Tomas Remenyi
University of Tasmania, Institute for Marine and Antarctic Studies, Graduate Student
A fast and sensitive reversed-phase high-performance liquid-chromatographic method for determination of aluminium in aqueous samples has been developed. The fluorescent aluminiumlumogallion complex (lex 505 nm, lem 574 nm), was formed... more
A fast and sensitive reversed-phase high-performance liquid-chromatographic method for determination of aluminium in aqueous samples has been developed. The fluorescent aluminiumlumogallion complex (lex 505 nm, lem 574 nm), was formed with a pre-column reagent and then separated on a Chromolith® RP-18e Guard column using a two-tiered, stepped gradient program, with matrix elimination (5/95 (v/v) methanol/water), followed by elution (90/10 (v/v) methanol/water). This method uses MES buffer (2-(N-morpholino)ethanesulfonic acid), which compared to other buffers is more easily purified, does not complex with aluminium, and can be used in lower concentrations. This method was applied to analysis of: deionised water for column comparison; seawater for matrix
interference effects; and tea-infusion for calibration and recovery studies.
interference effects; and tea-infusion for calibration and recovery studies.
