Supplementary Figure 1: CCS protocol development. | Nature Biotechnology

Supplementary Figure 1: CCS protocol development.

From: Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome

Supplementary Figure 1

(a) Distribution of polymerase read lengths for a 10 kb E.coli amplicon library and 30 kb E. coli whole genome library sequenced for 10 hours with identical conditions. (b) Distribution of polymerase read lengths for an 8 kb fragment from a BsaAI-digested lambda library sequenced for 4 hours with (5 hour) and without (0 hour) pre-extension to reduce “early-terminating” reads and select surviving polymerase-template complexes. (c) Sample preparation and sequencing workflow. (d) BioAnalyzer trace for the SMRTbell library, sheared to target 15–20 kb fragments. “FU” is fluorescence units. (e) BioAnalyzer trace for ELF fractions of the SMRTbell library. (f) The fraction centered around 15 kb was used for sequencing.

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