unsaturation:

w

7 Test with bromine water:

Decolourisation takes place Substance is unsaturated.
w

Take small amount of the organic compound in a test tube .

add 2 ml of distilled water to dissolve it. To this solution
w

add few drops of bromine water and shake it well.

8 Test with KMnO4 solution:

Decolourisation takes place Substance is unsaturated.
Take small amount of the organic compound in a test tube
add 2 ml of distilled water to dissolve it. To this solution
add few drops of very dilute alkaline KmnO4 solution and
shake it well.

TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

Test For Phenol

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9 Neutral FeCl3 test:

No Violet or blue colouration is absence of phenolic
Take 1 ml of neutral ferric chloride solution is taken in a seen compound
dry clean test tube. Add 2 or 3 drops (or a pinch of solid)
oforganic compound to it. If no colouration occurs add 3
or 4 drops of alcohol.

TEST FOR CARBOXYLIC ACIDS

10 Esterification reaction:
No Pleasant fruity odour is absence of carboxylic
Take 1 ml (or a pinch of solid) of an organic compoundin noted. group.
a clean test tube. Add 1 ml of ethyl alcohol and 4 to 5
drops of conc. sulphuric acid to it. Heat the reaction
mixture strongly for about 5 minutes. Then pour the
mixture into a beaker containing dil. Sodium carbonate
solution and note the smell.

Test for aldehydes.

in
11 Tollen’s reagent test: Shining silver mirror is formed. presence of an aldehyde
Take 2 ml of Tollen’s reagent in a clean dry test tube. Add

s.
3-4 drops of an organic compound (or 0.2 g of solid) to it,
and warm the mixture on a water bath for about 5 minutes.

s
12 Fehling’s test: Red precipitate is formed. absence of an aldehyde

re
Take 1 ml each of Fehling’s solution A and B are taken in
a test tube. Add 4-5 drops of an organic compound (or
0.2g of solid) to it, and warm the mixture on a water bath
xp
for about 5 minutes.
Test for ketones
No Red colouration. absence of a ketone.
Legal’s test:
e
13
A small amount of the substance is taken in a test tube. 1
vi

ml sodium nitro prusside solution is added. Then sodium

hydroxide solution is added dropwise.
al

Test for an amine.

14 Dye test: No Scarlet red dye is obtained. absence of an aromatic
.k

primary amine
Take A small amount of an organic substance in a clean
test tube, add 2 ml of HCl to dissolve it. Add few crystals
w

of NaNO2, and cool the mixture in ice bath. Then add 2 ml

ofice cold solution of β-naphtholin NaOH.
w

Test for diamide

w

15 Biuret test: No Violet colour is appeared. absence of a diamide

Take A small amount of an organic compound in a test
tube. Heat strongly and then allow to cool. Dissolve the
residue with 2 ml of water. To this solution Add 1 ml of
dilute copper sulphate solution and few drops of 10%
NaOH solution drop by drop.

Test for carbohydrates

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16 Molisch’s test: Violet or purple ring is formed at presence of carbohydrate

the junction of the two liquids.
Take A small amount of an organic compound in a test
tube. It is dissolved in 2 ml of water. Add 3-4 drops of
alpha naphtholto it.Then add conc H2SO4 through the
sides of test tube carefully.

17 Osazone test: Yellow crystals are obtained presence of carbohydrate

Take A small amount of an organic compound in a test
tube. Add 1 ml of phenyl hydrazine solution and heat the
mixture for about 5 minutes on a boiling water bath.

Result:
The given organic compound contains /is

in
(i) aliphatic
(ii) Saturated

s.
(iii) aldehyde functional group (glucose )

s
re
e xp
vi
al
.k
w
w
w

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Organic qualitative analysis – 9 (9.aniline)

S.n Experiment Observation Inference

o
Preliminary tests

1 Odour:
Fish odour May be amine
Note the Odour of the organic compound.

2 Reaction with litmus paper:

Red litmus turns blue May be amine
Touch the Moist litmus paper with an organic
compound.

in
3 Action with sodium bicarbonate:
No brisk effervescence is obtained Absence of a carboxylic
Take 2 ml of saturated sodium bi carbonate solution in acid.
a test tube. Add 2 or 3 drops (or a pinch of solid) of an

s.
organic compound to it.

s
4 Action with Borsche’s reagent:

re
Take a small amount of an organic compound in a test No yellow or orange or red absence of an aldehyde or
tube. Add 3 ml of Borsche’s reagent, 1 ml of Conc precipitate ketone
HCl to it, then warm the mixture gently and cool it.
xp
5 Charring test:
Take a small amount of an organic compound in a dry No Charring takes place with absence of carbohydrate
e
test tube. Add 2 ml of conc H2SO4 to it, and heat the smell of burnt sugar
mixture.
vi

Tests for Aliphatic or Aromatic nature:

al

6 Ignition test:
Burns with sooty flame Presence of an aromatic
.k

Take small amount of the organic compound in a Nickel compound

spatula and burn it in Bunsen flame.
w

Tests for an unsaturation:

w

7 Test with bromine water:

No Decolourisation takes place Substance is saturated.
Take small amount of the organic compound in a test .
w

tube add 2 ml of distilled water to dissolve it. To this

solution add few drops of bromine water and shake it
well.

8 Test with KMnO4 solution:

No Decolourisation takes place Substance is saturated.
Take small amount of the organic compound in a test
tube add 2 ml of distilled water to dissolve it. To this
solution add few drops of very dilute alkaline KmnO4
solution and shake it well.

TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

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Test For Phenol

9 Neutral FeCl3 test:

No Violet or blue colouration is absence of phenolic
Take 1 ml of neutral ferric chloride solution is taken in a seen compound
dry clean test tube. Add 2 or 3 drops (or a pinch of solid)
oforganic compound to it. If no colouration occurs add 3
or 4 drops of alcohol.

TEST FOR CARBOXYLIC ACIDS

10 Esterification reaction: No Pleasant fruity odour is absence of carboxylic
noted. group.
Take 1 ml (or a pinch of solid) of an organic
compoundin a clean test tube. Add 1 ml of ethyl alcohol
and 4 to 5 drops of conc. sulphuric acid to it. Heat the
reaction mixture strongly for about 5 minutes. Then pour
the mixture into a beaker containing dil. Sodium
carbonate solution and note the smell.

in
Test for aldehydes.
No Shining silver mirror is absence of an aldehyde

s.
11 Tollen’s reagent test:
formed.
Take 2 ml of Tollen’s reagent in a clean dry test tube.

s
Add 3-4 drops of an organic compound (or 0.2 g of
solid) to it, and warm the mixture on a water bath for

re
about 5 minutes.
12 Fehling’s test: No Red precipitate is formed. absence of an aldehyde

Take 1 ml each of Fehling’s solution A and B are taken

xp
in a test tube. Add 4-5 drops of an organic compound (or
0.2g of solid) to it, and warm the mixture on a water bath
for about 5 minutes.
e
Test for ketones
vi

13 Legal’s test: No Red colouration. absence of a ketone.

A small amount of the substance is taken in a test tube. 1

al

ml sodium nitro prusside solution is added. Then sodium

hydroxide solution is added dropwise.
.k

Test for an amine.

14 Dye test: Scarlet red dye is obtained. presence of an aromatic
primary amine
w

Take A small amount of an organic substance in a clean

test tube, add 2 ml of HCl to dissolve it. Add few
w

crystals of NaNO2, and cool the mixture in ice bath.

Then add 2 ml ofice cold solution of β-naphtholin
NaOH.
w

Test for diamide

15 Biuret test:
Take A small amount of an organic compound in a test No Violet colour is appeared. absence of a diamide
tube. Heat strongly and then allow to cool. Dissolve the
residue with 2 ml of water. To this solution Add 1 ml of
dilute copper sulphate solution and few drops of 10%
NaOH solution drop by drop.

Test for carbohydrates

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16 Molisch’s test:
Take A small amount of an organic compound in a test No Violet or purple ring is absence of carbohydrate
tube. It is dissolved in 2 ml of water. Add 3-4 drops of formed at the junction of the
alpha naphtholto it.Then add conc H2SO4 through the two liquids.
sides of test tube carefully.

17 Osazone test:
Take A small amount of an organic compound in a test No Yellow crystals are absence of carbohydrate
tube. Add 1 ml of phenyl hydrazine solution and heat obtained
the mixture for about 5 minutes on a boiling water bath.

Result:
The given organic compound contains /is

in
(i) aromatic
(ii) Saturated

s.
(iii) amine functional group (aniline )

s
re
e xp
vi
al
.k
w
w
w

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Organic qualitative analysis - 10 (10.salicylic acid)

S.no Experiment Observation Inference

Preliminary tests

1 Odour: Absence of
No characteristics odour benzaldehyde,amine,phenol
Note the Odour of the organic compound. and ester

2 Reaction with litmus paper:

Blue litmus turns red May be a carboxylic acid or
Touch the Moist litmus paper with an organic phenol
compound.

3 Action with sodium bicarbonate:

brisk effervescence is obtained presence of a carboxylic
Take 2 ml of saturated sodium bi carbonate

in
acid.
solution in a test tube. Add 2 or 3 drops (or a
pinch of solid) of an organic compound to it.

s.
4 Action with Borsche’s reagent: No yellow or orange or red absence of an aldehyde or
precipitate ketone

s
Take a small amount of an organic compound in a
test tube. Add 3 ml of Borsche’s reagent, 1 ml of

re
Conc HCl to it, then warm the mixture gently and
cool it. xp
5 Charring test: No Charring takes place with smell absence of carbohydrate
of burnt sugar
Take a small amount of an organic compound in
e
a dry test tube. Add 2 ml of conc H2SO4 to it,
and heat the mixture.
vi

Tests for Aliphatic or Aromatic nature:

al

6 Ignition test:
Burns with sooty flame Presence of an aromatic
Take small amount of the organic compound in a
.k

compound
Nickel spatula and burn it in Bunsen flame.
w

Tests for an unsaturation:

w

7 Test with bromine water:

Decolourisation with formation of Presence of an aromatic
Take small amount of the organic compound in a white precipitate amine or phenol.
w

test tube add 2 ml of distilled water to dissolve it.

To this solution add few drops of bromine water
and shake it well.

8 Test with KMnO4 solution:

No Decolourisation takes place Substance is saturated.
Take small amount of the organic compound in a
test tube add 2 ml of distilled water to dissolve it.
To this solution add few drops of very dilute
alkaline KmnO4 solution and shake it well.

TEST FOR SELECTED ORGANIC FUNCTIONAL GROUPS

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Test For Phenol

9 Neutral FeCl3 test:

violet colouration is seen presence of phenol.
Take 1 ml of neutral ferric chloride solution is
taken in a dry clean test tube. Add 2 or 3 drops
(or a pinch of solid) oforganic compound to it. If
no colouration occurs add 3 or 4 drops of alcohol.

TEST FOR CARBOXYLIC ACIDS

10 Esterification reaction:
Pleasant fruity odour is noted. presence of carboxylic
Take 1 ml (or a pinch of solid) of an organic group.
compoundin a clean test tube. Add 1 ml of ethyl
alcohol and 4 to 5 drops of conc. sulphuric acid to
it. Heat the reaction mixture strongly for about 5
minutes. Then pour the mixture into a beaker
containing dil. Sodium carbonate solution and

in
note the smell.
Test for aldehydes.

s.
11 Tollen’s reagent test:
No Shining silver mirror is formed. absence of an aldehyde
Take 2 ml of Tollen’s reagent in a clean dry test

s
tube. Add 3-4 drops of an organic compound (or
0.2 g of solid) to it, and warm the mixture on a

re
water bath for about 5 minutes.
12 Fehling’s test:
No Red precipitate is formed. absence of an aldehyde
Take 1 ml each of Fehling’s solution A and B are
xp
taken in a test tube. Add 4-5 drops of an organic
compound (or 0.2g of solid) to it, and warm the
e
mixture on a water bath for about 5 minutes.
Test for ketones
vi

13 Legal’s test:
No Red colouration. absence of a ketone.
A small amount of the substance is taken in a
al

test tube. 1 ml sodium nitro prusside solution is

added. Then sodium hydroxide solution is added
.k

dropwise.
Test for an amine.
w

14 Dye test:
Take A small amount of an organic substance in Scarlet red dye is not obtained. absence of an aromatic
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a clean test tube, add 2 ml of HCl to dissolve it. primary amine

Add few crystals of NaNO2, and cool the
mixture in ice bath. Then add 2 ml ofice cold
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solution of β-naphtholin NaOH.

Test for diamide

15 Biuret test:
Take A small amount of an organic compound in a No Violet colour is appeared. absence of a diamide
test tube. Heat strongly and then allow to cool.
Dissolve the residue with 2 ml of water. To this
solution Add 1 ml of dilute copper sulphate solution
and few drops of 10% NaOH solution drop by drop.

Test for carbohydrates

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16 Molisch’s test:
Take A small amount of an organic compound in a No Violet or purple ring is formed absence of carbohydrate
test tube. It is dissolved in 2 ml of water. Add 3-4 at the junction of the two liquids.
drops of alpha naphtholto it.Then add conc H2SO4
through the sides of test tube carefully.

17 Osazone test:
Take A small amount of an organic compound in a No Yellow crystals are obtained absence of carbohydrate
test tube. Add 1 ml of phenyl hydrazine solution and
heat the mixture for about 5 minutes on a boiling
water bath.

Result:
The given organic compound contains is

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(i) Aromatic
(ii) Saturated

s.
(iii) phenol,carboxylic acid functional group (salicylic acid)

s
re
e xp
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al
.k
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w
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s s.
re
e xp
vi
al
.k
w
w
w

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in
s s.
re
e xp
vi
al
.k
w
w
w

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in
s s.
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e xp
vi
al
.k
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w
w

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