Extended Data Fig. 5: Gα13 signaling promotes Cyclin D3 degradation.
From: Gα13 restricts nutrient driven proliferation in mucosal germinal centers
(a, b) Relative frequency of GNA13 sgRNA-expressing OCI-LY8 (a) or Dogkit (b) cells with or without U46619. One experiment representative of 2. (c) Experimental scheme for data in 4 l. (d) Quantitative PCR of CCND3 in NUDUL1 cells treated with U46619. Each point represents a technical replicate. One experiment representative of 2. (e-f) Cyclin D3 protein expression in NUDUL1 cells treated with U46619 and MLN4924 for 3 hours (e) or 6 hours (f). One experiment representative of three or two, respectively. (g) Phospho-Cyclin D3 expression in NUDUL1 cells treated with U46619 and MLN4924 for 1 hour. One experiment representative of 2.
