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Primer 3

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hanifisp45

primer alternatif bgal

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Primer 3

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hanifisp45
14 views1 page
primer alternatif bgal

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primer alternatif bgal

Copyright:

© All Rights Reserved
Download as TXT, PDF, TXT or read online from Scribd
Download as txt, pdf, or txt
14 views1 page

Primer 3

Uploaded by

hanifisp45
primer alternatif bgal

Copyright:

© All Rights Reserved
Download as TXT, PDF, TXT or read online from Scribd
Download as txt, pdf, or txt
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Primer Design Report: One Primer

Report Date: 06/19/13

Source molecule: PDAN5-~1.FAS

----- P R I M E R
Sequence:

5260 bps

I N F O --------------------------------------------

5'-GGTTATGTACAGGAACGCAC-3'
Length: 20;
5' Extension:
Composition:

Bp pos: 436
--none-A: 6 C: 4 G: 6 T: 4

---------------------------------------------------------------------------

PRIMER EVALUATION AND CRITERIA SETTINGS:


Criteria
-------------

Criteria Setting
----------------------------

% GC

Min

50,

Max

60

50

YES

Tm C

Min

55,

Max

80

66

YES

No Hairpins

Energy cutoff 0.0 kcal

--

YES

No 3' Dimers
No Dimers

Reject >= 3 matches at 3' end


Reject >= 7 adj homol bases

2
6

YES
YES

No Runs
No 3'GC runs

Reject >= 3 base runs


Reject >= 3 G or C at 3' end

2
1

YES
YES

LARGEST DUPLEXES REPORTED IN PRIMER DIMERS (above)


Primer-dimers with 3'end involvement:
5'-GGTTATGTACAGGAACGCAC-3'
::
3'-CACGCAAGGACATGTATTGG-5'
Other Primer-Dimers:
5'-GGTTATGTACAGGAACGCAC-3'
::::::
3'-CACGCAAGGACATGTATTGG-5'

Actual
------

Meets Criteria
--------------

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