COURSE STRUCTURE
MSc, Microbiology
SEM COMPONENT SUB CODE TITLE OF THE PAPER HRS/ CREDIT
WEEK
Core І 09PMBC11 General microbiology 6 4
Core І І 09PMBC12 Microbial Diversity And Classification 6 4
І Core III 09PMBC13 Bacteriology 6 4
Elective І 09PMBE11 Bioinstrumentation and Techniques 6 5
Practical І General Microbiology, Microbial Diversity 6 -
and Bioinstrumentation
Total 30 17
Core І 09PMBC21 Microbial Physiology and Biochemistry 4 4
Core І I 09PMBC22 Immunology 4 4
Core ІII 09PMBC23 Medical Microbiology 4 4
Core І V 09PMBC24 Microbial Genetics and Molecular Biology 4 4
Elective І 09PMBE21 Computers and Biology 4 4
ІI Practical Exam І 09PMBCR1 Practical- І - 4
Practical Exam 09PMBCR2 Practical- ІI 5 4
ІІ Techniques in Microbial Physiology
Biochemistry and Molecular Biology
Practical Exam
09PMBCR3 Practical- ІII 5 4
ІІІ
Techniques in Immunology and Medical
Microbiology
Total 30 32
GENERAL MICROBIOLOGY
UNIT : I
Evolution of microbiology- contributions of Van Leeuwenhoek, Joseph Lister, Louis Pasteur
, Robert Koch, Edward Jenner, Winogradsky and Beijernick- Further developments in
microbiology- identification, characterization and classification of microorganisms- Distinguishing
characteristics between prokaryotic and eukaryotic cells- Phenotypic characters- Taxonomic
characters- Distinctive characters of major groups of microorganisms- Principles of classification-
Numerical taxonomy and taxonomic structuring- Genetic relationship- DNA homology- 16S rRNA
Sequencing.
UNIT : II
Microscopy- It’s principles and applications in the field of microbiology including the
following, Dark field, phase contrast, fluorescence microscopy, transmission and scanning electron
microscopy, confocal microscopy- colorimeter, spectrophotometer and lyophilizers- Staining
methods- Gram’s acid- fast, met-achromatic granules, nuclear, capsule, flagella, silver
impregnation and other special staining methods.
UNIT : III
Methods of sterilization: Physical and chemical agents, radiation and filtration- Indicator
microorganisms for sterilization methods- Cultivation of microorganisms- Microbiological media,
enrichment media, enriched media, transport media, selective media and pure culture technique-
Methods of preservation and maintenance of cultures- Role of disinfectants.
UNIT : IV
Bacterial anatomy, structure, properties and bio-synthesis of cellular components of
bacteria- Sporulation and it’s mechanism- Growth and nutrition- Nutritional requirements-
Autotrophs- Heterotrophs- Enrichment cultures- Growth curve- Kinetics of growth- Batch culture-
Synchronous growth- Measurement of growth and enumeration of cells- Techniques of pure
culture.
UNIT : V
Laboratory uses of animals with special reference to microbiology pathogenicity testing,
antibody production, and toxin/ toxoid testing, hypersensitivity testing, and maintenance of
microbes in animals- Care breeding and maintenance of laboratory animals: Rabbits, rats, mice,
guinea pigs, monkeys etc, Disposal of animal house wastes.
MICROBIAL DIVERSITY AND CLASSIFICATION
UNIT : I
Classification of microorganisms- Introduction- Haeckel’s three kingdom concept-
Whittaker’ five kingdom concept- Three domain concept of Carl Woese basis of microbial
classification, Salient features of bacteria according to Bergey’s manual of determinative
bacteriology- Cyanobacteria- Archaebacteria evolution of archaebacterial membranes, cell wall and
metabolism.
UNIT : II
Fungi: Morphology, taxonomy and classification- detection and recovery fungi from clinical
specimens- Dermatophytes and agents of superficial mycoses- Yeast of medical importance –
Mycotoxins- Anti-fungal agents- Testing methods and quality control. Dimorphic fungi causing
systematic Mycoses- Dermataceous fungi, opportunistic hyaline hypomycetes, agents of
Zygomycoses- Fungi causing eumycotic mycetoma- Immunity to fungal infections.
UNIT : III
Mycology: Historical introduction, structure and cell differentiation- Lichens: Ascolichens,
basidolichens and deuterolichens. Mycorrhiza- Ectomycorrhizae, endomycorrhizae, vesicular
arbuscular mycorrhizae- Fungi as symbionts.
UNIT : IV
Algae: Distribution, general characters, thallus and its structure, classification, nutrition and
reproduction- Characters of selected groups- Blue green algae, Dinofalgellates, Euglenophyta,
Chrysophyta, Phaeophyta and Rhodophyta- Economic importance of alga biotechnology.
UNIT : V
Protozoa Distinguishing characters, classification, host-parasite relationship, pathogenic
mechanism, transmission, life cycle, lab diagnosis, treatment for the following: Entamoeba sp
(Aerobic and anaerobic), Leishmania sp, Trypanasoma sp, Giardia sp, Trichomonas sp and
Balantidium sp. Helminthes: Classification, lifecycle, pathogenesis, transmission, lab diagnosis,
treatment for Cestodes (Taenia solium)- Trematodes ( Fasciola hepatica), Nematodes (Ascaris
lumbricoides, Wuchereia bancrofti)- Arthropod vectors: Tick, mites, fleas, mosquitoes and flies-
Biology, life cycle and transmission diseases to human.
BACTERIOLOGY
UNIT : I
Classification and identification of bacteria- Bacterial Nomenclature- Bergey’s of
Classification.
UNIT : II
Bacterial Photosynthesis- classification of photosynthetic bacteria- purple bacteria- purple
Sulphur bacteria- purple non sulphur bacteria- Green bacteria- Photosynthetic pigments-
Bacteriochlorophyll metabolism in photosynthetic bacteria.
UNIT : III
Bacteria with unusual properties filamentous bacteria of complex morphology.
UNIT : IV
Bacterial Pathogens- Bacterial toxins- Examples of toxins- caused pathogenesis- bacterial
colonization and invasion.
UNIT : V
Epidemiology of infections diseases- disease reservoirs of infection- modes of transmission-
diseases caused by endospore forming bacteria- enteric bacteria- Mycobacterial disease-
Legionaries disease- listeriosis- brucellosis- Tularemia.
BIOINSTRUMENTATION AND LABORATORY TECHNIQUES
UNIT : I
pH meter- Titration curve an measurement- Principles, laws of absorption and radiation,
visible, ultraviolet, infrared and mass spectrophotometry- Absorption spectrum, flame photometry-
Principles of colorimetry, turbidometry and viscometry.
UNIT : II
Principles of centrifugation- Centrifugation techniques- Preparative and analytical methods,
density gradient centrifugation- Types of rotors.
UNIT : III
General principle and applications of chromatography- Paper, thin layer, column, gel
filtration, affinity and ion exchange chromatography- Advanced techniques (GC/ HPLC).
UNIT : IV
Electrophoresis- Horizontal and vertical- Moving boundary, zone (paper and gel)
electrophoresis- Immunoelectrophoresis- Rocket electrophoresis- Isoelectric focusing- Application
of each.
UNIT : V
Advanced instrumentation- IR spectroscopy, Raman spectroscopy, x-ray spectroscopy,
NMR, AAS- Principles, component structure and applications. Radio isotopic tracers:
Methodology, problem of experimental design, radiometric analysis, stable and radioactive
isotopes, preparation, labeling, detection and measurement- Autoradiography.
