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Auto Radiography

Autoradiography is a technique used to visualize the distribution of radioactive substances in a biological sample. It works by incorporating radioactive materials into a specimen, which is then placed in contact with photographic emulsion. The radiation from the specimen exposes the emulsion, resulting in a radiograph that shows the exact location of the radioactive target within the tissue. Autoradiography can be used to locate specific materials, tissues, drugs, or metabolic activities within cells.

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229 views1 page

Auto Radiography

Autoradiography is a technique used to visualize the distribution of radioactive substances in a biological sample. It works by incorporating radioactive materials into a specimen, which is then placed in contact with photographic emulsion. The radiation from the specimen exposes the emulsion, resulting in a radiograph that shows the exact location of the radioactive target within the tissue. Autoradiography can be used to locate specific materials, tissues, drugs, or metabolic activities within cells.

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parthapratim
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© © All Rights Reserved
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Parthapratim

AUTORADIOGRAPHY

Autoradiography is a histo-chemical or bio-analytical technique used to visualize distribution of


radioactive labelled substance with radioisotope in a biological sample. It is a method by which a
radioactive material can be localized within a particular tissue, cell, cell organelle or biomolecules. It
is a very sensitive technique and is used in a wide variety of biological experiments. Autoradiography
can also be used for quantitative estimation.

Principle

Autoradiography is based on the ability of radioactive substance to expose a photographic film by


ionizing it. When silver halide is exposed to radioactive materials, the silver ions (Ag+) are reduced to
its metallic form (Ag) which gives it a dark colour. In this technique, a biological specimen acts as the
source of radiation. This is achieved by incorporating radioactive materials into the specimen. This
radioactive specimen is put in direct contact with a thick layer of photographic emulsion, made of
gelatine substances. This emulsion contains a high amount of crystals of silver halide. This emulsion
is left in dark for several days for proper exposure. When the silver halide crystals are exposed to the
radiation (from the sample), they are converted into metallic silver which gives it a dark colour. Now,
the emulsion with such colour bands is called a radiograph. The radiograph is viewed by means of
electron microscope, digital scanners, electrophoresis etc. This gives the exact location of the target
substance in the specimen.

Applications

i. To find the location and amount of a particular material inside a cell, such as cell organelles,
biomolecules, metabolites etc.
ii. To find location of particular tissue.
iii. To investigate location and performance of a particular drug.
iv. To find locations of particular metabolic activity inside a cell, such as replication, mitosis etc.
v. To find and investigate various properties of nucleic acids (DNA and RNA).

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