Papers by Panagiotis Berillis
Aquaculture reports, Jun 1, 2024
The aim of this study was to investigate the growth performance of gilthead sea bream (Sparus aur... more The aim of this study was to investigate the growth performance of gilthead sea bream (Sparus aurata) fed with a mixture of a single cell ingredient (SCI) (bacterial protein, yeast protein and algae) for organic aquaculture. Sea bream with an initial mean body weight of 6.87±0.07 g were randomly allocated to 250-litre tanks. Four isonitrogenous, isolipidic and isoenergetic diets were formulated by replacing organic fishmeal trimmings with 0% (control), 12% (SCI12), 15% (SCI15) and 18% (SCI18) of the SCI mixture. Each diet was distributed to the groups in triplicate. The fish were fed three times daily until satiation and the experimental period was 60 days. Survival rates and voluntary feed intake were similar in the fish with the different feed treatments (p > 0.05). Similar (p > 0.05) growth performance (final weight, SGR) and feed conversion ratio (FCR) were observed in fish fed all SCIbased diets, which was superior to the control diet treatment. Apparent protein digestibility was similar among treatments, but lipid digestibility was significantly reduced in the SCI15 and SCI18 groups compared to the SCI12 group. The specific activities of trypsin, total alkaline protease and lipase were increased in fish fed the SCI15 diet (p < 0.05). The protein content in the fillet was significantly increased and the lipid content was decreased in the SCI12 and SCI18 groups compared to the other groups. No adverse effects on liver and intestinal histology were observed in the different groups. The results suggest that organic fishmeal trimmings can be successfully replaced by innovative SCIs based on microbial protein, yeast meal and algae, and that this SCI mixture could be a candidate for organic aqua feed for gilthead sea bream.
Bookmarks Related papers MentionsView impact
functions and faster mortality. The findings of this study revealed that it is important to selec... more functions and faster mortality. The findings of this study revealed that it is important to select graphene materials with optimal structural and interfacial properties to achieve the highest insecticidal performance in potential development of a new generation of sustainable insecticides.
Bookmarks Related papers MentionsView impact
The aim of this study was to investigate the growth performance of gilthead sea bream (Sparus aur... more The aim of this study was to investigate the growth performance of gilthead sea bream (Sparus aurata) fed with a mixture of a single cell ingredient (SCI) (bacterial protein, yeast protein and algae) for organic aquaculture. Sea bream with an initial mean body weight of 6.87±0.07 g were randomly allocated to 250-litre tanks. Four isonitrogenous, isolipidic and isoenergetic diets were formulated by replacing organic fishmeal trimmings with 0% (control), 12% (SCI12), 15% (SCI15) and 18% (SCI18) of the SCI mixture. Each diet was distributed to the groups in triplicate. The fish were fed three times daily until satiation and the experimental period was 60 days. Survival rates and voluntary feed intake were similar in the fish with the different feed treatments (p > 0.05). Similar (p >0.05) growth performance (final weight, SGR) and feed conversion ratio (FCR) were observed in fish fed all SCIbased diets, which was superior to the control diet treatment. Apparent protein digestibility was similar among treatments, but lipid digestibility was significantly reduced in the SCI15 and SCI18 groups compared to the SCI12 group. The specific activities of trypsin, total alkaline protease and lipase were increased in fish fed the SCI15 diet (p < 0.05). The protein content in the fillet was significantly increased and the lipid content was decreased in the SCI12 and SCI18 groups compared to the other groups. No adverse effects on liver and intestinal histology were observed in the different groups. The results suggest that organic fishmeal trimmings can be successfully replaced by innovative SCIs based on microbial protein, yeast meal and algae, and that this SCI mixture could be a candidate for organic aqua feed for gilthead sea bream.
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
The aim of this study was to investigate the growth performance of gilthead sea bream (Sparus aur... more The aim of this study was to investigate the growth performance of gilthead sea bream (Sparus aurata) fed with a mixture of a single cell ingredient (SCI) (bacterial protein, yeast protein and algae) for organic aquaculture. Sea bream with an initial mean body weight of 6.87±0.07 g were randomly allocated to 250-litre tanks. Four isonitrogenous, isolipidic and isoenergetic diets were formulated by replacing organic fishmeal trimmings with 0% (control), 12% (SCI12), 15% (SCI15) and 18% (SCI18) of the SCI mixture. Each diet was distributed to the groups in triplicate. The fish were fed three times daily until satiation and the experimental period was 60 days. Survival rates and voluntary feed intake were similar in the fish with the different feed treatments (p > 0.05). Similar (p > 0.05) growth performance (final weight, SGR) and feed conversion ratio (FCR) were observed in fish fed all SCIbased diets, which was superior to the control diet treatment. Apparent protein digestibility was similar among treatments, but lipid digestibility was significantly reduced in the SCI15 and SCI18 groups compared to the SCI12 group. The specific activities of trypsin, total alkaline protease and lipase were increased in fish fed the SCI15 diet (p < 0.05). The protein content in the fillet was significantly increased and the lipid content was decreased in the SCI12 and SCI18 groups compared to the other groups. No adverse effects on liver and intestinal histology were observed in the different groups. The results suggest that organic fishmeal trimmings can be successfully replaced by innovative SCIs based on microbial protein, yeast meal and algae, and that this SCI mixture could be a candidate for organic aqua feed for gilthead sea bream.
Bookmarks Related papers MentionsView impact
Journal of Fisheriessciences.com, 2015
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Frontiers in Physiology
Single-cell ingredients (SCI) are considered promising nutrient sources which are produced using ... more Single-cell ingredients (SCI) are considered promising nutrient sources which are produced using environmentally friendly biotechnological processes. The aim of the current study was to evaluate the replacement of fishmeal, plant protein sources, and fish oil with SCI in organic feeds for European sea bass (Dicentrarchus labrax). Bacterial protein, yeast protein, and microalgae were used to replace fishmeal trimmings, soya bean meal, and fish oil from trimmings. Triplicate groups (30 fish per replicate) of European sea bass (14.4 ± 2.4 g) were fed the experimental diets for 71 days. The results showed that the incorporation of SCI at all levels of inclusion significantly enhanced nutrient digestibility. Additionally, growth performance parameters were not affected by SCI inclusion, exhibiting similar or improved values. Moreover, a tendency for improved anterior and posterior gut structure was observed and a significant increase of lysozyme activity at the two highest inclusion leve...
Bookmarks Related papers MentionsView impact
Aquaculture
Bookmarks Related papers MentionsView impact
Applied Sciences
The possibility of simultaneous production of halophyte and euryhaline fish creates huge interest... more The possibility of simultaneous production of halophyte and euryhaline fish creates huge interest in both commercial aquaponics systems and in research. The aim of the present study was to investigate the effect of three different salinities (8, 14, and 20 ppt) on the growth performance and survival rate of sea bream (Sparus aurata) and rock samphire (Crithmum maritimum) in an experimental brackish aquaponic system. Furthermore, induction of heat shock proteins (Hsps) and phosphorylation of mitogen-activated protein kinases (MAPKs) were assessed through the sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot analysis. A total number of 234 sea bream individuals were divided into nine autonomous aquaponic systems. The experiment lasted 45 days. In total, 54 individuals of rock samphire were used and were distributed into groups of six individuals per hydroponic tank using the raft method. Water quality showed stable fluctuation throughout the experimen...
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Bookmarks Related papers MentionsView impact
Fishes
The aim of this study is to investigate the effect of 10 and 20% replacement of fishmeal (FM diet... more The aim of this study is to investigate the effect of 10 and 20% replacement of fishmeal (FM dietary group) with the superworm Zophobas morio larvae meal (ZM10 and ZM20 dietary groups) on the growth performance of sea bass (Dicentrarchus labrax) and lettuce plants (Lactuca sativa) reared in aquaponics. Ninety juvenile sea bass with an average body weight of 21.55 g/individual were placed in aquaponic fish tanks, together with 24 lettuce plants with an average initial height of 8.90 cm/plant and an average number of leaves of 5.75/plant over a 45-day trial period. At the end of that period, all feeding groups exhibited high survival. In fact, ΖΜ10 and ZM20 groups showed similar fish weight gain and specific growth rate (SGR) (p > 0.05), but significantly lower SGR (p < 0.05) than the FM group. Nevertheless, final fish body weight and length were similar (p > 0.05) for all feeding groups. No plant mortality was observed during the 45-day study period. All three aquaponic syst...
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Applied Sciences, 2021
Herein we investigated the cellular responses of the endemic fish species Alburnus vistonicus Fre... more Herein we investigated the cellular responses of the endemic fish species Alburnus vistonicus Freyhof & Kottelat, 2007, under the variation of several physico-chemical parameters including temperature (°C), salinity (psu), dissolved oxygen (mg/L), pH and conductivity (μS/cm), which were measured in situ. Monthly fish samplings (October 2014–September 2015) were conducted in Vistonis Lake in northern Greece, a peculiar ecosystem with brackish waters in its southern part and high salinity fluctuations in its northern part. Fish gills and liver responses to the changes of the physico-chemical parameters were tested biochemically and histologically. Heat shock protein levels appeared to be correlated with salinity fluctuations, indicating the adaptation of A. vistonicus to the particular environment. The latter is also enhanced by increased Na+-K+ ATPase levels, in response to salinity increase during summer. The highest mitogen activated protein kinases phosphorylation levels were obse...
Bookmarks Related papers MentionsView impact
Journal of FisheriesSciences.com, 2018
Bookmarks Related papers MentionsView impact
International journal of food microbiology, Jan 21, 2018
The progressive ability of a six-strains L. monocytogenes cocktail to form biofilm on stainless s... more The progressive ability of a six-strains L. monocytogenes cocktail to form biofilm on stainless steel (SS), under fish-processing simulated conditions, was investigated, together with the biocide tolerance of the developed sessile communities. To do this, the pathogenic bacteria were left to form biofilms on SS coupons incubated at 15°C, for up to 240h, in periodically renewable model fish juice substrate, prepared by aquatic extraction of sea bream flesh, under both mono-species and mixed-culture conditions. In the latter case, L. monocytogenes cells were left to produce biofilms together with either a five-strains cocktail of four Pseudomonas species (fragi, savastanoi, putida and fluorescens), or whole fish indigenous microflora. The biofilm populations of L. monocytogenes, Pseudomonas spp., Enterobacteriaceae, HS producing and aerobic plate count (APC) bacteria, both before and after disinfection, were enumerated by selective agar plating, following their removal from surfaces t...
Bookmarks Related papers MentionsView impact
Journal of FisheriesSciences.com, 2017
Bookmarks Related papers MentionsView impact
Marine drugs, Jan 29, 2017
In search of alternative and safer sources of collagen for biomedical applications, the marine de... more In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges Axinella cannabina and Suberites carnosus, collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spongin-like collagen content. The isolated collagenous materials were morphologically, physicochemically, and biophysically characterized. Using scanning electron microscopy and transmission electron microscopy the fibrous morphology of the isolated collagens was confirmed, whereas the amino acid analysis, in conjunction with infrared spectroscopy studies, verified the characteristic for the collagen amino acid profile and its secondary structure. Furthermore, the isoelectric point and thermal behavior were determined by titration and differential scanning calorimetry, in combination with circular dichroism spectroscopic studies, respectively.
Bookmarks Related papers MentionsView impact
Journal of Stored Products Research, 2016
Abstract Laboratory studies were conducted in order to evaluate the insecticidal potential of thr... more Abstract Laboratory studies were conducted in order to evaluate the insecticidal potential of three commercially available zeolite formulations against adults of Sitophilus oryzae , Tribolium confusum and Oryzaephilus surinamensis in wheat. For each zeolite formulation, three particle size levels were tested, i.e. 0–50, 0–150 and 0–500 μm. Zeolites were applied at three dose rates, 250, 500 and 1000 ppm, and insect mortality was assessed after 2, 7, 14 and 21 d of exposure. After the final mortality count, dead and alive insects were removed and offspring numbers were determined following an additional period of 65 d. In another series of laboratory bioassays, the effect of zeolite application on the test weight of wheat, maize and barley, as well as the adherence of zeolite particles to wheat, maize, barley and rice kernels was also measured. Oryzaephilus surinamensis was the most susceptible species to zeolite application, regardless of the zeolite formulation, dose and particle size level tested, whereas T . confusum was the most tolerant. No significant differences in efficacy were recorded among the three tested zeolite formulations. At the same time, particle size did not affect zeolite efficacy, at least for the particle size levels tested. All zeolites caused a significant reduction on the test weight of the treated grains. Moreover, zeolite particles showed different adherence among wheat, maize, barley and rice kernels. The results of the present study indicate that zeolites can be used with success as grain protectants, but there is a considerable effect on some physical properties of the grains. This information aims to encourage further evaluation of zeolites as grain protectants.
Bookmarks Related papers MentionsView impact
Uploads
Papers by Panagiotis Berillis
Existing evidence based on the prevailing microorganisms and cyanotoxin levels in the lake in 2010 suggested that the lake
could have a negative effect on its biota. By investigating whether Carassius gibelio individuals bear brain and liver
histopathological findings, this study complements the data now available from the first year of L. Karla’s reconstruction.
Based on the 16S rRNA gene diversity, the potentially toxic cyanobacteria Anabaenopsis sp. and Planktothrix were shown to
be present in the lake’s water in May 2011. C. gibelio specimens were collected a month later. Histopathological alterations in
the liver of the individuals, namely picnotic nuclei, loss of structure and hemorrhagic regions, were detected by microscopic
examination. Picnotic nuclei, loss of the liver structure and hemorrhagic regions were detected. These alterations may be
associated with the occurrence of cyanobacterial toxins possibly produced by the potentially toxic cyanobacterial species
found in the water column. No alterations were found in the brain, probably due to the existence of the blood-brain barrier.
Trends in Fisheries and Aquatic Animal Health covers some basic and applied topics in fishery management and fish health with a focus on European regions. The textbook is a combination of reviews and research articles. Topics covered in the book include challenges in fishery management, environmental impacts on fisheries, fish health (pharmacology, histopathology, stress response), telemetry techniques in fisheries research, and specific case studies of regional marine species in localized fisheries. This textbook is a useful resource for graduates and professionals involved in advanced training courses for aquaculture and fishery management.