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14 pages, 1213 KiB  
Article
Impact of High-Pressure Processing on Quality and Safety of High-Oil-Content Pesto Sauce: A Comparative Study with Thermal Processing
by Ehsan Shad, Kaisa Raninen, Svetlana Podergina, Lok In Chan, Kam Pui Tong, Heidi Hälikkä, Marjo Huovinen and Jenni Korhonen
Appl. Sci. 2024, 14(20), 9425; https://doi.org/10.3390/app14209425 (registering DOI) - 16 Oct 2024
Viewed by 175
Abstract
High-pressure processing (HPP) is a promising technology for increasing the shelf life of food, with minimal effects on the nutritional or sensory quality. However, there has been a concern that high-oil-content foods may protect food pathogens in HPP, and that HPP can affect [...] Read more.
High-pressure processing (HPP) is a promising technology for increasing the shelf life of food, with minimal effects on the nutritional or sensory quality. However, there has been a concern that high-oil-content foods may protect food pathogens in HPP, and that HPP can affect the quality of lipids. We inoculated Listeria monocytogenes and Salmonella Typhimurium into 34% and 54% oil-content pesto sauce, processed them either with HPP (600 MPa, 4 min) or thermal processing (82 °C, 5 min), and analyzed bacteria counts, pH, GC-MS (Terpene compounds), the time–kill kinetic study, and lipid oxidation value for 60 days in refrigerating storage (5 ± 2 °C). Our findings show that HPP significantly reduced the number of bacteria (more than 4-log) compared to thermal processing or non-processing. Additionally, we discovered terpene compounds (highest-level terpene: L-linalool, eugenol, and 1,8-cineol) in pesto oil that exhibit antimicrobial activity. Different oil content did not have any significant effect on bacteria levels. Regarding chemical results, all samples were of acceptable quality, and the processes did not show any negative effect on lipid oxidation (Peroxide and P-Anisidine value under 10 meq per kilogram of oil). In conclusion, our study indicates that HPP is a suitable method for high-oil-content pesto sauce. In addition, functional compounds naturally present in pesto may contribute to maintaining its microbial and chemical quality. Full article
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<p><span class="html-italic">Listeria monocytogenes</span> counts in the pesto samples having 34 or 54% oil content and different processing methods on day 1 (<b>a</b>) and during 60 days of storage (<b>b</b>). Data represent the mean ± standard deviation of three independent replicates; different superscript letters in each column indicate significant differences (<span class="html-italic">p</span> &lt; 0.05). LNP: non-processed samples with <span class="html-italic">L. monocytogenes,</span> LTP: thermal-processed samples with <span class="html-italic">L. monocytogenes,</span> LHP: HPP samples with <span class="html-italic">L. monocytogenes.</span> LOD: limit of detection (2 log CFU/g).</p>
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<p>Effect of HPP and thermal processing on pH in pesto sauces. Data represent the mean ± standard deviation of three independent replicates; different superscript letters in each column indicate significant differences (<span class="html-italic">p</span> &lt; 0.05). NP-34: 34% oil-content non-processed sample, HPP-34: 34% oil-content HPP sample, TP-34: 34% oil-content thermal-processed sample, NP-54: 54% oil-content non-processed sample, HPP-54: 54% oil-content HPP sample, TP-54: 54% oil-content thermal-processed sample.</p>
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<p>Effect of HPP and thermal processing on lipid oxidation in pesto sauce. (<b>a</b>) Peroxide value results in day 1 (solid fill) and 60 (pattern fill) of storage time, (<b>b</b>) p-anisidine value results after 60 days storage time. Data represent the mean ± standard deviation of three independent replicates; different superscript letters in each column indicate significant differences (<span class="html-italic">p</span> &lt; 0.05, Same letters mean no significant differences). NP-34: 34% oil-content non-processed sample, HPP-34: 34% oil-content HPP sample, TP-34: 34% oil-content thermal-processed sample, NP-54: 54% oil-content non-processed sample, HPP-54: 54% oil-content HPP sample, TP-54: 54% oil-content thermal-processed sample.</p>
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17 pages, 2642 KiB  
Article
Effects of Temperature and Packaging Atmosphere on Shelf Life, Biochemical, and Sensory Attributes of Glasswort (Salicornia europaea L.) Grown Hydroponically at Different Salinity Levels
by Chiara Sanmartin, Isabella Taglieri, Alessandro Bianchi, Prangthip Parichanon, Martina Puccinelli, Alberto Pardossi and Francesca Venturi
Foods 2024, 13(20), 3260; https://doi.org/10.3390/foods13203260 (registering DOI) - 13 Oct 2024
Viewed by 501
Abstract
Halophytes, such as Salicornia species, are promising new foods and are consumed for their pleasant salty taste and nutritional value. Since Salicornia is perishable, modified atmospheric packaging (MAP) can be a useful tool, in combination with proper temperature, to halt further quality degradation [...] Read more.
Halophytes, such as Salicornia species, are promising new foods and are consumed for their pleasant salty taste and nutritional value. Since Salicornia is perishable, modified atmospheric packaging (MAP) can be a useful tool, in combination with proper temperature, to halt further quality degradation in this type of product. The purpose of this study was to investigate the effect of MAP, with or without refrigeration, to extend the shelf life of glasswort (Salicornia europaea L.) grown hydroponically (floating raft system) in a greenhouse with a nutrient solution containing 0 g/L (C) or 12.5 g/L of NaCl (T). The dry matter content, weight loss, respiration rate, biochemical composition, color, antioxidant capacity, and sensorial attributes were determined in shoots after harvest and during storage in plastic bags filled with technical air or with MAP at 4 or 20 °C for 120 h. At harvest, plants supplied with salt-enriched solution (T) showed a significant improvement in nutritional value and sensory profile. Storage in air at room temperature (20 °C) accelerated weight loss and diminished color stability, particularly in non-salinity samples (C), while MAP extended the shelf life of all the samples regardless of the storage temperature adopted. Optimal storage conditions were observed when MAP was combined with refrigeration, which allowed to effectively preserve shoots sensory acceptability for a period of about seven days. Future research could further explore the long-term effects on the nutritional value and sensory quality of S. europaea under various combinations of MAP and different storage temperatures ranging between 4 °C and 20 °C. Full article
(This article belongs to the Section Food Quality and Safety)
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<p>Sensory parameters evaluated at harvest in <span class="html-italic">Salicornia europaea</span> shoots with different salinity levels (0 g/L NaCl (C) and 12.5 g/L NaCl (T)). Significance level: *** = <span class="html-italic">p</span> &lt; 0.001; ** = <span class="html-italic">p</span> &lt; 0.01; * = <span class="html-italic">p</span> &lt; 0.05; n.s. = not significant (<span class="html-italic">p</span> ≥ 0.05).</p>
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<p>Weight loss during storage time at different temperatures (4 °C and 20 °C) and packaging atmospheres (Air and MAP) of <span class="html-italic">Salicornia europaea</span> shoots with different salinity levels: (<b>a</b>) C (0 g/L NaCl); (<b>b</b>) T (12.5 g/L NaCl).Values are the average (bars indicate ± SD) (n = 3). Different letters in each group indicate statistical differences (Duncan’s MRT, <span class="html-italic">p</span> ≤ 0.05).</p>
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<p>Gas concentration (% O<sub>2</sub> and % CO<sub>2</sub>) inside package during storage at different packaging conditions (Air and MAP) of <span class="html-italic">Salicornia europaea</span> shoots with different salinity levels: (<b>a</b>) 0 g/L stored at 4 °C (C4); (<b>b</b>) 12.5 g/L stored at 4 °C (T4); (<b>c</b>) 0 g/L stored at 20 °C (C20); (<b>d</b>) 12.5 g/L stored at 20 °C (T20). Values are the average (bars indicate ± SD) (n = 3).</p>
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<p>Time (hours) of shelf life end, which the Hedonic Index falls below the acceptability limit (HI ≤ 6) in <span class="html-italic">Salicornia europaea</span> grown in the hydroponic system with different salinity levels (0 g/L (C) and 12.5 g/L (T)) stored at two temperatures (4 °C and 20 °C) and with two packaging atmospheres (Air and MAP). Values are the average (bars indicate ± SD) (n = 3). Different letters indicate significant differences in value (Duncan’s MRT, <span class="html-italic">p</span> ≤ 0.05).</p>
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21 pages, 1136 KiB  
Article
Exploring the Biological Value of Red Grape Skin: Its Incorporation and Impact on Yogurt Quality
by Eugenia Covaliov, Tatiana Capcanari, Vladislav Reșitca, Aurica Chirsanova, Alina Boiștean, Rodica Sturza, Antoanela Patras, Cristina Bianca Pocol, Olga Ruseva and Ana Chioru
Foods 2024, 13(20), 3254; https://doi.org/10.3390/foods13203254 - 13 Oct 2024
Viewed by 315
Abstract
The study was conducted to study the sustainability and enhanced nutrition gains obtained from incorporating grape skin powder (GSP) extracted from both Fetească Neagră and Rară Neagră grape varieties into yogurt. Grape skins are major leftovers from wineries, having high amounts of phenolic [...] Read more.
The study was conducted to study the sustainability and enhanced nutrition gains obtained from incorporating grape skin powder (GSP) extracted from both Fetească Neagră and Rară Neagră grape varieties into yogurt. Grape skins are major leftovers from wineries, having high amounts of phenolic compounds and dietary fiber responsible for their ability to improve the characteristics of food. The research aimed to evaluate the effect of GSP addition at varying concentrations (0.5%, 1.0%, and 1.5%) on the yogurt’s physicochemical properties, antioxidant activity, color parameters, and sensory attributes. Analysis revealed that both Fetească Neagră and Rară Neagră GSP increased the total phenolic content and antioxidant activity; however, Fetească Neagră showed greater improvements, with TPC reaching 1.52 mg GAE/100 g and DPPH inhibition up to 26.63%. Although slightly lower, TPC rose to 1.43 mg GAE/100 g and DPPH inhibition increased to 18.93% with Rară Neagră enhancing these parameters conversely. Color changes were observed in fortified yogurts where lightness decreased (L*) and redness increased (a*) due to the pH-dependent anthocyanin stability. Syneresis, indicative of yogurt’s water-holding capacity, was reduced at higher concentrations of GSP from both varieties, suggesting improved textural integrity. Sensory evaluation indicated that consumers generally favored yogurts with lower concentrations of GSP. Yogurts fortified with Fetească Neagră GSP received higher overall preference, while those with Rară Neagră GSP were also well-received for their distinct flavor profiles when used at suitable levels. These results show that GSP from both types of grapes improves the nutritional value of yogurt and complies with the principles of sustainable food production through reutilizing agro-industrial waste. Full article
(This article belongs to the Section Dairy)
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<p>Impact of Fetească Neagră and Rară Neagră GSP concentration on total phenolic content and antioxidant activity of yogurt.</p>
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<p>Mean ranking of yogurt enriched with different grape skin powder level. Control—control sample, FN0.5—yogurt with 0.5% addition of Fetească Neagră grape skin powder, FN1.0—yogurt with 1.0% addition of Fetească Neagră grape skin powder, FN1.5—yogurt with 1.5% addition of Fetească Neagră grape skin powder, RN0.5—yogurt with 0.5% addition of Rară Neagră grape skin powder, RN1.0—yogurt with 1.0% addition of Rară Neagră grape skin powder, RN1.5—yogurt with 1.5% addition of Rară Neagră grape skin powder.</p>
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<p>Logistic regression of yogurt purchase decision by Fetească Neagră GSP concentration.</p>
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<p>Logistic regression of yogurt purchase decision by Rară Neagră GSP concentration.</p>
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<p>Visualized results of the check-all-that-apply (CATA) analysis of the seven yogurt samples. Control—control sample, FN0.5—yogurt with 0.5% addition of Fetească Neagră grape skin powder, FN1.0—yogurt with 1.0% addition of Fetească Neagră grape skin powder, FN1.5—yogurt with 1.5% addition of Fetească Neagră grape skin powder, RN0.5—yogurt with 0.5% addition of Rară Neagră grape skin powder, RN1.0—yogurt with 1.0% addition of Rară Neagră grape skin powder, RN1.5—yogurt with 1.5% addition of Rară Neagră grape skin powder.</p>
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13 pages, 840 KiB  
Article
Assessment of the Antioxidative Properties of Extracts from the Fruits of Pyrus pyraster (L.) Burgsd and Pyrus ×myloslavensis Czarna & Antkowiak Grown under Natural Environmental Conditions
by Marzanna Hęś, Wojciech Antkowiak, Kinga Stuper-Szablewska, Krzysztof Dziedzic, Marta Jessa and Paulina Ratajczak
Agriculture 2024, 14(10), 1790; https://doi.org/10.3390/agriculture14101790 - 12 Oct 2024
Viewed by 227
Abstract
Analyses were conducted on extracts from the fruits of P. pyraster and P. ×myloslavensis. Extraction with 80% methanol was performed at room temperature. The total phenolic content was determined by spectrophotometry using the Folin–Ciocalteu reagent, with gallic acid as the reference [...] Read more.
Analyses were conducted on extracts from the fruits of P. pyraster and P. ×myloslavensis. Extraction with 80% methanol was performed at room temperature. The total phenolic content was determined by spectrophotometry using the Folin–Ciocalteu reagent, with gallic acid as the reference standard. Phenolic compounds and organic acids were identified on a liquid chromatograph. The antioxidative activity of the extracts was tested in relation to linoleic acid incubation of the emulsions for 19 h based on the neutralization of the DPPH radical (2,2-diphenyl-1-picrylhydrazyl) and the ABTS cation radical (2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid]) as well as by the ferric reducing antioxidant power (FRAP) assay. The analyses showed that the extract from P. pyraster fruits is characterized by a higher content of phenolic compounds and a higher antioxidative potential compared with that from P. ×myloslavensis. In extracts of both pear species, seven phenolic compounds and four organic acids were identified. The total fiber content in pears of P. pyraster and P. ×myloslavensis was determined at 36.45 g and 24.74 g/100 g d.m. of the pear fruits, of which most comprised the insoluble fraction (32.49 g and 20.86/100 g, respectively). The results of the conducted research are highly significant, as they confirm that pears contain many valuable nutrients and biologically active compounds, including antioxidants and dietary fiber. Adding pear extracts to food products may offer a way to boost their health benefits while also broadening the variety of items that have appealing sensory characteristics. Moreover, research has shown that fruit extracts can help to prolong the shelf life of food products by safeguarding them against lipid oxidation and the decline in their nutritional value. Full article
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<p>Correlation between the total phenolic content of <span class="html-italic">P. pyraster</span> and <span class="html-italic">P.</span> ×<span class="html-italic">myloslavensis</span> fruit extracts (mg GAE/g d.m.) (X-axis) and (<b>a</b>) DPPH, (<b>b</b>) the ABTS radical scavenging activity (mg Trolox/g s.m.), and (<b>c</b>) the FRAP reducing potential (mM Fe<sup>2+</sup>) (Y-axis).</p>
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20 pages, 2799 KiB  
Article
Plant-Based Ingredients Utilized as Fat Replacers and Natural Antimicrobial Agents in Beef Burgers
by Silvia Jane Lombardi, Gianfranco Pannella, Francesca Coppola, Franca Vergalito, Lucia Maiuro, Mariantonietta Succi, Elena Sorrentino, Patrizio Tremonte and Raffaele Coppola
Foods 2024, 13(20), 3229; https://doi.org/10.3390/foods13203229 - 11 Oct 2024
Viewed by 529
Abstract
The present study aimed to find solutions based on the use of plant-based ingredients that would improve the nutritional quality of meat products as well as ensure sensory and microbiological quality. Two fat replacers, lemon albedo (Citrus lemon) and carob seed [...] Read more.
The present study aimed to find solutions based on the use of plant-based ingredients that would improve the nutritional quality of meat products as well as ensure sensory and microbiological quality. Two fat replacers, lemon albedo (Citrus lemon) and carob seed gum (Ceratonia siliqua), were investigated by chemical analysis and panel testing to evaluate their effect on the nutritional and sensory quality of beef burgers. The antimicrobial activity of two plant extracts, from nettle (Urtica dioica) leaves and medlar (Eriobotrya japonica) seeds, was studied, evaluating the intensity of inhibitory action and the minimum inhibitory concentration against Pseudomonas spp. and Listeria innocua strains by plate test. In addition, the antioxidant activity of both extracts was evaluated. Based on the results, lemon albedo and medlar seed extracts were validated in a food model (beef burger) by a storage test and a challenge test. The storage test results highlight that medlar seed extract prevents the formation of thiobarbituric acid reactive substances (TBARSs) and ensures microbiological quality, inhibiting Enterobacteriaceae and Pseudomonas spp. Anti-Listeria efficacy was confirmed in situ by challenge test results. In conclusion, although fat replacers ensure nutritional and sensory quality, they do not satisfy microbiological quality. This study clearly demonstrates that the safety of low-fat burgers can only be achieved through the combination of appropriate fat replacers with well-selected natural antimicrobial extracts. Full article
(This article belongs to the Special Issue Natural Antimicrobial Agents Utilized in Food Preservation)
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Graphical abstract

Graphical abstract
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<p>Box plot showing the acceptability level (9-point hedonic scale) of sensory attributes (appareance, flavour, juiciness, residue, taste, tenderness and visible fat) in samples from batch C (conventional burgers prepared with lean minced beef and beef fat), from the batch CBG (burgers prepared with the use of carob bean gum as fat replacer), and from batch A (burgers prepared with the use of albedo flour as fat replacer). Asterix indicates significant differences (*, <span class="html-italic">p</span> &lt; 0.05; **, <span class="html-italic">p</span> &lt; 0.01; ***, <span class="html-italic">p</span> &lt; 0.001; ****, <span class="html-italic">p</span> &lt; 0.0001) in attributes between the 3 batches. Statistical test was carried out using the Kruskal–Wallis test, followed by Dunn’s post hoc test for multiple comparison.</p>
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<p>Bar plot showing the inhibition halo (cm) of medlar and nettle extracts against <span class="html-italic">L. innocua</span>, <span class="html-italic">P. fragi</span>, and <span class="html-italic">P. putida</span>. Asterix indicates significance difference (<span class="html-italic">p</span> &lt; 0.05) between extracts on the base of the statistical <span class="html-italic">t</span>-test calculated on 5 independent replicates.</p>
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<p>Model by Baranyi and Roberts [<a href="#B45-foods-13-03229" class="html-bibr">45</a>] describing the inactivation of <span class="html-italic">L. innocua</span> treated with medlar seed extract and nettle extracts used in doses of 2 times the MIC values. Symbols represent the mean of experimental data obtained by three independent experiments and lines represent the model values.</p>
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<p>Line plots showing the evolution of TMC, <span class="html-italic">Enterobacteriaceae</span> and <span class="html-italic">Pseudomonas</span> spp. during the refrigerated storage period (6 days) in hamburgers prepared conventionally (batch C), with albedo (batch A), with medlar extract (batch E) and with the combination of albedo and medlar extract (batch AE).</p>
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<p>Line plots showing the thiobarbituric acid reactive substance (TBARS) values during the refrigerated storage in burgers from batches AE (containing 10% albedo (as fat replacer) and medlar extract (0.5 mL/100 g burgers)), A (containing the albedo (10%) as fat substitute), C (containing 10% beef fat (control batch)), and E (burgers containing medlar extract (0.5 mL/100 g burgers)).</p>
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<p>Line plots showing the challenge test of <span class="html-italic">Listeria</span> spp. during the storage of burgers from batch AEL, containing 10% albedo (as fat replacer) and medlar extract (0.5 mL/100 g burgers) and inoculated with multi-strain cocktail of <span class="html-italic">Listeria</span> spp.; batch AL, containing the albedo (10%) as fat substitute and inoculated with multi-strain cocktail of <span class="html-italic">Listeria</span>; batch CL, containing 10% beef fat (control batch) and inoculated with multi-strain cocktail of <span class="html-italic">Listeria</span>; and batch EL, burgers containing medlar extract (0.5 mL/100 g burgers) and inoculated with multi-strain cocktail of <span class="html-italic">Listeria.</span></p>
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14 pages, 276 KiB  
Viewpoint
Could Snacks Based on Lupin Be a Nutritious Treat? A Point of View
by Francisco E. Carvajal Larenas, Michael J. Koziol and Mario Caviedes
Foods 2024, 13(20), 3227; https://doi.org/10.3390/foods13203227 - 11 Oct 2024
Viewed by 499
Abstract
This viewpoint article presents an opinion about snacks made with lupin species. The nutritional quality of cereal-based snacks increased significantly when lupin was added. For instance, the protein and fibre content of lupin snacks could be as high as 55.7% and 8.3% respectively, [...] Read more.
This viewpoint article presents an opinion about snacks made with lupin species. The nutritional quality of cereal-based snacks increased significantly when lupin was added. For instance, the protein and fibre content of lupin snacks could be as high as 55.7% and 8.3% respectively, soluble fibre as high as 61.2% of total fibre, and protein digestibility close to that of casein. As for sensory evaluation, some lupin snacks were ranked even better than controls. Moreover, some lupin snacks had similar or improved rheological behaviour with respect to controls. For instance, expansion indices of 11 versus 3 for controls. In summary, by adjusting formula and processing conditions, it is possible to obtain, at the same time, a healthy and tasty snack with very good machinability and rheological behaviour. This could improve the image and concept of snacks by providing an excellent opportunity for improving the diet quality of habitual consumers of snacks. This point of view also provides suggestions for improving the nutritional, rheological, and sensory evaluation of lupin snacks. Full article
17 pages, 1237 KiB  
Article
Physicochemical Properties and Nutritional Relevance of Rice Beverages Available on the Market
by Katarzyna Najman, Paulina Ponikowska, Anna Sadowska, Ewelina Hallmann, Grażyna Wasiak-Zys, Franciszek Świderski and Krzysztof Buczak
Appl. Sci. 2024, 14(19), 9150; https://doi.org/10.3390/app14199150 - 9 Oct 2024
Viewed by 493
Abstract
Recently, more consumers have reached for plant-based milk substitutes, mainly for health, dietary, and flavour reasons. This study aimed to evaluate the physicochemical and bioactive properties and sensory profile of 10 selected rice drinks available on the EU market. Assessment of the physicochemical [...] Read more.
Recently, more consumers have reached for plant-based milk substitutes, mainly for health, dietary, and flavour reasons. This study aimed to evaluate the physicochemical and bioactive properties and sensory profile of 10 selected rice drinks available on the EU market. Assessment of the physicochemical characteristics included measurement of soluble solids, pH, osmolality, foaming properties and colour parameters. Analysis of bioactive compounds involved the determination of polyphenolic compounds and antioxidant activity. Based on the manufacturer’s data, the composition and nutritional value of the above-mentioned beverages were also analysed in this study. The tested beverages were characterised by a low fat content of 0.6–1.3 g/100 mL, derived mainly from added sunflower oil, but are a good source of energy (energy value of 37–55 kcal/100 mL), derived mainly from naturally occurring carbohydrates whose content ranged from 8.0–11.0 g/100 mL. The protein content of all rice drinks was shallow, not exceeding 0.5 g/100 mL. Of the ten rice drinks analysed, in only two were vitamins B12 and D added (0.38 μg/100 mL and 0.75 μg/100 mL, respectively) and in only three was calcium (120 mg/100 mL) added. In evaluating the physicochemical characteristics, the drinks showed a pH close to neutral (mean 6.85 ± 0.01). The average soluble solids content was about 11%, which was determined by the carbohydrate content. The osmolality of the beverages averaged 324.73 ± 70.17 mOsm/kg H2O, with four beverages classifiable as hypertonic ones (osmolality > 330 mOsm/kg H2O), three as isotonic ones (with osmolality between 270–330 mOsm/kg H2O), and three as hypotonic beverages (osmolality < 270 mOsm/kg H2O). Only two rice drinks evaluated in this study showed foaming properties. The high whiteness index (81.79 ± 2.55) indicated high white colour saturation of the tested beverages. The rice drinks were characterised by a relatively diverse sensory quality regarding aroma and flavour notes. The overall sensory quality was rated highest for the beverages with the highest sugar content. The tested beverages were characterised by a low content of total polyphenols (average 1.40 ± 0.62 mg GAE/100 mL) and relatively high antioxidant activity (average 418.33 ± 59.65 µM TEAC/100 mL). Based on the research conducted and the analysis of the manufacturer’s data, it can be concluded that the rice drinks studied in this paper can be included in the daily diet, providing a rehydrating beverage that shows free radical-neutralizing properties and provides carbohydrates. At the same time, it should be highlighted that the studied drinks have a low nutritional value and cannot be recommended as milk substitutes due to low protein levels and lack of milk-specific vitamins and minerals. Full article
(This article belongs to the Section Applied Biosciences and Bioengineering)
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<p>The total polyphenol content of tested rice drinks (arithmetic mean ± standard deviation; a–i—mean values in bars marked with different letters differ significantly (<span class="html-italic">p</span> &lt; 0.05)).</p>
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<p>Antioxidant activity of the tested rice beverages (arithmetic mean ± standard deviation; a–f—mean values in bars marked with different letters differ significantly (<span class="html-italic">p</span> &lt; 0.05)).</p>
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<p>Graph of differences and similarities of the studied rice drinks. v1—colour, v2—general smell intensity, v3—sweet smell, v4—milk smell, v5—vanilla smell, v6—rice smell, v7—fat smell, v8—other (caramel) smell, v9—density, v10—viscosity, v11—sweet taste, v12—milk flavour, v13—rice flavour, v14—fat flavour, and v15—other flavour (indicated most often by evaluators as caramel), v16—“body”, v17—overall sensory quality, p1–p10—tested rice drinks, red circles indicate groups of rice beverage samples tested that are similar in terms of similar sensory attributes.</p>
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22 pages, 1520 KiB  
Review
The Role of Lactic Acid Bacteria in Meat Products, Not Just as Starter Cultures
by Kayque Ordonho Carneiro, Gabriela Zampieri Campos, João Marcos Scafuro Lima, Ramon da Silva Rocha, Manuela Vaz-Velho and Svetoslav Dimitrov Todorov
Foods 2024, 13(19), 3170; https://doi.org/10.3390/foods13193170 - 6 Oct 2024
Viewed by 657
Abstract
Lactic acid bacteria (LABs) are microorganisms of significant scientific and industrial importance and have great potential for application in meat and meat products. This comprehensive review addresses the main characteristics of LABs, their nutritional, functional, and technological benefits, and especially their importance not [...] Read more.
Lactic acid bacteria (LABs) are microorganisms of significant scientific and industrial importance and have great potential for application in meat and meat products. This comprehensive review addresses the main characteristics of LABs, their nutritional, functional, and technological benefits, and especially their importance not only as starter cultures. LABs produce several metabolites during their fermentation process, which include bioactive compounds, such as peptides with antimicrobial, antidiabetic, antihypertensive, and immunomodulatory properties. These metabolites present several benefits as health promoters but are also important from a technological point of view. For example, bacteriocins, organic acids, and other compounds are of great importance, whether from a sensory or product quality or a safety point of view. With the production of GABA, exopolysaccharides, antioxidants, and vitamins are beneficial metabolites that influence safety, technological processes, and even health-promoting consumer benefits. Despite the benefits, this review also highlights that some LABs may present virulence properties, requiring critical evaluation for using specific strains in food formulations. Overall, this review hopes to contribute to the scientific literature by increasing knowledge of the various benefits of LABs in meat and meat products. Full article
(This article belongs to the Special Issue Bio-Functional Properties of Lactic Acid Bacteria in Functional Foods)
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<p>Beneficial properties of lactic acid bacteria, from the essential driver of the fermentation processes and contributors to the technological properties of the final products to important players in the control of spoilage and pathogens and providing probiotic (and postbiotics) benefits for the consumers.</p>
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<p>General areas for effects of the bioactive peptides produced by lactic acid bacteria.</p>
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<p>Some of the areas of application of bacteriocins produced by lactic acid bacteria. From simple killing metabolites closely related to the producer’s spoilage and pathogens to potential sophisticated pharmaceuticals with application in the control of viruses, <span class="html-italic">Mycobacterium</span> spp., and cancer cells.</p>
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15 pages, 2322 KiB  
Article
Assessment of Vegetable Species for Microgreen Production in Unheated Greenhouses: Yield, Nutritional Composition, and Sensory Perception
by Pabla Rebolledo, Gilda Carrasco, Claudia Moggia, Pedro Gajardo, Gabriela Rodrigues Sant’Ana, Fernando Fuentes-Peñailillo, Miguel Urrestarazu and Eduardo Pradi Vendruscolo
Plants 2024, 13(19), 2787; https://doi.org/10.3390/plants13192787 - 4 Oct 2024
Viewed by 462
Abstract
Cultivating microgreens in central-southern Chile in unheated greenhouses offers a viable and productive alternative to growers. In 2023, two experiments were conducted in autumn and spring. These experiments involved the production of microgreens of eleven vegetable species. The tray system with the substrate [...] Read more.
Cultivating microgreens in central-southern Chile in unheated greenhouses offers a viable and productive alternative to growers. In 2023, two experiments were conducted in autumn and spring. These experiments involved the production of microgreens of eleven vegetable species. The tray system with the substrate was employed. Subsequently, agronomic, nutritional, and sensory perception variables were assessed. Despite notable fluctuations in external temperatures between these seasons, a diverse array of microgreens can be successfully cultivated, meeting local consumer preferences. Research indicates that microgreens grown under these conditions exhibit high nutritional quality, serving as a rich source of essential nutrients and bioactive compounds beneficial to human health. This nutritional value remains consistent across autumn and spring, establishing microgreens as a reliable and valuable food option. The observed acceptance and purchasing intentions among the surveyed population suggest a promising market opportunity for introducing these products regionally. Consumers appreciate microgreens’ quality and nutritional advantages, underscoring their potential. Full article
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<p>Pearson correlation network between the variables of total protein (TP), total phenols (TF), nitrates (TN), beta-carotene (BC), raw fiber (RF), iron (Fe), manganese (Mg), calcium (Ca), sodium (Na), phosphorus (P), appearance (AP), color (CL), texture (TX), and flavor (FL) of different species of microgreens evaluated during the autumn experiment. Positive correlations were highlighted in green, while negative correlations were highlighted in red; determining the line thickness followed a cut-off value of 0.7, corresponding to 70% reliability.</p>
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<p>Map of female reviewers’ purchase intention.</p>
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<p>Map of male reviewers’ purchase intention.</p>
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<p>Relative humidity values and maximum, mean, and minimum temperatures during the autumn experiment.</p>
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<p>Relative humidity values and maximum, mean, and minimum temperatures during the spring experiment.</p>
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12 pages, 1750 KiB  
Article
Utilization of Flours Derived from the Waste from the Frozen Vegetable Industry for Bakery Product Production
by Miguel A. Gallardo, María Esther Martínez-Navarro, Manuel Álvarez-Ortí and José E. Pardo
Agriculture 2024, 14(10), 1747; https://doi.org/10.3390/agriculture14101747 - 3 Oct 2024
Viewed by 481
Abstract
Currently, the agri-food industry faces a significant challenge in reducing food waste in line with circular economy principles. In this context, the frozen vegetables industry rejects products that do not meet consumers’ appearance standards, leading to a waste of vegetables that could be [...] Read more.
Currently, the agri-food industry faces a significant challenge in reducing food waste in line with circular economy principles. In this context, the frozen vegetables industry rejects products that do not meet consumers’ appearance standards, leading to a waste of vegetables that could be reincorporated into the food chain. Thus, waste generated from broccoli, cauliflower, and peas in the last selection stages of a frozen vegetable industry manufacturer were collected, dehydrated, and transformed into flour. These flours were used to replace 50% of the wheat flour in a basic bakery product, using a baked dough made only with flour and water, and analyzed from physical, nutritional, and sensory perspectives. The doughs showed slight changes in texture, with increased hardness values and reduced cohesiveness, making them more difficult to handle, as well as changes in color due to the incorporation of vegetable pigments. However, from a nutritional perspective, these products were enriched in protein, with values that reached up to 20.88% in the sample made with broccoli flour, and dietary fiber, with an increase from 0.67% obtained in the control sample to 6.00% in the sample made with pea flour and to over 8 in the samples made with broccoli and cauliflower. This was accompanied by a reduction in total carbohydrates, leading to similar energy values (around 380 kcal/100 g dm). Furthermore, the content in total phenolic compounds and antioxidant activity were increased, especially when flours from the Brassica species were used. From the sensory point of view, all the samples made with vegetable flours received positive evaluations, even higher than the control sample when smell or taste was evaluated. In this regard, the samples made with cauliflower flour stood out when the taste was evaluated, reaching values above three on a scale where the maximum value was four. All of these results demonstrate that using these wasted vegetables can be a good alternative for improving the nutritional properties of basic bakery products. Full article
(This article belongs to the Section Agricultural Product Quality and Safety)
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<p>External appearance of the flour, the dough, and the baked products made with pea, broccoli, and cauliflower waste.</p>
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<p>The evolution of color during processing, from the raw material before dehydration until the baked product. In the control sample, there was no measure for the raw material since the commercial flour was acquired in local markets directly for use. The red lines represent the red-green component (a*), while the blue lines represent the yellow-blue component (b*).</p>
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<p>The content of the total phenolic compounds in the flours (green bar) and baked products (orange bar). In both the wheat flour and the baked product obtained from it, no phenolics were detected. n.d.: not detected.</p>
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<p>Antioxidant capacity, measured as a DPPH inhibition percentage, of the wheat, pea, broccoli, and cauliflower flours, and the baked products made from them. Different letters indicate significant differences in flours or baked products (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>The average values from the sensory analysis regarding external appearance, smell, texture, and taste.</p>
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10 pages, 540 KiB  
Article
Orange Cookies with Type-4 Resistant Starch: Physical, Nutritional, and Sensorial Characteristics as Evaluated by Patients with Irritable Bowel Syndrome
by Nina G. Heredia-Sandoval, Dulce G. Machado-Duarte, Yolanda M. Preciado-Orozco, Alma R. Islas-Rubio and Ana M. Calderón de la Barca
Foods 2024, 13(19), 3144; https://doi.org/10.3390/foods13193144 - 2 Oct 2024
Viewed by 487
Abstract
A low-fermentable oligo-, di-, monosaccharide and polyol (low-FODMAP) diet for patients with irritable bowel syndrome (IBS) should include an adequate fiber source. Our aim was to formulate orange cookies using maize flour and type-4 resistant starch (RS4) from maize and to evaluate their [...] Read more.
A low-fermentable oligo-, di-, monosaccharide and polyol (low-FODMAP) diet for patients with irritable bowel syndrome (IBS) should include an adequate fiber source. Our aim was to formulate orange cookies using maize flour and type-4 resistant starch (RS4) from maize and to evaluate their properties and sensorial attributes by IBS patients. We prepared two formulations: 37.7% RS4 and 14.7% maize flour and a control with normal maize starch (MS) instead of RS4. We added orange juice and zest instead of water and evaluated their properties. The viscosity, water absorption capacity, and solubility were lower for RS4 than for MS. The width, thickness, L* and a* values of both cookies were comparable (p > 0.05), although RS4-C had a decreased b* value and higher hardness (90.6 vs. 80.1 N). The nutrient content was similar between RS4-C and MS-C, but the glycemic index of RS4-C was 63 compared to 95 of MS-C. According to IBS patients, the appearance, taste, hardness, overall quality, and perception of healthiness and nutritional value of both types of cookies were similarly high (p > 0.05). Panelists recommend the cookies. Therefore, RS4 cookies could be further investigated for their ability to improve bowel habits and re-equilibrate the microbiota of IBS patients. Full article
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<p>Digital image of RS4-containing (<b>A</b>) and MS-containing (<b>B</b>) orange cookies.</p>
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15 pages, 1983 KiB  
Proceeding Paper
Comparative Assessment of Grain Quality Characteristics and Cooking Parameters of White Rice (Oryza sativa Indica and Oryza sativa Japonica) Varieties Cultivated in Sri Lanka
by Marasingha Mudiyanselage Madhushani Thissa Marasingha, Egodage Rasanjali Jayareka Samarakoon, Banneka Mudiyanselage Kumuduni Senarathne and Haththotuwa Gamage Amal Sudaraka Samarasinghe
Eng. Proc. 2024, 67(1), 58; https://doi.org/10.3390/engproc2024067058 - 30 Sep 2024
Viewed by 186
Abstract
Traditional rice varieties (Oryza sativa L.) from Sri Lanka are less popular due to limited consumer awareness. Demand is influenced by grain quality, cooking properties, sensory attributes, and nutritional value. This study compared traditional white rice varieties with improved ones and examined [...] Read more.
Traditional rice varieties (Oryza sativa L.) from Sri Lanka are less popular due to limited consumer awareness. Demand is influenced by grain quality, cooking properties, sensory attributes, and nutritional value. This study compared traditional white rice varieties with improved ones and examined the Nipponbare Japonica variety. Milling quality varied significantly (p < 0.05) across traditional varieties, except for bran percentage. Head rice percentages ranged from 61.05 to 74.28%, and broken rice percentages ranged from 1.98 to 43.04%. Nipponbare had 48.77% of head rice and 20.78% of broken rice. Rice sizes ranged from short to long, and shapes varied from round to medium. All selected varieties had a hard gel consistency and high amylose content, except Nipponbare. Cooking parameters like elongation ratio, cooked length-to-breadth ratio, water uptake, volume expansion ratio, and cooking time showed significant differences (p < 0.05). Rathal, Suduru Samba, Suwendel, and Kahawunu are comparable to improved varieties in milling and cooking quality. The milling quality of Nipponbare is similar to improved varieties, but its cooking characteristics are intermediate compared to traditional and improved varieties. Full article
(This article belongs to the Proceedings of The 3rd International Electronic Conference on Processes)
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<p>Pericarp colors of the traditional, improved, and Japonica white rice varieties.</p>
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<p>Bulk density and true density of traditional, improved, and <span class="html-italic">Japonica</span> white rice varieties.</p>
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<p>Porosity of traditional, improved, and <span class="html-italic">Japonica</span> white rice varieties.</p>
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<p>Elongation ratio and cooked LB ratio of traditional, improved, and <span class="html-italic">Japonica</span> white rice varieties.</p>
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25 pages, 1481 KiB  
Review
From Waste to Value: Fish Protein Hydrolysates as a Technological and Functional Ingredient in Human Nutrition
by Adrián Honrado, Marta Miguel, Paula Ardila, José Antonio Beltrán and Juan B. Calanche
Foods 2024, 13(19), 3120; https://doi.org/10.3390/foods13193120 - 29 Sep 2024
Viewed by 725
Abstract
Fish provides a low-caloric content, polyunsaturated fatty acids, many essential trace elements and is also a rich source of protein, ranging from 10% to 25%. Therefore, the production of FPH (fish protein hydrolysates) is of great interest, as the resulting products exhibit a [...] Read more.
Fish provides a low-caloric content, polyunsaturated fatty acids, many essential trace elements and is also a rich source of protein, ranging from 10% to 25%. Therefore, the production of FPH (fish protein hydrolysates) is of great interest, as the resulting products exhibit a variety of important bioactive and technological properties, making them potential ingredients for new functional foods and supplements. The aim of this review was to compile and analyze information on enzymatic hydrolysates, with particular emphasis on those derived from fish by-products, as a potential ingredient in human nutrition. Their nutritional characteristics, food safety aspects, bioactive properties, technological attributes, key influencing factors, and applications in food products were evaluated. The findings revealed that these properties are influenced by several factors, such as the raw material, enzymes used, degree of hydrolysis, and the molecular weight of the peptides, which need to be considered as a whole. In conclusion, the gathered information suggests that it is possible to obtain high-value products through enzymatic hydrolysis, even when using fish by-products. However, although numerous studies focused on FPH derived from fish muscle, research on by-products remains limited. Further investigation is needed to determine whether the behavior of FPH from by-products differs from that of muscle-derived FPH. Full article
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<p>Systematic methodology followed in this research.</p>
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<p>Basic fish enzyme hydrolysates production process [<a href="#B40-foods-13-03120" class="html-bibr">40</a>].</p>
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<p>Current uses and future trends of fish protein hydrolysates.</p>
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21 pages, 1239 KiB  
Article
From Traditional to Exceptional: Impact of the Use of Dried Chicken Meat Powder on Sensory and Nutritional Quality of Tarhana
by Ramazan Ulku Cetin, Zeynep Kilci, Kivilcim Ates, Dogan Kaya and Arzu Akpinar-Bayizit
Fermentation 2024, 10(10), 501; https://doi.org/10.3390/fermentation10100501 - 29 Sep 2024
Viewed by 497
Abstract
Tarhana is a popular Turkish fermented food, made of a mixture of cereal and yoghurt, generally consumed as soup. Both lactic acid bacteria and yeasts are responsible for the fermentation. The selection and proportions of ingredients integrated along with the production process specify [...] Read more.
Tarhana is a popular Turkish fermented food, made of a mixture of cereal and yoghurt, generally consumed as soup. Both lactic acid bacteria and yeasts are responsible for the fermentation. The selection and proportions of ingredients integrated along with the production process specify the nutritional value and sensory properties of the final product. Therefore, the first objective of the present study was to process a dried “chicken meat powder (CMP)” that could be used in food formulations. The second objective was to determine the impact of CMP addition on the nutritional and sensorial properties of Uşak tarhana recipe with geographical indication. In order to fulfill these challenges dried chicken meat powder (CMP) at levels of 20, 25, 30, and 35% were included in tarhana recipes for the very first time. Within the scope of the study, organoleptic properties of tarhana soups (control and CMP added) were determined, and the formulation with 30% CMP (TCMP30) achieved the highest sensory evaluation scores. This sample was analyzed further, and its physicochemical properties were compared to a control sample strictly following the traditional Uşak tarhana recipe. In the tarhana samples, the moisture, protein, and total fat content increased with higher CMP additions, while the ash, crude cellulose, carbohydrates, energy, salt, and dietary fiber decreased significantly (p < 0.05). The calcium and sodium contents decreased, however, potassium, phosphorus, and zinc contents increased. The fatty acid composition analysis revealed that Uşak tarhana contained more saturated fatty acids than either CMP or TCMP30; adding 30% CMP reduced the total saturated fatty acids while increasing the monounsaturated fatty acids. The main fatty acids in traditional samples were linoleic, palmitic, and oleic acids, comprising 83.82% of the total fat. In TCMP30, the order changed to oleic > linoleic > palmitic with 83.89% of total fat. Over time, the total phenolic content and antioxidant capacity decreased in the control sample but increased in TCMP30. These results suggest that a formulation including 30% CMP effectively enhances the sensory, functional, and nutritional aspects in tarhana. Full article
(This article belongs to the Section Fermentation for Food and Beverages)
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<p>(<b>a</b>) Visual representations of the traditional Uşak tarhana (T), (<b>b</b>) chicken meat powder (CMP), and (<b>c</b>) Uşak tarhana samples with 30% chicken meat powder (TCMP<sub>30</sub>) produced within the scope of the study.</p>
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<p>Flow Chart of Tarhana Enriched with Dried Chicken Meat Powder.</p>
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<p>The results of the sensory evaluation of tarhana samples enriched by adding chicken meat powder at different rates <sup>+</sup> (<sup>+</sup> T: Uşak tarhana sample without the addition of chicken meat powder, TCMP<sub>20</sub>: Uşak tarhana sample with 20% chicken meat powder, TCMP<sub>25</sub>: Uşak tarhana sample with 25% chicken meat powder, TCMP<sub>30</sub>: Uşak tarhana sample with 30% chicken meat powder, TCMP<sub>35</sub>: Uşak tarhana sample with 35% chicken meat powder).</p>
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19 pages, 4242 KiB  
Article
The Impact of Ganoderma lucidum (Curtis) P. Karst. Supplementation on the Technological, Chemical, and Quality Parameters of Wheat Bread
by Paulina Łysakowska, Aldona Sobota, Anna Wirkijowska, Piotr Zarzycki and Agata Blicharz-Kania
Foods 2024, 13(19), 3101; https://doi.org/10.3390/foods13193101 - 28 Sep 2024
Viewed by 646
Abstract
This study explores the incorporation of Ganoderma lucidum (Curtis) P. Karst. (Reishi mushroom) into wheat bread to develop a functional food with enhanced nutritional value. Reishi powder was added to bread formulations at levels of 3%, 6%, 9%, and 12% to assess its [...] Read more.
This study explores the incorporation of Ganoderma lucidum (Curtis) P. Karst. (Reishi mushroom) into wheat bread to develop a functional food with enhanced nutritional value. Reishi powder was added to bread formulations at levels of 3%, 6%, 9%, and 12% to assess its effects on physicochemical, nutritional, and sensory properties. The 12% Reishi supplementation resulted in a twofold increase in total dietary fibre (from 7.21 g to 17.08 g per 100 g dry matter) and significant (p < 0.05) elevations in mineral content, particularly calcium (68%), iron (32%), and manganese (61.9%). Carbohydrate content decreased markedly by 27%, contributing to a 19.33% reduction in caloric value. Reishi addition improved bread yield and reduced baking losses, enhancing production efficiency. However, higher Reishi levels negatively impacted bread volume, possibly due to interference with gluten network formation. An increase in crumb moisture was observed, contributing to extended freshness. Sensory evaluation revealed that loaves of bread containing up to 6% Reishi were acceptable to consumers, whereas higher levels detrimentally affected flavour and aroma. Therefore, Reishi-enriched bread, particularly with 6% supplementation, presents a promising functional alternative to conventional wheat bread, optimising nutritional benefits while maintaining consumer acceptability. Full article
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<p>Porosity of fresh crumb of wheat bread (CON) and bread enriched with 3, 6, 9, and 12% Reishi (BR).</p>
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<p>Samples of wheat bread (CON) and bread (BR) enriched with 3, 6, 9, and 12% Reishi.</p>
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<p>Results of the sensory evaluation of the bread. CON—control sample (100% wheat bread); BR—bread with 3, 6, 9, and 12% addition of Reishi.</p>
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