Identification of Two GDSL-Type Esterase/Lipase Genes Related to Tissue-Specific Lipolysis in Dendrobium catenatum by Multi-Omics Analysis
<p>Comprehensive identification of GELP family. (<b>a</b>) Phylogenetic analysis of GELPs in <span class="html-italic">D. catenatum</span>. A total of 52 GELPs were used to construct the unrooted maximum-likelihood phylogenies. (<b>b</b>) Domain organization of GELPs. Conservative blocks (Block I, II, III, and V) of GELPs were shown from up and down. Conservative amino acid residues Ser-Gly-Asn-His in blocks are marked by black triangles. (<b>c</b>) Expression patterns of GELP genes in different tissues. (<b>d</b>) Expression patterns of GELP genes in three flowering phases. S1, the flower buds were green in the early developmental stage; S2, the flowers had purple pigmentation in the columns and the lips; S3, the sepals and petals had turned yellow and red. (<b>e</b>) Lipidome analysis of four tissues in <span class="html-italic">D. catenatum</span>. Raw data is shown in <a href="#app1-life-12-01563" class="html-app">Table S1</a>. Lipid abbreviations is listed in <a href="#app1-life-12-01563" class="html-app">Table S2</a>. Color scales represented the values of log<sub>2</sub> in gene expression levels or lipid content.</p> "> Figure 2
<p>Identification and characterization of Dca016600. (<b>a</b>) Correlation analysis of lipidome and GELP expression levels. The dotted and solid lines, respectively, represent positive and negative correlations. The thickness of the line is determined by a Pearson correlation coefficient >0.9 or <−0.9, respectively. The dot sizes and colors represent the correlated number of lipids and genes. (<b>b</b>) Phylogenetic analysis of Dca016600 was intercepted to <a href="#app1-life-12-01563" class="html-app">Supplemental Figure S2</a>. Effects of pH (<b>c</b>), temperature (<b>d</b>), and the effect of substrate concentrations (<b>e</b>) for Dca016600 activity. Data represent the mean ± SD of three independent experiments. Km and Vmax values are determined using Lineweaver-Burk plot in <a href="#app1-life-12-01563" class="html-app">Figure S4</a>.</p> "> Figure 3
<p>Identification and expression analysis of Dca005399. (<b>a</b>) The relationship between changes in protein and mRNA abundances in FT vs. CK. (<b>b</b>) The relationship between changes in protein and mRNA abundances in FR vs. CK. The colored points indicate significant upregulation or downregulation of protein and mRNA levels. CK, control condition; FT, freezing treatment; FR, post-freezing recovery. (<b>c</b>) Phylogenetic analysis of Dca005399 was intercepted to <a href="#app1-life-12-01563" class="html-app">Supplemental Figure S2</a>. (<b>d</b>) Tissues expression analysis of <span class="html-italic">Dca005399</span>. Data represent the mean ± SD of three independent experiments. MS, mature seed; SGS, symbiotic germination seed; AGS, asymbiotic germination seed.</p> ">
Abstract
:1. Introduction
2. Results
2.1. Comprehensive Identification of GDSL Esterase/Lipase Protein (GELP) Family
2.2. Identification and Characterization of Dca016600
2.3. Identification and Expression Analysis of Dca005399
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Lipidomic Analysis
4.3. Phylogenetic Analysis
4.4. Dca016600 Activity Analysis
4.5. Real-Time Quantitative PCR
4.6. Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhan, X.; Qian, Y.; Mao, B. Identification of Two GDSL-Type Esterase/Lipase Genes Related to Tissue-Specific Lipolysis in Dendrobium catenatum by Multi-Omics Analysis. Life 2022, 12, 1563. https://doi.org/10.3390/life12101563
Zhan X, Qian Y, Mao B. Identification of Two GDSL-Type Esterase/Lipase Genes Related to Tissue-Specific Lipolysis in Dendrobium catenatum by Multi-Omics Analysis. Life. 2022; 12(10):1563. https://doi.org/10.3390/life12101563
Chicago/Turabian StyleZhan, Xinqiao, Yichun Qian, and Bizeng Mao. 2022. "Identification of Two GDSL-Type Esterase/Lipase Genes Related to Tissue-Specific Lipolysis in Dendrobium catenatum by Multi-Omics Analysis" Life 12, no. 10: 1563. https://doi.org/10.3390/life12101563