Extended Data Fig. 10: Analysis the effects of GSK2643943A on Ucp1 KO or L-Usp20−/− mice.
From: Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis
a–l, Eight-week-old male Ucp1 KO mice and their male WT littermates were randomly grouped, fed the HFHS diet and gavaged with vehicle or 30 mg kg-1 GSK2643943A daily for 13 days. GTT and metabolic cage analysis were performed on day 7 and 10, respectively. a, Immunoblotting analysis. *: non-specific band. b, Body weight per mouse (n = 3 per group) during the experiments. c, Cumulative food intake for each treatment. Each value represents mean of data from three mice. d, e, Whole-body oxygen consumption of the mice (n = 3 per group) measured by metabolic cages. f, Respiratory exchange ratio (RER) over dark and light cycles (n = 3 per group). g, h, GTT analysis (n = 3 per group). i–l, TC (i), TG (j), AST (k), and ALT (l) in the serum (n = 3 per group). m–s, Eight-week-old male L-Usp20−/− mice and their male WT littermates were randomly grouped, fed the HFHS diet and gavaged with vehicle or 30 mg kg-1 GSK2643943A daily for 13 days. GTT and metabolic cage analysis were performed on day 7 and 10, respectively. m, Immunoblotting analysis. n, o, Whole-body oxygen consumption of the mice (n = 4 per group) measured by metabolic cages. p, q, GTT analysis (n = 4 per group). r, Total cholesterol in serum (n = 4 per group). s, Triglyceride in serum (n = 4 per group). All values are expressed as means ± SEM. Experiments in (a, m) were performed as indicated twice with similar results. Data were analysed by unpaired two-tailed Student’s t-test (e, f, h–l, o, q–s), or two-way ANOVA (b, d, g, n, p).
