Pathology of pediatric tumors

Background High-risk human papillomaviruses (HPVs) are present and can cooperate with Epstein–Barr virus (EBV) to initiate and/or enhance the progression of several types of human carcinomas including cervical as well as head and neck; in parallel, it has been recently pointed out that these oncoviruses can be detected in human breast cancers. Thus, we herein explored the presence/co-presence of high-risk HPVs and EBV in breast cancer in Lebanese women. Methods A cohort of 102 breast cancer samples and 14 normal breast tissues were assessed for the presence of HPVs and EBV. Polymerase chain reaction (PCR) and immunohistochemistry (IHC) analysis in addition to tissue microarray (TMA) platform were used in this study. Results We found the presence of HPV in 66/102 (65%) of our samples, while EBV is present in 41/102 (40%) of the cohort. Additionally, our data showed that high-risk HPV types (52, 35, 58, 45, 16 and 51) are the most frequent in breast cancer in Lebanese women. Meanwhile...

Background High-risk human papillomaviruses (HPVs) are present and can cooperate with Epstein–Barr virus (EBV) to initiate and/or enhance the progression of several types of human carcinomas including cervical as well as head and neck; in parallel, it has been recently pointed out that these oncoviruses can be detected in human breast cancers. Thus, we herein explored the presence/co-presence of high-risk HPVs and EBV in breast cancer in Lebanese women. Methods A cohort of 102 breast cancer samples and 14 normal breast tissues were assessed for the presence of HPVs and EBV. Polymerase chain reaction (PCR) and immunohistochemistry (IHC) analysis in addition to tissue microarray (TMA) platform were used in this study. Results We found the presence of HPV in 66/102 (65%) of our samples, while EBV is present in 41/102 (40%) of the cohort. Additionally, our data showed that high-risk HPV types (52, 35, 58, 45, 16 and 51) are the most frequent in breast cancer in Lebanese women. Meanwhile...

Background: Chest tumours, in particular lung cancer, remain one of the most common causes of death worldwide. Using MD spiral CT, an increasing number of lung and mediastinal lesions is detected and histological diagnosis is often necessary to determine the most appropriate management of these lesions. Methods: Fine-needle aspiration biopsy (FNAB) and core-needle biopsy (CNB) currently is the predominant method for obtaining tissue specimens in patients with lung lesions. In many cases, treatment protocols are based on histological information. In 85 of all 97 patients included in ours study FNAB biopsy is performed and in 12 CNB, when technically feasible, or in cases where o ther techniques (such as bronchoscopy with lavage) are inconclusive. The 19-22G disposable needles were used. Results: In all 76 patients, aged 21-79 years with pulmonary lesions with dimensions of 2.0 cm or less FNB under CT control are performed. In 13 cases FNA under US control is performed due to the superficial localization of the lesions. Cytological and evaluation of FNAB samples were performed in all patients. Diagnostic sensitivity and accuracy are calculated. Assess the type of complications that occurred. FNAB and CNB, with the latter, demonstrated to have a slightly higher overall sensitivity, specificity and accuracy. Conclusion: Percutaneous FNAB and CNB are safe procedures even though a few complications are possible: pneumothorax, pulmonary haemorrhage is common, while air embolism and seeding are rare, but with severe consequences.