Search Results (177)

Climate change is one of the biggest challenges today. An increasing population accelerates the construction of concrete houses and the use of air conditioners, thereby leading to an increase in energy consumption. When the walls of buildings are well-designed and insulated, energy consumption can be reduced. Therefore, it is important to measure the thermal performance of wall systems accurately. The existing traditional methods of measuring R- and U-values provide acceptable solutions for steady-state controlled, uncontrolled or transient state-controlled conditions. However, a need to develop a novel approach for transient state-uncontrolled realistic conditions has been identified. The present study involves both experimental and numerical investigations. An in situ model room with dimensions of 1.60 m × 1.73 m × 1.50 m was built for the experimental work, and a series of experiments were conducted. For numerical work, two models using Ansys Fluent 2021/2022 and MATLAB Simulink 2021/2022 were developed. The real-time experimental data were fed into numerical models to predict the thermal behavior of the wall system. The results include the evaluation of a concept called ‘Time-Lag’ for all three models. ‘Time-Lag’ is the time taken for the heat energy to flow across the wall system. The Time-Lag for the experimental model was 8 h 45 min, while for MATLAB and Ansys models, it was 8 h 22 min. (average) and 7 h 30 min, respectively. Minor variations validate the accuracy of the numerical models. Further, a novel method using a new parameter in building systems called ‘thermal impedance Z-value’ was developed to estimate the real-time thermal performance of walls using MATLAB Simulink. The Z-value measures the ability of a wall system to resist the flow of heat (thermal resistance, R-value) combined with its ability to store heat energy (thermal capacitance, C_th-value). It is evaluated for steady-state and dynamic (transient) systems. For the steady-state system, the Z-values on the outer and inner walls were 18.2683 K/W and 18.6761 K/W, respectively with a minor difference of 0.4078 K/W at the end of 72 h. For the dynamic system, the Z-value did not reach a constant value and fluctuated in a particular pattern during 24 h of the solar cycle with average values of 3.2969 K/W on the outer and 1.2886 K/W on the inner walls at the end of 72 h, thus presenting more accurate and realistic thermal performance results of a wall system. Full article

Chlamydia trachomatis remains a major global health problem with increasing infection rates, requiring innovative vaccine solutions. Modified Vaccinia Virus Ankara (MVA) is a well-established, safe and highly immunogenic vaccine vector, making it a promising candidate for C. trachomatis vaccine development. In this study, we evaluated two novel MVA-based recombinant vaccines expressing spCTH522 and CTH522:B7 antigens. Our results show that while both vaccines induced CD4⁺ T-cell responses in C57BL/6J mice, they failed to generate antigen-specific systemic CD8⁺ T cells. Only the membrane-anchored CTH522 elicited strong IgG2b and IgG2c antibody responses. In an HLA transgenic mouse model, both recombinant MVAs induced Th1-directed CD4⁺ T cell and multifunctional CD8⁺ T cells, while only the CTH522:B7 vaccine generated antibody responses, underscoring the importance of antigen localization. Collectively, our data indicate that distinct antigen formulations can induce different immune responses depending on the mouse strain used. This research contributes to the development of effective vaccines by highlighting the importance of careful antigen design and the selection of appropriate animal models to study specific vaccine-induced immune responses. Future studies should investigate whether these immune responses provide protection in humans and should explore different routes of immunization, including mucosal and systemic immunization. Full article

Polyphosphate (polyP) is an evolutionary ancient inorganic molecule widespread in biology, exerting a broad range of biological activities. The intracellular polymer serves as an energy storage pool and phosphate/calcium ion reservoir with implications for basal cellular functions. Metabolisms of the polymer are well understood in procaryotes and unicellular eukaryotic cells. However, functions, regulation, and association with disease states of the polymer in higher eukaryotic species such as mammalians are just beginning to emerge. The review summarises our current understanding of polyP metabolism, the polymer’s functions, and methods for polyP analysis. In-depth knowledge of the pathways that control polyP turnover will open future perspectives for selective targeting of the polymer. Full article

Human cytomegalovirus (HCMV) infection is common in tumor tissues across different types of cancer. While HCMV has not been recognized as a cancer-causing virus, numerous studies hint at its potential role in cancer development where its presence in various cancers corresponds with the hallmarks of cancer. Herein, we discuss and demonstrate that high-risk HCMV-DB and BL strains have the potential to trigger transformation in epithelial cells, including human mammary epithelial cells (HMECs), ovarian epithelial cells (OECs), and prostate epithelial cells (PECs), through the generation of polyploid giant cancer cells (PGCCs). A discussion is provided on how HCMV infection creates a cellular environment that promotes oncogenesis, supporting the continuous growth of CMV-transformed cells. The aforementioned transformed cells, named CTH, CTO, and CTP cells, underwent giant cell cycling with PGCC generation parallel to dedifferentiation, displaying stem-like characteristics and an epithelial–mesenchymal transition (EMT) phenotype. Furthermore, we propose that giant cell cycling through PGCCs, increased EZH2 expression, EMT, and the acquisition of malignant traits represent a deleterious response to the cellular stress induced by high-risk oncogenic HCMV strains, the latter being the origin of the transformation process in epithelial cells upon HCMV infection and leading to adenocarcinoma of poor prognosis. Full article

The liquid-phase hydrogenation of muconic acid (MA) to produce bio-adipic acid (AdA) is a prominent environmentally friendly chemical process, that can be achieved through two distinct methodologies: catalytic direct hydrogenation using molecular hydrogen (H₂), or catalytic transfer hydrogenation utilizing a hydrogen donor. In this study, both approaches were explored, with formic acid (FA) selected as the hydrogen source for the latter method. Palladium-based catalysts were chosen for these processes. Metal’s nanoparticles (NPs) were supported on high-temperature heat-treated carbon nanofibers (HHT-CNFs) due to their known ability to enhance the stability of this metal catalyst. To assess the impact of support functionalization on catalyst stability, the HHT-CNFs were further functionalized with phosphorus and oxygen to obtain HHT-P and HHT-O, respectively. In the hydrogenation reaction, catalysts supported on functionalized supports exhibited higher catalytic activity and stability compared to Pd/HHT, reaching an AdA yield of about 80% in less than 2 h in batch reactor. The hydrogen-transfer process also yielded promising results, particularly with the 1%Pd/HHT-P catalyst. This work highlights the efficacy of support functionalization in improving catalyst performance, particularly when formic acid is used as a safer and more cost-effective hydrogen donor in the hydrogen-transfer process. Full article

During estrus, the poll glands of male Bactrian Camels (Camelus Bactrianus) become slightly raised, exuding a large amount of pale yellow watery secretion with a characteristic odor that may contain hydrogen sulfide (H₂S). However, whether H₂S can be synthesized in the poll glands of male Bactrian Camels and its role in inducing camel estrus remains unclear. This study aimed to identify differentially expressed proteins (DEPs) and signaling pathways in the poll gland tissues of male Bactrian Camels using data independent acquisition (DIA) proteomics. Additionally, gas chromatography–mass spectrometry (GC-MS) was performed to identify differentially expressed metabolites (DEMs) in the neck hair containing secretions during estrus in male Bactrian Camels, to explore the specific expression patterns and mechanisms in the poll glands of camels during estrus. The results showed that cystathionine-γ-lyase (CTH) and cystathionine-β-synthase (CBS), which are closely related to H₂S synthesis in camel poll glands during estrus, were mainly enriched in glycine, serine, and threonine metabolism, amino acid biosynthesis, and metabolic pathways. In addition, both enzymes were widely distributed and highly expressed in the acinar cells of poll gland tissues in camels during estrus. Meanwhile, the neck hair secretion contains high levels of amino acids, especially glycine, serine, threonine, and cystathionine, which are precursors for H₂S biosynthesis. These results demonstrate that the poll glands of male Bactrian Camels can synthesize and secrete H₂S during estrus. This study provides a basis for exploring the function and mechanism of H₂S in the estrus of Bactrian Camels. Full article

Background: Mitochondria play a crucial role in adapting to fluctuating energy demands, particularly in various heart diseases. This study investigates mitochondrial morphology near intercalated discs in left ventricular (LV) heart tissues, comparing samples from patients with sinus rhythm (SR), atrial fibrillation (AF), dilated cardiomyopathy (DCM), and ischemic cardiomyopathy (ICM). Methods: Transmission electron microscopy was used to analyze mitochondria within 0–3.5 μm and 3.5–7 μm of intercalated discs in 9 SR, 10 AF, 9 DCM, and 8 ICM patient samples. Parameters included mean size in µm² and elongation, count, percental mitochondrial area in the measuring frame, and a conglomeration score. Results: AF patients exhibited higher counts of small mitochondria in the LV myocardium, resembling SR. DCM and ICM groups had fewer, larger, and often hydropic mitochondria. Accumulation rates and percental mitochondrial area were similar across groups. Significant positive correlations existed between other defects/size and hydropic mitochondria and between count/area and conglomeration score, while negative correlations between count and size/other defects and between hydropic mitochondria and count could be seen as well. Conclusion: Mitochondrial parameters in the LV myocardium of AF patients were similar to those of SR patients, while DCM and ICM displayed distinct changes, including a decrease in number, an increase in size, and compromised mitochondrial morphology. Further research is needed to fully elucidate the pathophysiological role of mitochondrial morphology in different heart diseases, providing deeper insights into potential therapeutic targets and interventions. Full article

Sea fog is a societally relevant phenomenon that occurs under the influence of specific oceanic and atmospheric conditions including aerosol conditions. The Yellow Sea region in China regularly experiences sea fog events, of varying intensity, that impact coastal regions and maritime activities. The occurrence and structure of fog are impacted by the concentration of aerosols in the air where the fog forms. Along with industrial development, air pollution has become a serious environmental problem in Northeastern China. These higher pollution levels are confirmed by various satellite remote sensing instruments including the Moderate Resolution Imaging Spectroradiometer (MODIS) aboard the Aqua satellite that observes aerosol and cloud properties. These observations show a clear influence of aerosol loading over the Yellow Sea region, which can impact regional sea fog. In this study, high-resolution data sets from MODIS Aqua L2 are used to investigate the relationships between cloud properties and aerosol features. Using a bi-variate comparison method, we find that, for most cases, larger values of COT (cloud optical thickness) are related to both a smaller DER (droplet effective radius) and higher CTH (cloud top height). However, in the cases where fog is thinner with many zero values in CTH, the larger COT is related to both a smaller DER and CTH. For fog cases where the aerosol type is dominated by smoke (e.g., confirmed fire activities in the East China Plain), the semi-direct effect is indicated and may play a role in determining fog structure such that a smaller DER corresponds with thinner fog and smaller COT values. Full article

This paper proposes a ResGA-Net algorithm for cloud top height (CTH) retrieval using FY-4A satellite data. The algorithm utilizes genetic algorithms for data selection and employs a residual module-based neural network for modeling. It takes the spectral channel data from the FY-4A satellite as input features and uses CTH extracted from ground-based millimeter-wave cloud radar reflectivity as the target. By combining the large observation scale of the FY-4A satellite and the high accuracy of ground-based cloud radar observations, the model can generate satellite CTH products with higher precision. To validate the effectiveness of the algorithm, experiments were conducted using data from the Beijing area spanning from January 2020 to January 2022. The experimental results show that the metrics of the proposed ResGA-Net outperform those of various contrastive algorithms, and compared to the original FY-4A CTH product, the RMSE and MAE have decreased by 37.89% and 34.77%, while the PCC and SRCC have increased by 11.17% and 9.47%, respectively, demonstrating the superiority of the proposed method presented in this paper. Full article

A novel series of 1,2,4-triazole analogues of caffeic acid was designed, synthesized, characterized, and assessed for their capacity to inhibit DHFR, as well as their anticancer and antimicrobial properties. A molecular docking analysis was conducted on DHFR, utilizing PDB IDs 1U72 and 2W9S, aiming to design anticancer and antimicrobial drugs, respectively. Among all the synthesized derivatives, compound CTh7 demonstrated the highest potency as a DHFR inhibitor, with an IC₅₀ value of 0.15 μM. Additionally, it exhibited significant cytotoxic properties, with an IC₅₀ value of 8.53 µM. The molecular docking analysis of the CTh7 compound revealed that it forms strong interactions with key residues of homo sapiens DHFR such as Glu30, Phe34, Tyr121, Ile16, Val115, and Phe31 within the target protein binding site and displayed excellent docking scores and binding energy (−9.9; −70.38 kcal/mol). Additionally, synthesized compounds were screened for antimicrobial properties, revealing significant antimicrobial potential against bacterial strains and moderate effects against fungal strains. Specifically, compound CTh3 exhibited notable antibacterial efficacy against Staphylococcus aureus (MIC = 5 µM). Similarly, compound CTh4 demonstrated significant antibacterial activity against both Escherichia coli and Pseudomonas aeruginosa, with MIC values of 5 µM for each. A docking analysis of the most active antimicrobial compound CTh3 revealed that it forms hydrogen bonds with Thr121 and Asn18, a π–cation bond with Phe92, and a salt bridge with the polar residue Asp27. Full article

Background: Ischemic stroke is the second, and pulmonary embolism (PE) is the third most common cardiovascular cause of death after myocardial infarction. Data regarding risk factors for ischemic stroke in patients with acute PE are limited. Methods: Patients were selected by screening the German nationwide in-patient sample for PE (ICD-code I26) and were stratified by ischemic stroke (ICD code I63) and compared. Results: The nationwide in-patient sample comprised 346,586 hospitalized PE patients (53.3% females) in Germany from 2011 to 2014; among these, 6704 (1.9%) patients had additionally an ischemic stroke. PE patients with ischemic stroke had a higher in-hospital mortality rate than those without (28.9% vs. 14.5%, p < 0.001). Ischemic stroke was independently associated with in-hospital death (OR 2.424, 95%CI 2.278–2.579, p < 0.001). Deep venous thrombosis and/or thrombophlebitis (DVT) combined with heart septal defect (OR 24.714 [95%CI 20.693–29.517], p < 0.001) as well as atrial fibrillation/flutter (OR 2.060 [95%CI 1.943–2.183], p < 0.001) were independent risk factors for stroke in PE patients. Systemic thrombolysis was associated with a better survival in PE patients with ischemic thrombolysis who underwent cardio-pulmonary resuscitation (CPR, OR 0.55 [95%CI 0.36–0.84], p = 0.006). Conclusions: Ischemic stroke did negatively affect the survival of PE. Combination of DVT and heart septal defect and atrial fibrillation/flutter were strong and independent risk factors for ischemic stroke in PE patients. In PE patients with ischemic stroke, who had to underwent CPR, systemic thrombolysis was associated with improved survival. Full article

Background: The use of veno-venous extracorporeal membrane oxygenation (vv-ECMO) in acute lung failure has witnessed a notable increase. The PiCCO system is frequently used for advanced hemodynamic monitoring in this cohort. Our study aimed to investigate whether the choice of indicator injection site (jugular vs. femoral) in patients undergoing vv-ECMO therapy affects transpulmonary thermodilution (TPTD) measurements using the PiCCO^® device (Pulsion Medical Systems SE, Munich, Germany). Methods: In a retrospective single-center analysis, we compared thermodilution-derived hemodynamic parameters after simultaneous jugular and femoral injections in 28 measurements obtained in two patients with respiratory failure who were undergoing vv-ECMO therapy. Results: Elevated values of the extravascular lung water index (EVLWI), intrathoracic blood volume index (ITBVI) and global end-diastolic volume index (GEDVI) were observed following femoral indicator injection compared to jugular indicator injection (EVLWI: 29.3 ± 10.9 mL/kg vs. 18.3 ± 6.71 mL/kg, p = 0.0003; ITBVI: 2163 ± 631 mL/m² vs. 806 ± 125 mL/m², p < 0.0001; GEDVI: 1731 ± 505 mL/m² vs. 687 ± 141 mL/m², p < 0.0001). The discrepancy between femoral and jugular measurements exhibited a linear correlation with extracorporeal blood flow (ECBF). Conclusions: In a PiCCO®-derived hemodynamic assessment of patients on vv-ECMO, the femoral indicator injection, as opposed to the jugular injection, resulted in an overestimation of all index parameters. This discrepancy can be attributed to mean transit time (MTt) and downslope time-dependent (DSt) variations in GEDVI and cardiac function index and is correlated with ECBF. Full article

In this study, we investigate the seasonal and diurnal variations in cloud occurrence frequency, as well as cloud vertical structure (CVS) characteristics under different seasons and precipitation intensities over the Guangzhou region in South China, based on the analysis of millimeter-wave cloud radar (MMCR) and ground automatic weather station rainfall observations from May 2019 to August 2021. The results showed that the occurrence frequency of clouds exhibits a bimodal distribution throughout the year, with peaks in March to June and October, reaching its highest occurrence in May at approximately 80% and its lowest from December to February at around 40%. Additionally, there are distinct diurnal variations in occurrence frequency, with the lowest rates occurring around 0005 LST, rapidly increasing after 0006 LST, and peaking during the afternoon to evening hours. Cloud top height (CTH) shows bimodal distributions during the pre-flood and post-flood seasons. The most frequently occurring range of CTH during the pre-flood season is below 3 km, accounting for approximately 43%, while during the post-flood season, it ranges from 11 to 14 km, constituting about 37%. For precipitation clouds, CTH can extend beyond 12 km, with the radar reflectivity decreasing gradually with increasing height. The highest frequencies of radar echoes are observed below 2 km and between 4 and 7 km, exhibiting clear diurnal variations, with echoes mainly below 2 km and between 4 to 6 km during the early morning, intensifying and shifting to higher altitudes during the day and reaching their maximum below 4 km during the afternoon to nighttime hours, while both the frequency and intensity increase in the height range of 4 to 12 km. Vertical profiles of radar reflectivity and cloud ice/liquid water content (IWC/LWC) exhibit similar trends under different precipitation intensities. The main differences are observed below 4 km, where both radar reflectivity and IWC/LWC generally increase with increasing precipitation intensity. These findings contribute to a better understanding of cloud characteristics in the South China region, enhance the accuracy of model simulations, and provide a scientific basis for accurate forecasting and warning of meteorological disasters. Full article

This study aimed to investigate the characteristic choroidal changes in patients with diabetic retinopathy and identify factors affecting choroidal thickness (CTh), choroidal vascular index (CVI), and choriocapillaris flow. We retrospectively analyzed 79 eyes of 48 patients with diabetes between August 2021 and February 2022. We collected laboratory data, including HbA1c, serum creatinine, blood urea nitrogen, triglyceride, total cholesterol, high-density lipoprotein, and low-density lipoprotein (LDL) levels. Optical coherence tomography images of the foveal avascular zone, retinal vascular density, choroidal flow, retinal thickness, CTh, and CVI were analyzed. Possible determining factors affecting CTh, CVI, and choriocapillaris flow were analyzed using nonparametric multivariate analysis. LDL (p < 0.001) positively correlated with CTh, whereas CVI (p = 0.007) was negatively correlated with CTh in diabetic patients with diabetes. We also identified a negative correlation between choriocapillaris flow and deep parafoveal retinal vessel density in patients with low-grade diabetic retinopathy (DR), which diminished in those with more advanced DR. Our study provides further information on the changes in choroidal structure and blood flow in patients with diabetes. Full article

To enhance the bioavailability and antihypertensive effect of the anti-depressant drug citalopram hydrobromide (CTH) we developed a sustained-release transdermal delivery system containing CTH. A transdermal diffusion meter was first used to determine the optimal formulation of the CTH transdermal drug delivery system (TDDS). Then, based on the determined formulation, a sustained-release patch was prepared; its physical characteristics, including quality, stickiness, and appearance, were evaluated, and its pharmacokinetics and irritation to the skin were evaluated by applying it to rabbits and rats. The optimal formulation of the CTH TDDS was 49.2% hydroxypropyl methyl cellulose K_100M, 32.8% polyvinylpyrrolidone K30, 16% oleic acid-azone, and 2% polyacrylic acid resin II. The system continuously released an effective dose of CTH for 24 h and significantly enhanced its bioavailability, with a higher area under the curve, good stability, and no skin irritation. The developed CTH TDDS possessed a sustained-release effect and good characteristics and pharmacokinetics; therefore, it has the potential for clinical application as an antidepressant. Full article