Extended Data Fig. 2: Mass spectrometry analysis of SthK.
From: Anionic lipids unlock the gates of select ion channels in the pacemaker family
(a) Lipidomics analysis of the phospholipids extracted from the SthK sample (top) and E. coli phospholipids (bottom). PE and PG species are annotated with the length of the acyl chains and the number of double bonds. PG is enriched in the SthK sample. (b) Identification of cardiolipins (CL) in the SthK sample. CL species are annotated with the acyl chain length and the number of double bonds (left panel). The right panel shows the MS/MS identification of CL (68:3). (c) Probability of 0, 1, 2, 3, or 4 cAMP-binding events to SthK as observed in native MS are plotted in grey, red, olive, green and blue, respectively.
