Supplementary Figure 7: Further validation of lncRNA transcripts.
From: The landscape of long noncoding RNAs in the human transcriptome
(a) Heat-map representation of the correlation between qPCR (fold change over the median) with RNA-seq (FPKM) of 100 selected transcripts in the A549, LNCaP and MCF-7 cell lines. (b,c) Representative example of 2 of 20 previously unannotated lncRNA transcripts that were analyzed by Sanger sequencing to ensure primer specificity with their associated chromatograms. As seen in the UCSC Genome Browser View, a (b) multiexonic lncRNA (Gene ID: G021137) and (c) monoexonic lncRNA (Gene ID: G030545).
