AQUEOUS TULSI LEAF

In  the  present  study,  leaf  and  stem  of  Gloriosa  superba  L.  were
selected  to  evaluate  its  antioxidant  and  antibacterial  potential.  The
extraction was done by a cold percolation method using solvents like
hexane,  ethyl  acetate  and  methanol.  The  antioxidant  activity  was
evaluated using antioxidant assays like superoxide radical scavenging
assay,  Ferric  reducing  antioxidant  power  and  reducing  capacity
assessment.  Total  phenol  and  flavonoid  content  was  measured  by
Folin-  ciocalteu’s  reagent  and  aluminium  chloride  colorimetric
method respectively. The antibacterial activity was done by an agar
well  diffusion  method  against  four  Gram  positive  and  four  Gram
negative  bacteria.  The  methanol  extract  of  leaf  and  stem  showed
highest  FRAP  activity  and  highest  reducing  capacity assessment
respectively.  There  was  a  direct  correlation  between  total  phenolic
content  and  antioxidant  activity.  Gram  positive  bacteria  were  more
susceptible  than  Gram  negative  bacteria.  Methanolic extract  of  leaf
showed best antioxidant and antibacterial activity  and can be further
exploited as a natural source of antioxidant and antimicrobial.

Background: Consumption of high fat induces oxidative stress by disturbing the activity of a number of antioxidant enzymes in rat liver. The present study was designed to investigate the efficacy of the aqueous leaf extract of Tulsi (Ocimum sanctum) to provide protection against the High Fat Diet (HFD) induced oxidative injury in rat liver. Objective: The aim of the present study was to examine whether administration of aqueous extract of the leaves of Ocimum sanctum (Tulsi) do possess a protective effect against HFD-induced oxidative stress in rat liver. Materials and method: The dose and time dependent changes were studied in the liver of male Wistar rats following the oral feeding of the rats with HFD for a period of 5 days, 10 days and 15 days to determine the maximum oxidative damage in the hepatic tissue of rats without any mortality. Pre-treatment of rats with aqueous leaf extract of Tulsi (TLE) was also studied by oral administration of TLE with the increasing doses (50, 100, and 200 mg/kg BW) against HFD induced oxidative stress in rat liver to determine the minimum effective dose of Tulsi, which can provide protection to the liver damage and minimize the alterations in a concentration dependent manner. The alterations in the activity of some of the important different bio-markers of hepatic damage, biomarkers of oxidative stress, and activities of the antioxidant enzymes were studied. Histo-pathological and histo-chemical alterations were also studied through HE staining and Picrosirius red staining. Scanning electron microscopy was done to see the structural integrity of hepatic tissue surface. Result: The studies revealed that the extract protected the biomarkers of organ damage, oxidative stress, antioxidant enzymes, pro-oxidant enzymes and oxidative stress index from being altered in the rat hepatic tissue following treatment with HFD. Conclusion: It can be concluded from the current work that this aqueous leaf extract of Tulsi possess antioxidant properties and may be used as a potential antioxidant supplement to combat oxidative stress-induced tissue damages due to high fat diet consumption.

Solvent leaf extracts of Terminalia catappa were evaluated for
their cytotoxic and their radical scavenging potential. Brine shrimp
assay was used for exploring cytotoxic activity, all the extracts
namely hexane, DCM, chloroform, acetone and methanol were
tested at the dose level of 25, 50, 100 and 200microgram /ml. A
dose dependent response was obtained in all the extracts.
Chloroform extract showed 100percent activity at the highest dose
of 200μg/ml. Antioxidant activity was evaluated by one
qualitative(TLC based) and two quantitative radical scavenging
assays namely DPPH (2, 2- Diphenyl-1- picryl hydrazyl) assay
and ferric reducing antioxidant power (FRAP) assay. In both
assays acetone extract exhibited significant reduction of free
radicals. The IC50 value of acetone extract was 36.9μg/ml.
Ascorbic acid was used in these assays as reference compound.
FRAP value of acetone extract was found to be 6.73 much higher
than all the other extracts.

Objective: The present studies were aimed at evaluating whether an aqueous extract of Tulsi leaves is capable of ameliorating piroxicam–induced gastric injury in rats. Methods: Male Wister rats were used for the present studies. The aqueous Tulsi leaf extract (TLE) [25, 50, 100 and 200 mg/kg bw] was administered orally to the fasted rats (water ad libitum), one hour prior to the piroxicam treatment. Results: The aqueous leaf extract of Tulsi was found to protect the gastric mucosa from becoming ulcerated following piroxicam treatment, in a dose-dependent manner. Pre-treatment of rats with increasing doses of TLE protected the rise in the level of lipid peroxidation, reduced glutathione from being decreased as well as protected the activities of the antioxidant enzymes indicating it may have protected the gastric tissue from piroxicam-induced injury. The results of the biochemical analyses were supported by macroscopic and microscopic studies of gastric tissue. Conclusion: From the r...