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Protein Expression and Purification
REFOLD: An analytical database of protein refolding methods2006 •
Protein Science
Refolding and simultaneous purification by three-phase partitioning of recombinant proteins from inclusion bodies2008 •
Microbial cell factories
Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins2004 •
Recent advances in generating active proteins through refolding of bacterial inclusion body proteins are summarized in conjunction with a short overview on inclusion body isolation and solubilization procedures. In particular, the pros and cons of well-established robust refolding techniques such as direct dilution as well as less common ones such as diafiltration or chromatographic processes including size exclusion chromatography, matrix- or affinity-based techniques and hydrophobic interaction chromatography are discussed. Moreover, the effect of physical variables (temperature and pressure) as well as the presence of buffer additives on the refolding process is elucidated. In particular, the impact of protein stabilizing or destabilizing low- and high-molecular weight additives as well as micellar and liposomal systems on protein refolding is illustrated. Also, techniques mimicking the principles encountered during in vivo folding such as processes based on natural and artificia...
Journal of structural and functional genomics
Refolding strategies from inclusion bodies in a structural genomics project2004 •
The South-Paris Yeast Structural Genomics Project aims at systematically expressing, purifying and determining the structure of S. cerevisiae proteins with no detectable homology to proteins of known structure. We brought 250 yeast ORFs to expression in E. coli, but 37% of them form inclusion bodies. This important fraction of proteins that are well expressed but lost for structural studies prompted us to test methodologies to recover these proteins. Three different strategies were explored in parallel on a set of 20 proteins: (1) refolding from solubilized inclusion bodies using an original and fast 96-well plates screening test, (2) co-expression of the targets in E. coli with DnaK-DnaJ-GrpE and GroEL-GroES chaperones, and (3) use of the cell-free expression system. Most of the tested proteins (17/20) could be resolubilized at least by one approach, but the subsequent purification proved to be difficult for most of them.
Biotechnology Progress
Techno-economic evaluation of an inclusion body solubilization and recombinant protein refolding process2011 •
Expression of recombinant proteins in Escherichia coli is normally accompanied by the formation of inclusion bodies (IBs). To obtain the protein product in an active (native) soluble form, the IBs must be first solubilized, and thereafter, the soluble, often denatured and reduced protein must be refolded. Several technically feasible alternatives to conduct IBs solubilization and on-column refolding have been proposed
2016 •
Solubilized inclusion bodies (IBs) refolding process under low protein purity and high protein concentration conditions always re-aggregate targeted functional protein which is not applicable in nanobiotechnology and molecular biology applications. Enhanced green fluorescent protein (EGFP) IBs was used as the model protein in this study for investigating the effects of protein purity and concentration on the protein refolding process. Three different conditions of solubilized EGFP-IBs were self-refolded at 4°C: solubilized EGFP-IBs with cell debris; solubilized EGFP-IBs after detergent washing; and the purified solubilized EGFP-IBs by using preparative native urea polyacrylamide gel electrophoresis (PAGE). High protein concentration and low protein purity in first and second refolding conditions have resulted reaggregation of solubilized EGFP-IBs. Large molecular structure of self-refolded EGFP were formed and stuck at the top of stacking and resolving gels during the native PAGE pr...
BMC structural biology
REFOLDdb: a new and sustainable gateway to experimental protocols for protein refolding2017 •
More than 7000 papers related to "protein refolding" have been published to date, with approximately 300 reports each year during the last decade. Whilst some of these papers provide experimental protocols for protein refolding, a survey in the structural life science communities showed a necessity for a comprehensive database for refolding techniques. We therefore have developed a new resource - "REFOLDdb" that collects refolding techniques into a single, searchable repository to help researchers develop refolding protocols for proteins of interest. We based our resource on the existing REFOLD database, which has not been updated since 2009. We redesigned the data format to be more concise, allowing consistent representations among data entries compared with the original REFOLD database. The remodeled data architecture enhances the search efficiency and improves the sustainability of the database. After an exhaustive literature search we added experimental refol...
Quaternary International
Zooarchaeology in the Interconnected Mediterranean: Livestock Production and Biometric Change between the Metal Ages and Late Antiquity2023 •
Nora. Il foro romano. Storia di un'area urbana dall'età fenicia alla tarda antichità 1997-2006
La ceramica comune romana2009 •
In the World Yearbook of Education 2023: Racialization and Educational Inequality in Global Perspective. Edited By Janelle Scott and Monisha Bajaj, published November 7, 2022, by Routledge. ISBN 9781032148434. Chapter 11, pp 189-203.
Racialization and Resistance in South African Education2023 •
Slavic and East European Journal 53/3 (2009), 471–476
Forward into the past, or how to particularize new standard Ukrainian / уперед дозаду, або як "розхитати" новий стандарт української мови2009 •
MONÈRE RIVISTA DEI BENI CULTURALI E DELLE ISTITUZIONI POLITICHE
Monère, numero 3 anno2001 •
Arab World English Journal For Translation and Literary Studies
An Overview Concerning the Monologue and Stand-up Comedy2024 •
Ecology and Society
Using Coupled Simulation Models to Link Pastoral Decision Making and Ecosystem Services2011 •
Journal of Education, Health and Sport
Uric acid, neuroendocrine-immune complex and metabolism: relationships2023 •
Microbial Pathogenesis
Introduction of Francisella tularensis at skin sites induces resistance to infection and generation of protective immunity1992 •
Journal of Forecasting
Forecasting UK industrial production over the business cycle2001 •
SSRN Electronic Journal
From Airbnb to Solar: Toward a Transaction Cost Model of a Retail Electricity Distribution Platform2018 •
American Journal of Ophthalmology
Pathogenesis of Graves Ophthalmopathy: Implications for Prediction, Prevention, and Treatment2006 •