[go: up one dir, main page]
Scribd
Upload
151 views2 pages

Quickextract™ Dna Extraction Solution

QuickExtract

Uploaded by

san
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
151 views2 pages

Quickextract™ Dna Extraction Solution

QuickExtract

Uploaded by

san
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 2

PURIFICATION & EXTRACTION

lucigen.com/pur-ext

QuickExtract™ DNA Extraction Solution


Simple, rapid extraction of PCR-ready DNA

• Rapid Procedure: Eight-minute protocol for most sample types


• Simple Method: Single-tube protocol with no spin columns Exclusively available
thru Lucigen.
• Automation-Friendly: Process one or hundreds of samples
• Safe Workflow: No phenol, chloroform, or guanidinium salts
• Many Applications: Suitable for genotyping, human identity testing, viral/microbial screening, and more

The QuickExtract DNA Extraction Solution extracts PCR-ready genomic DNA from almost any sample in just 3–8 minutes.

Many publications support the use of QuickExtract DNA Extraction Solution with samples such as hair follicles, quill-end cells of feathers, tissue-culture
cells, buccal cells, zebrafish organs and scales, mouse tail snips, and more. The simple, single-tube procedure can accommodate one to hundreds of
samples, and it is easily adapted to multiwell plates with robotic automation systems.

The extracted DNA is suitable for PCR-based analysis, such as: genomic, transgenic, or viral DNA screening in animals; genetic or environmental research
and screening in humans and other organisms; and CRISPR/Cas9 library screening.

The convenient, scalable protocol involves gentle lysis and purification that provides high yields of intact nucleic acids—all without the use of toxic
chemicals or spin columns.

PCR-Ready DNA from a Variety of Samples

Figure 1. FailSafe™ PCR amplifications of genomic DNA isolated using


the QuickExtract™ procedure. All samples were treated with QuickExtract™
DNA Extraction Solution. PCR was performed using primers to amplify the
regions indicated: Lanes 1–3, human β-globin (from human buccal cells, HeLa
cells, and human hair follicle, respectively); lane 4, transgenic mouse GAPDH
(from mouse tail snip); lane 5, 16S ribosomal RNA gene (from E. coli); lane 6,
transgenic SV40 T antigen (from mouse tail snip).

For research or investigational use only. E103-NP0917


FM 598408
PURIFICATION & EXTRACTION
lucigen.com/pur-ext

Sensitive PCR Detection from Extracted DNA PCR-Ready DNA in 8 Minutes or Less

Add samples

QuickExtract™
Solution

Heat at 65˚C for 6 minutes


and 98˚C for 2 minutes

Figure 2. PCR amplification of DNA extracted from multiple zebrafish (Danio rerio)
organs using QuickExtract™ DNA Extraction Solution. DNA was extracted from the
following organs using 100 µL of QuickExtract DNA Extraction Solution, and 1 µL of each
extracted sample was used to amplify a single-copy crystallin-like gene. Lane 1, 100-bp
ladder; lanes 2–3, fins; lanes 4–5, eyes; lanes 6–7, scales; lane 8, no-DNA control.
PCR-ready
DNA

Figure 3. The QuickExtract™ DNA Extraction Solution workflow.


Products Size Cat. No.
QuickExtract™ DNA Extraction 50 mL QE09050
Solution (100 Extractions)
5 mL QE0905T
(10 Extractions)

For research or investigational use only.


FM 598408

You might also like