Extended Data Fig. 4: Stroke differentially impacts lymphocyte numbers in lymphoid tissues and is independent of reduced food consumption.

a, Total numbers of CD19⁺ B cells and CD3⁺ T cells in spleens of stroke or sham−operated mice (n = 12−14 per group, two−tailed Mann−Whitney U test, ****P < 0.0001, *P = 0.0202). b-d, Total numbers of CD19⁺ B cells and CD3⁺ T cells in mLN (n = 12−14 per group, two−tailed Mann−Whitney U test, CD19⁺ cells P = 0.3744, CD3+ cells P = 0.5952), BM (n = 12−14 per group, two−tailed Mann−Whitney U test, CD19⁺ P = 0.6308, CD3⁺ P = 0.3217) and blood (n = 12−14 per group, two−tailed Mann−Whitney U test, CD19⁺ P = 0.5267, CD3⁺ P = 0.7045) of stroke or sham−operated mice. e, The percentage change in IgG⁺ and IgM⁺ B cells in PP after 24 h of sham or stroke operation (n = 4 mice per group, unpaired t-test, ***P = 0.0005, **P = 0.0064). Data is presented as normalized to the mean of sham. f, Relative frequencies of B cells in blood, mLN and SI LP after 12 h of stroke or sham−operation (n = 3−4 per group) normalized to mean of sham. g, Mice underwent stroke or sham surgery and received two times food gavage and were sacrificed 24 h after the operation to analyze the number of B and T cells in PP. h, Total numbers of CD19⁺ B cells and CD3⁺ T cells in PP of stroke or sham−operated mice that received food gavage (n = 7 per group, two−tailed Mann−Whitney U test, ***P = 0.0006, **P = 0.0023). i, Percentage of body-weight loss in sham and stroke mice with and without food gavage (n = 6−8 per group, two−tailed Mann−Whitney U test, no gavage sham vs stroke P = 0.2284, food gavage sham vs stroke P = 0.5589). j, Quantification of plasma DNA content in sham−operated or MI mice (n = 5−8 per group, ordinary one−way ANOVA, ****P < 0.0001). k, Cell cultures from PP were prepared and treated with the plasma of sham or stroke mice for 12 h. In the third condition, plasma of stroke mice was treated with DNase−I before addition to the cell cultures and quantification of Annexin V⁺ PI⁻ cells was performed by flow cytometry (n = 5 per group). Data represented as mean ±s.d., Shapiro-Wilk normality test. PP=Peyer’s patches, SI LP=small intestine lamina propria, mLN=mesenteric lymph nodes, MI=myocardial infarction.