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Addgene

Microbiology Plasmids and Resources


Addgene’s repository contains plasmid tools for a range of microbial fields, including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with, or check out our additional microbiology resources.

The plasmids Addgene distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause. The standard format for providing individual plasmids is a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and distributions in accordance with export laws and biosafety regulations.

Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model organisms. See below for plasmid kits and other resources relevant to these organisms, or use our Search page to look for specific genes or applications that are not listed here.

Addgene Microbiology Resources

  • Plasmid Kits for Microbiology: Sets of plasmids for fluorescence, cloning and expression, and synthetic biology.
  • CRISPR Resources: Find essential background information on using CRISPR in your lab, specific CRISPR tools for genome editing in bacteria, and more.
  • Synthetic Biology: Browse our deposited tools and resources, or read our guide on assembly standards.

Browse Plasmids by Microbe System

Don’t see your field listed? Know of some great plasmid tools for the community that aren't in the repository? Help us widen our resources for the community by depositing your useful tools, or by suggesting plasmids for the collection.

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Plasmid Kits for Microbiology

Kits are sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item.

Fluorescent Proteins

Cloning and Expression

Synthetic Biology

  • BIOFAB plasmid set: E. coli gene expression - Endy Lab
  • CIDAR MoClo Parts Kit: Molecular cloning for E. coli - Densmore Lab
  • EcoFlex MoClo: Modular cloning for applications like recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab
  • MoClo-YTK: Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab
  • Yeast Prototrophy: Restoration of prototrophy in common S. cerevisiae lab strains - Ralser Lab

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Resources for Microbiology

General CRISPR Resources

Bacterial CRISPR Resources

Additional Addgene Resources

External Resources

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Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!