[go: up one dir, main page]

 
 
Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Article Types

Countries / Regions

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Search Results (8,513)

Search Parameters:
Keywords = environmental stress

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
21 pages, 2219 KiB  
Review
Internal Disorders of Mango Fruit and Their Management—Physiology, Biochemistry, and Role of Mineral Nutrients
by Muhammad Asad Ullah, Amit Khanal, Priya Joyce, Neil White, Andrew Macnish and Daryl Joyce
Plants 2024, 13(18), 2596; https://doi.org/10.3390/plants13182596 (registering DOI) - 17 Sep 2024
Abstract
Mango (Mangifera indica L.) is a popular fruit grown in tropical and subtropical regions. Mango has a distinctive aroma, flavour, and nutritional properties. Annual global mango production is >50 million tonnes. Major producers of mango include India, Bangladesh, China, Mexico, Pakistan, Indonesia, [...] Read more.
Mango (Mangifera indica L.) is a popular fruit grown in tropical and subtropical regions. Mango has a distinctive aroma, flavour, and nutritional properties. Annual global mango production is >50 million tonnes. Major producers of mango include India, Bangladesh, China, Mexico, Pakistan, Indonesia, Brazil, Thailand, and the Philippines, and it is shipped worldwide. Harvested mango fruit are highly perishable, with a short shelf life. Physiological disorders are among the major factors limiting their postharvest quality and shelf life, including when fruit need phytosanitary treatments, such as hot water treatment, vapour heat treatment, and irradiation. This review focuses on problematic physiological disorders of mango flesh, including physiology and biochemistry. It considers factors contributing to the development and/or exacerbation of internal disorders. Improved production practices, including pruning, nutrient application, and irrigation, along with monitoring and managing environmental conditions (viz., temperature, humidity, and vapour pressure deficit), can potentially maintain fruit robustness to better tolerate otherwise stressful postharvest operations. As demand for mangoes on international markets is compromised by internal quality, robust fruit is crucial to maintaining existing and gaining new domestic and export consumer markets. Considering mango quality, a dynamic system, a more holistic approach encompassing pre-, at-, and post-harvest conditions as a continuum is needed to determine fruit predisposition and subsequent management of internal disorders. Full article
(This article belongs to the Section Horticultural Science and Ornamental Plants)
Show Figures

Figure 1

Figure 1
<p>Characteristic morphological features of the mango.</p>
Full article ">Figure 2
<p>Internal disorders in cv. ‘B74’ mango: (<b>a</b>) Soft nose [<a href="#B40-plants-13-02596" class="html-bibr">40</a>], (<b>b</b>) stem-end cavity [<a href="#B41-plants-13-02596" class="html-bibr">41</a>], (<b>c</b>) jelly seed [<a href="#B40-plants-13-02596" class="html-bibr">40</a>], (<b>d</b>) internal flesh browning [<a href="#B41-plants-13-02596" class="html-bibr">41</a>], and (<b>e</b>) spongy tissue in cv. ‘Alphonso’ [<a href="#B9-plants-13-02596" class="html-bibr">9</a>].</p>
Full article ">Figure 3
<p>Processes and their influences on calcium uptake, translocation, and partitioning in fruit trees. ‘*’ indicate key factors involved in Ca calcium partitioning at branch level.</p>
Full article ">Figure 4
<p>Major considerations at preharvest, harvest, and postharvest stages across the ‘genotype × environment × management’ spectrum to produce robust mango fruit.</p>
Full article ">
14 pages, 5733 KiB  
Article
N-Acetylcysteine Mitigates Oxidative Stress Induced by Transplanting Lettuce Seedlings into a DFT Hydroponic System
by Masaru Sakamoto and Takahiro Suzuki
Agronomy 2024, 14(9), 2112; https://doi.org/10.3390/agronomy14092112 (registering DOI) - 17 Sep 2024
Abstract
Oxidative stress results from an imbalance between the production and accumulation of reactive oxygen species (ROS), which can impede plant growth under various environmental stresses. While waterlogging is a well-known inducer of oxidative stress, the effects of oxidative stress on plant roots grown [...] Read more.
Oxidative stress results from an imbalance between the production and accumulation of reactive oxygen species (ROS), which can impede plant growth under various environmental stresses. While waterlogging is a well-known inducer of oxidative stress, the effects of oxidative stress on plant roots grown using the deep flow technique (DFT) hydroponic system remain poorly understood. In this study, we demonstrate that N-acetylcysteine (NAC) enhances the growth of lettuce seedlings transplanted into a DFT system. NAC application significantly improved both shoot and root growth, with the most pronounced effects observed at a concentration of 0.3 mM. Moreover, NAC mitigated the accumulation of hydrogen peroxide in roots following transplantation. It also reduced a temporary increase in lipid peroxidation and total phenolic content in both roots and shoots. These results suggest that NAC functions as an antioxidant, alleviating oxidative stress by scavenging hydrogen peroxide in the roots. Importantly, NAC’s protective effects may extend to other hydroponically grown crops, offering broader potential for reducing oxidative stress across various cultivation systems. Full article
(This article belongs to the Section Plant-Crop Biology and Biochemistry)
Show Figures

Figure 1

Figure 1
<p>The effect of different NAC concentrations on the shoot morphology of DFT-transplanted lettuces was assessed 7 days after treatments. NAC was applied to 14-day-old plants via the nutrient solution at concentrations of 0, 0.03, 0.1, 0.3, 1.0, and 3.0 mM. Scale bar is 10 cm.</p>
Full article ">Figure 2
<p>The effect of different NAC concentrations on the shoot and root growth of DFT-transplanted lettuces was assessed at 7 days after treatments. NAC was applied to 14-day-old plants via the nutrient solution at concentrations of 0, 0.03, 0.1, 0.3, 1.0, and 3.0 mM. (<b>A</b>) Shoot fresh weight. (<b>B</b>) Shoot dry weight. (<b>C</b>) Shoot water content. (<b>D</b>) Root fresh weight. (<b>E</b>) Root dry weight. (<b>F</b>) Root water content. Vertical bars represent ± SE (<span class="html-italic">n</span> = 6). Different letters indicate a significant difference per Tukey’s multiple comparison test (<span class="html-italic">p</span> &lt; 0.05).</p>
Full article ">Figure 3
<p>The effect of various nutrient concentrations on the growth-promoting effects of NAC in DFT-transplanted lettuces was examined at 7 days after treatments. NAC (0.3 mM) and different nutrient concentrations of the Otsuka House A-recipe were applied to 14-day-old plants. (<b>A</b>) Shoot fresh weight. (<b>B</b>) Shoot dry weight. (<b>C</b>) Shoot water content. (<b>D</b>) Root fresh weight. (<b>E</b>) Root dry weight. (<b>F</b>) Root water content. Vertical bars represent ± SE (<span class="html-italic">n</span> = 6). Different letters indicate a significant difference per Tukey’s multiple comparison test (<span class="html-italic">p</span> &lt; 0.05).</p>
Full article ">Figure 4
<p>The effect of NAC on hydrogen peroxide production in DFT-transplanted lettuce roots. Hydrogen peroxide content in plant roots at 3 days after treatments was quantified spectrophotometrically in roots (<b>A</b>). Additionally, hydrogen peroxide was visualized throughout the roots (<b>B</b>) and root tips using DAB staining under a microscope (<b>C</b>). Vertical bars in (<b>A</b>) represent ± SE (<span class="html-italic">n</span> = 4). An asterisk indicates that values between treatments are significantly different according to the independent <span class="html-italic">t</span>-test, with <span class="html-italic">p</span> &lt; 0.01. Scale bars are 2 cm in (<b>B</b>), and 100 μm in (<b>C</b>).</p>
Full article ">Figure 5
<p>The effect of NAC on lipid peroxidation in DFT-transplanted lettuce roots. Root lipid peroxidation was visualized with Schiff’s reagent. Scale bars are 100 μm.</p>
Full article ">Figure 6
<p>The effect of NAC on lipid peroxidation in DFT-transplanted lettuce roots (<b>A</b>) and shoots (<b>B</b>) over 7 days after treatments. Lipid peroxidation was monitored by measuring the malondialdehyde (MDA) concentration. Vertical bars represent ± SE (<span class="html-italic">n</span> = 4). Asterisks indicate significant differences within each day between treatments according to the independent <span class="html-italic">t</span>-test, with <span class="html-italic">p</span> &lt; 0.05. The absence of a symbol indicates no significant differences.</p>
Full article ">Figure 7
<p>The effect of NAC on total phenolic content in DFT-transplanted lettuce roots (<b>A</b>) and shoots (<b>B</b>) over 7 days after treatments. Vertical bars represent ± SE (<span class="html-italic">n</span> = 4). Asterisks indicate significant differences within each day between treatments according to the independent <span class="html-italic">t</span>-test, with <span class="html-italic">p</span> &lt; 0.05. The absence of a symbol indicates no significant differences.</p>
Full article ">Figure 8
<p>The effect of NAC on DPPH radical scavenging capacity in DFT-transplanted lettuce roots (<b>A</b>) and shoots (<b>B</b>) over 7 days after treatments. Vertical bars represent ± SE (<span class="html-italic">n</span> = 4). Asterisks indicate significant differences within each day between treatments according to the independent <span class="html-italic">t</span>-test, with <span class="html-italic">p</span> &lt; 0.05. The absence of a symbol indicates no significant differences.</p>
Full article ">Figure 9
<p>The effect of NAC on nitrate content in DFT-transplanted lettuce roots (<b>A</b>) and shoots (<b>B</b>) over 7 days after treatments. Vertical bars represent ± SE (<span class="html-italic">n</span> = 4). Asterisks indicate significant differences within each day between treatments according to the independent <span class="html-italic">t</span>-test, with <span class="html-italic">p</span> &lt; 0.05. The absence of a symbol indicates no significant differences.</p>
Full article ">
19 pages, 7026 KiB  
Article
Bond-Slip Constitutive Relationship between Steel Rebar and Concrete Synthesized from Solid Waste Coal Gasification Slag
by Huawei Li, Haozhe Chen, Qingke Nie, Junchao Yu, Liang Zhang and Qingjun Wang
Buildings 2024, 14(9), 2931; https://doi.org/10.3390/buildings14092931 - 16 Sep 2024
Viewed by 218
Abstract
Bond performance served as a crucial foundation for the collaboration between concrete and steel rebar. This study investigated the bond performance between coal gasification slag (CGS) concrete, an environmentally friendly construction material, and steel rebar. The effects of fine aggregate type, steel rebar [...] Read more.
Bond performance served as a crucial foundation for the collaboration between concrete and steel rebar. This study investigated the bond performance between coal gasification slag (CGS) concrete, an environmentally friendly construction material, and steel rebar. The effects of fine aggregate type, steel rebar diameter, and anchorage length on bond performance were examined through bond-slip tests conducted on 16 groups of reinforced concrete specimens with different parameters. By utilizing experimental data, a formula for the bond strength between steel rebar and CGS concrete was derived. Additionally, the BPE bond-slip constitutive model was modified by introducing a correction factor (k) to account for relative protective layer thickness. Findings indicated that substituting 25% of manufactured sand with coal gasification slag did not cause significant adverse effects on concrete strength or bond stress between concrete and steel rebar. The effect of steel rebar diameter on the ultimate bond stress was not obvious, whereas when the steel rebar diameter was fixed; the increase in anchorage length led to uneven distribution of bond stress and eventually reduced the ultimate bond stress. The modified bond-slip constitutive model agreed well with the experimental values and was able to more accurately reflect the bond-slip performance between CGS concrete and steel rebar. This study provided a theoretical basis for the conversion of CGS into a resource and for the application of CGS concrete. Full article
(This article belongs to the Special Issue Research and Utilization of Solid Waste and Construction Waste)
Show Figures

Figure 1

Figure 1
<p>Particle size distribution of cement.</p>
Full article ">Figure 2
<p>Fine aggregate: (<b>a</b>) CGS and (<b>b</b>) manufactured sand.</p>
Full article ">Figure 3
<p>Particle grading curves of fine aggregate.</p>
Full article ">Figure 4
<p>Dimensional design of specimens (mm).</p>
Full article ">Figure 5
<p><span class="html-italic">τ</span>-<span class="html-italic">s</span> curves of specimens: (<b>a</b>) PR14 group; (<b>b</b>) R14 group; (<b>c</b>) R18 group and (<b>d</b>) R22 group.</p>
Full article ">Figure 6
<p>Steel rebar pull-out damage of R22-5d.</p>
Full article ">Figure 7
<p>Concrete splitting damage of R18-10d.</p>
Full article ">Figure 8
<p>Typical <span class="html-italic">τ</span>-<span class="html-italic">s</span> curve: R22-7d and R22-10d.</p>
Full article ">Figure 9
<p><span class="html-italic">τ</span>-<span class="html-italic">s</span> curves for specimens of different concrete types: (<b>a</b>) comparison of PR14 and R14 at anchorage lengths of 3d and 5d and (<b>b</b>) comparison of PR14 and R14 at anchorage lengths of 7d and 10d.</p>
Full article ">Figure 10
<p>Effect of steel rebar diameter: (<b>a</b>) <span class="html-italic">τ</span>-<span class="html-italic">s</span> curves for different steel rebar diameters and (<b>b</b>) correspondence between <span class="html-italic">τ<sub>u</sub></span> and <span class="html-italic">d</span>.</p>
Full article ">Figure 11
<p>Modified BPE constitutive model.</p>
Full article ">Figure 12
<p>Fitting of the shape parameter <span class="html-italic">α</span>.</p>
Full article ">Figure 13
<p>Validation of the <span class="html-italic">τ</span>-<span class="html-italic">s</span> constitutive model.</p>
Full article ">Figure 13 Cont.
<p>Validation of the <span class="html-italic">τ</span>-<span class="html-italic">s</span> constitutive model.</p>
Full article ">
26 pages, 37606 KiB  
Review
Nanomaterials for Modified Asphalt and Their Effects on Viscosity Characteristics: A Comprehensive Review
by Hualong Huang, Yongqiang Wang, Xuan Wu, Jiandong Zhang and Xiaohan Huang
Nanomaterials 2024, 14(18), 1503; https://doi.org/10.3390/nano14181503 - 16 Sep 2024
Viewed by 253
Abstract
The application of nanomaterials as modifiers in the field of asphalt is increasingly widespread, and this paper aims to systematically review research on the impact of nanomaterials on asphalt viscosity. The results find that nanomaterials tend to increase asphalt’s viscosity, enhancing its resistance [...] Read more.
The application of nanomaterials as modifiers in the field of asphalt is increasingly widespread, and this paper aims to systematically review research on the impact of nanomaterials on asphalt viscosity. The results find that nanomaterials tend to increase asphalt’s viscosity, enhancing its resistance to high-temperature rutting and low-temperature cracking. Zero-dimension nanomaterials firmly adhere to the asphalt surface, augmenting non-bonding interactions through van der Waals forces and engaging in chemical reactions to form a spatial network structure. One-dimensional nanomaterials interact with non-polar asphalt molecules, forming bonds between tube walls, thereby enhancing adhesion, stability, and resistance to cyclic loading. Meanwhile, these bundled materials act as reinforcement to transmit stress, preventing or delaying crack propagation. Two-dimensional nanomaterials, such as graphene and graphene oxide, participate in chemical interactions, forming hydrogen bonds and aromatic deposits with asphalt molecules, affecting asphalt’s surface roughness and aggregate movement, which exhibit strong adsorption capacity and increase the viscosity of asphalt. Polymers reduce thermal movement and compact asphalt structures, absorbing light components and promoting the formation of a cross-linked network, thus enhancing high-temperature deformation resistance. However, challenges such as poor compatibility and dispersion, high production costs, and environmental and health concerns currently hinder the widespread application of nanomaterial-modified asphalt. Consequently, addressing these issues through comprehensive economic and ecological evaluations is crucial before large-scale practical implementation. Full article
Show Figures

Figure 1

Figure 1
<p>Classification of nano-modified materials.</p>
Full article ">Figure 2
<p>Shape and structure of NZ: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Refs. [<a href="#B6-nanomaterials-14-01503" class="html-bibr">6</a>,<a href="#B36-nanomaterials-14-01503" class="html-bibr">36</a>]. Copyrights 2023 and 2024 MDPI.</p>
Full article ">Figure 3
<p>Shape and structure of NS: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Refs. [<a href="#B41-nanomaterials-14-01503" class="html-bibr">41</a>,<a href="#B42-nanomaterials-14-01503" class="html-bibr">42</a>]. Copyrights 2024 MDPI and 2023 Elsevier.</p>
Full article ">Figure 4
<p>Shape and structure of NT: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Refs. [<a href="#B41-nanomaterials-14-01503" class="html-bibr">41</a>,<a href="#B48-nanomaterials-14-01503" class="html-bibr">48</a>]. Copyrights 2024 and 2023 MDPI.</p>
Full article ">Figure 5
<p>Shape and structure of NA: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Refs. [<a href="#B41-nanomaterials-14-01503" class="html-bibr">41</a>,<a href="#B51-nanomaterials-14-01503" class="html-bibr">51</a>]. Copyrights 2024 Elsevier and MDPI.</p>
Full article ">Figure 6
<p>Shape and structure of NCa: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Ref. [<a href="#B42-nanomaterials-14-01503" class="html-bibr">42</a>]. Copyright 2023 Elsevier.</p>
Full article ">Figure 7
<p>Shape and structure of NFe: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Ref. [<a href="#B57-nanomaterials-14-01503" class="html-bibr">57</a>]. Copyright 2017 Elsevier.</p>
Full article ">Figure 8
<p>Shape and structure of CNT: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Ref. [<a href="#B63-nanomaterials-14-01503" class="html-bibr">63</a>]. Copyright 2021 Elsevier.</p>
Full article ">Figure 9
<p>Schematic diagram of CNT distribution in asphalt. Adapted with permission from Ref. [<a href="#B64-nanomaterials-14-01503" class="html-bibr">64</a>]. Copyright 2020 Elsevier.</p>
Full article ">Figure 10
<p>Shape and structure of nanofibers: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Refs. [<a href="#B66-nanomaterials-14-01503" class="html-bibr">66</a>,<a href="#B67-nanomaterials-14-01503" class="html-bibr">67</a>]. Copyrights Springer Nature and 2021 Elsevier.</p>
Full article ">Figure 11
<p>Shape and structure of graphene: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Refs. [<a href="#B71-nanomaterials-14-01503" class="html-bibr">71</a>,<a href="#B72-nanomaterials-14-01503" class="html-bibr">72</a>]. Copyrights 2021 and 2022 Elsevier.</p>
Full article ">Figure 12
<p>Mechanism of graphene-modified asphalt: (<b>a</b>) interface π–π interaction; (<b>b</b>) filling and barrier structure. Adapted with permission from Refs. [<a href="#B77-nanomaterials-14-01503" class="html-bibr">77</a>,<a href="#B78-nanomaterials-14-01503" class="html-bibr">78</a>]. Copyrights 2021 and 2018 Elsevier.</p>
Full article ">Figure 13
<p>Shape and structure of GO: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Refs. [<a href="#B9-nanomaterials-14-01503" class="html-bibr">9</a>,<a href="#B83-nanomaterials-14-01503" class="html-bibr">83</a>]. Copyrights 2022 Hindawi and 2017 Springer.</p>
Full article ">Figure 14
<p>Mechanism of GO-modified asphalt: (<b>a</b>) adsorption; (<b>b</b>) hydrogen bonding interaction. Adapted with permission from Ref. [<a href="#B82-nanomaterials-14-01503" class="html-bibr">82</a>]. Copyright Elsevier.</p>
Full article ">Figure 15
<p>Shape and structure of NC: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Ref. [<a href="#B90-nanomaterials-14-01503" class="html-bibr">90</a>]. Copyrights 2023 MDPI.</p>
Full article ">Figure 16
<p>Shape and structure of SBS: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Refs. [<a href="#B94-nanomaterials-14-01503" class="html-bibr">94</a>,<a href="#B95-nanomaterials-14-01503" class="html-bibr">95</a>,<a href="#B96-nanomaterials-14-01503" class="html-bibr">96</a>]. Copyrights 2020 Elsevier, 2023 Walter de Gruyter, and 2021 John Wiley and Sons Inc.</p>
Full article ">Figure 17
<p>Shape and structure of SBR: (<b>a</b>) macroscopic scale; (<b>b</b>) microscale; (<b>c</b>) molecular scale. Adapted with permission from Refs. [<a href="#B95-nanomaterials-14-01503" class="html-bibr">95</a>,<a href="#B101-nanomaterials-14-01503" class="html-bibr">101</a>,<a href="#B102-nanomaterials-14-01503" class="html-bibr">102</a>]. Copyrights 2023 Walter de Gruyter and 2024 MDPI.</p>
Full article ">Figure 18
<p>Cross-linked network between SBR and asphalt molecules. Adapted with permission from Ref. [<a href="#B97-nanomaterials-14-01503" class="html-bibr">97</a>]. Copyrights 2024 Elsevier.</p>
Full article ">Figure 19
<p>Viscosity temperature curves of matrix asphalt and NT/NCa-modified asphalt. Adapted with permission from Ref. [<a href="#B103-nanomaterials-14-01503" class="html-bibr">103</a>]. Copyrights 2021 Hindawi.</p>
Full article ">Figure 20
<p>Physical moduli of asphalt and NZ/SBS/asphalt. Adapted with permission from Ref. [<a href="#B94-nanomaterials-14-01503" class="html-bibr">94</a>]. Copyrights 2020 Elsevier.</p>
Full article ">Figure 21
<p>Viscosity–temperature relationship curves of three types of asphalt. Adapted with permission from Ref. [<a href="#B111-nanomaterials-14-01503" class="html-bibr">111</a>]. Copyrights 2022 MDPI.</p>
Full article ">Figure 22
<p>Interface microstructure of GO/SBS-modified asphalt. Adapted with permission from Ref. [<a href="#B114-nanomaterials-14-01503" class="html-bibr">114</a>]. Copyrights 2023 Springer Nature.</p>
Full article ">Figure 23
<p>Viscosity of modified asphalt with different modifiers. Adapted with permission from Ref. [<a href="#B119-nanomaterials-14-01503" class="html-bibr">119</a>]. Copyrights 2018 Hindawi.</p>
Full article ">
19 pages, 2944 KiB  
Article
Dynamic Membrane Lipid Changes in Physcomitrium patens Reveal Developmental and Environmental Adaptations
by Deepshila Gautam, Jyoti R. Behera, Suhas Shinde, Shivakumar D. Pattada, Mary Roth, Libin Yao, Ruth Welti and Aruna Kilaru
Biology 2024, 13(9), 726; https://doi.org/10.3390/biology13090726 - 16 Sep 2024
Viewed by 475
Abstract
Membrane lipid composition is critical for an organism’s growth, adaptation, and functionality. Mosses, as early non-vascular land colonizers, show significant adaptations and changes, but their dynamic membrane lipid alterations remain unexplored. Here, we investigated the temporal changes in membrane lipid composition of the [...] Read more.
Membrane lipid composition is critical for an organism’s growth, adaptation, and functionality. Mosses, as early non-vascular land colonizers, show significant adaptations and changes, but their dynamic membrane lipid alterations remain unexplored. Here, we investigated the temporal changes in membrane lipid composition of the moss Physcomitrium patens during five developmental stages and analyzed the acyl content and composition of the lipids. We observed a gradual decrease in total lipid content from the filamentous protonema stage to the reproductive sporophytes. Notably, we found significant levels of very long-chain polyunsaturated fatty acids, particularly arachidonic acid (C20:4), which are not reported in vascular plants and may aid mosses in cold and abiotic stress adaptation. During vegetative stages, we noted high levels of galactolipids, especially monogalactosyldiacylglycerol, associated with chloroplast biogenesis. In contrast, sporophytes displayed reduced galactolipids and elevated phosphatidylcholine and phosphatidic acid, which are linked to membrane integrity and environmental stress protection. Additionally, we observed a gradual decline in the average double bond index across all lipid classes from the protonema stage to the gametophyte stage. Overall, our findings highlight the dynamic nature of membrane lipid composition during moss development, which might contribute to its adaptation to diverse growth conditions, reproductive processes, and environmental challenges. Full article
(This article belongs to the Special Issue Lipid Metabolism in Plant Growth and Development)
Show Figures

Figure 1

Figure 1
<p>Fatty acid profile of different developmental stages in <span class="html-italic">P. patens</span>. (<b>A</b>) Visual representation of five developmental stages of the moss; protonema (PN), early gametophore (EG), mid-gametophore (MG), late gametophore (LG) and sporophyte (SP); (<b>B</b>) Total lipid content of each developmental stage in mg/g fresh weight (FW) of tissue; (<b>C</b>) Distribution of fatty acids (FAs) in the sporophyte; (<b>D</b>) Major fatty acid (top) and minor fatty acid levels in <span class="html-italic">P. patens</span> during developmental stages.</p>
Full article ">Figure 2
<p>Major and minor lipid classes of the moss. Lipid content in various lipid classes at different developmental stages; protonema (PN), early gametophore (EG), mid-gametophore (MG), late gametophore (LG) and sporophyte (SP).</p>
Full article ">Figure 3
<p>Distribution of lipid classes. The percentage of each lipid class is shown for various developmental stages of the moss (protonema (PN), early gametophore (EG), mid-gametophore (MG), late gametophore (LG) and sporophyte (SP)), Selaginella (Sm), Arabidopsis (At) seedlings and seeds [<a href="#B27-biology-13-00726" class="html-bibr">27</a>], and mouse [<a href="#B28-biology-13-00726" class="html-bibr">28</a>]. The graphs indicate percentage of the total lipid weight for each lipid class.</p>
Full article ">Figure 4
<p>The acyl composition of galactolipids. Acyl composition of MGDG and DGDG in various developmental stages of the moss (protonema (PN), early gametophore (EG), mid-gametophore (MG), late gametophore (LG) and sporophyte (SP) and comparison with Selaginella (Sm) and Arabidopsis (At) 8-day seedlings [<a href="#B27-biology-13-00726" class="html-bibr">27</a>].</p>
Full article ">Figure 5
<p>Acyl composition of PC. The acyl composition of various developmental stages (various developmental stages of the moss (protonema (PN), early gametophore (EG), mid-gametophore (MG), late gametophore (LG) and sporophyte (SP)) of the moss, Selaginella (Sm) [<a href="#B27-biology-13-00726" class="html-bibr">27</a>].</p>
Full article ">Figure 6
<p>Acyl composition of PE. The acyl composition of PE in developmental stages of the moss (protonema (PN), early gametophore (EG), mid-gametophore (MG), late gametophore (LG) and sporophyte (SP) and comparison with Selaginella (Sm).</p>
Full article ">Figure 7
<p>Double bond index (DBI) of major and minor lipid classes during <span class="html-italic">P. patens</span> development. The values are shown as heat map with red being the highest, cyan showing the lowest, and yellow representing the mid-range value. The highest DBI values in each growth stages are shown as highlighted red color text. Monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidic acid (PA), lysophosphatidylglycerol (LysoPG), lysophosphatidylcholine (LysoPC), and lysophosphatidylethanolamine (LysoPE).</p>
Full article ">
22 pages, 15397 KiB  
Article
The F-Box Protein TaFBA1 Positively Regulates Drought Resistance and Yield Traits in Wheat
by Qinxue Li, Xiaoyu Zhao, Jiajie Wu, Huixia Shou and Wei Wang
Plants 2024, 13(18), 2588; https://doi.org/10.3390/plants13182588 - 16 Sep 2024
Viewed by 262
Abstract
Environmental stresses, including drought stress, seriously threaten food security. Previous studies reported that wheat F-box protein, TaFBA1, responds to abiotic stresses in tobacco. Here, we generated transgenic wheat with enhanced (overexpression, OE) or suppressed (RNA interference, RNAi) expression of TaFBA1. The TaFBA1 [...] Read more.
Environmental stresses, including drought stress, seriously threaten food security. Previous studies reported that wheat F-box protein, TaFBA1, responds to abiotic stresses in tobacco. Here, we generated transgenic wheat with enhanced (overexpression, OE) or suppressed (RNA interference, RNAi) expression of TaFBA1. The TaFBA1-OE seedlings showed enhanced drought tolerance, as measured by survival rate and fresh weight under severe drought stress, whereas the RNAi plants showed the opposite phenotype. Furthermore, the OE plants had stronger antioxidant capacity compared to WT and RNAi plants and maintained stomatal opening, which resulted in higher water loss under drought stress. However, stronger water absorption capacity in OE roots contributed to higher relative water contents in leaves under drought stress. Moreover, the postponed stomatal closure in OE lines helped to maintain photosynthesis machinery to produce more photoassimilate and ultimately larger seed size. Transcriptomic analyses conducted on WT and OE plants showed that genes involved in antioxidant, fatty acid and lipid metabolism and cellulose synthesis were significantly induced by drought stress in the leaves of OE lines. Together, our studies determined that the F-box protein TaFBA1 modulated drought tolerance and affected yield in wheat and the TaFBA1 gene could provide a desirable target for further breeding of wheat. Full article
(This article belongs to the Special Issue Research on Plant Genomics and Breeding 2023)
Show Figures

Figure 1

Figure 1
<p>Molecular identification of TaFBA1 transgenic lines. Schematic diagrams of constructs used for (<b>A</b>) TaFBA1 overexpression (FO) and (<b>B</b>) RNAi-mediated knockdown (FR). The expression levels of TaFBA1 in (<b>C</b>) FO and (<b>D</b>) FR lines were assessed using qRT-PCR. The data represent the mean ± SD of three biological replicates. ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 2
<p>Analysis of drought tolerance in WT and <span class="html-italic">TaFBA1</span> transgenic wheat lines at the heading stage. (<b>A</b>) Phenotype and (<b>B</b>) magnifying local picture of 32-day-old wheat exposed to 20% PEG6000 for 2 and 3 weeks. Plant height after drought stress for (<b>C</b>) 2 weeks and (<b>D</b>) 3 weeks, respectively. Leaf wilting rate (%) after drought stress for (<b>E</b>) 2 weeks and (<b>F</b>) 3 weeks. The data represent the mean ± SD of three biological replicates. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 3
<p>Effects of <span class="html-italic">TaFBA1</span> overexpression on wheat grain traits under normal and drought stress conditions. (<b>A</b>) Grain length and width phenotype of 20 mature wheat grains harvested under normal and 20% PEG6000 treatment conditions. (<b>B</b>) Spike size and hundred-grain volume of mature wheat grains. (<b>C</b>) Average grain length. (<b>D</b>) Average grain width. (<b>E</b>) Grain number per spike. (<b>F</b>) Grain weight per spike. (<b>G</b>) Hundred-grain weight. The data represent the mean ± SD of three biological replicates. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 4
<p>Photosynthetic capacity of WT and transgenic wheat lines under drought stress. WT, <span class="html-italic">TaFBA1</span>-OE and <span class="html-italic">TaFBA1</span>-RNAi wheat lines were grown under normal condition and under dehydration with 20% PEG6000 for 2 weeks before determination of the (<b>A</b>) Net photosynthetic rate (Pn), the (<b>B</b>) transpiration rate (E), the (<b>C</b>) stomatal conductance (Gs), the (<b>D</b>) actual PSII efficiency (ΦPSII), the (<b>E</b>) maximum photochemical efficiency of PSII (Fv/Fm), the (F) intercellular CO<sub>2</sub> concentration (Ci), the contents of (<b>G</b>) chlorophyll a and (<b>H</b>) chlorophyll b. The data represent the mean ± SD of three biological replicates. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 5
<p>Reactive Oxygen Species (ROS) accumulation and cell membrane oxidative damage in WT and transgenic lines under drought stress. Two-week-old seedlings of WT, <span class="html-italic">TaFBA1</span>-OE and <span class="html-italic">TaFBA1</span>-RNAi wheat exposed to PEG6000 as dehydration stress for about 5 days. Leaves were stained with (<b>A</b>) NBT and (<b>B</b>) DAB staining for detecting O<sup>2−</sup> and H<sub>2</sub>O<sub>2</sub> levels. Quantification of (<b>B</b>) O<sup>2−</sup> and (<b>C</b>) H<sub>2</sub>O<sub>2</sub> levels in leaves as above. The (<b>D</b>) MDA content and (<b>E</b>) relative electrolyte leakage were determined in the lines grown in normal and dehydration conditions. Protein carbonylation, as another measure of oxidative stress is presented in <a href="#app1-plants-13-02588" class="html-app">Figure S3</a>. The data represent the mean ± SD of three biological replicates. ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 6
<p>Antioxidative abilities of WT and transgenic wheat lines under normal and drought stress. Two-week-old seedlings of WT, <span class="html-italic">TaFBA1</span>-OE and <span class="html-italic">TaFBA1</span>-RNAi wheat exposed to PEG6000 as dehydration stress for about 5 days. Leaves were sampled for the determination of antioxidase enzyme activities. SOD (<b>A</b>), CAT, (<b>B</b>) APX (<b>C</b>), POD (<b>D</b>), GPx (<b>E</b>), GR (<b>F</b>), MDAR (<b>G</b>), and DHAR (<b>H</b>) activities in wheat grown under normal and drought stress conditions. The data represent the mean ± SD of three biological replicates. * <span class="html-italic">p</span> &lt;0.05; ** <span class="html-italic">p</span>&lt; 0.01.</p>
Full article ">Figure 7
<p>Relative expression of antioxidant-related and stress-responsive genes in WT and transgenic wheat lines. Relative expression of antioxidant-related genes, namely <span class="html-italic">TaCu/Zn-SOD</span> (<b>A</b>), <span class="html-italic">TaMn-SOD</span> (<b>B</b>), <span class="html-italic">TaFe-SOD</span> (<b>C</b>), <span class="html-italic">TaAPX</span> (<b>D</b>), <span class="html-italic">TaPOD</span> (<b>E</b>), <span class="html-italic">TaCAT</span> (<b>F</b>), and <span class="html-italic">TaGPX</span> (<b>G</b>), <span class="html-italic">TaGR</span> (<b>H</b>), <span class="html-italic">TaDHAR</span> (<b>I</b>), and <span class="html-italic">TaMDAR</span> (<b>J</b>) and stress-responsive genes, namely <span class="html-italic">TaLEA7</span> (<b>K</b>), <span class="html-italic">TaRD29B</span> (<b>L</b>), <span class="html-italic">TaDREB6</span> (<b>M</b>), <span class="html-italic">TaFER-5B</span> (<b>N</b>), <span class="html-italic">TaSAPK2</span> (<b>O</b>), and <span class="html-italic">TaP5CS</span> (<b>P</b>), in the flag leaves of WT and transgenic wheat under drought stress. The data represent the mean ± SD of three biological replicates. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 8
<p>Stomatal aperture on the surface of WT and transgenic wheat lines leaves. (<b>A</b>) Images of stoma with different aperture on the leaves of WT and transgenic wheat after PEG treatment obtained using a fluorescence microscope. (<b>B</b>) Percentage of stoma of different aperture. (<b>C</b>) Water loss rate of detached leaves of WT and transgenic wheat lines. (<b>D</b>) Relative water content (RWC) in leaves of all lines grown under normal and drought stress conditions. The data represent the mean ± SD of three biological replicates. ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 9
<p>Response of shoot and root growth in germinating WT and transgenic wheat lines to ABA treatment. The (<b>A</b>) growth phenotype, (<b>B</b>) shoot length, and (<b>C</b>) primary root length of wheat seedlings germinated in the presence of different concentrations of ABA for 5 days. The data represent the mean ± SD of three biological replicates. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 10
<p>Effects of drought stress on root growth, water absorption ability, and osmotic adjustment substances contents in WT and transgenic wheat lines. The (<b>A</b>) root growth phenotype, (<b>B</b>) shoot length, and (<b>C</b>) root length of wheat seedlings before and after 20% PEG6000 treatment. (<b>D</b>) Root vitality and (<b>E</b>) AQP activity of wheat seedlings with and without 20% PEG6000 treatment for 4 days. The contents of (<b>F</b>) soluble sugar (<b>G</b>) and proline in all lines under drought stress for 5 days in soil mixture. The data represent the mean ± SD of three biological replicates. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 11
<p>Transcriptome analysis of <span class="html-italic">TaFBA1</span>-OE wheat with or without drought treatment. (<b>A</b>) The number of up-regulated and down-regulated genes and (<b>B</b>) heatmap of expression profiles of DEGs in CB037 and SCB037 and in FO3 and SFO3. These comparisons are between plants of the same genotype grown under normal and dehydration conditions. (<b>C</b>) Venn diagrams showing the DEGs between different comparisons; CB037 vs. FO3up and CB037 vs. FO3down mean genes up-regulated and down-regulated in the well-watered OE line (FO3) compared to the well-watered WT (CB037); CB037 vs. SCB037up and CB037 vs. SCB037down mean genes up-regulated and down-regulated in dehydrated CB037 compared to well-watered CB037. (<b>D</b>) The DEGs in CB037 and FO3, SCB037 and SFO3. Relative expression levels of (<b>E</b>) <span class="html-italic">TaLOX</span>, (<b>F</b>) <span class="html-italic">TaLOX1.1</span>, (<b>G</b>) <span class="html-italic">TaFAD7</span>, (<b>H</b>) <span class="html-italic">TaEXPA2</span>, (<b>I</b>) <span class="html-italic">TaCSL3-2</span>, and (<b>K</b>) <span class="html-italic">TaCSL3-4</span>. (<b>J</b>) Top 10 enriched terms among the DEGs identified from CB037/FO3 and SCB037/SFO3, respectively. The data represent the mean ± SD of three biological replicates. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">
14 pages, 2183 KiB  
Article
Study on the Effects of Microwave Heating Time and Power on the Mechanical Properties of Cemented Tailings Backfill
by Pengchu Ding, Shiheng Yan, Qinqiang Guo, Liwu Chang, Zhen Li, Changtai Zhou, Dong Han and Jie Yang
Minerals 2024, 14(9), 944; https://doi.org/10.3390/min14090944 (registering DOI) - 15 Sep 2024
Viewed by 328
Abstract
With the escalating demand for advanced and eco-friendly processing technologies in mining engineering, the potential applications of microwave heating technology in the treatment of cement tailings backfill (CTB) are expanding significantly. This research comprehensively investigates the mechanisms through which microwave irradiation duration and [...] Read more.
With the escalating demand for advanced and eco-friendly processing technologies in mining engineering, the potential applications of microwave heating technology in the treatment of cement tailings backfill (CTB) are expanding significantly. This research comprehensively investigates the mechanisms through which microwave irradiation duration and power influence the mechanical properties of CTB with varying concentrations and cement-to-sand ratios. The aim is to reveal the influencing patterns through experimental methods, providing scientific evidence for optimizing CTB treatment processes. This paper conducted microwave heating tests, uniaxial compression tests, and SEM-EDS tests on CTB. The research results indicate that heating time and power significantly enhance the early strength of CTB, with a more pronounced effect on CTB with higher concentrations and higher cement–sand ratios. When the heating time is 7 min and the heating power is 340 W, the cement hydration reaction is maximally promoted, thereby increasing the density and strength growth rate of CTB. However, excessively long heating time or overly high heating power may cause microcracks or thermal stress concentration within the CTB, adversely affecting the strength growth rate of CTB. Optimal thermal exposure duration and microwave power settings facilitate the activation of cementitious materials and the nucleation of calcium-silicate-hydrate (C-S-H) phases, thereby accelerating the compressive strength evolution of cemented tailings backfill (CTB). The outcomes of this research offer valuable insights into the deployment of microwave heating methodologies in underground mine backfilling, which are pivotal for augmenting the economic viability and environmental sustainability of mining operations. Full article
(This article belongs to the Topic New Advances in Mining Technology)
21 pages, 607 KiB  
Review
Understanding Amyotrophic Lateral Sclerosis: Pathophysiology, Diagnosis, and Therapeutic Advances
by Radu Eugen Rizea, Antonio-Daniel Corlatescu, Horia Petre Costin, Adrian Dumitru and Alexandru Vlad Ciurea
Int. J. Mol. Sci. 2024, 25(18), 9966; https://doi.org/10.3390/ijms25189966 (registering DOI) - 15 Sep 2024
Viewed by 529
Abstract
This review offers an in-depth examination of amyotrophic lateral sclerosis (ALS), addressing its epidemiology, pathophysiology, clinical presentation, diagnostic techniques, and current as well as emerging treatments. The purpose is to condense key findings and illustrate the complexity of ALS, which is shaped by [...] Read more.
This review offers an in-depth examination of amyotrophic lateral sclerosis (ALS), addressing its epidemiology, pathophysiology, clinical presentation, diagnostic techniques, and current as well as emerging treatments. The purpose is to condense key findings and illustrate the complexity of ALS, which is shaped by both genetic and environmental influences. We reviewed the literature to discuss recent advancements in understanding molecular mechanisms such as protein misfolding, mitochondrial dysfunction, oxidative stress, and axonal transport defects, which are critical for identifying potential therapeutic targets. Significant progress has been made in refining diagnostic criteria and identifying biomarkers, leading to earlier and more precise diagnoses. Although current drug treatments provide some benefits, there is a clear need for more effective therapies. Emerging treatments, such as gene therapy and stem cell therapy, show potential in modifying disease progression and improving the quality of life for ALS patients. The review emphasizes the importance of continued research to address challenges such as disease variability and the limited effectiveness of existing treatments. Future research should concentrate on further exploring the molecular foundations of ALS and developing new therapeutic approaches. The implications for clinical practice include ensuring the accessibility of new treatments and that healthcare systems are equipped to support ongoing research and patient care. Full article
(This article belongs to the Special Issue Molecular Research of Amyotrophic Lateral Sclerosis)
Show Figures

Figure 1

Figure 1
<p>Role of Chitinase as a Biomarker in ALS Pathophysiology. This image illustrates how activated microglia and astrocytes in ALS contribute to chitinase expression, which serves as a biomarker for disease progression and therapeutic monitoring.</p>
Full article ">
21 pages, 1682 KiB  
Review
Epigenetic Regulation for Heat Stress Adaptation in Plants: New Horizons for Crop Improvement under Climate Change
by Qiang Jin, Muzafaruddin Chachar, Aamir Ali, Zaid Chachar, Pingxian Zhang, Adeel Riaz, Nazir Ahmed and Sadaruddin Chachar
Agronomy 2024, 14(9), 2105; https://doi.org/10.3390/agronomy14092105 - 15 Sep 2024
Viewed by 399
Abstract
Global warming poses a significant threat to plant ecosystems and agricultural productivity, primarily through heat stress (HS), which disrupts photosynthesis, respiration, and overall plant metabolism. Epigenetic modifications, including DNA methylation, histone modifications, and RNA modifications, enable plants to dynamically and heritably adjust gene [...] Read more.
Global warming poses a significant threat to plant ecosystems and agricultural productivity, primarily through heat stress (HS), which disrupts photosynthesis, respiration, and overall plant metabolism. Epigenetic modifications, including DNA methylation, histone modifications, and RNA modifications, enable plants to dynamically and heritably adjust gene expression in response to environmental stressors. These mechanisms not only help plants survive immediate stress but also confer stress memory, enhancing their resilience to future HS events. This review explores the mechanisms underlying plant thermotolerance, emphasizing the critical role of epigenetic regulation in adapting to HS. It also highlights how DNA methylation modulates stress-responsive genes, histone modifications facilitate transcriptional memory, and RNA modifications influence mRNA stability and translation. Recent advancements in genome editing technologies, such as CRISPR-Cas9, have enabled precise modifications of epigenetic traits, offering new avenues for breeding climate-resilient crops. The integration of these modern tools with traditional breeding methods holds significant promise for developing crops with enhanced thermotolerance. Despite the potential, challenges such as the stability and heritability of epigenetic marks and the complex interplay between different epigenetic modifications need to be addressed. Future research should focus on elucidating these interactions and identifying reliable epigenetic markers for selection. By leveraging the insights gained from epigenetic studies, we can develop innovative breeding strategies to improve crop resilience and ensure sustainable agricultural productivity in the face of global warming. This review underscores the importance of epigenetic regulation in plant adaptation to heat stress and its potential to revolutionize crop breeding, offering a pathway to secure food production and sustainability under changing climatic conditions. Full article
Show Figures

Figure 1

Figure 1
<p>Epigenetic regulation pathways of heat shock response (HSR) in plants. HEAT SHOCK TRANSCRIPTION FACTOR A1s (HsfA1s), HEAT SHOCK PROTEIN 70 (HSP70) and HEAT SHOCK PROTEIN 90 (HSP90), DEHYDRATION-RESPONSIVE ELEMENT BINDING 2A (DREB2A), MULTIPROTEIN BRIDGING FACTOR 1C (MBF1C), JUNGBRUNNEN1 (JUB1), DNA POLYMERASE II SUBUNIT B3-1 (DPB3-1), NUCLEAR FACTOR Y, SUBUNIT A2 (NF-YA2), NUCLEAR FACTOR Y, SUBUNIT B3 (NF-YB3), DECREASED DNA METHYLATION 1 (DDM1), MORPHEUS MOLECULE 1 (MOM1).</p>
Full article ">Figure 2
<p>Epigenetic memory of heat stress in plants.</p>
Full article ">
17 pages, 2676 KiB  
Article
Application of a Real-Time Field-Programmable Gate Array-Based Image-Processing System for Crop Monitoring in Precision Agriculture
by Sabiha Shahid Antora, Mohammad Ashik Alahe, Young K. Chang, Tri Nguyen-Quang and Brandon Heung
AgriEngineering 2024, 6(3), 3345-3361; https://doi.org/10.3390/agriengineering6030191 (registering DOI) - 14 Sep 2024
Viewed by 243
Abstract
Precision agriculture (PA) technologies combined with remote sensors, GPS, and GIS are transforming the agricultural industry while promoting sustainable farming practices with the ability to optimize resource utilization and minimize environmental impact. However, their implementation faces challenges such as high computational costs, complexity, [...] Read more.
Precision agriculture (PA) technologies combined with remote sensors, GPS, and GIS are transforming the agricultural industry while promoting sustainable farming practices with the ability to optimize resource utilization and minimize environmental impact. However, their implementation faces challenges such as high computational costs, complexity, low image resolution, and limited GPS accuracy. These issues hinder timely delivery of prescription maps and impede farmers’ ability to make effective, on-the-spot decisions regarding farm management, especially in stress-sensitive crops. Therefore, this study proposes field programmable gate array (FPGA)-based hardware solutions and real-time kinematic GPS (RTK-GPS) to develop a real-time crop-monitoring system that can address the limitations of current PA technologies. Our proposed system uses high-accuracy RTK and real-time FPGA-based image-processing (RFIP) devices for data collection, geotagging real-time field data via Python and a camera. The acquired images are processed to extract metadata then visualized as a heat map on Google Maps, indicating green area intensity based on romaine lettuce leafage. The RFIP system showed a strong correlation (R2 = 0.9566) with a reference system and performed well in field tests, providing a Lin’s concordance correlation coefficient (CCC) of 0.8292. This study demonstrates the potential of the developed system to address current PA limitations by providing real-time, accurate data for immediate decision making. In the future, this proposed system will be integrated with autonomous farm equipment to further enhance sustainable farming practices, including real-time crop health monitoring, yield assessment, and crop disease detection. Full article
17 pages, 6795 KiB  
Article
Experimental and Modeling Analysis of Polypropylene Fiber Reinforced Concrete Subjected to Alkali Attack and Freeze–Thaw Cycling Effect
by Yuxiang Huang, Yongcheng Ji, Jingchen Wang, Zihao Wang, Bosong Yu and Siyu Zhang
Materials 2024, 17(18), 4529; https://doi.org/10.3390/ma17184529 - 14 Sep 2024
Viewed by 527
Abstract
The durability of concrete materials in harsh environmental conditions, particularly in cold regions, has garnered significant attention in civil engineering research in recent years. Concrete structures in these areas are often damaged by the combined effects of alkali–silica reaction (ASR) and freeze–thaw cycles, [...] Read more.
The durability of concrete materials in harsh environmental conditions, particularly in cold regions, has garnered significant attention in civil engineering research in recent years. Concrete structures in these areas are often damaged by the combined effects of alkali–silica reaction (ASR) and freeze–thaw cycles, leading to structural cracks and significant safety hazards. Numerous studies have demonstrated that polypropylene fiber concrete exhibits excellent crack resistance and durability, making it promising for applications in cold regions. This study elucidates the impact of alkali content on concrete durability by comparing the mechanical properties and durability of different alkali–aggregate concretes. The principal experimental methodologies employed include freeze–thaw cycle experiments, which examine patterns of mass loss; fluctuations in the dynamic modulus of elasticity; and changes in mechanical properties before and after freeze cycles. The findings indicate that increased alkali content in concrete reduces its strength and durability. At 100% alkali–aggregate content, compressive strength decreases by 35.5%, flexural strength by 32.9%, mass loss increases by 35.85%, relative dynamic elastic modulus by 39.4%, and residual strength by 97.28%, indicating higher alkali content leads to diminished durability. Additionally, this paper introduces a constitutive damage model, validated by a strong correlation with experimental stress–strain curves, to effectively depict the stress–strain relationship of concrete under varying alkali contents. This research contributes to a broader understanding of concrete durability in cold climates and guides the selection of materials for sustainable construction in such environments. Full article
(This article belongs to the Section Construction and Building Materials)
Show Figures

Figure 1

Figure 1
<p>Aggregate used in the experiment: (<b>a</b>) crushed stone; (<b>b</b>) recycled concrete; (<b>c</b>) basalt; (<b>d</b>) granite.</p>
Full article ">Figure 2
<p>Gel precipitation of (<b>a</b>) C100, (<b>b</b>) R100, (<b>c</b>) B100, and (<b>d</b>) G100 (ordered from <b>left</b> to <b>right</b>) after soaking in 0.1 mol/L NaOH solution.</p>
Full article ">Figure 3
<p>Particle size distribution curve of aggregate.</p>
Full article ">Figure 4
<p>Parallel bar system.</p>
Full article ">Figure 5
<p>Uniaxial stress–strain curve of recycled concrete.</p>
Full article ">Figure 6
<p>The relationship between parameters and alkali–aggregate content r: (<b>a</b>) variation of parameter a with alkali aggregate; (<b>b</b>) variation of parameter b with alkali aggregate; (<b>c</b>) variation of E with r alkali aggregate.</p>
Full article ">Figure 7
<p>Test curve and theoretical calculation curve: (<b>a</b>) The fitting curve and C100 test curve; (<b>b</b>) The fitting curve and R30 test curve; (<b>c</b>) The fitting curve and R60 test curve; (<b>d</b>) The fitting curve and R100 test curve.</p>
Full article ">Figure 8
<p>Concrete compressive and flexural strength of each group.</p>
Full article ">Figure 9
<p>The mass loss rate of concrete: (<b>a</b>) mass loss rate of R group; (<b>b</b>) mass loss rate of B group; (<b>c</b>) mass loss rate of G group.</p>
Full article ">Figure 10
<p>Relative dynamic elastic modulus loss rate of concrete: (<b>a</b>) relative dynamic elastic modulus loss rate of R group; (<b>b</b>) relative dynamic elastic modulus loss rate of B group; (<b>c</b>) relative dynamic elastic modulus loss rate of G group.</p>
Full article ">Figure 11
<p>The strength loss rate of concrete: (<b>a</b>) strength loss rate of the R group; (<b>b</b>) strength loss rate of the B group; (<b>c</b>) strength loss rate of the G group.</p>
Full article ">Figure 12
<p>Flexural strength loss rate prediction curve at different freeze–cycle times.</p>
Full article ">
14 pages, 4957 KiB  
Article
Toxicity Evaluation of Potassium Sorbate In Vivo with Drosophila Melanogaster
by Xubo Zhang, Qian Zhang, Xiaoxuan Song, Wanchen Yang, Andi Cheng, Jianzhen Zhang and Wei Dong
Insects 2024, 15(9), 703; https://doi.org/10.3390/insects15090703 - 14 Sep 2024
Viewed by 327
Abstract
Potassium sorbate (PS) is a preservative widely used in the food, pharmaceutical, and cosmetics industries. Improper and careless use of PS can lead to various health issues and potential environmental problems. Drosophila is capable of making rapid and sensitive responses to stress or [...] Read more.
Potassium sorbate (PS) is a preservative widely used in the food, pharmaceutical, and cosmetics industries. Improper and careless use of PS can lead to various health issues and potential environmental problems. Drosophila is capable of making rapid and sensitive responses to stress or other stimuli. Here we utilized Drosophila as a model organism to evaluate the potential toxicity of PS. Our study revealed that PS ingestion reduced the lifespan and fecundity of Drosophila. In addition, excessive PS ingestion led to cell apoptosis and ROS accumulation in the midgut. Furthermore, PS intake also enhanced the mitophagy of midgut cells. Strikingly, PS affected the cell differentiation progression as well, leading to the production of more enteroendocrine (EE) cells. We further demonstrated that the expression of notch (N), a vital player in intestinal stem cell (ISC) differentiation, was down-regulated in the midgut. This indicates that the differentiation progression was affected potentially by repressing the N expression. Full article
(This article belongs to the Section Insect Physiology, Reproduction and Development)
Show Figures

Figure 1

Figure 1
<p>The impact of PS intake on the lifespan and fecundity of <span class="html-italic">Drosophila</span>. (<b>A</b>) PS intake led to a reduction in <span class="html-italic">Drosophila</span> longevity. (<b>B</b>) The LT50 of flies fed with PS. (<b>C</b>) The overall quantity of eggs laid over a span of 5 days. <span class="html-italic">p</span> ≤ 0.05 was considered significant (*), <span class="html-italic">p</span> ≤ 0.001 (***), and <span class="html-italic">p</span> ≤ 0.0001 (****).</p>
Full article ">Figure 2
<p>PS impacts the cell membrane of the midgut, while not affecting the pH. (<b>A</b>) UAS-CD8-RFP was driven by c601-Gal4 to mark the cell membrane of the midgut. The cell membrane in the midguts of flies fed with 0.1% and 0.5% PS appears fuzzy, specifically in the midguts of 0.5% PS-fed flies. (<b>B</b>) An acidic area, indicated by the yellow color, is noticeable in the center of control midgut, while other regions appeared blue. BPB staining demonstrated that the midgut’s pH was not visibly influenced by 0.1% and 0.5% PS ingestion.</p>
Full article ">Figure 3
<p>PS ingestion resulted in cell apoptosis in the midgut. (<b>A</b>) The Trypan blue staining exhibited induced cell death in the midguts fed with 0.1% and 0.5% PS. (<b>B</b>–<b>D</b>) The puc-lacZ staining also demonstrated that cell death was induced by PS ingestion.</p>
Full article ">Figure 4
<p>PS ingestion resulted in ROS accumulation in the midgut. (<b>A</b>–<b>C</b>) The figure shows ROS detection in the midguts of flies fed on standard medium or PS medium (0.1% and 0.5%). Scale bar = 50 μm.</p>
Full article ">Figure 5
<p>PS ingestion induced mitophagy in the midgut. (<b>A</b>–<b>C</b>) The midguts from flies that were fed on 0.1% and 0.5% PS medium exhibited more severe mitophagy when compared with the control group. White arrows indicate the red-only punctas.</p>
Full article ">Figure 6
<p>The gene expression levels in the midguts of flies fed with standard medium or PS medium. (<b>A</b>) The expression levels of genes related to cell membrane integrity. (<b>B</b>) The expression levels of genes related with cell apoptosis or mitophagy. All data are reported as means ± SEM of three independent biological replications. The asterisks indicate significance differences between the control and PS-fed groups (<span class="html-italic">p</span> ≤ 0.01, **), while “ns” means no statistically significant differences.</p>
Full article ">Figure 7
<p>PS ingestion alters ISCs differentiation trajectory. (<b>A</b>–<b>C</b>) EE cells in the midgut from flies fed with 0.1% and 0.5% PS medium were labeled by Prospero antibody staining. (<b>D</b>) Quantitative analysis of the Prospero antibody-labeled EE cells, the number of EE cells were increased in the midguts of the flies fed with PS. All data are reported as means ± SEM of three independent biological replications. <span class="html-italic">p</span> ≤ 0.05 was considered significant (*) and <span class="html-italic">p</span> ≤ 0.0001 (****).</p>
Full article ">Figure 8
<p>PS ingestion led to the reduction of Notch signaling. (<b>A</b>) The N-GFP in the midgut of flies fed with the standard medium or PS medium (0.1% and 0.5%). Scale bar = 50 μm. (<b>B</b>) PS ingestion did not significantly influence the number of N-GFP clones. (<b>C</b>) PS ingestion notably reduced the size of N-GFP clones. (<b>D</b>) RT-qPCR result showed that the <span class="html-italic">notch</span> expression was down-regulated in midgut of 0.5% PS-fed flies. All data are reported as means ± SEM of three independent biological replications. The asterisks indicate significance differences between the control and PS-fed groups (<span class="html-italic">p</span> ≤ 0.01, **; <span class="html-italic">p</span> ≤ 0.0001 ****), while “ns” means no statistically significant differences.</p>
Full article ">
15 pages, 1320 KiB  
Article
Phenylalanine, Cysteine, and Sodium Selenate Alleviate Chilling Injury in Cape Gooseberry (Physalis peruviana L.) Seedlings by Enhancing Antioxidant Activities and Membrane Stability
by Arezoo Akbari, Taher Barzegar, Vali Rabiei and Silvana Nicola
Horticulturae 2024, 10(9), 978; https://doi.org/10.3390/horticulturae10090978 (registering DOI) - 14 Sep 2024
Viewed by 196
Abstract
Low temperature is a major environmental factor that negatively affects the growth and productivity of plants, such as the tropical fruit Cape gooseberry (Physalis peruviana L.), which is susceptible to cold stress. Therefore, to investigate the effect of the amino acid L-phenylalanine [...] Read more.
Low temperature is a major environmental factor that negatively affects the growth and productivity of plants, such as the tropical fruit Cape gooseberry (Physalis peruviana L.), which is susceptible to cold stress. Therefore, to investigate the effect of the amino acid L-phenylalanine (Phe), L-cysteine (Cys), or sodium selenite (Se) on enhancing antioxidant activities, experiments were conducted on the phenolic compounds, proline content, and membrane stability of Cape gooseberry seedlings under low-temperature stress. The seedlings were exposed for 48 h to a low temperature (4 °C) followed by 24 h of optimal growth conditions. In seedlings treated with Se, we found a high relative water content, good membrane integrity, low ion leakage, and hydrogen peroxide. Additionally, this treatment led to the improvement of photosynthetic pigments and antioxidant activity. The analysis of seedlings under cold stress showed that the Phe enhanced the stomatal conductance and phenol content. Furthermore, low concentrations of Cys resulted in the production of proline and flavonoids, which reduced the negative effects of environmental stress on seedlings and maintained cell membrane integrity. Overall, in this experiment, the use of Se and low concentrations of Cys had a positive effect on the amount of antioxidant compounds, which improved seedling growth under stress conditions. Full article
(This article belongs to the Section Biotic and Abiotic Stress)
14 pages, 5519 KiB  
Article
Research on the Energy-Absorbing and Cushioning Performance of a New Half-Bowl Ball Rubber Body in Tunnel Support
by Jian Ma, Yaomeng Xiao, Bin Ma, Canguang Zheng, Xiangpeng Hu, Dan Tian, Mingchao Du and Kun Zhang
Processes 2024, 12(9), 1981; https://doi.org/10.3390/pr12091981 - 14 Sep 2024
Viewed by 216
Abstract
As coal mine underground operating conditions are harsh, strengthening and optimizing the support structure is conducive to the safety of mining work and personnel. Currently, underground support devices face problems such as poor environmental adaptability and unbalanced performance of shockproof and energy absorption. [...] Read more.
As coal mine underground operating conditions are harsh, strengthening and optimizing the support structure is conducive to the safety of mining work and personnel. Currently, underground support devices face problems such as poor environmental adaptability and unbalanced performance of shockproof and energy absorption. At the same time, the energy absorption mechanism and impact dynamic analysis of the support structure are still imperfect. This paper proposes a simple and effective bionic half-bowl spherical rubber energy-absorbing structure based on the actual production needs of coal mines, with energy-absorbing rubber as the main structural interlayer. A combination of experimental testing and simulation was used to reveal the dynamic response and mechanism of simulated energy absorption of a half-bowl-shaped rubber layer under different working conditions. Abaqus software was used to simulate and analyze the dynamic response of the half-bowl spherical rubber structure under the impact condition, and the simulation data were compared with the experimental results. In addition, the relationship between energy absorption and stress at the rubber structure and the base plate under different impact velocities was investigated. The results show that the simulated and experimental results of the rubber structure have almost the same pressure vs. time trend within 0.1 s at an impact velocity of 64 m/s, and there is no significant wear on the rubber surface after impact. Due to the energy-absorbing effect of the rubber structure, the maximum stress of the bottom member plate-2 of the mechanism is lower than 9 × 104 N. The maximum amount of compression of the half-bowl ball is 37.56 mm at an impact velocity of 64 m/s. The maximum amount of compression of the half-bowl ball is 37.56 mm. Full article
(This article belongs to the Section Materials Processes)
Show Figures

Figure 1

Figure 1
<p>Buffer energy-absorbing device overall structure and assembly: (<b>a</b>) cushioning energy absorbers; (<b>b</b>) secondary telescopic disc spring-cushioned monolithic columns; (<b>c</b>) half-bowl-shaped cushioning and energy-absorbing structures; (<b>d</b>) overall installation effect diagram.</p>
Full article ">Figure 2
<p>Half-bowl rubber energy-absorbing structure.</p>
Full article ">Figure 3
<p>Energy-absorbing simulation model.</p>
Full article ">Figure 4
<p>Impact test bench: (<b>a</b>) schematic diagram of the impact instrument; (<b>b</b>) physical diagram of the impact instrument.</p>
Full article ">Figure 5
<p>Stress diagram of the rubber structure at plate-0 velocity of 64 m/s: (<b>a</b>) Step 2; (<b>b</b>) Step 20; (<b>c</b>) Step 30 and its (<b>d</b>) top view.</p>
Full article ">Figure 6
<p>Stress versus time curves for half-bowl spherical rubber structures.</p>
Full article ">Figure 7
<p>Stress versus time curves for plate-2.</p>
Full article ">Figure 8
<p>Variation of absorbed energy in rubber structures at different impact velocities.</p>
Full article ">Figure 9
<p>Maximum deformation of the half-bowl rubber structure under different impact force conditions: (<b>a</b>) the relationship between the rubber compression height and the stress of plate-2; (<b>b</b>) simulation diagram of rubber compression height.</p>
Full article ">
15 pages, 4761 KiB  
Article
Photosynthetic Performance and Heterogeneous Anatomical Structure in Prunus humilis under Saline–Alkaline Stress
by Yongjiang Sun, Xiang Wang, Qiwen Shao, Qi Wang, Siyuan Wang, Ruimin Yu, Shubin Dong, Zhiming Xin, Huijie Xiao and Jin Cheng
Agriculture 2024, 14(9), 1606; https://doi.org/10.3390/agriculture14091606 - 14 Sep 2024
Viewed by 189
Abstract
Prunus (P.) humilis is a small woody shrub that has been widely planted in northern China due to its high nutritional value and resistance to environmental abiotic stress. However, little information about the responses of photosynthetic performance and the anatomical structure of P. [...] Read more.
Prunus (P.) humilis is a small woody shrub that has been widely planted in northern China due to its high nutritional value and resistance to environmental abiotic stress. However, little information about the responses of photosynthetic performance and the anatomical structure of P. humilis to saline–alkaline stress (SAS) under field conditions is available. Here, we investigated the behavior of the photosynthetic apparatus of P. humilis by measuring the chlorophyll fluorescence parameters under moderate (MS) and severe (SS) saline–alkaline stress and analyzing their relationship to leaf anatomical traits. The results showed that SAS significantly decreased the net photosynthetic rate (An) but increased the substomatal CO2 concentration (Ci). The maximum photochemical quantum yield of PSII (Fv/Fm) and the efficient quantum yield of PSII [Y(II)] decreased under MS and SS conditions, and this decrease was greater in the distal (tip) than in the proximal (base) leaf. Compared to the leaf tip, the base of P. humilis leaves seemed to have a stronger ability to cope with MS, as was made evident by the increased quantum yield of regulated energy dissipation in PSII [Y(NPQ)] and decreased excitation pressure (1-qP). Under MS and SS conditions, the shapes of the chlorophyll a fluorescence transient (OJIP) changed markedly, accompanied by decreased PSII acceptor-side and donor-side activities. The palisade–spongy tissue ratio (PT/ST) increased significantly with increasing stress and showed a significant correlation with the chlorophyll fluorescence parameters in the leaf base. These results suggested that the activity of PSII electron transfer in the upper leaf position tended to be more sensitive to saline–alkaline stress, and a chlorophyll fluorescence analysis proved to be a good technique to monitor impacts of saline–alkaline stress on photosynthetic function, which may reflect the non-uniformity of leaf anatomy. In addition, among the anatomical structure parameters, the palisade–spongy tissue ratio (PT/ST) can be used as a sensitive indicator to reflect the non-uniform of photosynthetic function and leaf anatomy under stress. Full article
(This article belongs to the Section Crop Production)
Show Figures

Figure 1

Figure 1
<p>Study area located in Dengkou County, Inner Mongolia Autonomous Region, China, and sampling points.</p>
Full article ">Figure 2
<p>Changes in An (<b>A</b>), Gs (<b>B</b>), Ci (<b>C</b>), and LS (<b>D</b>) in <span class="html-italic">P. humilis</span> leaves under control and SAS (moderate, MS; and severe, SS) conditions. Significant differences between leaves subjected to different stress conditions were examined (<span class="html-italic">p</span> &lt; 0.05). Different letters indicate significant differences compared to the control.</p>
Full article ">Figure 3
<p>Representative chlorophyll fluorescence images of Fo and Fm in <span class="html-italic">P. humilis</span> leaves under dark-adapted control and SAS (moderate, MS; and severe, SS) conditions. On the right side of the image are color codes ranging from black to red.</p>
Full article ">Figure 4
<p>Changes in SPAD (<b>A</b>) and Fv/Fm (<b>B</b>) in the tip and base of <span class="html-italic">P. humilis</span> leaves under control and SAS (moderate, MS; and severe, SS) conditions. Significant differences are indicated by different lowercase letters (<span class="html-italic">p</span> &lt; 0.05) between positions in leaves subjected to different stress conditions. The means and SEs were calculated from a total of 6–8 plants.</p>
Full article ">Figure 5
<p>Changes in Y(II) (<b>A</b>), Y(NPQ) (<b>B</b>), and Y(NO) (<b>C</b>) in the tip and base of <span class="html-italic">P. humilis</span> under control and SAS (moderate, MS; and severe, SS) conditions. Different lowercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) between positions in leaves subjected to different stress conditions. The means and SEs were calculated from a total of 6–8 plants.</p>
Full article ">Figure 6
<p>Changes in PSII excitation pressure (1-qP) in the tip and base of <span class="html-italic">P. humilis</span> under control and SAS (moderate, MS; and severe, SS) conditions. Significant differences are indicated by different lowercase letters (<span class="html-italic">p</span> &lt; 0.05) between positions in leaves subjected to different stress conditions. The means and SEs were calculated from a total of 6–8 plants.</p>
Full article ">Figure 7
<p>Changes in the OJIP transients in the tip (<b>A</b>,<b>C</b>) and base (<b>B</b>,<b>D</b>) of <span class="html-italic">P. humilis</span> under control and SAS (moderate, MS; and severe, SS) conditions. ΔVt (<b>C</b>,<b>D</b>) was obtained by subtracting the kinetics of control leaves from the kinetics of stressed leaves. O indicates the O step at about 20 μs; K indicates the Kstep at about 300 μs; J indicates the J step at about 2 ms; I indicates the I step at about 30 ms; P indicates the P step at about 1 s. The average of six independent measurements is used for each curve.</p>
Full article ">Figure 8
<p>Changes in Wk (<b>A</b>), RC/CSo (<b>B</b>), Ψo (<b>C</b>), and PIabs (<b>D</b>) in the tip and base of <span class="html-italic">P. humilis</span> under control and SAS (moderate, MS; and severe, SS) conditions. Different lowercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) between positions in leaves subjected to different stress conditions. The means and SEs were calculated from a total of 6–8 plants.</p>
Full article ">Figure 9
<p>Changes in leaf structure images in the tip (<b>A</b>–<b>C</b>) and base (<b>D</b>–<b>F</b>) under control and SAS (moderate, MS; and severe, SS) conditions.</p>
Full article ">Figure 10
<p>Relationships between chlorophyll fluorescence parameters and anatomical structure characteristics of <span class="html-italic">P. humilis</span>. (<b>A</b>) Leaf tip; (<b>B</b>) leaf base. Asterisk indicates significant correlations (<span class="html-italic">p</span> &lt;  0.05).</p>
Full article ">
Back to TopTop