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Search Results (4,998)

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18 pages, 5471 KiB  
Article
Evaluation of Short-Season Soybean (Glycine max (L.) Merr.) Breeding Lines for Tofu Production
by Mehri Hadinezhad, Simon Lackey and Elroy R. Cober
Seeds 2024, 3(3), 393-410; https://doi.org/10.3390/seeds3030028 - 14 Aug 2024
Abstract
Soybean breeding programs targeting tofu quality must evaluate their performance within zones of adaptation. A comprehensive study was carried out to examine soybean breeding lines from three maturity groups (MGs; MG0, MG00, and MG000) from 2018 to 2022. Several agronomic, chemical composition and [...] Read more.
Soybean breeding programs targeting tofu quality must evaluate their performance within zones of adaptation. A comprehensive study was carried out to examine soybean breeding lines from three maturity groups (MGs; MG0, MG00, and MG000) from 2018 to 2022. Several agronomic, chemical composition and tofu-related quality traits were evaluated, and the associations among traits were investigated. The results showed that genotypes in MG0 yielded higher and matured later, which confirmed that the selection of targeted genotypes for a specific maturity group was successful. Non-imbibed “stone seeds”, an important quality trait for tofu processors, were higher in MG000 lines. Tofu texture using both GDL and MgCl2 coagulants was positively associated, indicating one coagulant might be enough for screening purposes. The MG by traits biplot showed very clear MG clustering for all genotypes tested from 2018 to 2022, signifying that the MG has a more pronounced effect on the investigated traits than the environmental effects seen in different years, regardless of the MG. Most tofu-related traits were higher and showed stronger associations in MG0 lines compared to the lines in earlier MGs, indicating a need for future effort in shorter season MGs. Overall, this study provided useful information for selecting soybean lines for tofu end-use application targeting specific MGs. Full article
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Figure 1
<p>Mean values for temperature (columns) and total precipitation (blue lines) from May to October between 2018 and 2022. Error bars on columns are ±SE.</p>
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<p>Mean values of yield (<b>a</b>), maturity (<b>b</b>), plant height (<b>c</b>), and lodging (<b>d</b>) of all tested genotypes in each year for a given MG. Error bars are ±SE.</p>
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<p>Mean values of tofu-related traits, including stones (%), Brix (%), dry matter (% of soy milk), and tofu texture firmness (N force), using GDL or MgCl<sub>2</sub> coagulants. Error bars are ±SE.</p>
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<p>GGEBiplot visualization of trait association for all genotypes tested in 2018. Traits comprised yield, maturity, plant height, lodging, seed quality, stone seeds (STONES), seed weight, protein, oil, carbohydrates (CARBO), sugar, sucrose, raffinose and stachyose (RAFFSTACH), soymilk Brix, soymilk dry matter, and tofu texture firmness using GDL and MgCl<sub>2</sub> (TEXTURE GDL and TEXTURE MGCL2, respectively). The circles represent genotypes in MG0 (blue circles) and MG00 (green circles). The genotype names are presented in <a href="#app1-seeds-03-00028" class="html-app">Table S2</a>.</p>
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<p>GGEBiplot visualization of trait association for all genotypes tested in 2019. Traits comprised yield, maturity, plant height, lodging, seed quality, stone seeds (STONES), seed weight, protein, oil, carbohydrates (CARBO), sugar, sucrose, raffinose and stachyose (RAFFSTACH), soymilk Brix, soymilk dry matter, and tofu texture firmness using GDL and MgCl<sub>2</sub> (TEXTURE GDL and TEXTURE MGCL2, respectively). The circles represent genotypes in MG0 (blue circles), MG00 (green circles), and MG000 (brown circles). The genotype names are presented in <a href="#app1-seeds-03-00028" class="html-app">Table S2</a>.</p>
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<p>GGEBiplot visualization of trait association for all genotypes tested in 2020. Traits comprised yield, maturity, plant height, lodging, seed quality, stone seeds (STONES), seed weight, protein, oil, carbohydrates (CARBO), sugar, sucrose, raffinose and stachyose (RAFFSTACH), soymilk Brix, soymilk dry matter, and tofu texture firmness using GDL and MgCl<sub>2</sub> (TEXTURE GDL and TEXTURE MGCL2, respectively). The circles represent genotypes in MG0 (blue circles) and MG00 (green circles). The genotype names are presented in <a href="#app1-seeds-03-00028" class="html-app">Table S2</a>.</p>
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<p>GGEBiplot visualization of trait association for all genotypes tested in 2021. Traits comprised yield, maturity, plant height, lodging, seed quality, stone seeds (STONES), seed weight, protein, oil, carbohydrates (CARBO), sugar, sucrose, raffinose and stachyose (RAFFSTACH), soymilk Brix, soymilk dry matter, and tofu texture firmness using GDL and MgCl<sub>2</sub> (TEXTURE GDL and TEXTURE MGCL2, respectively). The circles represent genotypes in MG0 (blue circles), MG00 (green circles), and MG000 (brown circles). The genotype names are presented in <a href="#app1-seeds-03-00028" class="html-app">Table S2</a>.</p>
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<p>GGEBiplot visualization of trait association for all genotypes tested in 2022. Traits comprised yield, maturity, plant height, lodging, seed quality, stone seeds (STONES), seed weight, protein, oil, carbohydrates (CARBO), sugar, sucrose, raffinose and stachyose (RAFFSTACH), soymilk Brix, soymilk dry matter, and tofu texture firmness using GDL and MgCl<sub>2</sub> (TEXTURE GDL and TEXTURE MGCL2, respectively). The circles represent genotypes in MG0 (blue circles) and MG00 (green circles). The genotype names are presented in <a href="#app1-seeds-03-00028" class="html-app">Table S2</a>.</p>
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<p>GGEBiplot visualization of trait by MG in all years and for all the genotypes tested. Traits comprised yield, maturity, plant height, lodging, seed quality, stone seeds (STONES), seed weight, protein, oil, carbohydrates (CARBO), sugar, sucrose, raffinose and stachyose (RAFFSTACH), soymilk Brix, soymilk dry matter, and tofu texture firmness using GDL and MgCl<sub>2</sub> (TEXTURE GDL and TEXTURE MGCL2, respectively). Mg0, Mg00, and Mg000 refer to the different maturity groups.</p>
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19 pages, 2894 KiB  
Article
Impact of Crop Residue, Nutrients, and Soil Moisture on Methane Emissions from Soil under Long-Term Conservation Tillage
by Rajesh Choudhary, Sangeeta Lenka, Dinesh Kumar Yadav, Narendra Kumar Lenka, Rameshwar S. Kanwar, Abhijit Sarkar, Madhumonti Saha, Dharmendra Singh and Tapan Adhikari
Soil Syst. 2024, 8(3), 88; https://doi.org/10.3390/soilsystems8030088 - 13 Aug 2024
Viewed by 203
Abstract
Greenhouse gas emissions from agricultural production systems are a major area of concern in mitigating climate change. Therefore, a study was conducted to investigate the effects of crop residue, nutrient management, and soil moisture on methane (CH4) emissions from maize, rice, [...] Read more.
Greenhouse gas emissions from agricultural production systems are a major area of concern in mitigating climate change. Therefore, a study was conducted to investigate the effects of crop residue, nutrient management, and soil moisture on methane (CH4) emissions from maize, rice, soybean, and wheat production systems. In this study, incubation experiments were conducted with four residue types (maize, rice, soybean, wheat), seven nutrient management treatments {N0P0K0 (no nutrients), N0PK, N100PK, N150PK, N100PK + manure@ 5 Mg ha−1, N100PK + biochar@ 5 Mg ha−1, N150PK+ biochar@ 5 Mg ha−1}, and two soil moisture levels (80% FC, and 60% FC). The results of this study indicated that interactive effects of residue type, nutrient management, and soil moisture significantly affected methane (CH4) fluxes. After 87 days of incubation, the treatment receiving rice residue with N100PK at 60% FC had the highest cumulative CH4 mitigation of −19.4 µg C kg−1 soil, and the highest emission of CH4 was observed in wheat residue application with N0PK at 80% FC (+12.93 µg C kg−1 soil). Nutrient management had mixed effects on CH4 emissions across residue and soil moisture levels in the following order: N150PK > N0PK > N150PK + biochar > N0P0K0 > N100PK + manure > N100PK + biochar > N100PK. Decreasing soil moisture from 80% FC to 60% FC reduced methane emissions across all residue types and nutrient treatments. Wheat and maize residues exhibited the highest carbon mineralization rates, followed by rice and soybean residues. Nutrient inputs generally decreased residue carbon mineralization. The regression analysis indicated that soil moisture and residue C mineralization were the two dominant predictor variables that estimated 31% of soil methane fluxes in Vertisols. The results of this study show the complexity of methane dynamics and emphasize the importance of integrated crop, nutrient, and soil moisture (irrigation) management strategies that need to be developed to minimize methane emissions from agricultural production systems to mitigate climate change. Full article
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Graphical abstract

Graphical abstract
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<p>Cumulative soil methane (CH<sub>4</sub>) flux (µg-C kg<sup>−1</sup> soil) (<b>a</b>) effect of residue types and soil moisture across nutrient management, (<b>b</b>) effect of nutrient management and soil moisture across residue treatment, and (<b>c</b>) effect of nutrient management and residue types across soil moisture treatment. Vertical bars represent mean ± standard error (n = 3). Different lower-case letters indicate significant differences among treatments at α &lt; 0.05.</p>
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<p>Cumulative soil methane (CH<sub>4</sub>) flux (µg-C kg<sup>−1</sup> soil) (<b>a</b>) effect of residue types and soil moisture across nutrient management, (<b>b</b>) effect of nutrient management and soil moisture across residue treatment, and (<b>c</b>) effect of nutrient management and residue types across soil moisture treatment. Vertical bars represent mean ± standard error (n = 3). Different lower-case letters indicate significant differences among treatments at α &lt; 0.05.</p>
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<p>The apparent residue C mineralization (% residue C yr<sup>−1</sup>) (<b>a</b>) effect of residue types and soil moisture across nutrient management, (<b>b</b>) effect of nutrient management and soil moisture across residue treatment, and the effect of nutrient management and residue types across soil moisture treatment were found to be nonsignificant; therefore, the figure is given in the <a href="#app1-soilsystems-08-00088" class="html-app">Supplementary File as Figure S3</a>. Vertical bars represent the mean ± standard error (n = 3). Different lower-case letters indicate significant differences among treatments at α &lt; 0.05.</p>
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16 pages, 2466 KiB  
Article
Comparative Analysis of the Mitochondrial Genome Sequences of Diaporthe longicolla (syn. Phomopsis longicolla) Isolates Causing Phomopsis Seed Decay in Soybean
by Shuxian Li, Xiaojun Hu and Qijian Song
J. Fungi 2024, 10(8), 570; https://doi.org/10.3390/jof10080570 - 13 Aug 2024
Viewed by 250
Abstract
Diaporthe longicolla (syn. Phomopsis longicolla) is an important seed-borne fungal pathogen and the primary cause of Phomopsis seed decay (PSD) in soybean. PSD is one of the most devastating seed diseases, reducing soybean seed quality and yield worldwide. As part of a genome [...] Read more.
Diaporthe longicolla (syn. Phomopsis longicolla) is an important seed-borne fungal pathogen and the primary cause of Phomopsis seed decay (PSD) in soybean. PSD is one of the most devastating seed diseases, reducing soybean seed quality and yield worldwide. As part of a genome sequencing project on the fungal Diaporthe–Phomopsis complex, draft genomes of eight D. longicolla isolates were sequenced and assembled. Sequences of mitochondrial genomes were extracted and analyzed. The circular mitochondrial genomes ranged from 52,534 bp to 58,280 bp long, with a mean GC content of 34%. A total of 14 core protein-coding genes, 23 tRNA, and 2 rRNA genes were identified. Introns were detected in the genes of atp6, cob, cox1, cox2, cox3, nad1, nad2, nad5, and rnl. Three isolates (PL7, PL10, and PL185E) had more introns than other isolates. Approximately 6.4% of the mitochondrial genomes consist of repetitive elements. Moreover, 48 single-nucleotide polymorphisms (SNPs) and were identified. The mitochondrial genome sequences of D. longicolla will be useful to further study the molecular basis of seed-borne pathogens causing seed diseases, investigate genetic variation among isolates, and develop improved control strategies for Phomopsis seed decay of soybean. Full article
(This article belongs to the Section Fungal Genomics, Genetics and Molecular Biology)
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<p>Circular maps of the mitochondrial genomes of eight <span class="html-italic">Diaporthe longicolla</span> isolates.</p>
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<p>Codon usage in the mitochondrial genome of <span class="html-italic">Diaporthe longicolla</span> isolate PL185E. Codon families are plotted on the X axis and represented by different color patches. Frequency of codon usage is plotted on the Y axis. The codon usage was calculated by the Sequence Manipulation Suite (<a href="https://www.bioinformatics.org/sms2/codon_usage.html" target="_blank">https://www.bioinformatics.org/sms2/codon_usage.html</a>, accessed on 1 November 2023) with genetic code 4.</p>
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<p>Putative secondary structures of the 23 tRNA genes from PL mitochondrial genome. The tRNAs are labeled with the abbreviations od their corresponding ammino acids. The tRNA arms are illustrated as from <span class="html-italic">trnT</span>. The map of tRNA structures was drawn using the MITOS web server (<a href="http://mitos.bioinf.uni-leipzig.de/index.py" target="_blank">http://mitos.bioinf.uni-leipzig.de/index.py</a>, accessed on 1 November 2023).</p>
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<p>Dispersed and inverted repeat sequences in <span class="html-italic">Diaporthe longicolla</span> mitochondrial genome. Colors: black, conserved protein-coding, rRNA and tRNA genes; grey, introns; white, intergenic regions. Red lines connect regions of significant (E-value &lt; 1 × 10<sup>−10</sup>) nucleotide sequence similarity.</p>
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26 pages, 1850 KiB  
Article
Fuzzy Logic Theory and Spatiotemporal Modeling of the Fungus Phakopsora pachyrhizi Based on Differential Equations
by Nayara Longo Sartor Zagui, Andre Krindges, Carlos Roberto Minussi and Moiseis dos Santos Cecconello
Appl. Sci. 2024, 14(16), 7082; https://doi.org/10.3390/app14167082 - 12 Aug 2024
Viewed by 258
Abstract
Brazil has been one of the largest soybean producers in recent years. The soybean is a legume commonly found in family meals. Among the diseases affecting the grains, Asian soybean rust is one of the most concerning. The fungus causing the disease is [...] Read more.
Brazil has been one of the largest soybean producers in recent years. The soybean is a legume commonly found in family meals. Among the diseases affecting the grains, Asian soybean rust is one of the most concerning. The fungus causing the disease is spread by the wind, making it difficult to control. Although it has been researched since its first records, not much data are available regarding the macro propagation behavior of spores. Therefore, this research aimed to model its dispersion based on a partial differential equation, the diffusion–advection equation, used by researchers to model the behavior of any pollutant. The terms of this equation were developed from real data, processed by fuzzy logic, and the simulation results were compared with disease records throughout a harvest. By using this approach to model the spatiotemporal dynamics of this fungus, it was possible to simulate its spread satisfactorily. Additionally, its results were used as input variables for a fuzzy system that estimates the susceptibility of a given location to disease development. Full article
(This article belongs to the Special Issue Fuzzy Control Systems: Latest Advances and Prospects)
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<p>Von Neumann-type boundary conditions.</p>
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<p>Clipping vector field.</p>
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<p>Clipping vector field.</p>
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<p>Fuzzy Decay System.</p>
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<p>Fuzzy Set Decay System.</p>
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<p>Fuzzy Set Decay System.</p>
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<p>Fuzzy Set Decay System.</p>
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<p>Fuzzy Set Decay System.</p>
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<p>Occurrences by harvest versus highways.</p>
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<p>Spore concentrations throughout the harvest.</p>
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<p>Spore concentrations for locations where occurrences were recorded.</p>
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19 pages, 7603 KiB  
Article
SPCN: An Innovative Soybean Pod Counting Network Based on HDC Strategy and Attention Mechanism
by Ximing Li, Yitao Zhuang, Jingye Li, Yue Zhang, Zhe Wang, Jiangsan Zhao, Dazhi Li and Yuefang Gao
Agriculture 2024, 14(8), 1347; https://doi.org/10.3390/agriculture14081347 - 12 Aug 2024
Viewed by 299
Abstract
Soybean pod count is a crucial aspect of soybean plant phenotyping, offering valuable reference information for breeding and planting management. Traditional manual counting methods are not only costly but also prone to errors. Existing detection-based soybean pod counting methods face challenges due to [...] Read more.
Soybean pod count is a crucial aspect of soybean plant phenotyping, offering valuable reference information for breeding and planting management. Traditional manual counting methods are not only costly but also prone to errors. Existing detection-based soybean pod counting methods face challenges due to the crowded and uneven distribution of soybean pods on the plants. To tackle this issue, we propose a Soybean Pod Counting Network (SPCN) for accurate soybean pod counting. SPCN is a density map-based architecture based on Hybrid Dilated Convolution (HDC) strategy and attention mechanism for feature extraction, using the Unbalanced Optimal Transport (UOT) loss function for supervising density map generation. Additionally, we introduce a new diverse dataset, BeanCount-1500, comprising of 24,684 images of 316 soybean varieties with various backgrounds and lighting conditions. Extensive experiments on BeanCount-1500 demonstrate the advantages of SPCN in soybean pod counting with an Mean Absolute Error(MAE) and an Mean Squared Error(MSE) of 4.37 and 6.45, respectively, significantly outperforming the current competing method by a substantial margin. Its excellent performance on the Renshou2021 dataset further confirms its outstanding generalization potential. Overall, the proposed method can provide technical support for intelligent breeding and planting management of soybean, promoting the digital and precise management of agriculture in general. Full article
(This article belongs to the Special Issue Computer Vision and Artificial Intelligence in Agriculture)
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<p>Sample images of BeanCount-1500.</p>
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<p>Characteristics of the BeanCount-1500. (<b>a</b>) Different illuminations and backgrounds. (<b>b</b>) Diverse levels of clarity. (<b>c</b>) Diverse number of plants in each picture. (<b>d</b>) Shattering.</p>
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<p>Demonstration of the characteristics of the labelling process, where the red dots represent the labeled points.</p>
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<p>Distribution of BeanCount-1500. (<b>a</b>) Scatter plot of Resolution and soybean pod count distribution. (<b>b</b>) Box plot of soybean pod number distribution. (<b>c</b>) Scatter plot of resolution distribution. Note: For (<b>a</b>), the <span class="html-italic">xyz</span>-axis distribution represents the image height, width, and number of annotations. The lighter the color, the more annotations. For (<b>c</b>), we use the K-means clustering algorithm to classify the resolutions of different images, represented by different colors.</p>
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<p>Structure of the SPCN.</p>
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<p>Illustration of the Front-End module.</p>
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<p>The structure of CBAM.</p>
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<p>Diagrammatic representation of the Back-End module. (<b>a</b>) Schematic of the receptive field. (<b>b</b>) Back-end module diagram. Note: For (<b>a</b>), the more cells a color covers, the larger its receptive field.</p>
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<p>Schematic diagram of the loss function.</p>
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<p>Decreasing training loss graph.</p>
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<p>Decreasing training and validation MAE and MSE graph. (<b>a</b>) Decreasing MAE. (<b>b</b>) Decreasing MSE. Note: The blue line shows the convergence of the metric during training, whereas the red line shows the convergence of the metric during validation.</p>
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<p>Visualization of model predictions. The first column shows the input image with the actual number of pods in the upper right corner. The second to fourth columns display the density maps predicted by different models with the predicted number of pods in the upper right corner. The fifth column shows the distribution of annotation points. Yellow circles highlight areas where the SPCN model performs better.</p>
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<p>Prediction results on the Renshou2021 dataset. The first and third columns represent the input images, with the red font above them indicating the actual number of pods. The second and fourth columns represent the density maps predicted by SPCN, with the red font above them indicating the predicted number of pods.</p>
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20 pages, 6791 KiB  
Article
Quercetin Supplementation Improves Intestinal Digestive and Absorptive Functions and Microbiota in Rats Fed Protein-Oxidized Soybean Meal: Transcriptomics and Microbiomics Insights
by Zhiyong Wang, Peng Wang, Yanmin Zhou and Su Zhuang
Animals 2024, 14(16), 2326; https://doi.org/10.3390/ani14162326 - 12 Aug 2024
Viewed by 229
Abstract
To clarify the nutritional mechanisms of quercetin mitigation in the digestive and absorptive functions in rats fed protein-oxidized soybean meal, 48 three-week-old male SD rats were randomly allocated into a 2 × 2 factorial design with two soybean meal types (fresh soybean meal [...] Read more.
To clarify the nutritional mechanisms of quercetin mitigation in the digestive and absorptive functions in rats fed protein-oxidized soybean meal, 48 three-week-old male SD rats were randomly allocated into a 2 × 2 factorial design with two soybean meal types (fresh soybean meal or protein-oxidized soybean meal) and two quercetin levels (0 or 400 mg/kg) for a 28-day feeding trial. The protein-oxidized soybean meal treatment decreased (p < 0.05) the relative weights of the pancreas, stomach, and cecum, duodenal villus height, pancreatic and jejunal lipase activities, apparent ileal digestibility of amino acids, and apparent total tract digestibility of dry matter, crude protein, and ether extract. The supplementation of quercetin in the protein-oxidized soybean meal diet reversed (p < 0.05) the decreases in the duodenal length, ileal villus height, lipase activity, apparent ileal digestibility of amino acids, and apparent total tract digestibility of dry matter, crude protein, and ether extract. Transcriptomics revealed that the “alanine transport” and “lipid digestion and absorption” pathways were downregulated by the protein-oxidized soybean meal compared with fresh soybean meal, while the “basic amino acid transmembrane transporter activity” and “lipid digestion and absorption” pathways were upregulated by the quercetin supplementation. Microbiomics revealed that the protein-oxidized soybean meal increased the protein-degrading and inflammation-triggering bacteria in the cecum, while the relative abundances of beneficial bacteria were elevated by the quercetin supplementation. Full article
(This article belongs to the Special Issue Plant Extracts as Feed Additives in Animal Nutrition and Health)
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Figure 1
<p>The intestinal morphology of rats. FS: fresh soybean meal; FS + Q: fresh soybean meal + quercetin; OS: protein-oxidized soybean meal; OS + Q: protein-oxidized soybean meal + quercetin. (<b>A</b>) Duodenum. (<b>B</b>) Jejunum. (<b>C</b>) Ileum.</p>
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<p>The intestinal morphology of rats. FS: fresh soybean meal; FS + Q: fresh soybean meal + quercetin; OS: protein-oxidized soybean meal; OS + Q: protein-oxidized soybean meal + quercetin. (<b>A</b>) Duodenum. (<b>B</b>) Jejunum. (<b>C</b>) Ileum.</p>
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<p>Transcriptome analysis of jejunum. FS: fresh soybean meal; FS + Q: fresh soybean meal + quercetin; OS: protein-oxidized soybean meal; OS + Q: protein-oxidized soybean meal + quercetin. <span class="html-italic">n</span> = 4 for each group. (<b>A</b>) Principal component analysis (PCA) of transcriptional profiling of the rat jejunum tissues among four groups. (<b>B</b>) Differential gene expression analysis. Red dots (up) represent significantly upregulated genes (<span class="html-italic">p</span> &lt; 0.05, FC ≥ 2); green dots (down) represent significantly downregulated genes (<span class="html-italic">p</span> &lt; 0.05, FC ≤ 0.5); and gray dots (no) represent insignificant DEGs. (<b>C</b>) GO pathway enrichment analysis of top 20 pathways. The pathways with the red box are related to the intestinal digestive and absorptive functions. (<b>D</b>) KEGG pathway enrichment analysis of top 20 pathways. The pathways with the red box are related to the intestinal digestive and absorptive functions. (<b>E</b>) Significant pathways of GSEA analysis results in FS vs. OS comparison groups (nominal <span class="html-italic">p</span> &lt; 0.05). (<b>F</b>) Significant pathways of GSEA analysis results in OS vs. OS + Q comparison groups (nominal <span class="html-italic">p</span> &lt; 0.05).</p>
Full article ">Figure 2 Cont.
<p>Transcriptome analysis of jejunum. FS: fresh soybean meal; FS + Q: fresh soybean meal + quercetin; OS: protein-oxidized soybean meal; OS + Q: protein-oxidized soybean meal + quercetin. <span class="html-italic">n</span> = 4 for each group. (<b>A</b>) Principal component analysis (PCA) of transcriptional profiling of the rat jejunum tissues among four groups. (<b>B</b>) Differential gene expression analysis. Red dots (up) represent significantly upregulated genes (<span class="html-italic">p</span> &lt; 0.05, FC ≥ 2); green dots (down) represent significantly downregulated genes (<span class="html-italic">p</span> &lt; 0.05, FC ≤ 0.5); and gray dots (no) represent insignificant DEGs. (<b>C</b>) GO pathway enrichment analysis of top 20 pathways. The pathways with the red box are related to the intestinal digestive and absorptive functions. (<b>D</b>) KEGG pathway enrichment analysis of top 20 pathways. The pathways with the red box are related to the intestinal digestive and absorptive functions. (<b>E</b>) Significant pathways of GSEA analysis results in FS vs. OS comparison groups (nominal <span class="html-italic">p</span> &lt; 0.05). (<b>F</b>) Significant pathways of GSEA analysis results in OS vs. OS + Q comparison groups (nominal <span class="html-italic">p</span> &lt; 0.05).</p>
Full article ">Figure 2 Cont.
<p>Transcriptome analysis of jejunum. FS: fresh soybean meal; FS + Q: fresh soybean meal + quercetin; OS: protein-oxidized soybean meal; OS + Q: protein-oxidized soybean meal + quercetin. <span class="html-italic">n</span> = 4 for each group. (<b>A</b>) Principal component analysis (PCA) of transcriptional profiling of the rat jejunum tissues among four groups. (<b>B</b>) Differential gene expression analysis. Red dots (up) represent significantly upregulated genes (<span class="html-italic">p</span> &lt; 0.05, FC ≥ 2); green dots (down) represent significantly downregulated genes (<span class="html-italic">p</span> &lt; 0.05, FC ≤ 0.5); and gray dots (no) represent insignificant DEGs. (<b>C</b>) GO pathway enrichment analysis of top 20 pathways. The pathways with the red box are related to the intestinal digestive and absorptive functions. (<b>D</b>) KEGG pathway enrichment analysis of top 20 pathways. The pathways with the red box are related to the intestinal digestive and absorptive functions. (<b>E</b>) Significant pathways of GSEA analysis results in FS vs. OS comparison groups (nominal <span class="html-italic">p</span> &lt; 0.05). (<b>F</b>) Significant pathways of GSEA analysis results in OS vs. OS + Q comparison groups (nominal <span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Microbiomics analysis of cecal digesta. FS: fresh soybean meal; FS + Q: fresh soybean meal + quercetin; OS: protein-oxidized soybean meal; OS + Q: protein-oxidized soybean meal + quercetin. <span class="html-italic">n</span> = 5 for each group. (<b>A</b>) Changes in the α-diversity of cecal microbiota communities, as indicated by the chao1, goods_coverage, simpson, pielou_e, faith_pd, shannon, and observed_species indices. (<b>B</b>) PCoA of cecal microbiota. (<b>C</b>) The relative abundances of cecal microbiota at the phylum, family, genus, and species levels. (<b>D</b>) LDA scores.</p>
Full article ">Figure 3 Cont.
<p>Microbiomics analysis of cecal digesta. FS: fresh soybean meal; FS + Q: fresh soybean meal + quercetin; OS: protein-oxidized soybean meal; OS + Q: protein-oxidized soybean meal + quercetin. <span class="html-italic">n</span> = 5 for each group. (<b>A</b>) Changes in the α-diversity of cecal microbiota communities, as indicated by the chao1, goods_coverage, simpson, pielou_e, faith_pd, shannon, and observed_species indices. (<b>B</b>) PCoA of cecal microbiota. (<b>C</b>) The relative abundances of cecal microbiota at the phylum, family, genus, and species levels. (<b>D</b>) LDA scores.</p>
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<p>Non-significant pathways of GSEA analysis results in OS vs. OS + Q comparison groups (nominal <span class="html-italic">p</span> &gt; 0.05).</p>
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22 pages, 6361 KiB  
Article
Comparative Study of the Impacts of Maize and Soybean on Soil and Water Conservation Benefits during Different Growth Stages in the Loess Plateau Region
by Qian Xu, Qingtao Lin and Faqi Wu
Land 2024, 13(8), 1264; https://doi.org/10.3390/land13081264 - 12 Aug 2024
Viewed by 221
Abstract
Maize (Zea mays L.) and soybean (Glycine max L. Merr.) are prevalent summer crops planted widely in the Loess Plateau region of China, which is particularly susceptible to severe soil erosion on the sloping farmland. However, which crop exhibits superior soil [...] Read more.
Maize (Zea mays L.) and soybean (Glycine max L. Merr.) are prevalent summer crops planted widely in the Loess Plateau region of China, which is particularly susceptible to severe soil erosion on the sloping farmland. However, which crop exhibits superior soil and water conservation capabilities while maintaining economic viability, and how their performance in soil and water conservation is affected by slope gradient and rainfall intensity remains unclear. The objective of this study was to compare the impacts of maize and soybean on regulating runoff and sediment through rainfall simulation experiments, and explore the main control factors of soil and water conservation benefits. Five slope gradients (8.7, 17.6, 26.8, 36.4, and 46.6%) and two rainfall intensities (40 and 80 mm h−1) were applied at five respective crop growth stages. Both maize and soybean effectively reduced soil and water losses compared with bare ground, although increasing slope gradient and rainfall intensity weakened the vegetation effect. Compared with slope gradient and rainfall intensity, vegetation coverage was the main factor affecting the performance of maize and soybean in conserving soil and water. The average time delay benefit (TDB), runoff reduction benefit (RRB), and sediment reduction benefit (SRB) of soybean (246.48 ± 11.71, 36.34 ± 2.51, and 54.41 ± 3.42%) were significantly higher (p < 0.05) than those of maize (100.06 ± 6.81, 25.71 ± 1.76, and 43.70 ± 2.91%, respectively) throughout growth. After planting, the increasing rates of vegetation coverage, TDB, RRB, and SRB with time were consistently higher with soybean than maize. Moreover, under the same vegetation coverage, the TDB, RRB, and SRB of soybean were also consistently higher than those of maize. In conclusion, these findings indicate that soybean outperformed maize in terms of soil and water conservation benefits under the experimental conditions, making it more suitable for cultivation on sloping farmland. This finding offers crucial guidance for the cultivation of dry farming in regions plagued by severe soil erosion, facilitating a balance between economic objectives and ecological imperatives. Full article
(This article belongs to the Topic Slope Erosion Monitoring and Anti-erosion)
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<p>The side-spray rainfall simulation system (<b>a</b>) and experimental runoff plots (<b>b</b>) used in this study.</p>
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<p>Average time to runoff (TR), initial loss of rainfall (ILR), runoff volume (RV), and sediment yield (SY) at different growth stages of maize and soybean. BG denotes bare ground; V3, V6, V9, VT, and R2 denote the third leaf, sixth leaf, ninth leaf, tasseling, and blister stages of maize, respectively; V2, V5, R2, R4 and R6 denote the second trifoliolate, fifth trifoliolate, full bloom, full pod, and full seed stages of soybean, respectively; AVG denotes the average value for all growth stages of maize or soybean. Means of each variable category sharing the same lowercase letter are not significantly different (<span class="html-italic">p</span> &gt; 0.05).</p>
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<p>Average time to runoff (<b>a</b>), initial loss of rainfall (<b>b</b>), runoff volume (<b>c</b>), and sediment yield (<b>d</b>) on bare ground, maize plots, and soybean plots on different slope gradients. Maize plots feature maize at the third leaf (V3) to blister (R2) stages, and soybean plots feature soybean at the second trifoliolate (V2) to full seed (S11.1) stages. Means sharing the same lowercase letter are not significantly different at the same slope gradients (<span class="html-italic">p</span> &gt; 0.05).</p>
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<p>Average time to runoff (<b>a</b>), initial loss of rainfall (<b>b</b>), runoff volume (<b>c</b>), and sediment yield (<b>d</b>) on bare ground, maize plots, and soybean plots under different rainfall intensities. Maize plots feature maize at the third leaf (V3) to blister (R2) stages, and soybean plots feature soybean at the second trifoliolate (V2) to full seed (S11.1) stages. Means sharing the same lowercase letter are not significantly different under the same rainfall intensity (<span class="html-italic">p</span> &gt; 0.05).</p>
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<p>Average time delay benefit (TDB), runoff reduction benefit (RRB), and sediment reduction benefit (SRB) of maize and soybean at different growth stages. V3, V6, V9, VT, and R2 denote the third leaf, sixth leaf, ninth leaf, tasseling, and blister stages of maize, respectively; V2, V5, R2, R4 and R6 respectively denote the second trifoliolate, fifth trifoliolate, full bloom, full pod, and full seed stages of soybean, and AVG denotes the average value for all growth stages of maize or soybean. Means of the same benefit category sharing the same lowercase letter are not significantly different at various growth stages (<span class="html-italic">p</span> &gt; 0.05, Duncan’s multiple range test). Means of the same benefit category sharing the same uppercase letter are not significantly different (<span class="html-italic">p</span> &gt; 0.05).</p>
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<p>Average time delay benefit (TDB), runoff reduction benefit (RRB), and sediment reduction benefit (SRB) of maize and soybean on different slope gradients. The growth period spans the third leaf (V3) to blister (R2) stages in the maize plots, and the second trifoliolate (V2) to full seed (S11.1) stages in the soybean plots. Means of each benefit category sharing the same lowercase letter are not significantly different under various slope gradients (<span class="html-italic">p</span> &gt; 0.05).</p>
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<p>Average time delay benefit (TDB), runoff reduction benefit (RRB), and sediment reduction benefit (SRB) of maize and soybean under different rainfall intensities. The growth period spans the third leaf (V3) to blister (R2) stages in the maize plots, and the second trifoliolate (V2) to full seed (S11.1) stages in the soybean plots. Means of each treatment sharing the same lowercase letter are not significantly different under various rainfall intensities (<span class="html-italic">p</span> &gt; 0.05).</p>
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<p>Changes in vegetation coverage, the time delay benefit (TDB), runoff reduction benefit (RRB), and sediment reduction benefit (SRB) of maize and soybean with time.</p>
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<p>Changes in the time delay benefit (TDB), runoff reduction benefit (RRB) and sediment reduction benefit (SRB) of maize and soybean with vegetation coverage.</p>
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<p>Erosion rills found at the base of maize plants (<b>a</b>) and splash erosion pits between plants (<b>b</b>).</p>
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24 pages, 9689 KiB  
Article
Genome-Wide Identification, Evolution, and Expression Analysis of the Dirigent Gene Family in Cassava (Manihot esculenta Crantz)
by Mingchao Li, Kai Luo, Wenke Zhang, Man Liu, Yunfei Zhang, Huling Huang, Yinhua Chen, Shugao Fan and Rui Zhang
Agronomy 2024, 14(8), 1758; https://doi.org/10.3390/agronomy14081758 - 11 Aug 2024
Viewed by 276
Abstract
Dirigent (DIR) genes play a pivotal role in plant development and stress adaptation. Manihot esculenta Crantz, commonly known as cassava, is a drought-resistant plant thriving in tropical and subtropical areas. It is extensively utilized for starch production, bioethanol, and animal feed. [...] Read more.
Dirigent (DIR) genes play a pivotal role in plant development and stress adaptation. Manihot esculenta Crantz, commonly known as cassava, is a drought-resistant plant thriving in tropical and subtropical areas. It is extensively utilized for starch production, bioethanol, and animal feed. However, a comprehensive analysis of the DIR family genes remains unexplored in cassava, a crucial cash and forage crop in tropical and subtropical regions. In this study, we characterize a total of 26 cassava DIRs (MeDIRs) within the cassava genome, revealing their uneven distribution across 13 of the 18 chromosomes. Phylogenetic analysis classified these genes into four subfamilies: DIR-a, DIR-b/d, DIR-c, and DIR-e. Comparative synteny analysis with cassava and seven other plant species (Arabidopsis (Arabidopsis thaliana), poplar (Populus trichocarpa), soybean (Glycine max), tomato (Solanum lycopersicum), rice (Oryza sativa), maize (Zea mays), and wheat (Triticum aestivum)) provided insights into their likely evolution. We also predict protein interaction networks and identify cis-acting elements, elucidating the functional differences in MeDIR genes. Notably, MeDIR genes exhibited specific expression patterns across different tissues and in response to various abiotic and biotic stressors, such as pathogenic bacteria, cadmium chloride (CdCl2), and atrazine. Further validation through quantitative real-time PCR (qRT-PCR) confirmed the response of DIR genes to osmotic and salt stress. These findings offer a comprehensive resource for understanding the characteristics and biological functions of MeDIR genes in cassava, enhancing our knowledge of plant stress adaptation mechanisms. Full article
12 pages, 1708 KiB  
Article
Fabricating High Strength Bio-Based Dynamic Networks from Epoxidized Soybean Oil and Poly(Butylene Adipate-co-Terephthalate)
by Bin Xu, Zhong-Ming Xia, Rui Zhan and Ke-Ke Yang
Polymers 2024, 16(16), 2280; https://doi.org/10.3390/polym16162280 - 11 Aug 2024
Viewed by 378
Abstract
Amid the rapid development of modern society, the widespread use of plastic products has led to significant environmental issues, including the accumulation of non-degradable waste and extensive consumption of non-renewable resources. Developing healable, recyclable, bio-based materials from abundant renewable resources using diverse dynamic [...] Read more.
Amid the rapid development of modern society, the widespread use of plastic products has led to significant environmental issues, including the accumulation of non-degradable waste and extensive consumption of non-renewable resources. Developing healable, recyclable, bio-based materials from abundant renewable resources using diverse dynamic interactions attracts increasing global attention. However, achieving a good balance between the self-healing capacity and mechanical performance, such as strength and toughness, remains challenging. In our study, we address this challenge by developing a new type of dynamic network from epoxidized soybean oil (ESO) and poly(butylene adipate-co-terephthalate) (PBAT) with good strength and toughness. For the synthetic strategy, a thiol–epoxy click reaction was conducted to functionalize ESO with thiol and hydroxyl groups. Subsequently, a curing reaction with isocyanates generated dynamic thiourethane and urethane bonds with different bonding energies in the dynamic networks to reach a trade-off between dynamic features and mechanical properties; amongst these, the thiourethane bonds with a lower bonding energy provide good dynamic features, while the urethane bonds with a higher bonding energy ensure good mechanical properties. The incorporation of flexible PBAT segments to form the rational multi-phase structure with crystalline domains further enhanced the products. A typical sample, OTSO100-PBAT100, exhibited a tensile strength of 33.2 MPa and an elongation at break of 1238%, demonstrating good healing capacity and desirable mechanical performance. This study provides a promising solution to contemporary environmental and energy challenges by developing materials that combine mechanical and repair properties. It addresses the specific gap of achieving a trade-off between tensile strength and elongation at break in bio-based self-healing materials, promising a wide range of applications. Full article
(This article belongs to the Section Biomacromolecules, Biobased and Biodegradable Polymers)
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<p>(<b>A</b>) Synthetic route, (<b>B</b>) 1H NMR spectrum, and (<b>C</b>) FT-IR spectrum of OTSO.</p>
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<p>DSC curves of OTSO−based networks and PBAT: (<b>A</b>) cooling scan and (<b>B</b>) heating scan with a scanning rate of 10 °C/min.</p>
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<p>Stress relaxation curves of (<b>A</b>) OTSO<sub>100</sub>-PBAT<sub>100</sub> and (<b>B</b>) TSO<sub>100</sub>-IPDI<sub>100</sub>, and their apparent activation energy (<b>C</b>,<b>D</b>), respectively.</p>
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<p>(<b>A</b>) Stress–strain curves of samples. (<b>B</b>) Comparison of mechanical properties of this work with relevant references as noted. [<a href="#B42-polymers-16-02280" class="html-bibr">42</a>,<a href="#B45-polymers-16-02280" class="html-bibr">45</a>,<a href="#B46-polymers-16-02280" class="html-bibr">46</a>,<a href="#B48-polymers-16-02280" class="html-bibr">48</a>,<a href="#B49-polymers-16-02280" class="html-bibr">49</a>,<a href="#B50-polymers-16-02280" class="html-bibr">50</a>,<a href="#B51-polymers-16-02280" class="html-bibr">51</a>] (<b>C</b>) Self-healing processes of OTSO<sub>100</sub>-PBAT<sub>100</sub> at 120 °C within 2.5 h.</p>
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23 pages, 21420 KiB  
Article
Design of a Leaf-Bottom Pest Control Robot with Adaptive Chassis and Adjustable Selective Nozzle
by Dongshen Li, Fei Gao, Zemin Li, Yutong Zhang, Chuang Gao and Hongbo Li
Agriculture 2024, 14(8), 1341; https://doi.org/10.3390/agriculture14081341 - 11 Aug 2024
Viewed by 356
Abstract
Pest control is an important guarantee for agricultural production. Pests are mostly light-avoiding and often gather on the bottom of crop leaves. However, spraying agricultural machinery mostly adopts top-down spraying, which suffers from low pesticide utilization and poor insect removal effect. Therefore, the [...] Read more.
Pest control is an important guarantee for agricultural production. Pests are mostly light-avoiding and often gather on the bottom of crop leaves. However, spraying agricultural machinery mostly adopts top-down spraying, which suffers from low pesticide utilization and poor insect removal effect. Therefore, the upward spraying mode and intelligent nozzle have gradually become the research hotspot of precision agriculture. This paper designs a leaf-bottom pest control robot with adaptive chassis and adjustable selective nozzle. Firstly, the adaptive chassis is designed based on the MacPherson suspension, which uses shock absorption to drive the track to swing within a 30° angle. Secondly, a new type of cone angle adjustable selective nozzle was developed, which achieves adaptive selective precision spraying under visual guidance. Then, based on a convolutional block attention module (CBAM), the multi-CBAM-YOLOv5s network model was improved to achieve a 70% recognition rate of leaf-bottom spotted bad point in video streams. Finally, functional tests of the adaptive chassis and the adjustable selective spraying system were conducted. The data indicate that the adaptive chassis can adapt to diverse single-ridge requirements of soybeans and corn while protecting the ridge slopes. The selective spraying system achieves 70% precision in pesticide application, greatly reducing the use of pesticides. The scheme explores a ridge-friendly leaf-bottom pest control plan, providing a technical reference for improving spraying effect, reducing pesticide usage, and mitigating environmental pollution. Full article
(This article belongs to the Section Agricultural Technology)
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<p>Leaf-bottom pest control robot. The red arrow points to the direction of travel.</p>
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<p>Circuit diagram and information flow of the robot.</p>
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<p>The schematic of the chassis deformation and static analysis.</p>
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<p>Schematic diagram of the adaptive module with reference lines marked. <b>A</b>, <b>B</b> is the rotation axis; <b>C</b>, <b>D</b> is the lower and upper fulcrum.</p>
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<p>(<b>a</b>) Curve of shock absorption length changing with swing angle at different installation angles; (<b>b</b>) curves of the slope of the shock absorption length change in three cases.</p>
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<p>The modelling and physical model of the adjustable selective spraying system. (<b>a-i</b>) and (<b>a-ii</b>) are 3D modeling diagrams; (<b>b</b>) is a physical connection diagram of the circulation loop and the water pump; (<b>c</b>) is a physical diagram of the selective adjustable nozzle.</p>
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<p>The pesticide application flowchart.</p>
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<p>The parameter adjustment function schematic.</p>
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<p>The multi-CBAM-YOLOv5s structure.</p>
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<p>CBAM structure: channel attention mechanism and spatial attention mechanism.</p>
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<p>Image preprocessing. The red box is the recognition box.</p>
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<p>Comparison between multi-CBAM-YOLOv5s and basic network model. Model a is Basic-YOLOv5s trained for 40 rounds; Model b is multi-CBAM-YOLOv5s trained for 40 rounds; Model c is multi-CBAM-YOLOv5s trained for 100 rounds. <b>1</b> is the Precision-Confidence curve; <b>2</b> is the Precision-Recall curve; <b>3</b> is the Recall-Confidence curve; the curve of <b>F1</b> and confidence represents the comprehensive score.</p>
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<p>The Adams motion simulation of the adaptive chassis. (<b>a</b>) is a traveling simulation test in Adams; (<b>b</b>) is a real machine simulation test in the laboratory; (<b>c</b>) is a static schematic diagram of the machine on the ridge slope.</p>
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<p>Adaptive chassis swing angle and slope angle change curve diagram.</p>
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<p>Initial angle of shock absorbers and response curves during deformation. Sub-figures (<b>a</b>–<b>c</b>) are from the Adams simulation test. Different initial installation angles correspond to different response speeds. The curves correspond to the Adams screenshots.</p>
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<p>The physical test of the adaptive chassis. (<b>a</b>–<b>c</b>) are the chassis conditions of the actual machine under different working conditions. (<b>d</b>,<b>e</b>) are the chassis working conditions of the actual machine in the test field.</p>
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<p>The recognition results of the leaf database defect points and field test. (<b>a</b>) is the recognition situation of the image stream; (<b>b</b>) is a video screenshot of the real-time recognition of the field video stream.</p>
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<p>The recognition results of the leaf database defect points and field test. (<b>a</b>) is the recognition situation of the image stream; (<b>b</b>) is a video screenshot of the real-time recognition of the field video stream.</p>
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<p>The recognition results of the simulated complex environment. (<b>a</b>) is the original picture; (<b>b</b>) is the image binarization; (<b>c</b>) is the recognition situation of multi-CBAM-YOLOv5s; (<b>d</b>) is the recognition situation of the basic network.</p>
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<p>The real-life spraying tests. Sub-image (<b>a</b>) shows the nozzle’s adjustment of spray parameters; (<b>b</b>) is a screenshot of the actual spraying.</p>
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21 pages, 3203 KiB  
Article
Stratified Effects of Tillage and Crop Rotations on Soil Microbes in Carbon and Nitrogen Cycles at Different Soil Depths in Long-Term Corn, Soybean, and Wheat Cultivation
by Yichao Shi, Alison Claire Gahagan, Malcolm J. Morrison, Edward Gregorich, David R. Lapen and Wen Chen
Microorganisms 2024, 12(8), 1635; https://doi.org/10.3390/microorganisms12081635 - 10 Aug 2024
Viewed by 347
Abstract
Understanding the soil bacterial communities involved in carbon (C) and nitrogen (N) cycling can inform beneficial tillage and crop rotation practices for sustainability and crop production. This study evaluated soil bacterial diversity, compositional structure, and functions associated with C-N cycling at two soil [...] Read more.
Understanding the soil bacterial communities involved in carbon (C) and nitrogen (N) cycling can inform beneficial tillage and crop rotation practices for sustainability and crop production. This study evaluated soil bacterial diversity, compositional structure, and functions associated with C-N cycling at two soil depths (0–15 cm and 15–30 cm) under long-term tillage (conventional tillage [CT] and no-till [NT]) and crop rotation (monocultures of corn, soybean, and wheat and corn–soybean–wheat rotation) systems. The soil microbial communities were characterized by metabarcoding the 16S rRNA gene V4–V5 regions using Illumina MiSeq. The results showed that long-term NT reduced the soil bacterial diversity at 15–30 cm compared to CT, while no significant differences were found at 0–15 cm. The bacterial communities differed significantly at the two soil depths under NT but not under CT. Notably, over 70% of the tillage-responding KEGG orthologs (KOs) associated with C fixation (primarily in the reductive citric acid cycle) were more abundant under NT than under CT at both depths. The tillage practices significantly affected bacteria involved in biological nitrogen (N2) fixation at the 0–15 cm soil depth, as well as bacteria involved in denitrification at both soil depths. The crop type and rotation regimes had limited effects on bacterial diversity and structure but significantly affected specific C-N-cycling genes. For instance, three KOs associated with the Calvin–Benson cycle for C fixation and four KOs related to various N-cycling processes were more abundant in the soil of wheat than in that of corn or soybean. These findings indicate that the long-term tillage practices had a greater influence than crop rotation on the soil bacterial communities, particularly in the C- and N-cycling processes. Integrated management practices that consider the combined effects of tillage, crop rotation, and crop types on soil bacterial functional groups are essential for sustainable agriculture. Full article
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<p>Soil bacterial community diversity indices affected by the interaction of tillage and soil depth (<b>A</b>) and the interaction of tillage, rotation, and crops at 0–15 cm and 15–30 cm soil depths (<b>B</b>). CT, conventional tillage; NT, no-till; C, corn; S, soybean; W, wheat. Error bars represent standard errors. Different letters across all treatments in panel A and across all treatments under CT and NT in panel B represent significant differences at α = 0.05 according to Sidak adjustments.</p>
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<p>Soil bacterial community structure affected by tillage, rotation, crop type, soil depths, and soil physicochemical properties determined by db-RDA. Soil bacterial community structure affected by the interaction of tillage and soil depth (<b>A</b>), the interaction of tillage and crops (<b>B</b>), and all factors (<b>C</b>), and soil bacterial phyla affected by tillage at 0–15 cm (<b>D</b>) and 15–30 cm soil depths (<b>E</b>) and by depth under NT (<b>F</b>). CT, conventional tillage; NT, no-till; 0–15, 0–15 cm soil depth; 15–30, 15–30 cm soil depth. Error bars represent standard errors.</p>
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<p>Functional genes associated with carbon fixation, as predicted by PICRUSt2. The relative abundances of KEGG orthologs (KOs) that were significantly influenced by the tillage practices at either 0–15 cm or 15–30 cm soil depth within four key modules from map00720 (other C fixation pathways except Calvin–Benson cycle) are shown, including the reductive citric acid cycle (M00173), the hydroxypropionate–hydroxybutylate cycle (M00375), the 3-hydroxypropionate bi-cycle (M00376), and the Wood–Ljungdahl pathway (M00377) (<b>A</b>). Additionally, KOs within the Calvin–Benson cycle module (M00165) were significantly influenced by the crop types (<b>C</b>). The corresponding enzyme IDs (EC) for each KO are provided on the right side of the panels. Different letters across all treatments in panels (<b>A</b>,<b>C</b>) represent significant differences at α = 0.05 according to Sidak adjustments. Panels (<b>B</b>,<b>D</b>) display heatmaps showing the abundance of bacterial genera significantly affected by tillage (<b>B</b>) or crop types (<b>D</b>), which importantly contributed to the KOs presented in panels (<b>A</b>,<b>C</b>), respectively. Colors and density represent the proportion (%) of a specific gene’s abundance within various genera, with blue indicating lower abundance, and red indicating higher abundance. CT, conventional tillage; NT, no-till; 0–15, 0–15 cm soil depth; 15–30, 15–30 cm soil depth. Crop types: C for corn, S for soybean, and W for wheat.</p>
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<p>Functional genes associated with nitrogen (N) metabolism, as predicted by PICRUSt2. Panels (<b>A</b>,<b>B</b>,<b>E</b>) illustrate the relative abundance of KEGG orthologs (KOs) significantly influenced by the tillage practices at 0–15 cm soil depth and 15–30 cm soil depth and the current crop, respectively. Different letters (a, b, c) above the bars in panel (<b>E</b>) represent significant differences between the crops at α = 0.05 according to Sidak adjustments. Results for crops sharing the same letter are not significantly different from each other, while those for crops with different letters are significantly different. Panels (<b>C</b>,<b>D</b>,<b>F</b>) display heatmaps showing the abundance of bacterial genera that significantly contributed to the KOs affected by the tillage practices at 0–15 cm soil depth (<b>C</b>) and 15–30 cm soil depth (<b>D</b>) and the current crop (<b>F</b>). Stars in the heatmaps indicate significant differences in the abundance of genera between conventional tillage (CT) and no-till (NT) in panels (<b>C</b>,<b>D</b>), and higher abundance in wheat compared to corn or soybean in panel (<b>F</b>) (*, <span class="html-italic">p</span> ≤ 0.05). Different colors represent the proportion (%) of a specific gene’s abundance within various genera. CT, conventional tillage; NT, no-till; C, corn; S, soybean; W, wheat. Error bars represent standard errors.</p>
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20 pages, 7272 KiB  
Article
Preparation of Supercapacitor Carbon Electrode Materials by Low-Temperature Carbonization of High-Nitrogen-Doped Raw Materials from Food Waste
by Qingnan Mu, Chang Liu, Yao Guo, Kun Wang, Zhijie Gao, Yuhan Du, Changqing Cao, Peigao Duan and Krzysztof Kapusta
Materials 2024, 17(16), 3984; https://doi.org/10.3390/ma17163984 - 10 Aug 2024
Viewed by 310
Abstract
To address the problem of the low nitrogen (N) content of carbon materials prepared through the direct carbonization of food waste, soybean meal and egg whites with high N contents were selected to carry out carbonization experiments on food waste. At 220 °C, [...] Read more.
To address the problem of the low nitrogen (N) content of carbon materials prepared through the direct carbonization of food waste, soybean meal and egg whites with high N contents were selected to carry out carbonization experiments on food waste. At 220 °C, the effects of hydrothermal carbonization and microwave carbonization on the properties of supercapacitor electrode materials were investigated. The results show that food waste doped with soybean meal and egg whites could achieve good N doping. At a current density of 1 A·g−1, the specific capacitance of the doped carbon prepared by hydrothermal doping is as high as 220.00 F·g−1, which is much greater than that of the raw material prepared through the hydrothermal carbonization of food waste alone, indicating that the hydrothermal carbonization reactions of soybean meal, egg white, and food waste promote the electrochemical properties of the prepared carbon materials well. However, when a variety of raw materials are mixed for pyrolysis carbonization, different raw materials cannot be fully mixed in the pyrolysis process, and under the etching action of potassium hydroxide, severe local etching and local nonetching occur, resulting in a severe increase in the pore size distribution and deterioration of the electrochemical performance of the prepared carbon materials. At a current density of 1 A·g−1, the specific capacitance of these prepared carbon materials is 157.70 F·g−1, whereas it is only 62.00 F·g−1 at a high current density of 20 A·g−1. Therefore, this study suggests that the hydrothermal carbonization process is superior to the microwave pyrolysis carbonization process for preparing supercapacitor electrode materials with multiple samples doped with each other. Full article
(This article belongs to the Special Issue Recycling and Resource Utilization of Waste)
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<p>Distribution of yields of doped hydrothermal carbonization products.</p>
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<p>SEM images of (<b>a</b>) HFS; (<b>b</b>) AHFS; (<b>c</b>) HFE; (<b>d</b>) AHFE; (<b>e</b>) HFSE; (<b>f</b>) AHFSE; (<b>g</b>) MFSE; and (<b>h</b>) and AMFSE.</p>
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<p>BET test results for AHFE, AMFSE, AHFSE, and AHFS. (<b>a</b>). N<sub>2</sub> adsorption/desorption curve; (<b>b</b>). Pore size distribution curve.</p>
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<p>Binding energy profiles for AHFS, AHFE, and AHSE: (<b>a</b>,<b>b</b>) C1s and N1s spectra of AHFS; (<b>c</b>,<b>d</b>) C1s and N1s of AHFEs; (<b>e</b>,<b>f</b>) C1s and N1s of the AHSE.</p>
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<p>Binding energy profiles for AHFSE and AMFSE: (<b>a</b>,<b>b</b>) C1s and N1s of AHFSE; (<b>c</b>,<b>d</b>) C1s and N1s of AMFSE.</p>
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<p>Electrochemical properties of the AHS and AHE. (<b>a</b>) CV curves of AHS, AHE, and AHC−220. (<b>b</b>) GCD curves of AHS, AHE, and AHC−220. (<b>c</b>) Specific capacitance curves at different current densities. (<b>d</b>) Nyquist impedance plots.</p>
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<p>Electrochemical properties of AHFS, AHFE, AHSE, and AHFSE. (<b>a</b>) CV curves obtained at 10 mV/s. (<b>b</b>) GCD curves obtained at 1 A/g. (<b>c</b>) Specific capacitance curves obtained at different current densities. (<b>d</b>) Nyquist impedance plots.</p>
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<p>Specific capacitance of experimental samples of FW, SM, and EW hydrothermally doped at current densities of 1 A/g and 20 A/g and capacitance retention at high current densities.</p>
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<p>Electrochemical properties of AMFSE. (<b>a</b>) CV curves obtained at different current densities. (<b>b</b>) GCD curves (obtained at different current densities). (<b>c</b>) Specific capacitance curves obtained at different current densities. (<b>d</b>) Nyquist impedance plots.</p>
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21 pages, 2150 KiB  
Article
Comparative Life Cycle Assessment of Sustainable Aviation Fuel Production from Different Biomasses
by Fabrizio D’Ascenzo, Giuliana Vinci, Marco Savastano, Aurora Amici and Marco Ruggeri
Sustainability 2024, 16(16), 6875; https://doi.org/10.3390/su16166875 (registering DOI) - 10 Aug 2024
Viewed by 418
Abstract
The aviation sector makes up 11% of all transportation emissions and is considered a “hard to abate” sector since, due to the long distances to be traveled, opportunities for electrification are rather limited. Therefore, since there are no alternatives to fuels, Sustainable Aviation [...] Read more.
The aviation sector makes up 11% of all transportation emissions and is considered a “hard to abate” sector since, due to the long distances to be traveled, opportunities for electrification are rather limited. Therefore, since there are no alternatives to fuels, Sustainable Aviation Fuels (SAFs), or fuels produced from biomass, have recently been developed to reduce climate-changing emissions in the aviation sector. Using Life Cycle Assessment, this research evaluated the environmental compatibility of different SAF production routes from seven biomasses: four food feedstocks (Soybean, Palm, Rapeseed, and Camelina), one non-food feedstock (Jatropha curcas L.), and two wastes (Waste Cooking Oil, or WCO, and Tallow). The evaluation was carried out using SimaPro 9.5 software. The results showed that the two potentially most favorable options could be Camelina and Palma, as they show minimal environmental impacts in 4 and 7 out of 18 impact categories, respectively. Soybean, on the other hand, appears to be the least sustainable precursor. Considering GWP, SAF production could reduce the values compared to fossil fuel by 2.8–3.6 times (WCO), 1.27–1.66 times (Tallow), 4.6–5.8 times (Palm), 3.4–4.3 times (Jatropha), 1.05–1.32 times (Rapeseed), and 4.36–5.5 times (Camelina), demonstrating the good environmental impact of these pathways. Finally, the sensitivity analysis showed that SAF production from waste could be an environmentally friendly option, with rather low environmental impacts, in the range of 5.13 g CO2 eq/MJ for Tallow and 3.12 g CO2 eq/MJ for WCO. However, some of the energy would have to come from sustainable energy carriers such as biomethane and renewable sources such as photovoltaic energy. Full article
(This article belongs to the Section Economic and Business Aspects of Sustainability)
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<p>Global CO<sub>2</sub> emissions from the transport sector by subsector [<a href="#B3-sustainability-16-06875" class="html-bibr">3</a>].</p>
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<p>Literature trend on “Life Cycle Assessment AND Sustainable Aviation Fuels” (Scopus) (Abs-Keywords-Title) (June 2024).</p>
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<p>SA results for food biomass. S1 = baseline scenario; S2 = 20% solar energy; S3 = 20% solar energy + biomethane. (<b>A</b>) = Camelina; (<b>B</b>) = Soybean; (<b>C</b>) = Rapeseed; (<b>D</b>) = Palm (GWP = Global Warming Potential; SOD = Stratospheric Ozone Depletion; IR = Ionizing Radiation; OFHH = Ozone Formation, Human Health; FPMP = Fine Particulate Matter Formation; OFTE = Ozone Formation, Terrestrial Ecosystems; TAP = Terrestrial Acidification Potential (TAP); FEP = Freshwater Eutrophication Potential; MEP = Marine Eutrophication Potential; TEC = Terrestrial Ecotoxicity; FEC = Freshwater Ecotoxicity; MEC = Marine Ecotoxicity; HCT = Human Carcinogenic Toxicity; HNCT = Human Non-Carcinogenic Toxicity; LU = Land Use; MRS = Mineral Resources Scarcity; FRS = Fossil Resources Scarcity; WC = Water Consumption.</p>
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<p>SA results for non-food biomass (<span class="html-italic">Jatropha curcas</span> L.). S1 = baseline scenario; S2 = 20% solar energy; S3 = 20% solar energy + biomethane (GWP = Global Warming Potential; SOD = Stratospheric Ozone Depletion; IR = Ionizing Radiation; OFHH = Ozone Formation, Human Health; FPMP = Fine Particulate Matter Formation; OFTE = Ozone Formation, Terrestrial Ecosystems; TAP = Terrestrial Acidification Potential (TAP); FEP = Freshwater Eutrophication Potential; MEP = Marine Eutrophication Potential; TEC = Terrestrial Ecotoxicity; FEC = Freshwater Ecotoxicity; MEC = Marine Ecotoxicity; HCT = Human Carcinogenic Toxicity; HNCT = Human Non-Carcinogenic Toxicity; LU = Land Use; MRS = Mineral Resources Scarcity; FRS = Fossil Resources Scarcity; WC = Water Consumption.</p>
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<p>SA results for non-food biomass (<span class="html-italic">Jatropha curcas</span> L.). S1 = baseline scenario; S2 = 20% solar energy; S3 = 20% solar energy + biomethane. (<b>A</b>) = Tallow; (<b>B</b>) = Waste Cooking Oil (GWP = Global Warming Potential; SOD = Stratospheric Ozone Depletion; IR = Ionizing Radiation; OFHH = Ozone Formation, Human Health; FPMP = Fine Particulate Matter Formation; OFTE = Ozone Formation, Terrestrial Ecosystems; TAP = Terrestrial Acidification Potential (TAP); FEP = Freshwater Eutrophication Potential; MEP = Marine Eutrophication Potential; TEC = Terrestrial Ecotoxicity; FEC = Freshwater Ecotoxicity; MEC = Marine Ecotoxicity; HCT = Human Carcinogenic Toxicity; HNCT = Human Non-Carcinogenic Toxicity; LU = Land Use; MRS = Mineral Resources Scarcity; FRS = Fossil Resources Scarcity; WC = Water Consumption.</p>
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17 pages, 18568 KiB  
Article
A Mixture of Soybean Oil and Lard Alleviates Postpartum Cognitive Impairment via Regulating the Brain Fatty Acid Composition and SCFA/ERK(1/2)/CREB/BDNF Pathway
by Runjia Shi, Xiaoying Tian, Andong Ji, Tianyu Zhang, Huina Xu, Zhongshi Qi, Liying Zhou, Chunhui Zhao and Duo Li
Nutrients 2024, 16(16), 2641; https://doi.org/10.3390/nu16162641 - 10 Aug 2024
Viewed by 391
Abstract
Lard is highly appreciated for its flavor. However, it has not been elucidated how to consume lard while at the same time eliminating its adverse effects on postpartum cognitive function. Female mice were divided into three groups (n = 10): soybean oil [...] Read more.
Lard is highly appreciated for its flavor. However, it has not been elucidated how to consume lard while at the same time eliminating its adverse effects on postpartum cognitive function. Female mice were divided into three groups (n = 10): soybean oil (SO), lard oil (LO), and a mixture of soybean oil and lard at a ratio of 1:1 (LS). No significant difference was observed between the SO and LS groups in behavioral testing of the maternal mice, but the LO group was significantly worse compared with these two groups. Moreover, the SO and LS supplementation increased docosahexaenoic acid (DHA) and total n-3 polyunsaturated fatty acid (PUFA) levels in the brain and short-chain fatty acid (SCFA)-producing bacteria in feces, thereby mitigating neuroinflammation and lowering the p-ERK(1/2)/ERK(1/2), p-CREB/CREB, and BDNF levels in the brain compared to the LO group. Collectively, the LS group inhibited postpartum cognitive impairment by regulating the brain fatty acid composition, neuroinflammation, gut microbiota, and the SCFA/ERK(1/2)/CREB/BDNF signaling pathway compared to lard. Full article
(This article belongs to the Section Lipids)
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<p>The schematic depicting the present study design.</p>
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<p>Effect of a mixture of soybean oil and lard on postpartum cognitive function. (<b>A</b>–<b>C</b>) The detection results and (<b>D</b>–<b>F</b>) the representative traveled path of maternal mice in the open-field test. (<b>G</b>–<b>I</b>) The detection results and (<b>J</b>–<b>L</b>) the representative traveled path of maternal mice in the Y-maze test. (<b>M</b>–<b>O</b>) The detection results and (<b>P</b>–<b>R</b>) the representative traveled path of maternal mice in the Morris water maze test. Data represent mean ± SD (<span class="html-italic">n</span> = 7). * <span class="html-italic">p</span> &lt; 0.05 and ** <span class="html-italic">p</span> &lt; 0.01 represent the significant difference.</p>
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<p>Effect of a mixture of soybean oil and lard on brain fatty acid profile and its correlation with behavioral testing outcome indicators. (<b>A</b>,<b>B</b>) Brain fatty acid proportions (%). (<b>C</b>–<b>H</b>) Pearson’ correlations between DHA and <span class="html-italic">n</span>-3 PUFA levels in the maternal mice brain and behavioral testing outcome indicators. Data represent median (interquartile range) (<span class="html-italic">n</span> = 6). * <span class="html-italic">p</span> &lt; 0.05 and ** <span class="html-italic">p</span> &lt; 0.01 represent the significant difference.</p>
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<p>Effect of a mixture of soybean oil and lard on brain histopathology. (<b>A</b>) The H&amp;E and (<b>B</b>) Nissl staining diagrams in the brain (scale bar = 20 μm). Red arrows: nuclei pyknosis.</p>
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<p>Effect of a mixture of soybean oil and lard on the activation of neuroglial cells. Brain GFAP expression was analyzed via (<b>A</b>) immunofluorescence and (<b>B</b>) Western blotting (<span class="html-italic">n</span> = 4). IBA1 expression in the brain was analyzed via (<b>C</b>) immunofluorescence and (<b>D</b>) Western blotting (<span class="html-italic">n</span> = 4). Scale bar = 20 μm. Data represent median (interquartile range). * <span class="html-italic">p</span> &lt; 0.05 and ** <span class="html-italic">p</span> &lt; 0.01 represent the significant difference.</p>
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<p>Effect of a mixture of soybean oil and lard on brain neuroinflammation. The levels of (<b>A</b>–<b>D</b>) inflammatory cytokines (pg/g, <span class="html-italic">n</span> = 3) and (<b>E</b>–<b>H</b>) NLRP3 inflammasome complex-related proteins (<span class="html-italic">n</span> = 4). Data represent mean ± SD or median (interquartile range). * <span class="html-italic">p</span> &lt; 0.05 and ** <span class="html-italic">p</span> &lt; 0.01 represent the significant difference.</p>
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<p>Effect of a mixture of soybean oil and lard on the gut microbiota composition. (<b>A</b>) Chao1 index; (<b>B</b>) ACE index; (<b>C</b>) Shannon index; (<b>D</b>) Simpson index of each group. (<b>E</b>) Principal coordinates analysis (PCoA) of weighted unifrac. All phyla (<b>F</b>) and genera (<b>G</b>) of gut microbiota. Data represent median (interquartile range) (<span class="html-italic">n</span> = 8). * <span class="html-italic">p</span> &lt; 0.05 represents the significant difference.</p>
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<p>Effect of a mixture of soybean oil and lard on the gut microbiota composition. The (<b>A</b>) cladogram (LDA &gt; 3) and (<b>B</b>) LDA score of the taxa obtained from LEfSe analysis. (<b>C</b>) The Pearson correlation analysis between behavioral testing outcome indicators and the biomarkers in microbiota from each group. * <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>Effect of a mixture of soybean oil and lard on the SCFA and ERK(1/2)/CREB/BDNF pathway-related protein levels. (<b>A</b>) The levels of SCFAs in the feces of maternal mice (µg/g, <span class="html-italic">n</span> = 3). (<b>B</b>–<b>F</b>) The relative protein levels of p-ERK(1/2)/ERK(1/2), p-CREB/CREB, BDNF, and PSD-95 in brain (<span class="html-italic">n</span> = 4). (<b>G</b>) The ultrastructure of synapses on the transmission electron micrograph in the hippocampus (Scale bars = 2 μm and 1 μm). Red arrows: postsynaptic density. Data represent mean ± SD or median (interquartile range). * <span class="html-italic">p</span> &lt; 0.05 and ** <span class="html-italic">p</span> &lt; 0.01 represent the significant difference.</p>
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13 pages, 12409 KiB  
Article
Effects of Biochar under Different Preparation Conditions on the Growth of Capsicum
by Haiwei Xie, Xuan Zhou and Yan Zhang
Sustainability 2024, 16(16), 6869; https://doi.org/10.3390/su16166869 (registering DOI) - 10 Aug 2024
Viewed by 401
Abstract
Biochar return to the field has been widely explored, but there is a problematic disconnect between biochar preparation and biochar return to the field. In this study, soybean straw is used as a raw material and is sieved into two components: 60-mesh (0.250 [...] Read more.
Biochar return to the field has been widely explored, but there is a problematic disconnect between biochar preparation and biochar return to the field. In this study, soybean straw is used as a raw material and is sieved into two components: 60-mesh (0.250 mm) and 110-mesh (0.130 mm). Four kinds of biochar were obtained by pyrolysis under the condition of no heat preservation and heat preservation for 60 min. The biochar was applied to the soil, and the effects of biochar on soil and capsicum growth were analyzed by Spearman correlation. Compared with the control group, soil pH, soil electrical conductivity, and soil organic matter decomposition were increased by 0.58, 101 μs/cm, and 9.48%, respectively. The fruit quantity, plant height, water, fat, soluble solid, and titrable acidity of capsicum were increased by 1, 0.55, 0.08, 0.62, 0.67, and 0.7 times, respectively. Spearman correlation analysis showed that soil properties and capsicum growth were most affected by biochar’s specific surface area (SSA). Therefore, increasing the biomass mesh number and heat preservation time is beneficial to increasing the SSA of biochar and facilitating the return of biochar to the field and the best preparation conditions are 110-mesh soybean straw biomass pyrolysis and heat preservation for 60 min. Full article
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<p>The scanning electron microscope pictures of (<b>a</b>) BC60-0 min; (<b>b</b>) BC60-60 min; (<b>c</b>) BC110-0 min; (<b>d</b>) BC110-60 min.</p>
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<p>FT-IR spectra with different preparation conditions.</p>
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<p>Biochar X-ray diffraction (XRD) under preparation conditions.</p>
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<p>Soil pH (<b>a</b>) and EC (<b>b</b>) after application of biochar prepared under different conditions and without biochar application.</p>
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<p>Impact of biochar application on soil organic matter of soil generated at different preparation conditions.</p>
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<p>(<b>a</b>) Growth of plants in 5 treatments; (<b>b</b>) Number of fruits during planting; (<b>c</b>) Plant length.</p>
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<p>Effects of biochar under different preparation conditions on the (<b>a</b>) hydration, (<b>b</b>) fat, (<b>c</b>) soluble solid, and (<b>d</b>) titrable acid of capsicum fruit.</p>
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