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Agronomy
Special Issues
Recent Advancements in Nodulation Nitrogen Fixation and Molecular Regulation Mechanism...
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Recent Advancements in Nodulation Nitrogen Fixation and Molecular Regulation Mechanism in Soybean

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Crop Breeding and Genetics".

Deadline for manuscript submissions: 30 September 2024 | Viewed by 763

Special Issue Editor

Prof. Dr. Dawei Xin

E-Mail Website
Guest Editor
National Key Laboratory of Smart Farm Technology and System, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
Interests: symbiosis; soybean; type III effector; genetic population
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

As an important grain and oilseed crop, the nitrogen fixation capacity and nodule formation mechanism of soybean have been attracting much attention. This Special Issue aims to present the latest research progress in the field of soybean nodule formation, nitrogen fixation and molecular regulation, soybean genetics, genomics, germplasm mining and related biology progress in bio-nitrogen fixation and to provide a platform for scientists to communicate and share innovative ideas.

We especially welcome submissions on the latest research on soybean nodule development, molecular regulatory mechanisms in nitrogen fixation, as well as cutting-edge technologies and methods in related fields. We look forward to receiving original and prospective studies covering a wide range of aspects from basic science to applied fields. Possible topics include, but are not limited to, the diversity of symbiotic rhizobial microorganisms in soybean, signaling networks during rhizome formation, structure and function of nitrogen-fixing enzymes, and the application of gene editing technology in soybean nitrogen fixation research.

Prof. Dr. Dawei Xin
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • soybean
  • rhizobium
  • genome
  • genomic
  • germplasm
  • symbiosis
  • gene
  • field
  • function

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Published Papers (1 paper)

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Research

12 pages, 6497 KiB  
Article
Effects of GmERF5-Responsive Effector on Soybean Symbiotic Nodulation
by Candong Li, Yuxin Jin, Weinan Tang, Xuemei Li, Chunyan Liu, Jinhui Wang, Xiaoxia Wu, Qingshan Chen, Feishi Luan and Dawei Xin
Agronomy 2024, 14(6), 1239; https://doi.org/10.3390/agronomy14061239 - 7 Jun 2024
Viewed by 569
Abstract
The type III secretion system (T3SS) of Rhizobium plays a crucial role during the establishment of the soybean (Glycine max (L.) Merr.)-Rhizobium symbiosis system. Additionally, host-specific nodulation may also depend on the Nops (nodulation outer proteins) secreted by the T3SS of [...] Read more.
The type III secretion system (T3SS) of Rhizobium plays a crucial role during the establishment of the soybean (Glycine max (L.) Merr.)-Rhizobium symbiosis system. Additionally, host-specific nodulation may also depend on the Nops (nodulation outer proteins) secreted by the T3SS of Rhizobium. However, there is limited understanding of the response of soybean genes to Nops. In this study, a NopC mutant, HH103ΩNopC, was constructed from Sinorhizobium fredii HH103 and then utilized to assess the impact of the NopC on nodulation. An RNA-seq analysis revealed that the GmERF5 (Glycine max Ethylene Responsive Factor 5) gene was induced by NopC, which was confirmed by qRT-PCR. Furthermore, an overexpression of the GmERF5 hair-root system was constructed to investigate the effect of GmERF5 on nodulation and its interaction with NopC. Differences in nodule number and nodule dry weight in the overexpression of the GmERF5 hair-root system supported the conclusion that GmERF5 can regulate soybean nodule phenotype via NopC. These results elucidate the genetic mechanisms that regulate nodule phenotype in soybean. Full article
(This article belongs to the Special Issue Recent Advancements in Nodulation Nitrogen Fixation and Molecular Regulation Mechanism in Soybean)
►▼ Show Figures

Figure 1

Figure 1
<p>Amplification of <span class="html-italic">NopC</span> big fragment. Note: M: Trans 2K Plus DNA marker; 1, 2: <span class="html-italic">NopC</span> (1, 461 bp).</p> Full article ">Figure 2
<p><span class="html-italic">NopC</span> fragment insertion at mutation site. Note: M: Trans 2K Plus DNA marker; (<b>A</b>): lanes 1 and 2 for <span class="html-italic">NopC</span> upstream fragments; lanes 3 and 4 for <span class="html-italic">NopC</span> downstream fragments; (<b>B</b>): amplification of <span class="html-italic">NopC</span> mutant fragments.</p> Full article ">Figure 3
<p>Identification of nodules of different strains inoculated with SN14. RNA-seq analysis of <span class="html-italic">NopC</span> induced different expression genes. Note: (<b>A</b>) number of nodules; (<b>B</b>) dry weight of nodules; (<b>C</b>) number of large nodules (diameter ≥ 2 mm); (<b>D</b>) number of small nodules (diameter &lt; 2 mm) (where “**” represents <span class="html-italic">p</span> &lt; 0.01 and “*” represents <span class="html-italic">p</span> &lt; 0.05).</p> Full article ">Figure 4
<p>Venn diagram and heatmap of ERF family expression. (<b>a</b>) Identified DEGs induced by HH103 and <span class="html-italic">NopC</span> mutant; (<b>b</b>) five GmERF genes expression pattern.</p> Full article ">Figure 5
<p>Real-time quantification results of <span class="html-italic">GmERF5</span> gene.</p> Full article ">Figure 6
<p>Construction of <span class="html-italic">GmERF5</span> overexpression vector. Note: M: Trans 2K Plus DNA marker; (<b>A</b>) PCR identification of Fu28-<span class="html-italic">GmERF5</span> vector construct; (<b>B</b>) PCR identification of pSOY1-<span class="html-italic">GmERF5</span> vector construct; (<b>C</b>) PCR identification of <span class="html-italic">A. rhizogenes</span> K599 transformant.</p> Full article ">Figure 7
<p><span class="html-italic">GmERF5</span> gene rooting transformation method and identification results. Note: (<b>A</b>–<b>C</b>) soybean rooting transformation process; (<b>D</b>) UV lamp irradiation fluorescence GFP labeling detection.</p> Full article ">Figure 8
<p>qRT-PCR analysis of OE-<span class="html-italic">GmERF5</span> plants (where “**” represents <span class="html-italic">p</span> &lt; 0.01).</p> Full article ">Figure 9
<p>The qRT-PCR result of <span class="html-italic">GmERF5</span> gene overexpression plants.</p> Full article ">Figure 10
<p>Phenotypic identification of overexpression <span class="html-italic">GmERF5.</span> Note: (<b>A</b>) number of nodules; (<b>B</b>) dry weight of nodules; (<b>C</b>) number of large nodules (diameter ≥ 2 mm); (<b>D</b>) number of small nodules (diameter &lt; 2 mm) (where “**” represents <span class="html-italic">p</span> &lt; 0.01 and “*” represents <span class="html-italic">p</span> &lt; 0.05).</p> Full article ">
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