Microwave assisted biocatalytic transformation is a green process for chiral drug resolution. Differentimmobilized enzymes such as Novozym 435, Lipozyme TL IM, Lipozyme RM IM, Lipase Amano AS andLipase AYS amino were screened for the...
moreMicrowave assisted biocatalytic transformation is a green process for chiral drug resolution. Differentimmobilized enzymes such as Novozym 435, Lipozyme TL IM, Lipozyme RM IM, Lipase Amano AS andLipase AYS amino were screened for the kinetic resolution of RS-(±)-ketorolac under microwave irradia-tion. To study kinetics and mechanism, the effect of different parameters on the conversion, initial ratesand enantiomeric excess of substrate were studied. Under microwave synergism, an increase in initialrates up to 1.5-fold was observed. Novozym 435 effectively catalyzed the enantioselective esterificationof RS-(±)-ketorolac, showing excellent conversion (50%) and enantiomeric excess (>99%) in 3 h at 50◦Cand 300 rpm. Based on initial rate and progress curve data, the reaction was found to follow the PingPong bi–bi mechanism with inhibition by n-octanol.
