Allele-In-One PCR MasterMix,

pre-mixed solution containing all reagents needed

for PCR and RT-PCR

Description Features
2 X Allele-In-One PCR MasterMix is a pre-mixed solution
containing Taq DNA polymerase, MgCl2, dNTPs, an
inhibitor of Taq at low temperatures, and optimal PCR
RT-PCR Ready: Reverse transcription can be performed in
all Allele PCR mixes by simply adding reverse transcriptase
reaction buffers. The MasterMix contains all necessary
components for PCR; template and primers are not included. Convenient: Reduces time required to assemble PCR
Allele-in-one PCR MasterMix is optimized for consistent use reactions. HQ loading dye included at no additional charge.
in Allele’s LineSilenceTM productions and other routine
PCR amplification. Fast: Only need to add template and primers

The 1.1X Allele-In-One PCR MasterMix is formulated for Scalable: Can be scaled to any reaction volume required
the utmost convenience. It contains the same components
as the 2X Mix except the final concentrations are 1.1X. Add Reliable: Strict quality-control provides consistency and
only PCR primers and template with a combined volume of certainty in our products
roughly 1/10 of the final reaction volume before running the
reactions.
Both 2X and 1.1X Mixes come with pre-added loading
dye at no extra cost. The inert red colored dye does not
interfere with PCR reactions. PCR products can be directly
loaded onto the gel after completion of reactions

Box 1 | Product List

2X MasterMix
ABP-PP-MM02961 96 x 10μl reactions
ABP-PP-MM02965 96 x 50μl reactions

1.1X MasterMix Quality Control

ABP-PP-MM11961 96 x 10μl reactions We test each batch of the enzyme and MasterMix
preparation using various PCR reactions, in comparison
ABP-PP-MM11965 96 x 50μl reactions
with similar products from other sources.
♦Catalog # ABP-PP-MM02961 is included in
Allele-In-One Mouse Tail Direct PCR Kit, available
separately as Catalog # ABP-PP-MT02100 (100 Box 3 | MasterMix Comparison
Rxns) & ABP-PP-MT02500 (500Rxns).
Allele-In-One PCR MasterMix
consistently generates higher amount
of PCR products compared to other
commonly used PCR mixtures.

3kb

1kb
Box 2 | Dose Response
Allele

Competitors

Competitors
Allele

Amplification has low background and

shows dose response. Amplification of a
0.9kb pesT region from 170kb T4 phage
genome with 2X Allele-In-One PCR
MasterMix, at 25, 28,31, and 35 PCR
cycles.

Allele Biotech-Introducing Cost Effectiveness to Research

Recommended Use Additional Features
♦Both 2X and 1.1X MasterMixes come with pre-added
Allele recommends the following assembly for 50μl and loading dye at no extra cost. The inert red colored dye does
10μl reactions using 2X PCR MasterMix: not interfere with PCR reactions.

For 50μl PCR reaction:

2X PCR MasterMix 25μl ♦PCR products can be directly loaded onto gel after
completion of reactions. Pre-aliquoting either 2X or 1.1X
Template DNA 1-10 ng** MasterMix into 96 PCR ready tubes is provided at $20 extra
Forward Primer ~0.5-1μM final** charge. Allele Oligo customers have the option of pre-loading
PCR MasterMix with primers at $0.20 per reaction.
Reverse Primer ~0.5-1μM final**
Fill to 50μl final volume with nuclease-free water.
Proceed to using a thermo-cycler for the PCR reaction.

Notes:
For 10μl PCR reaction: __________________________________________
2X PCR MasterMix 5μl __________________________________________
Template DNA 0.02-2 ng** __________________________________________
__________________________________________
Forward Primer ~0.5-1μM final**
__________________________________________
Reverse Primer ~0.5-1μM final** __________________________________________
Fill to 10μl final volume with nuclease-free water. __________________________________________
Proceed to using a thermo-cycler for the PCR reaction. __________________________________________
__________________________________________
__________________________________________
Allele recommends the following assembly reactions __________________________________________
using 1.1X PCR MasterMix:
__________________________________________
For 27.5μl PCR reaction: __________________________________________
1.1X PCR MasterMix 25μl __________________________________________
Template DNA 1-10 ng** __________________________________________
___________________________________________
Forward Primer ~0.5-1μM final**
Reverse Primer ~0.5-1μM final**
The combined volume of template and primers should be
about 2.5 μl. Do not add nuclease-free water, there is no
need to adjust final reaction volume.
Proceed to using a thermo-cycler for the PCR reaction.

For 10μl PCR reaction: Welcome to Join in the Discussion at Allele’s Network:
1.1X MasterMix is particularly suited for high throughput Allele News:  www.allelebiotech.com/News/
colony screening. Using 10 μl reaction pre-aliquoted in PCR Allele Blog:  www.allelebiotech.com/blogs/
Allele Facebook:  http://www.facebook.com/…
reaction thin-wall tubes, user needs only add primers and use
toothpicks to transfer E. coli cells in colonies to the reaction. Allele Twitter:  http://www.twitter.com/allele_biotech
Allele Homepage:  http://www.allelebiotech.com
Proceed to using a thermo-cycler for the PCR reaction.
**Template and primer concentrations vary based on primer
pair.

Related Products
 Custom Oligo Synthesis  Allele-In-One Mouse Tail Direct PCR System
 We specialize in very long oligos (e.g., 215 nt)  Lysing mouse tail samples for genotyping PCR
and will ship or deliver our oligos within 24hours.  The Mouse Tail Direct PCR Kit includes Allele-in
Our oligo synthesis service offers a large One PCR MasterMix, which is optimized for DNA
variety of synthesis scales, modifications and template prepared with the lysis buffer but can
purifications for our customer’s various needs. also be used for most routine PCR reactions.

Allele Biotech-Introducing Cost Effectiveness to Research