DMC

"Based on ethnographic interviews, this paper examines how Japanese hip-hop DJs distinguish themselves in the global marketplace in ways that reflect on Japan’s two self-images: its impenetrable uniqueness and its adeptness at assimilating other cultures (cf. Ivy, Iwabuchi). Following the autoexoticist strategies of Takemitsu and Akiyoshi, DJ Krush and Shing02 draw on Japanese uniqueness by integrating Japanese instruments (e.g. shakuhachi, shamisen, taiko), genres (biwa narrative), and aesthetics (ma, imperfection) into their works; Evis Beats takes a more parodic approach. At the DMC World Championships, Japanese DJs including DJ Kentarō have competed on the basis of eclecticism and originality in assimilating multiple sound sources. While countering the stereotype of the Japanese as imitators, this emphasis on originality may place some contestants too far from prevailing trends, putting them at a disadvantage. Both strategies imply that Japanese artists experience anxieties regarding their authenticity, necessitating strategies to differentiate themselves."

At this point of technological development the problem of illegal usage of electricity can be solved electronically without any human control .The implementation of this system will save large amount of electricity, and there by electricity will be available for more number of consumer then earlier in highly populated country such as INDIA. The proposed meter is set to carry a unique identification number such as the consumer's phone number which may be encrypted into the memory of the microcontroller. Electricity theft is being detected as the GSM module sends message to the distribution company. Revenue generated can be increased through the use of the proposed meter as unaccountability by utility workers and billing irregularities are eliminated. The results obtained from the simulation shows that immediately an illegal load is connected to the utility system either within the residential meter jurisdiction or otherwise stated, the GSM module alerts the utility company no matter how small the illegal load is so, The system prevents the illegal usage of electricity.

Currently protection codes are essential to memory cells using to maintain a good reliability, various error detection and error correction methods can be used but to avoid corruption data Error correction code (ECC) are mostly widely used and introduced a delay penalty in accessing the encoding and decoding process can be performed but limitation of ECC are low speed performed in memory .it can be contributed to the utilization of simple code such as single error correction (SEC) and Double error correction (DEC) and detection. Matrix Codes (MC) constructed on hamming codes has been proposed for memory protection. The main dispute is that they are double error correction codes and the error correction capabilities are not enhanced in all cases. Decimal Matrix Code (DMC) structure on matrix and divide symbol is proposed to improve memory reliability with lesser delay. In this thesis, 64-bits and 128-bits Decimal Matrix Code is offered to assure the memory reliability. Here to detect and correct up to 9 and 17 errors respectively. The offered DMC based on decimal algorithm to extended the number of detection and correction capability of error bits stored in memory. More ever structure area of DMC is reduced by reusing its Encoder this is called Encoder Reuse Techniques (ERT). ERT can be used to minimize area without interrupting DMC encoder and decoder. ERT Uses DMC Encoder itself to be a part of decoder. Hence the entire structure area of DMC can be minimized. Using Xilinx Design Suite 14.2 simulation and implementation analysis can be done. Next A Verilog Description has been adopted to embed the low power design.

Sumoylation is a post-translational modification system that covalently attaches the small ubiquitin-related modifier (SUMO) to target proteins. Ubc9 is required as the E2-type enzyme for SUMO-1 conjugation to targets. Here, we show that Ubc9 interacts with the meiosis-specific RecA homolog, Lim15/Dmc1 in the basidiomycete Coprinus cinereus (CcLim15), and mediates sumoylation of CcLim15 during meiosis. In vitro protein–protein interaction assays revealed that CcUbc9 interacts with CcLim15 and binds to the C-terminus (amino acids 105–347) of CcLim15, which includes the ATPase domain. Immunocytochemistry demonstrates that CcUbc9 and CcLim15 colocalize in the nuclei from the leptotene stage to the early pachytene stage during meiotic prophase I. Coimmunoprecipitation experiments indicate that CcUbc9 interacts with CcLim15 in vivo during meiotic prophase I. Furthermore, we show that CcLim15 is a target protein of sumoylation both in vivo and in vitro, and identify the C-terminus (amino acids 105–347) of CcLim15 as the site of sumoylation in vitro. These results suggest that sumoylation is a candidate modulator of meiotic recombination via interaction between Ubc9 and Lim15/Dmc1.

In eukaryotes, meiosis leads to genetically variable gametes through recombination between homologous chromosomes of maternal and paternal origin. Chromatin organization following meiotic recombination is critical to ensure the correct segregation of homologous chromosomes into gametes. However, the mechanism of chromatin organization after meiotic recombination is unknown. In this study we report that the meiosis-specific recombinase Lim15/Dmc1 interacts with the homologue of the largest subunit of chromatin assembly factor 1 (CAF-1) in the basidiomycete Coprinopsis cinerea (Coprinus cinereus). Using C. cinerea LIM15/DMC1 (CcLIM15) as the bait in a yeast two-hybrid screen, we have isolated the C. cinerea homologue of Cac1, the largest subunit of CAF-1 in Saccharomyces cerevisiae, and named it C. cinerea Cac1-like (CcCac1L). Two-hybrid assays confirmed that CcCac1L binds CcLim15 in vivo. β-Galactosidase assays revealed that the N-terminus of CcCac1L preferentially interacts with CcLim15. Co-immunoprecipitation experiments showed that these proteins also interact in the crude extract of meiotic cells. Furthermore, we demonstrate that, during meiosis, CcCac1L interacts with proliferating cell nuclear antigen (PCNA), a component of the DNA synthesis machinery recently reported as an interacting partner of Lim15/Dmc1. Taken together, these results suggest a novel role of the CAF-1–PCNA complex in meiotic events. We propose that the CAF-1–PCNA complex modulates chromatin assembly following meiotic recombination.

Sumoylation is a post-translational modification system that covalently attaches the small ubiquitin-related modifier (SUMO) to target proteins. Ubc9 is required as the E2-type enzyme for SUMO-1 conjugation to targets. Here, we show that Ubc9 interacts with the meiosis-specific RecA homolog, Lim15/Dmc1 in the basidiomycete Coprinus cinereus (CcLim15), and mediates sumoylation of CcLim15 during meiosis. In vitro protein–protein interaction assays revealed that CcUbc9 interacts with CcLim15 and binds to the C-terminus (amino acids 105–347) of CcLim15, which includes the ATPase domain. Immunocytochemistry demonstrates that CcUbc9 and CcLim15 colocalize in the nuclei from the leptotene stage to the early pachytene stage during meiotic prophase I. Coimmunoprecipitation experiments indicate that CcUbc9 interacts with CcLim15 in vivo during meiotic prophase I. Furthermore, we show that CcLim15 is a target protein of sumoylation both in vivo and in vitro, and identify the C-terminus (amino acids 105–347) of CcLim15 as the site of sumoylation in vitro. These results suggest that sumoylation is a candidate modulator of meiotic recombination via interaction between Ubc9 and Lim15/Dmc1.

In eukaryotes, meiosis leads to genetically variable gametes through recombination between homologous chromosomes of maternal and paternal origin. Chromatin organization following meiotic recombination is critical to ensure the correct segregation of homologous chromosomes into gametes. However, the mechanism of chromatin organization after meiotic recombination is unknown. In this study we report that the meiosis-specific recombinase Lim15/Dmc1 interacts with the homologue of the largest subunit of chromatin assembly factor 1 (CAF-1) in the basidiomycete Coprinopsis cinerea (Coprinus cinereus). Using C. cinerea LIM15/DMC1 (CcLIM15) as the bait in a yeast two-hybrid screen, we have isolated the C. cinerea homologue of Cac1, the largest subunit of CAF-1 in Saccharomyces cerevisiae, and named it C. cinerea Cac1-like (CcCac1L). Two-hybrid assays confirmed that CcCac1L binds CcLim15 in vivo. β-Galactosidase assays revealed that the N-terminus of CcCac1L preferentially interacts with CcLim15. Co-immunoprecipitation experiments showed that these proteins also interact in the crude extract of meiotic cells. Furthermore, we demonstrate that, during meiosis, CcCac1L interacts with proliferating cell nuclear antigen (PCNA), a component of the DNA synthesis machinery recently reported as an interacting partner of Lim15/Dmc1. Taken together, these results suggest a novel role of the CAF-1–PCNA complex in meiotic events. We propose that the CAF-1–PCNA complex modulates chromatin assembly following meiotic recombination.

In eukaryotes, meiosis leads to genetically variable gametes through recombination between homologous chromosomes of maternal and paternal origin. Chromatin organization following meiotic recombination is critical to ensure the correct segregation of homologous chromosomes into gametes. However, the mechanism of chromatin organization after meiotic recombination is unknown. In this study we report that the meiosis-specific recombinase Lim15/Dmc1 interacts with the homologue of the largest subunit of chromatin assembly factor 1 (CAF-1) in the basidiomycete Coprinopsis cinerea (Coprinus cinereus). Using C. cinerea LIM15/DMC1 (CcLIM15) as the bait in a yeast two-hybrid screen, we have isolated the C. cinerea homologue of Cac1, the largest subunit of CAF-1 in Saccharomyces cerevisiae, and named it C. cinerea Cac1-like (CcCac1L). Two-hybrid assays confirmed that CcCac1L binds CcLim15 in vivo. β-Galactosidase assays revealed that the N-terminus of CcCac1L preferentially interacts with CcLim15. Co-immunoprecipitation experiments showed that these proteins also interact in the crude extract of meiotic cells. Furthermore, we demonstrate that, during meiosis, CcCac1L interacts with proliferating cell nuclear antigen (PCNA), a component of the DNA synthesis machinery recently reported as an interacting partner of Lim15/Dmc1. Taken together, these results suggest a novel role of the CAF-1–PCNA complex in meiotic events. We propose that the CAF-1–PCNA complex modulates chromatin assembly following meiotic recombination.