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Preprint CASE REPORT | doi:10.20944/preprints202409.1137.v1

Exploring Dengue Infection in a Vaccinated Individual: Preliminary Molecular Diagnosis and Sequencing Insights

Talita Émile Ribeiro Adelino, Sílvia Helena Sousa Pietra Pedroso, Maurício Lima, Luiz Marcelo Ribeiro Tomé, Natália Rocha Guimarães, Vagner Fonseca, Paulo Eduardo de Souza da Silva, Keldenn Melo Farias Moreno, Ana Cândida Araújo e Silva, Náthale Rodrigues Pinheiro, Carolina Senra Alves de Souza, Luiz Carlos Junior Alcantara, Marta Giovanetti, Felipe Campos de Melo Iani

Subject: Biology And Life Sciences, Virology Keywords: DENV monitoring; next-generation sequencing; genomic surveillance; vaccine breakthrough

Online: 14 September 2024 (11:46:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1110.v1

How Accurately Topsoil Texture Is Shown on Agricultural Soil Maps? A Case Study of Eleven Fields Located in Poland

Michał Stępień, Dariusz Gozdowski, Stanisław Samborski

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: agricultural soil maps; soil texture class; agronomic category; purity; averaged agreement

Online: 14 September 2024 (10:13:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1060.v1

Evaluation of the Antifungal Activity of Eugenol against the Candida albicans Genomic DNA

Danyelle Gomes de Sousa, Francisca Alves dos Santos, Dárcio Luiz de Sousa Júnior, Tássia Thaís Al Yafawi, Irwin Rose Alencar de Menezes, Henrique Douglas Melo Coutinho, Maria Karollyna do Nascimento Silva Leandro

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: cell death; eugenol; fungal DNA

Online: 14 September 2024 (10:08:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1114.v1

Assessing the Pathogenicity of Berkeleyomyces rouxiae and Fusarium oxysporum f. sp. vasinfectum on Cotton (Gossypium hirsutum) Using a Rapid and Robust Seedling Screening Method

Andrew Chen, Duy P. Le, Linda J. Smith, Dinesh Kafle, Elizabeth A. B. Aitken, Donald M. Gardiner

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Upland cotton; pathogenicity; fungal effectors; Fusarium wilt; black root rot; Secreted In Xylem effector genes; endophyte; Translation elongation factor-1 alpha subunit

Online: 14 September 2024 (08:49:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1116.v1

Characterization of Biofilm Formation by Staphylococcus aureus Depending on NaCl Concentration

Yusuke Iwabuchi, Hitoyuki Kato, Masanori Saito, Hidenobu Senpuku

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: biofilm; membrane vesicle; S. mutans; NaCl; S. aureus

Online: 14 September 2024 (08:44:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1109.v1

Intelligibility Sound Therapy Enhances the Ability of Speech-in-Noise Perception and Pre-perceptual Neurophysiological Response

Takashi Ishino, Kei Nakagawa, Fumiko Higashikawa, Sakura Hirokane, Rikuto Fujita, Chie Ishikawa, Tomohiro Kawasumi, Kota Takemoto, Takashi Oda, Manabu Nishida, Yuichiro Horibe, Nobuyuki Chikuie, Takayuki Taruya, Takao Hamamoto, Tsutomu Ueda, Louis Yuge, Sachio Takeno

Subject: Biology And Life Sciences, Life Sciences Keywords: intelligible-hearing (IH) sound; sensory neural hearing loss; speech-in-noise perception; cortical auditory evoked fields (AEF); N1m-P2m amplitude; magnetic mismatch negativity (MMNm)

Online: 14 September 2024 (07:31:09 CEST)

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Aural rehabilitation with hearing aids can decrease the attentional requirements of cognitive resources by amplifying the deteriorated-frequency sound in hearing loss patients and improve the auditory discrimination ability like speech-in-noise perception. As aural rehabilitation with an intelligible-hearing sound also can be hopeful, the aim of this study was to evaluate the effectiveness of the aural rehabilitation with intelligible-hearing sound for hearing loss patients. Adult native Japanese speakers (17 males and 23 females, 68.43±9.23 years) showed over 30 dB at 125, 250, 500, 1000, 2000, 3000, 4000, 8000, 10000, and 12000Hz on any side, were recruited and underwent Mini-Mental State Examination Japanese. we conducted self-evaluation questionnaire for hearing problems of voice, gap detection test, fast speech test, and speech-in-noise test, pure tone audiogram, speech perception test using Japanese 67-S, cortical auditory evoked fields, and magnetic mismatch negativity before and after the non-intelligible-hearing (N=20) and intelligible-hearing (N=20) sound therapy with listening to music for one hour a day for 35 days. Better hearing ear was defined using a four-frequency pure-tone average at the thresholds of 500, 1000, 2000 and 4000 Hz. After the sound therapy, speech-in-noise test with signal to noise ratio +10 in better hearing ear showed significant improvement (p<0.05), and N1m-P2m amplitudes showed the significant increase in Lt superior temporal gyrus at the stimulus from better hearing ear (p<0.05). The significant enhancement of the magnetic mismatch negativity amplitude at Lt superior temporal gyrus was exhibited after the sound therapy (p<0.01). Intelligible-hearing sound therapy can improve the ability of speech-in-noise perception in better hearing ear and the enhancement of central cortex response, which reflect the ability of working memory, was proved by cortical auditory evoked fields and magnetic mismatch negativity. Intelligible-hearing sound therapy can be a valuable aural rehabilitation method for sensory neural hearing loss as same as hearing aids.

Preprint ARTICLE | doi:10.20944/preprints202409.1105.v1

Urban Ecology of Red-Whiskered Bulbul: Feeding Behavior, Breeding Success and Behavioural Patterns in New Barrackpore, West Bengal, India

Md. Abu Imran Mallick, Nirdesha Biswas

Subject: Biology And Life Sciences, Behavioral Sciences Keywords: bird species; behavioral adaption; conservation; foraging strategies; habitat adaption; Pycnonotus jocosus

Online: 14 September 2024 (05:39:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1020.v1

The Effects of Competition on Exercise Intensity and the User Experience of Exercise, during Virtual Reality Bicycling for Young Adults

John L Palmieri, Judith E Deutsch

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Virtual reality; bicycling; aerobic exercise; wearable sensors; visual feedback; competition; eye-tracking; visual attention; motivation; enjoyment

Online: 14 September 2024 (05:29:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1025.v1

Diversity and Evolution of NLR Genes in Citrus Species

Zhiwei Xiong, Wanshan Zhang, Hui Yin, Jiaxing Wan, Zhuozhuo Wu, Yuxia Gao

Subject: Biology And Life Sciences, Horticulture Keywords: NLR genes; citrus; diversity; adaptive evolution

Online: 14 September 2024 (05:26:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1106.v1

Optimized Polyhydroxybutyrate Production by Neobacillus Niacinii GS1 Utilizing Corn Flour, Wheat Bran, and Peptone: A Sustainable Approach

Shrimali Gaurav, Gangawane Ajit, Rami Esha, Shah Hardik, Thumar Kashyap, Deepak Sahoo, Ashish Patel, Jens Ejbye Schmidt

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Polyhydroxybutyrate (PHB); Neobacillus niacinii GS1; Response Surface Methodology (RSM); Biodegradable Plastics; Agricultural Residual; Biopolymer Production

Online: 14 September 2024 (05:25:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1017.v1

Optimal Forest Management Using Multi-Objective and Game Theory Techniques: Considering Environmental and Economic Factors

Neda Amiri, Soleiman Mohammadi Limaei

Subject: Biology And Life Sciences, Forestry Keywords: decision making; multi-objective; Pareto optimum; game theory; Nash equilibrium; epsilon-constrain; net present value; carbon sequestration

Online: 14 September 2024 (05:00:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1090.v1

Pacemaker Optimization Mechanisms in the Spectrum of Cardiac Disease Rationale to Protocol

Philip Houck

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: pacemaker optimization; protocol for spectrum of cardiac disease; Echocardiogram parameters

Online: 14 September 2024 (04:34:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1016.v1

DNA Gene's Basic Structure as a Nonperturbative Circuit Quantum Electrodynamics: Is RNA Polymerase II the Quantum Bus of Transcription?

Raul Riera Aroche, Yveth M. Ortiz García, Esli C. Sánchez Moreno, José S. Enriquez Cervantes, Andrea C. Machado Sulbaran, Annie Riera Leal

Subject: Biology And Life Sciences, Biophysics Keywords: DNA gene's basic structure; circuit quantum electrodynamics; quantum bus of transcription

Online: 14 September 2024 (03:36:16 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1057.v1

Improving Crops Using Omics Databases of Arabidopsis (Arabidopsis Thaliana) and Other Model Plants

Andrés S. Ortiz-Morazán, Marcela María Moncada

Subject: Biology And Life Sciences, Plant Sciences Keywords: Arabidopsis (Arabidopsis thaliana), Bioinformatics, Genetic improvement, Omics databases, Crops.

Online: 13 September 2024 (14:51:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1037.v1

Co-extraction of DNA and RNA from Candida albicans Using a Chemical Method in Conjunction with Silicon Carbide with Few Cells

Elizabeth Cristina Vieira de Freitas, Francisca Alves dos Santos, Maria Raíssa Vieira Lopes, Dárcio Luiz de Sousa Júnior, Tássia Thaís Al Yafawi, Ana Carolina Ferreira Araújo, Priscilla Ramos Freitas, Irwin Rose Alencar de Menezes, Henrique Douglas Melo Coutinho, Maria Karollyna do Nascimento Silva Leandro

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Chloriform-phenol; Extraction; Nucleic acids

Online: 13 September 2024 (11:32:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1082.v1

Effect of Temperature Over the Growth and Biofilm Formation of the Thermotolerant Aspergillus flavus

José Alejandro Hernández-Benítez, Brenda Nallely Santos-Ocampo, Daniel Genaro Rosas-Ramírez, Adrian Ramírez-Granillo, Luis Antonio Bautista-Hernández, Víctor Manuel Bautista-de Lucio, Nestor Octavio Pérez, Aída Verónica Rodríguez-Tovar

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Aspergillus flavus; biofilm; thermotolerant; extracellular matrix; lipid droplets; antifungal susceptibility

Online: 13 September 2024 (11:25:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1039.v1

Dereplication of Tetronic Acid Derivatives Produced by Calea pinnatifida (Asteraceae) Endophytes and Their Antimicrobial Properties

Bianca Barna, Lhaís Araújo Caldas, Jackson Monteiro, Augusto Leonardo Santos, Renata Castiglioni Pascon, Marcelo Afonso Vallim, Marcelo José Pena Ferreira, Sarah Santos Gonçalves, Glaucia Queiroz dos Santos, Anderson Messias Rodrigues, Jamile Ambrósio de Carvalho, Suzan Pantaroto de Vasconcellos, Patricia Sartorelli

Subject: Biology And Life Sciences, Life Sciences Keywords: Calea pinnatifida, Endophytic fungi, Hypomontagnella barbarensis, antimicrobial activity, dereplication.

Online: 13 September 2024 (10:43:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1066.v1

Nitrogen Assimilation, Biomass and Yield in Response to Application of Algal Extracts, Rhizobium sp. and Trichoderma asperellum, as Biofertilizers in Hybrid Maize

Sandra Pérez-Álvarez, Erick H. Ochoa-Chaparro, Julio César Anchondo-Páez, César M. Escobedo-Bonilla, Joel Rascón-Solano, Marco A. Magallanes-Tapia, Luisa Patricia Uranga-Valencia, Reinier Hernández-Campos, Esteban Sánchez

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: algal extracts; biofertilizers; nitrogen assimilation; Rhizobium sp.; Trichoderma asperellum; Zea mays L.

Online: 13 September 2024 (08:46:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1049.v1

Aging of the Neuromuscular System: What Are the Causes of Age-Related Loss of Strength and Muscle Mass, and Can We Prevent Its Development and Progression?

Thomas Gustafsson, Brun Ulfhake

Subject: Biology And Life Sciences, Aging Keywords: dynapenia; muscle fiber atrophy; motor unit; ageing; senescence

Online: 13 September 2024 (08:25:06 CEST)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202409.1063.v1

Fractal Calculus Facilitates Rethinking 'Hard Problems'; A New Research Paradigm

BRUCE West

Subject: Biology And Life Sciences, Other Keywords: Fractal Paradigm; fractal calculus; heart rate variability (HRV); Levy statistics; control hypothesis; sinoatrial node control

Online: 13 September 2024 (08:24:23 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.0714.v3

Analysis of Beyfortus® (Nirsevimab) Immunization Campaign: Effectiveness, Biases, and ADE Risks in RSV Prevention

Helene Banoun

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: nirsevimab; beyfortus; ADE; RSV; bronchiolitis; mAbs; therapeutic monoclonal antibody

Online: 13 September 2024 (08:24:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1051.v1

Antioxidant Activity of Zingiber Officinale R. Extract Using Pressurized Liquid Extraction Method

Marlon Saldaña-Olguin, Bernardo Junior Quispe-Ciudad, Elza Berta Aguirre-Vargas

Subject: Biology And Life Sciences, Food Science And Technology Keywords: antioxidant activity; aqueous extract; ginger; phenolic compounds; pressurized liquid extraction

Online: 13 September 2024 (04:23:54 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0951.v1

The Emerging Role of Long Noncoding RNAs’ in Sorafenib Resistance within Hepatocellular Carcinoma

Puneet Vij, Mohammad Shabir Hussain, Sanjaya Kumar Satapathy, Everardo Cobos, Manish Kumar Tripathi

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Long noncoding RNA (LncRNA); Sorafenib Resistance; Hepatocellular Carcinoma (HCC); EGFR; Autophagy; VEGFA; Proteomics; Renal Cell Carcinoma (RCC)

Online: 13 September 2024 (04:17:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.0680.v2

A Systematic Review of Aphantasia: Concept, Measurement, Neural Basis, and Theory Development

Feiyang Jin, Shen-Mou Hsu, Yu Li

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: aphantasia; visual imagery; mental imagery; vividness; cognitive functioning; mental health

Online: 12 September 2024 (16:01:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1006.v1

Effect of De-Husking on Nutritional and Antioxidant Quality of Nigerian Millet Varieties

Abduljalil Muhammad Mode, Umar Faruk Magaji, Salisu Yusuf Yahaya, Nuhu Ginom Zaki, Shamsudeen Umar Dandare

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Millet; De-husking; Antioxidants; Phytochemicals; Minerals; Vitamins

Online: 12 September 2024 (15:43:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0997.v1

Chemical Markers in Italian Propolis: Chrysin, Galangin and Cape as Indicators of Geographic Origin

Elisabetta Miraldi, Giorgio Cappellucci, Giulia Baini, Elia Silvia Pistone, Marika Allodi, Gabriele Costantino, Chiara Spaggiari, Marco Biagi

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Italian propolis; flavonoids; pinocembrin; chrysin; CAPE; geographical diversity.

Online: 12 September 2024 (15:41:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1030.v1

Unveiling the Nutrient Signatures in Corn (Zea Mays L.) Grains: A Pivotal Indicator of Yield Potential

Nour Ismail, Lotfi Khiari, and Rachid Daoud

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Nutrient Diagnosis Norms (NDN), Nutrient signature, Global nutrient imbalance index (GNII), High yielding subpopulation

Online: 12 September 2024 (15:41:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0928.v1

Two Forms of Thick Filament in the Flight Muscle of Drosophila melanogaster

Hosna Rastegarpouyani, Alimohammad Hojjatian, Kenneth A. Taylor

Subject: Biology And Life Sciences, Biophysics Keywords: ageing; flightin; myofilin; paramyosin; stretchin-klp

Online: 12 September 2024 (15:29:31 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202409.0995.v1

Knee Joint Response to Mechanical Loading: Bounding Mechanotransduction with Rehabilitation

Bartłomiej Kacprzak, Mikołaj Stańczak

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: knee joint; molecular biology; cell biology; mechanotransduction

Online: 12 September 2024 (12:56:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0922.v1

Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend

Nicholas L.F. Gallina, Nicole I Tardi, Xilin Li, Alvin Cai, Mandy Horn, Bruce Applegate, Lavanya Reddivari, Arun K. Bhunia

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Probiotics; biofilm; antimicrobial activity; adhesion; pathogens; canine gut health; anti-inflammatory response

Online: 12 September 2024 (11:26:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0933.v1

Congenital Radioulnar Synostosis Review: Recommendations and Treatment Outcomes

Sergi Alabau-Rodríguez, Jose Felix Garrido Ferrer, Xavier Mir-Bulló, Lidia Ana Martin Dominguez, Albert Pardo Pol, Francisco Soldado Carrera

Subject: Biology And Life Sciences, Life Sciences Keywords: radioulnar synostosis; radius; ulna; congenital

Online: 12 September 2024 (11:12:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0964.v1

Roseto Revisited: Fact or Fiction

Samrachana Adhikari, Olugbenga Ogedegbe, Orrin Devinsky

Subject: Biology And Life Sciences, Life Sciences Keywords: diet; obesity; health; social support; saturated fat

Online: 12 September 2024 (11:07:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0996.v1

Effect of Citrus Fruit Constructions on Gas Diffusion Resistance and Internal Gas Concentration of Oxygen and Carbon Dioxide for Various Cultivars of Citrus Fruits

Morimatsu Kazuya, Konagaya Keiji

Subject: Biology And Life Sciences, Horticulture Keywords: citrus fruit; gas diffusion resistance; respiration; internal gas concentration

Online: 12 September 2024 (10:57:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0926.v1

Genome Sequencing Reveals the Potential of Enterobacter sp. strain UNJFSC003 for Hydrocarbon Bioremediation

Gianmarco Castillo, Sergio Eduardo Contreras-Liza, Carlos I. Arbizu, Pedro Manuel Rodriguez-Grados

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: NGS; genomics; microbiology; benzo[a]pyrene; hydrocarbon and bioremediation; Molecular Docking

Online: 12 September 2024 (10:49:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0987.v1

3D FEM Modeling of Polish Apple ‘Chopin’: Elastic vs. Elastoplastic Behavior Across Maturity Stages

Monika Słupska, Roman Stopa

Subject: Biology And Life Sciences, Food Science And Technology Keywords: discrete model; FEM; apple; fruit; stage of ripening

Online: 12 September 2024 (09:23:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0984.v1

Development of a Sensitive and Reliable Immunoassay to Quantify TDP-43 in Human Biofluids

Jiyan An, Lathika Gopalakrishnan, Vanessa Ortega, Justin Saul, Renu Kadali, Robert Bowser

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: TDP-43; immunoassay; MSD; ALS; biomarker; neurodegenerative disease

Online: 12 September 2024 (09:16:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0954.v1

Characterization of MSC Growth, Differentiation, and EV Production in CNF Hydrogels under Static and Dynamic Cultures in Hypoxic and Normoxic Conditions

Ilias Nikolits, Farhad Chariyev-Prinz, Dominik Egger, Falk Liebner, Nicolas Mytzka, Cornelia Kasper

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: mesenchymal stem cells; cellulose; hydrogels; hypoxia; extracellular vesicles; immunomodulation

Online: 12 September 2024 (08:18:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0958.v1

Assessment of Bacteriological Quality of Drinking Water in Taiz and Ibb Governorates, Yemen

Ali Ahmed Al-Hadheq, Wadhah Edrees, Bashir Al-Ofairi

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Escherichia coli; ; Coliform; Total Bacterial Count; Presumptive Test; Taiz; Ibb; Yemen

Online: 12 September 2024 (08:16:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0914.v1

Timing and Graded BMP Signalling Determines Fate of Neural Crest and Ectodermal Placode Derivatives from Pluripotent Stem Cells

Keshi Chung, Malvina Millet, Ludivine Rouillon, and Azel Zine

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: bone morphogenetic proteins; human pluripotent stem cells; human cell models; organoids; preplacodal ectoderm; otic lineage

Online: 12 September 2024 (07:41:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0963.v1

CBD-X Extracts in Asthma Management: Reducing Th2-Driven Cytokine Secretion and Neutrophil/Eosonophil Activity

Miran Aswad, Antonina Pechkovsky, Narmeen Ghanayiem, Yaniv Dotan, Igal Louria-Hayon

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: CBD extracts; asthma; type 2 helper T cells (Th2); neutrophil; Eosonophil; OVA-Induced Asthmatic mouse model; cytokines; migration; IgE

Online: 12 September 2024 (05:35:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0935.v1

Changes in Gut Microbiota in a Peruvian Cattle Genetic Nucleus by Breed and Correlations with Beef Quality

Carlos Quilcate, Richard Estrada, Yolanda Romero, Diorman Rojas, Rolando Mamani, Renán Dilton Hañari-Quispe, Mery Aliaga, Walter Galindo, Héctor V. Vásquez, Jorge L. Maicelo, Carlos I. Arbizu

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: gut microbiota; cattle breeds; microbial diversity; meat quality; hematological parameters; host genetics; 16S/18S rRNA

Online: 12 September 2024 (03:28:35 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202409.0918.v1

Endothelin-1 as an Activator of Pro-inflammatory Microglia Cells

Yaritza Inostroza-Nieves, Shakira Bou, Jose Alvarado-Perez, Diego Capo-Ruiz, Jessica Garcia, Jean P Moliere, Claudia P Arenas

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: endothelin; inflammation; microglia; multiple sclerosis; reactive oxygen species

Online: 11 September 2024 (17:10:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0870.v1

Prenatal Exposure to a Human Relevant Mixture of EDCs Affects Mandibular Development in Mice

Vagelis Rinotas, Antonios Stamatakis, Athanasios Stergiopoulos, Carl-Gustaf Bornehag, Joëlle Rüegg, Marietta Armaka, Efthymia Kitraki

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: EDCs; mandible; mice; micro-CT

Online: 11 September 2024 (17:08:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0859.v1

Comparing the Performance of Automatic Milking Systems through Dynamic Testing Also Helps to Identify Potential Risk Factors for Mastitis

Stefano Milanesi, Dario Donina, Viviana Chierici Guido, Francesca Zaghen, Valerio Massimo Sora, Alfonso Zecconi

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: milking machine; automatic milking system; milking dynamic test; bimodality; mastitis

Online: 11 September 2024 (16:59:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0907.v1

PIM Kinases Regulate Estrogen Receptor α Signaling in Luminal A Breast Cancer Cells

William B. Eccleshall, Kwan Long Mung, Iida-Maria Laiho, Cecilia M. Sahlgren, Päivi J. Koskinen

Subject: Biology And Life Sciences, Life Sciences Keywords: PIM kinases; estrogen receptor α; luminal A breast cancer; targeted therapy resistance

Online: 11 September 2024 (16:23:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0901.v1

Comprehensive Phylogenetic and Immunogenic Analysis of SARS-CoV-2 and Related Coronaviruses

Emmanuel Owusu Ansah, Kwadwo Fosu, Theophilus Abonyi Mensah, Emmanuel Plas Otwe, Daniel Sakyi Agyirifo

Subject: Biology And Life Sciences, Virology Keywords: Coronavirus; SARS-CoV-2; spike protein; phylogenetics; CTL epitope; antigenic

Online: 11 September 2024 (15:28:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0912.v1

Ellagic Acid Mitigates Cadmium Exposure-Induced Apoptosis in HT22 Cells via Inhibiting Oxidative Stress, Mitochondrial Dysfunction, and Activating Nrf2/Ho-1 Pathway

Yue Liu, Chunhong Chen, Zhihui Hao, Jianzhong Shen, Shusheng Tang, Chongshan Dai

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cadmium; Ellagic acid; JNK pathway; Oxidative stress; Apoptosis; Nrf2/HO-1 pathway

Online: 11 September 2024 (14:12:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0825.v1

Ripening Stage in Mechanical Properties of Chopin Apples for FEM Model Construction

Monika Słupska, Roman Stopa

Subject: Biology And Life Sciences, Food Science And Technology Keywords: surface pressure; Micro-Ct; flesh cracking; model validation; fruit

Online: 11 September 2024 (12:34:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0833.v1

Exploring Lignin Biosynthesis Genes in Rice: Evolution, Functions, and Expressions

Munsif Ali Shad, Xukai Li, Muhammad Junaid Rao, Zixuan Luo, Xianlong Li, Aamir Ali, Lingqiang Wang

Subject: Biology And Life Sciences, Plant Sciences Keywords: rice; lignin monomers; gene families; cell wall; gene expression

Online: 11 September 2024 (12:32:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0892.v1

Revisiting Avian Metapneumovirus Subtype B in Broiler Chickens and Turkeys in Morocco: First Molecular Characterization

Amine Mernizi, Abdeljelil Ghram, Hicham Fathi, Enrique Carceller Sebastiá, Juan-Luis Criado, Martina Dardi, Oumayma Arbani, Mohammed Bouslikhane, Mohammed Mouahid, Caterina Lupini, Elena Catelli, Siham Fellahi, Saadia Nassik

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Avian Metapneumovirus; Subtype B; Morocco; characterization; sequencing; phylogenetic analysis

Online: 11 September 2024 (11:24:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0877.v1

Scalable Ligand-Receptor Binding Affinity Landscape: A Case Study with Ziconotide and Ca_V2.2

Wei Li

Subject: Biology And Life Sciences, Biophysics Keywords: Ligand-Receptor Binding Affinity; ziconotide; CaV2.2; Structural Biophysics; Peptide Design

Online: 11 September 2024 (10:58:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0883.v1

Development of Elite Mother Palms from the Best Performing Slow Vertical Growth Oil Palm (Elaeis guineensis Jacq.) Genotypes

Anitha Pedapati, Suresh K, Mathur RK, Ravichandran G, Naveen Kumar P, Bhagya H.P, Kalyana Babu B, Shankar Narayana K

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Genotypes; dura; oil palm; dwarf; mother palms

Online: 11 September 2024 (10:45:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0824.v1

Depicting of DNA Makeup of Spring Bread Wheat Genotypes Using SSR Markers and Their Association with Heat Tolerance

Md. Forhad Amin, Mohammad Nur Alam, Golam Faruq, Md. Mustafa Khan, Md. Mahbubur Rahman

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Bread wheat; Fingerprinting; Genetic diversity; Heat tolerance; SSR marker

Online: 11 September 2024 (10:39:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0848.v1

High-Intensity Interval Training in Different Slopes on Aerobic Performance: A Randomized Controlled Trial

Alberto Souza Sá Filho, Roberto Dib Bittar, Pedro Augusto Inacio, Marcelo Magalhães Sales, Júlio Brugnara Mello, Iransé Oliveira-Silva, Patricia Sardinha Leonardo, Gaspar Rogério Chiappa, Rodrigo Brandão Lopes-Martins, Tony Meireles Santos

Subject: Biology And Life Sciences, Other Keywords: Anaerobic Threshold; Running Velocity; Exercise Test; Endurance

Online: 11 September 2024 (10:36:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0851.v1

Comparison of Ultra-High Pressure Cold Brew Coffee with Conventional Cold Brew Coffee at Different Roasting Degrees: Physicochemical Characteristics, Non-Volatile Compositions and Volatile Compositions

Qihan Shi, Ying Xiao, Yiming Zhou, Wenxiao Tang, Feng Jiang, Xiaoli Zhou, Hongxiu Lu

Subject: Biology And Life Sciences, Food Science And Technology Keywords: cold brew; ultra-high pressure (UHP); roasting degree; volatile component; non-volatile component; sensory evaluation; orthogonal partial least squares-discriminant analysis (OPLS-DA)

Online: 11 September 2024 (10:27:54 CEST)

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Previous research has indicated that ultra-high pressure (UHP) technology is useful in speeding up the extraction process of cold brew (CB) coffee. However, the impact of different degrees of roasting on UHP CB coffee is still unclear. Therefore, this study aimed to investigate the effects of three different degrees of roasting (light, medium, and dark) on the physicochemical and flavor characteristics of UHP CB coffee. The results of this study showed that most physicochemical characteristics of UHP coffee were similar to those of conventional CB coffee, regardless of the degree of roasting. However, the majority of physicochemical characteristics, non-volatile components, and sensory evaluation significantly increased with an increase in roasting degree. The HS-SPME-GC/MS analysis showed that most volatile components, such as pyrazines, furans (except furfural), esters, pyrrole, pyridine, and most aldehydes, increased from light to dark roasting, and this was significantly evident in CB coffee compared with UHP. The content of volatile components in UHP coffee was significantly (P<0.05) higher than that in CB coffee at the same degree of roasting, especially with dark roasting. The combination of orthogonal partial least squares-discriminant analysis (OPLS-DA) and principal component analysis (PCA) indicated that the distance between coffee samples at different degrees of roasting under CB coffee was greater than that under UHP coffee. However, the distance between these two extraction methods at medium roasting was evidently closer than that at light and dark roasting. Moreover, 15 representative differential compounds, such as were Hazelnut pyrazine, linalool, butane-2,3-dione, 3- methylbutanal found by the calculation of odor-active values (OAVs) indicating the main contributing compounds to odor. Overall, the effect of roasting degrees on UHP coffee was less than that on CB coffee, which was consistent with the results of physicochemical characteristics, volatile components, and sensory evaluation.

Preprint ARTICLE | doi:10.20944/preprints202409.0852.v1

Physiological and Transcriptomic Levels Reveal the Response Mechanism of Maize to Deep Sowing and the Mechanism of Exogenous MeJA to Alleviate Deep Sowing Stress

Fang Wang, Zhi Jin Feng, Xin Yi Yang, Guang Kuo Zhou, Yun Ling Peng

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Maize; Exogenous methyl jasmonate; Deep sowing stress; Mitigation effect

Online: 11 September 2024 (10:16:00 CEST)

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Drought and water scarcity are significant factors limiting the development of China's maize industry. Deep sowing, as a method to mitigate drought and preserve soil moisture and seedlings, can effectively mitigate the adverse effects of drought stress on their growth. However, deep sowing can influence the emergence of maize seedlings and has a certain impact on their growth and development. To investigate the physiological and transcriptomic changes in maize inbred lines under deep sowing stress, and the alleviation effects of exogenous hormones, this study investigated the response of mesocotyl in deep-seeding tolerant maize inbred line Qi319 and deep-seeding sensitive maize inbred line Zi330 to deep-seeding stress under exogenous MeJA treatment. The physiological and transcriptomic mechanisms underlying the alleviation of deep-seeding stress by exogenous MeJA were elucidated. Results showed that compared to normal sowing depth, mesocotyl length (MES), mesocotyl and coleoptile length (MESCOL) significantly increased under deep-seeding stress for both inbred lines, while seedling length (SDL) was significantly inhibited. However, after adding 1.5 μmol·L^-1 MeJA, MES, MESCOL, and SDL significantly increased compared to deep-seeding stress alone. Exogenous MeJA promoted cell elongation, enhanced mesocotyl length, and improved seedling emergence ability. RNA-Seq sequencing revealed differentially expressed genes (DEGs). Under deep-seeding stress compared to normal sowing depth conditions, 1248 DEGs were identified in Qi319 and 1288 DEGs were identified in Zi330. After adding exogenous MeJA under deep-seeding stress compared to normal sowing depth with exogenous MeJA treatment conditions, 538 DEGs were identified in Qi319 and 2284 DEGs were identified in Zi 330. GO enrichment analysis showed that differentially expressed genes were mainly concentrated in biological processes, cell components and molecular functions. KEGG enrichment analysis showed that differentially expressed genes were mainly concentrated in transport and catabolism, signal transduction, amino acid metabolism and lipid metabolism processes. Compared with the deep-seeded treatment, 12 differentially expressed genes were co-expressed in the two inbred lines under exogenous MeJA treatment, and 10 key genes, including ornithine decarboxylase, terpene synthase 7 and ethylene reaction transcription factor 11, were screened out. Our findings elucidate the molecular mechanism underlying maize's tolerance response to deep seeding and offer valuable insights into candidate gene resources for improving deep seeding tolerance in maize.

Preprint ARTICLE | doi:10.20944/preprints202409.0836.v1

Early Stage Infection-Specific Heterobasidion annosum (Fr.) Bref. Transcripts in the H. annosum – Pinus sylvestris L. Pathosystem

Maryna Ramanenka, Dainis Edgars Ruņģis, Vilnis Šķipars

Subject: Biology And Life Sciences, Forestry Keywords: plant pathology; root rot; pathogen gene expression; early stage infection

Online: 11 September 2024 (09:41:02 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202408.1271.v2

Bioactive Factors Isolated and Purified from Bovine Colostrum Can Restore Extracellular Matrix under Degradation by Metalloproteinases

Federica Coppa, Graziella Giuffrida, Giulia Iannello, Stefania Pennisi, Greta Ferruggia, Maria Violetta Brundo

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: mechanical signals; wound healing; collagen; integrin; mechanotransducer; ELISA test

Online: 11 September 2024 (03:46:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0758.v1

The Current State of Cell Viability Methods

Eneko Madorran

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cell viability; cell-based methods; in vitro toxicology

Online: 11 September 2024 (03:44:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0772.v1

Commercialized “Smudge Sticks” Used as Incense in the Netherlands: An Inventory of Plants and Trends behind a New Age Fashion

Isabela Pombo Geertsma, Berber E. Zandstra, Anastasia Stefanaki, Tinde R. van Andel

Subject: Biology And Life Sciences, Plant Sciences Keywords: Ethnobotany; cultural appropriation; Native American; Europe; California; United States; ritual plants; Neo Paganism; Wicca; witchcraft

Online: 11 September 2024 (03:36:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0750.v1

Down Syndrome in Children: An Inborn Error of Immunity with Immune Dysregulation

Aleksandra Szczawińska-Popłonyk, Natalia Popłonyk, Karina Awdi

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: down syndrome; immunodeficiency; immune dysregulation; inborn error of immunity; regulatory T cells

Online: 10 September 2024 (15:16:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0748.v1

In Vitro Neuroendocrine Differentiation of BPH-1 Cells under β2 Adrenergic Receptor Stimulation

Natascha Pigat, Laura Nalleli Garrido Castillo, Coline Lefèvre, Manon Baures, Vincent Goffin, Julien Anract, Anne-Sophie Armand, Thierry Capiod

Subject: Biology And Life Sciences, Life Sciences Keywords: beta blockers; prostate; AMACR; Synaptophysin; kscipline

Online: 10 September 2024 (15:10:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0808.v1

Gut Microbiome and Metabolome Alterations in Overweight or Obese Adult Population after Weight-Loss Bifidobacterium brevis BBr60 Intervention: A Randomized Controlled Trial

Zhouya Bai, Ying Wu, Dejiao Gao, Yao Dong, Yujia Pan, Shaobin Gu

Subject: Biology And Life Sciences, Life Sciences Keywords: Bifidobacterium brevis BBr60; overweight or obese; randomized controlled trial; gut microbiota; metabolic profile

Online: 10 September 2024 (13:38:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0816.v1

Farmers’ Perception Assessment of Water and Agronomic Management in Rice Irrigation Schemes in Togo

Alfassassi Arouna, Israel K. Dzomeku, Abdul-Ganiyu Shaibu, Abdul Rahman Nurudeen

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: evaluation; agronomic practices; irrigation water management; rice irrigation schemes; Togo

Online: 10 September 2024 (13:22:20 CEST)

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This paper assessed rice farmers' perceptions of agricultural and irrigation practices through their choices. A survey was carried out among 278 rice farmers on five irrigation schemes in three agro-ecological zones of Togo, viz. the savannah region, the forest region and the coastal region. The sample of random respondents was selected from a list of rice farmers provided by the managers of these irrigated schemes. Data collected through face-to-face interviews using kobocollect software were analyzed with R 4.2 software, and the Pearson test was validated at the 5% level. The results showed that the socio-economic characteristics of the rice growers, such as access to agricultural credit, experience, non-agricultural activities, membership of a rice growers' cooperative, gender, level of education and training in growing irrigated rice, and farm labor, significantly influenced the choice of agronomic practices and irrigation management. Characteristics such as experience, training and hired farm labor enhanced paddy yields, while access to agricultural credit was unproductive. Chemical clearing combined with stump removal, tiller ploughing, transplanting and high plant density are the agricultural practices that increased paddy yields, while tiller mudding and basal mineral fertilization were inefficient. In the same way, water distribution by demand, water depth above the soil of less than 5 cm, use of water-saving technologies and farmer's knowledge of how to improve water and fertilizer use-efficiency also helped to boost paddy yields. For sustainable rice production in the context of labor shortages and the financial resources required, future studies will focus on the effectiveness of motorized mudding and agricultural credits for rice farmers. Furthermore, considering the crucial role of training in the adopting of good agricultural and irrigation practices, future research will study strategies for wide diffusion of knowledge to rice producers.

Preprint REVIEW | doi:10.20944/preprints202409.0715.v1

Detrimental Effects of Abiotic Stress on Wheat and Its Management Techniques

Mohammad Nur Alam, Md. Zaherul Islam, Murad Ahmed Farukh, Zhulong Chan, Mst. Masuma Akhter, Md. Tanvir Abedin, Md. Monoar Hossain

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Drought; Heat; Genes; Antioxidants; Salinity; Oxidative stress

Online: 10 September 2024 (12:54:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0749.v1

Path Analysis of Agro Technological Traits Associated with Sugar Yield in Different Sugar Beet (Beta vulgaris L.) Monogerm Cultivars

Farhang Movloudi, Esmail Nabizadeh, Heydar Azizi, Reza Afshar, Saman Yazdanseta, Masoud Hashemi

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Cause and effect relationships; Genetic correlation; Sugar Beet Final yield

Online: 10 September 2024 (12:06:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0722.v1

Benchmarking of DNA Extraction Methods for Metagenomics, 16S rRNA Databases and Assessment of the Skimmed Milk Flocculation for Bacterial Enrichment for Metagenomics from Water Samples

Deyan Donchev, Ivan Stoikov, Antonia Diukendjieva, Ivan N. Ivanov

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: skimmed milk flocculation; vacuum filtration; bacterial metagenomics; benchmarking; DNA extraction; water samples; taxonomic composition; MIQ score; mock standard

Online: 10 September 2024 (10:08:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0725.v1

Dominant Tree Species and Litter Quality Govern Fungal Community Dynamics during Litter Decomposition

Wen-jing Meng, Lin Chang, Zhao-lei Qu, Bing Liu, Kang Liu, Yue-mei Zhang, Lin Huang, Hui Sun

Subject: Biology And Life Sciences, Forestry Keywords: litter decomposition; enzyme activity; fungal community function; nutrient cycling; forest types

Online: 10 September 2024 (10:03:09 CEST)

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Litter decomposition is a crucial biochemical process regulated by microbial activities in forest ecosystem. However, the dynamic response of fungal community during litter decomposition to vegetation changes is not well understood. Here, we investigated the litter decomposition rate, extracellular enzyme activities, fungal community, and nutrient cycling-related genes in leaf and twig litters over a three-year decomposition period in a pure Liquidamabar formosana forest and a mixed L. formosana / Pinus thunbergii forest. The result showed that during the three-year decomposition, twig litter decomposed faster in the mixed forest than that in the pure forest. In both leaf litter and twig litter, β-cellobiosidase, N-acetyl-glucosamidase exhibited higher activities in the mixed forest, whereas phosphatase, β-glucosidase and β-xylosidase were higher in the pure forest. The fungal α-diversity were higher in both litters in the pure forest compared to mixed forest, with leaf litter showing higher α-diversity than twig litter. Fungal species richness and α-diversity within leaf litter increased as decomposition progressed. Within leaf litter, Basidiomycota dominated in the mixed forest while Ascomycota dominated in the pure forest. Funguild analysis revealed that symbiotroph and ectomycorrhizal fungi were more abundant in the mixed forest compared to the pure forest. In the third-year decomposition, genes related to phosphorus cycling was most abundant in both forests, with the pure forest having a higher abundance of cex and gcd genes. Fungal community structure, predicted functional structure, and gene composition differed between the two forest types and between the two litter types. Notably, the fungal functional community structure during the first-year decomposition was distinct from that in the subsequent two-year. These findings suggest that both dominant tree species, litter quality and decomposition time significantly influence the litter decomposition by recruiting different fungal community, thereby affecting the entire decomposition process.

Preprint REVIEW | doi:10.20944/preprints202409.0717.v1

Rethinking Freshwater Cage Aquaculture: The Case of Ghana

Philip Kwasi Banini, Kofi Ferni Anyan, Jacob Zornu, Mabel Ackah, David Narteh Batsa, Kwasi Issifu, Abigail Amankwah, Shimaa E. Ali, Samuel Addo, Kofitsyo S. Cudjoe

Subject: Biology And Life Sciences, Aquatic Science Keywords: Lake Volta; cage aquaculture; mapping inland areas; law enforcement; best management practices; collaboration and capacity building; emergency preparedness

Online: 10 September 2024 (10:00:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0790.v1

Synergistic Dual Targeting of Thioredoxin and Glutathione Systems Irrespective of p53 in Glioblastoma Stem Cells

Fatemeh Jamali, Katherine Lan, Paul Daniel, Kevin Petrecca, Siham Sabri, BASSAM ABDULKARIM

Subject: Biology And Life Sciences, Life Sciences Keywords: Glioblastoma; Glioblastoma stem cells (GSCs); Auranofin; thioredoxin reductase; oxidative stress; glutathione (GSH); reactive oxygen species (ROS); piperlongumine, antioxidant; drug repurposing

Online: 10 September 2024 (09:50:35 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0781.v1

Utilising Plant Growth-Promoting Rhizobacteria (PGPR) to Advance Sustainable Agriculture

Piao Yang, Abraham Condrich, Sean Scranton, Camina Hebner, Ling Lu, Muhammad Azam Ali

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: PGPR; IPM; Sustainable agriculture; Biofertilizer; Biopesticide; Plant fitness tetrahedron

Online: 10 September 2024 (09:39:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0739.v1

Inorganic Polyphosphate Promotes Colorectal Cancer Growth via TRPM8 Receptor Signaling Pathway

Valentina Arrè, Francesco Balestra, Rosanna Scialpi, Francesco Dituri, Rossella Donghia, Sergio Coletta, Dolores Stabile, Antonia Bianco, Leonardo Vincenti, Salvatore Fedele, Chen Shen, Giuseppe Pettinato, Maria Principia Scavo, Gianluigi Giannelli, Roberto Negro

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Colorectal cancer; inorganic polyphosphate; TRPM8 receptor; organoids; ccnb1; proliferation

Online: 10 September 2024 (09:01:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0736.v1

Discovery of Gibellula floridensis from Infected Spiders and Analysis of the Surrounding Fungal Entomopathogen Community

Ross A. Joseph, Abolfazl Masoudi, Mateo J Valdiviezo, Nemat O Keyhani

Subject: Biology And Life Sciences, Parasitology Keywords: spider; insect and spider fungal pathogens; Gibellula; habitat; amplicon sequencing

Online: 10 September 2024 (09:01:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0747.v1

Impact of Tube Additives on Baseline Cell-Free DNA, Blood Nuclease Activity, and DNA Degradation in Serum and Plasma Samples

Gustavo Barcelos Barra, Ticiane Henriques Santa Rita, Rafael Henriques Jacomo, Lídia Freire Abdalla Nery

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cell-Free DNA; Serum; EDTA Plasma; Heparin Plasma; Citrate Plasma; Nuclease Activity; DNase Activity.

Online: 10 September 2024 (09:00:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0741.v1

The Effects of swnK Gene Function of Endophytic Fungi Alternaria oxytropis OW 7.8 on its Swainsonine Biosynthesis

Yinzhe Li, Fan Yang, Ping Lu, Weifu Wang, Li Zhu, Le Kai, Lu Bai

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Alternaria oxytropis OW 7.8; swainsonine; swnK gene; biosynthesis pathway

Online: 10 September 2024 (09:00:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0756.v1

PTSD Increases Risk for Hypertension Development Through PVN Activation and Vascular Dysfunction in Sprague Dawley Rats

Xinqian Chen, Xin Yan, Chunxiu Yu, Qing-Hui Chen, Lanrong Bi, Zhiying Shan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: posttraumatic stress disorder; single prolonged stress; paraventricular nucleus; oxidative stress; vascular dysfunction; vascular inflammation; hypertension

Online: 10 September 2024 (08:54:37 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202409.0723.v1

Barley Seed Germination and Seedling Growth Responses to PEG-Induced Drought Stress

Matthew Davidson-Willis, Guoqi Wen, Bahram Samanfar, Raja Khanal

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: barley; drought stress; seed germination

Online: 10 September 2024 (08:12:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0699.v1

Activated Metabolic Transcriptional Program in Tumor Cells from Hepatoblastoma

Claudia Monge, Raquel Francés, Agnes Marchio, Pascal Pineau, Christophe Desterke, Jorge Mata-Garrido

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: hepatoblastoma; epigenetics; one-carbon; metabolism; cancer; DNA methylation

Online: 10 September 2024 (03:36:54 CEST)

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(1) Background: Hepatoblastoma is the most common primary liver malignancy in children. During its development, metabolic reprogramming becomes particularly relevant due to the liver's intrinsic metabolic functions. Enhanced glycolysis, glutaminolysis, and fatty acid synthesis have been implicated in the proliferation and survival of hepatoblastoma cells. In this study we screened altered over expression of metabolic enzymes in hepatoblastoma tumors at tissue and single cell level. A hepatoblastoma tumor expression metabolic score was established and validated by machine learning; (2) Methods: Starting from Mammalian Metabolic Enzyme Database, bulk RNA-sequencing from GSE104766 dataset was investigated by supervised analyses (tumors versus adjacent liver tissue). Overexpressed enzymes in hepatoblastoma tumors were functionally enriched on KEGG metabolic database to draw a metabolic network with Cytoscape. Activated metabolic markers were used to compute a single-cell metabolic score in human and PDX hepatoblastoma samples from GSE180665 dataset (sc-RNAseq). ROC and area under curve (AUC) were computed on metabolic score. Elasticnet model tuning for metabolic marker expression was performed with r-caret on single cell transcriptome and revealed importance of individual marker to predict tumor cell status; (3) Results: Differential expression analysis on bulk transcriptome identified 287 significantly regulated enzymes between tumor and adjacent liver tissues: 59 of them were found overexpressed in tumors. 45 of the 59 over expressed enzymes were recognized in KEGG database which highlighted a main metabolic network enriched in amino acid metabolism but also carbohydrate, steroid, one carbon, purine, and glucoaminoglycan metabolisms. Based on expression of the 59 over expressed enzymes, the single cell metabolism score computed on GSE180665 dataset allowed to predict tumor cell status with and AUC of 0.98 (sensibility 0.93, specificity: 0.94). Elasticnet model tuned at 0.97 of AUC on individual marker single cell expression ranked top tumor predictive markers by decreasing importance: FKBP10, ATP1A2, NT5DC2, UGT3A2, PYCR1, CKB, GPX7, DNMT3B, GSTP1, and OXCT1; (4) Conclusion: An activated metabolic transcriptional program potentially affecting epigenetic functions was observed by bulk RNAseq in tumors of hepatoblastoma and confirmed at single cell levels in tumor cells.

Preprint REVIEW | doi:10.20944/preprints202409.0687.v1

Elucidating the Pathophysiological Mechanisms of Alzheimer’s Disease

Paula Denisa Saragea

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Alzheimer; neurodegeneration; dementia; β -amyloid plaques; neurofibrillary tangles

Online: 10 September 2024 (03:28:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0712.v1

Prevalence and Genetic Analysis of Porcine Circovirus Type 2 That Circulated between 2014 and 2021 in Jiangsu, China

Qi Xiao, Meng Qu, Jianping Xie, Cigen Zhu, Yuping Shan, Aihua Mao, Wenxian Qian, Jiaping Zhu, Jiahui Guo, Dong Lang, Jiaqiang Niu, Libin Wen, Kongwang He

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: porcine circovirus type 2; Jiangsu Province; genetic analysis; genotype

Online: 9 September 2024 (23:42:53 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0710.v1

Nutritional Values of Wheat and the Roles and Functions of Its Compositions in Health

Md. Zaherul Islam, Mohammad Nur Alam, Mohammad Mokhlesur Rahman, Mohammad Zaherul Islam, Aminur Rahman

Subject: Biology And Life Sciences, Plant Sciences Keywords: Gluten proteins; Nutritional quality; Vitamins and minerals; Wheat grain

Online: 9 September 2024 (16:45:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0657.v1

Unraveling the Genomic Landscape and Stress Responses of Cowpea Thaumatin-Like Proteins: A Comprehensive Analysis

Carolline de Jesús-Pires, José Ribamar Costa Ferreira-Neto, Roberta Lane de Oliveira-Silva, Jéssica Barboza da Silva, Manassés Daniel da Silva, Antonio Felix da Costa, Ana Maria Benko-Iseppon

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: PR-5 gene family; osmotin-like; TLPs; Vigna unguiculata; zeamatin

Online: 9 September 2024 (14:07:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0689.v1

Evaluating Changes in the Local Protein Physicochemical Environment induced by Molecular Dynamics Simulation

Chuyi Wang, Khalid Mahmood, Daniel Park

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: molecular dynamics simulation; protein; single amino acid substitution; protein local physicochemical environment; tensors; variant effect prediction; FireProtDB

Online: 9 September 2024 (12:43:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0569.v1

A Leaky Deep Intronic Splice Variant in CLRN1 Is Associated With Non-syndromic Retinitis Pigmentosa

Maria Abu Elasal, Samer Khateb, Daan M. Panneman, Susanne Roosing, Eyal Banin, Dror Sharon, Asodu Sandeep Sarma

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: CLRN1; deep intronic; inherited retinal diseases; pseudo-exon; retinitis pigmentosa; splicing

Online: 9 September 2024 (12:40:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0625.v1

Cross-task Differences in Cortical Activations for Dynamic Balance in Neurotypical Adults

Robert D. Magruder, Komal K Kukkar, Jose L Contreras-Vidal, Pranav J Parikh

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: balance; perturbation; sway reference; supplementary motor area; cTBS; EEG

Online: 9 September 2024 (12:28:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0593.v1

Qualitative and Quantitative Sex-Related Differences in the Perception of Single Molecules from Coffee Headspace

Giorgia Sollai, Paolo Solari, Roberto Crnjar

Subject: Biology And Life Sciences, Food Science And Technology Keywords: smell; GC-O technique; VARUs intensity; gender; individual variability

Online: 9 September 2024 (12:25:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0685.v1

Effects of Substituting Fishmeal with Soy Protein Concentrate Supplemented with Essential Amino Acids in the Olive Flounder (Paralichthys olivaceus) Diet on the Expression of Growth, Stress, Immune, and Digestive Enzyme-Related Genes

Sang Hyun Lee, Jong-Won Park, Minhwan Jeong, Dain Lee, Julan Kim, Hyun-Chul Kim

Subject: Biology And Life Sciences, Aquatic Science Keywords: fishmeal substitution; soy protein concentrate; Paralichthys olivaceus; gene expression; growth performance; feed utilization

Online: 9 September 2024 (12:08:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0684.v1

The Shift from Aerobic to Anaerobic/Strength Training: A Paradigm Change in Preventing Frailty, Morbidity, and Mortality in Older Adults

Richard Murdoch Montgomery

Subject: Biology And Life Sciences, Aging Keywords: frailty syndrome; aerobic exercise; anaerobic exercise; strength training; older adults; morbidity; mortality

Online: 9 September 2024 (11:56:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0683.v1

Comparative Evaluation of the Chemical Components and Anti-inflammatory Potential of Yellow- and Blue-Flowered Meconopsis Species: M. integrifolia and M. betonicifoliaCitation: To Be Added by Editorial Staff during Production

Peizhao Cheng, Ruixi Gan, Cong Wang, Qian Xu, Kelsang Norbu, Feng Zhou, Sixin Kong, Zhuoma Jia, Dawa Jiabu, Xin Feng, Junsong Wang

Subject: Biology And Life Sciences, Plant Sciences Keywords: Meconopsis; Meconopsis integrifolia; Meconopsis betonicifolia; Anti-inflammation; Metabolomics; Traditional Tibetan medicine; Chemical composition

Online: 9 September 2024 (11:51:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0655.v1

Dose-Dependent Effects of Turmeric (Curcuma aromatica S.) Starch on Colonic Fermentation in Rats

E.M.A.C. Ekanayake, Ryota Ishii, Ryuji Nagata, Ken-ichiro Shimada, Kyu-Ho Han, Michihiro Fukushima

Subject: Biology And Life Sciences, Food Science And Technology Keywords: acetate; gut microbiota; turmeric starch; visceral fat

Online: 9 September 2024 (10:03:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0635.v1

Selenomethionine Mitigates Effects of Nocardia cyriacigeorgica-Induced Inflammation, Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells

Talgat Assabayev, Jinge Han, Halihaxi Bahetijiang, Venera Abdrassilova, Muhammad Asfandyar Khan, Herman W. Barkema, Gang Liu, John P. Kastelic, Xueying Zhou, Bo Han

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: bovine mastitis; Nocardia cyriacigeorgica; selenomethionine; oxidative stress; apoptosis

Online: 9 September 2024 (09:21:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0618.v1

Functional Analysis of Genetic Tests in Neurological Diagnostics: Advances and Future Prospects

Richard Murdoch Montgomery

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Neurological diagnostics; Genetic technologies; Microarray analysis Variant sequencing; Exome sequencing; RNA sequencing; Whole genome sequencing; Functional analysis; Personalized medicine; Neurological disorders

Online: 9 September 2024 (09:21:13 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202408.0190.v3

Cell Biology of Knee Joint Injuries: Early Mechanical Loading Perspective

Bartłomiej Kacprzak, Mikołaj Stańczak

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: knee joint; cell biology; mechanical loading

Online: 9 September 2024 (09:21:05 CEST)

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Knee joint injuries, including those affecting the anterior cruciate ligament (ACL), meniscus, and cartilage, present complex challenges in sports medicine and orthopedics due to the intricate cellular and molecular mechanisms involved in tissue damage and repair. Understanding the molecular biology underpinning these processes is crucial for developing effective therapeutic and rehabilitation strategies. This systematic review investigates the impact of mechanical loading on the cellular responses during knee joint injury repair, with a particular focus on the molecular pathways involved in tissue regeneration. Mechanical loading plays a dual role, where controlled early loading can promote tissue repair, while excessive or inappropriate loading can exacerbate tissue damage. Fibroblasts, chondrocytes, and mesenchymal stem cells (MSCs) are central to the repair process, and their activation, proliferation, and differentiation are regulated by key molecular pathways. Upon injury, mechanotransduction pathways such as the integrin/FAK signaling axis are activated, which convert mechanical signals into biochemical responses that regulate cell adhesion, migration, and extracellular matrix (ECM) synthesis. Additionally, mechanosensitive ion channels like Piezo1 and TRPV4 modulate intracellular calcium levels, triggering downstream signaling cascades such as calmodulin/CaMKII, which regulate gene transcription and cellular responses to mechanical stress. The YAP/TAZ pathway, a critical component of the Hippo signaling pathway, responds to mechanical stimuli and regulates cell proliferation and ECM production in fibroblasts and chondrocytes. YAP/TAZ translocate to the nucleus in response to mechanical loading, where they interact with transcription factors such as TEAD, promoting the expression of genes involved in collagen synthesis and tissue repair. In parallel, growth factors like transforming growth factor-beta (TGF-β) and fibroblast growth factor (FGF) activate the TGF-β/Smad and PI3K/Akt signaling pathways, driving MSC differentiation into fibroblasts and chondrocytes, essential for ligament and cartilage repair. Mechanical loading also influences the inflammatory response at the injury site by modulating immune cell activity. Early mechanical loading can shift macrophages from a pro-inflammatory M1 phenotype to an anti-inflammatory M2 phenotype, mediated by growth factors such as TGF-β and interleukin-10 (IL-10). This phenotypic switch promotes tissue regeneration by enhancing ECM synthesis and resolving inflammation, which is crucial for long-term joint health. Matrix metalloproteinases (MMPs), regulated by NF-κB and MAPK pathways, play a role in ECM remodeling, where an imbalance between MMPs and their inhibitors (TIMPs) can lead to excessive matrix degradation, impeding tissue repair. Furthermore, Wnt/β-catenin signaling is activated in response to moderate mechanical loading, promoting chondrocyte proliferation and enhancing cartilage repair by upregulating type II collagen and aggrecan synthesis. However, dysregulation of Wnt signaling under excessive mechanical stress can lead to chondrocyte hypertrophy and cartilage degradation, contributing to osteoarthritis development. This review also explores emerging therapeutic strategies that leverage the molecular biology of knee joint repair, including biologics like platelet-rich plasma (PRP) and MSC-derived exosomes, which deliver bioactive molecules that activate critical regenerative pathways such as TGF-β/Smad and PI3K/Akt. Gene therapies targeting Wnt signaling or YAP/TAZ offer potential for enhancing tissue regeneration by modulating mechanotransduction and repair processes at the molecular level. In conclusion, the molecular biology of cellular responses to mechanical loading is central to knee joint repair following injuries. By understanding these processes and targeting specific molecular pathways, clinicians can optimize rehabilitation protocols and develop novel therapeutic approaches that enhance tissue regeneration, prevent chronic degeneration, and restore joint function. This comprehensive synthesis highlights the importance of integrating molecular insights into treatment strategies for ACL, meniscal, and cartilage injuries to improve patient outcomes.

Preprint REVIEW | doi:10.20944/preprints202409.0642.v1

Conformational Mobility of Voltage-Gated Ion Channels

Ekaterina Kravchuk, Elizaveta Trifonova, Olga S. Sokolova

Subject: Biology And Life Sciences, Biophysics Keywords: voltage-gated ion channels; conformation; membrane mimetics; cryoelectron microscopy; in situ analysis; coordinated motion

Online: 9 September 2024 (09:20:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0584.v1

Sex/Gender Differences in Migraine: Exploring Pathophysiology, the Impact of Sex Hormones, and Tailored Therapeutic Approaches

Faizan Ahmad, Dr Rabeet Tariq, Safa Kaleem

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Migraine; sex hormones; pathology; treatment

Online: 9 September 2024 (09:16:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0585.v1

A Novel Approach to Compute Discrete Nonlinear Single Unit Activity Features

Nikita Zakharov, Elena Belova, Anna Gamaleya, Alexey Tomskiy, Alexey Sedov

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Entropy; mutual information; single unit activity; basal ganglia; subthalamic nucleus; Parkinson’ s disease

Online: 9 September 2024 (09:04:35 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0587.v1

Innovative Strategies and Methodologies in Antimicrobial Peptide Design

Devesh Pratap Verma, Amit Tripathi, Ashwani Kumar Kumar Thakur

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Antimicrobial Peptide Design; AI/ML; Antimicrobial Resistance

Online: 9 September 2024 (08:42:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0617.v1

Colchicine-Induced Double Haploid Production of Spring Bread Wheat (Triticum aestivum L.) via Wheat × Maize Hybridization

Md. Forhad Amin, Mohammad Nur Alam, Golam Faruq, Md. Mustafa Khan, Md. Mahbubur Rahman

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Colchicine; Conventional breeding method; Double haploid; Spring bread wheat; wheat ×; maize hybridization

Online: 9 September 2024 (08:42:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0615.v1

Brassica rapa BrICE1 and BrICE2 Positively Regulate the Cold Tolerance via CBFs and ROS Pathways, Balancing Growth and Defense in Transgenic Arabidopsis

Wangze Wu, Haobo Yang, Peng Xing, Guoting Zhu, Xueyan Han, Mei Xue, Guotai Min, Haijun Ding, Guofan Wu, Zigang Liu

Subject: Biology And Life Sciences, Plant Sciences Keywords: BrICE1 and BrICE2; freezing tolerance; phylogenetic tree; CBF pathway; Brassica rapa

Online: 9 September 2024 (08:42:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0626.v1

Identification of Some Potential Host Cellular Receptors and Enzymes that Potentially Refine the Bovine Coronavirus (BCoV) Tissue Tropism, Replication, and Pathogenesis

Mohd Yasir Khan, Abid Ullah Shah, Nithyadevi Duraisamy, Reda Nacif ElAlaoui, Mohammed Cherkaoui, Maged Gomaa Hemida

Subject: Biology And Life Sciences, Virology Keywords: BCoV; spike glycoprotein; Hemagglutinin esterase; ACE-2; NPR-1; Furin; TMPRRS2; docking; Neu5,9Ac2; Homology modeling; virus/host interaction; NGS; expression profile; q-RT-PCR ;

Online: 9 September 2024 (07:34:26 CEST)

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The information about the BCoV receptors, co/receptors that might be involved in the BCoV entry to the host cells is inconceivable. In addition to that, the roles of other host cell proteases, such as Furin and TMPRSS2, have not yet been well studied during BCoV replication. The current study’s main objectives are to identify some novel BCoV receptors and host cell proteases that might fine-tune the BCoV replication, tissue tropism, and molecular pathogenesis. We used a combination of some in silico prediction, molecular docking, and gene expression profile tools to predict some new BCoV receptors out of some known other coronavirus receptors and some host cell enzymes that are potentially involved in BCoV replication. To achieve these goals, we applied the in-silico prediction tools to check the potential interactions between eh BCoV/S glycoprotein and some known coronaviruses receptors (ACE2, APN1, NPR1, CECAM-1, AXL, and DPP4). We also established some potential models for the interaction between the BCoV/S and some common host cell proteases proved to play important roles in other coronavirus replication cycle (Furin, TMPRRS2, and Cathepsin-L). To confirm our prediction models, we assessed the gene expression profiles of these potential receptors and proteases in some bovine cell lines infected with BCoV using the next generation sequencing and the qRT-PCR assay. Our results show that the NPR1, AXL1 and the ACE2 have the strongest and firm binding affinities to the BCoV-S protein. Our prediction models and gene expression profiles show the potential bindings of the Furin and TMPRSS2 to the BCoV-S polybasic cleavage site (RRSRR|A). Notably, high expression of the host cell Furin was observed in response to both BCoV/Ent and BCoV/Resp infection, whereas TMPRSS2 expression was upregulated in the case of the BCoV/Ent infection, positioning Furin as a common receptor for different BCoV isolates. Further research is needed to study the roles of these top-ranked receptors and host cell proteases in molecular pathogenesis, and tissue tropism.

Biology and Life Sciences