Directed evolution is commonly performed in prokaryotic or yeast systems, but platforms are needed to enhance functions in mammalian cells. Now, a tool known as RNA replicase-assisted continuous evolution (REPLACE) enables directed evolution in mammalian cells via mutagenesis and amplification of RNA and selection for desired phenotypes.
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The authors are both inventors on filed Australian provisional patent 2023904160, which describes a viral directed evolution platform.
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Denes, C.E., Neely, G.G. REPLACE-ing RNA through evolution. Nat Chem Biol 21, 322–323 (2025). https://doi.org/10.1038/s41589-025-01845-z
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DOI: https://doi.org/10.1038/s41589-025-01845-z
