<p>Schematic representation of the Live Box 2 culture system used in this study. Reproduced with permission of IVTech.</p> Full article ">Figure 2
<p>Structural characterization of scaffold. eSEM analysis of Ctrl-sc (<b>A</b>,<b>B</b>) and FN-sc (<b>C</b>,<b>D</b>). There are different magnifications in each panel; from left to right: 200×; 800×.</p> Full article ">Figure 3
<p>μ-BCA results of Ctrl-sc and FN-sc read in absorbance at 560 nm. Error bars represent the standard deviation calculated on the mean of 6 scaffolds for each condition. Statistical analysis was performed using the Mann–Whitney test for comparison between the two groups, applying Bonferroni’s correction. Statistically significant differences are indicated as asterisks (*). *** = <span class="html-italic">p</span> < 0.001. N = 6.</p> Full article ">Figure 4
<p>Biocompatibility of Ctrl-sc (<span style="color:#FF6000">●</span>) and FN-sc (<span style="color:#077E97">■</span>) evaluated in terms of MG-63 adhesion and proliferation and compared with MG-63 (<span style="color:#FFA040">▲</span>) cultured in wells. Fluorescence intensity of Alamar Blue assay measured to evaluate cell adhesion (<b>A</b>) and cell proliferation within the scaffold (<b>B</b>). Error bars represent the standard deviation calculated on the mean of 3 scaffolds at each time point.</p> Full article ">Figure 5
<p>Cell adhesion (<b>A</b>) and viability (<b>B</b>) on Ctrl-sc (orange) and FN-sc (light blue) evaluated with the MTT assay; error bars represent the standard deviation calculated on the mean of 3 scaffolds at each time point. The statistical analysis was performed with the ANOVA test, applying Bonferroni’s correction. Statistically significant differences are indicated with asterisks (*). *** = <span class="html-italic">p</span> < 0.001. N = 3.</p> Full article ">Figure 6
<p>Top view of Ctrl-sc and FN-sc of MG-63 on day 1 (<b>A</b>–<b>D</b>) and 1 week (<b>E</b>–<b>H</b>) after seeding, at two different magnifications.</p> Full article ">Figure 7
<p>eSEM micrograph of cell (indicated by yellow arrows) seeded on Ctrl-sc (<b>A</b>–<b>C</b>), with a round shape morphology, and on FN-sc (<b>D</b>–<b>F</b>), with a well-spread morphology.</p> Full article ">Figure 8
<p>Proliferation of MG-63 on Ctrl-sc in static ((<b>A</b>), <span style="color:#FF6000">●</span>) and dynamic ((<b>B</b>), <span style="color:#00C000">●</span>) conditions, and FN-sc in static ((<b>A</b>), <span style="color:#077E97">■</span>) and dynamic ((<b>B</b>), <span style="color:#ff0c83">▲</span>) conditions, using a perfusion bioreactor. Error bars represent the standard deviation on the mean of 3 scaffolds.</p> Full article ">Figure 9
<p>eSEM micrographs of cells spread on FN-sc (<b>A</b>,<b>B</b>) and Ctrl-sc (<b>C</b>,<b>D</b>) after 14 days in a dynamic culture with a perfusion bioreactor.</p> Full article ">