Luis M Botana
Fogarty Fellow at Johns Hopkins Asthma and Allergy Center, Baltimore, MD, US. 1990-1991
Director or EU-RL for marine toxins. 2004-2009
Director of Dept Pharmacology of USC. 2003-2011
Full Professor since 1998.
Phone: +34982822233
Address: Departamento de Farmacología
Facultad de Veterinaria
Campus de Lugo-USC 27002
Lugo
Director or EU-RL for marine toxins. 2004-2009
Director of Dept Pharmacology of USC. 2003-2011
Full Professor since 1998.
Phone: +34982822233
Address: Departamento de Farmacología
Facultad de Veterinaria
Campus de Lugo-USC 27002
Lugo
less
InterestsView All (8)
Uploads
Papers by Luis M Botana
from Sphaerococcus coronopifolius. The cytotoxicity was evaluated on malignant cell lines (A549, CACO-2, HCT-15, MCF-7, NCI-H226, PC-3, SH-SY5Y, and SK-MEL-28) using the MTT and LDH assays. The ability of compounds to stimulate the production of hydrogen peroxide (H2O2) and
to induce mitochondrial dysfunction, the externalization of phosphatidylserine, Caspase-9 activity, and changes in nuclear morphology was also studied on MCF-7 cells. The ability to induce DNA
damage was also studied on L929 fibroblasts. The proteasome inhibitory activity was estimated through molecular docking studies. The compounds exhibited IC50 values between 15.35 and 53.34
µM. 12R-hydroxy-bromosphaerol and 12S-hydroxy-bromosphaerol increased the H2O2 levels on MCF-7 cells, and bromosphaerol induced DNA damage on fibroblasts. All compounds promoted a depolarization of mitochondrial membrane potential, Caspase-9 activity, and nuclear condensation and fragmentation. The compounds have been shown to interact with the chymotrypsin-like catalytic site through molecular docking studies; however, only 12S-hydroxy-bromosphaerol evidenced interaction with ALA20 and SER169, key residues of the proteasome catalytic mechanism. Further studies should be outlined to deeply characterize and understand the potential of those bromoditerpenes for
anticancer therapeutics.
account its presence in a healthy diet.
risk factors. Therefore, this study aimed to determine the association between cyclophilin A, B, C, and D and cardiovascular risk factors in coronary artery disease.
Serum levels of cyclophilins were measured in 167 subjects (subdivided according to cardiovascular risk factors presence). This study reveals that cyclophilin A and C are elevated in patients regardless of the risk factors presence. Moreover, cyclophilin B is elevated in male patients with hypertension, type 2 diabetes, or high glucose levels. In addition, cyclophilins A, B, and C were signi cantly correlated with cardiovascular risk factors, but only cyclophilin B was associated with type 2 diabetes. The multivariate analysis strengthens the predictive value for coronary artery disease presence of cyclophilin A (>8.2 ng/mL) and cyclophilin C (>17.5 pg/mL) along with the cardiovascular risk factors tobacco, hypertension, dyslipidemia, and high glucose and cholesterol levels. Moreover, the risk of coronary artery disease is increased in presence of cyclophilin B levels above 63.26 pg/mL and with hypertension or dyslipidemia in male patients. Consequently, cyclophilins A and C serum levels are reinforced as useful coronary artery disease biomarkers, meanwhile, cyclophilin B is a valuable biomarker in the male population when patients are also suffering from hypertension or dyslipidemia.
the sensitization with O/V due to its expression of sodium channels. In the case of sensitizing the cells with O/V, it was possible to detect the presence of the ciguatoxin by the classical cytotoxicity MTT method at concentrations as low as 0.0001 nM CTX3C, providing an alternative cell line for the detection of compounds that act on the sodium channels.
the food chain by accumulating in seafood. They are responsible for Diarrhetic Shellfish Poisoning (DSP) events
in humans over the world and therefore are also jointly named as Diarrhetic Shellfish Toxins (DSTs). The main
objective of this study was to evaluate symptoms, toxicity, absorption, distribution, and elimination of OA,
Dinophysistoxin-1 (DTX1), and Dinophysistoxin-2 (DTX2) at the sublethal dose of 90 μg toxin/kg bw administered
through voluntary feeding to mice. The toxin comparison highlighted that OA and DTX1 induced more
severe and specific symptoms such as diarrhea. After oral ingestion toxins were distributed through the entire
organism being detected in liver, kidney, stomach, small and large intestine. Predominant excretion of the toxins
was observed in feces, with OA exhibiting fast elimination, while DTX2 was showing prolonged excretion. The passage and accumulation of toxins in gastrointestinal organs instigated macroscopic damage in the stomach,
small and large intestine that could persist up to 120 h. These findings highlight the importance of pharmacokinetic
of sublethal doses of DSTs administered by voluntary feeding in their toxicity and their implication for
public health.
from Sphaerococcus coronopifolius. The cytotoxicity was evaluated on malignant cell lines (A549, CACO-2, HCT-15, MCF-7, NCI-H226, PC-3, SH-SY5Y, and SK-MEL-28) using the MTT and LDH assays. The ability of compounds to stimulate the production of hydrogen peroxide (H2O2) and
to induce mitochondrial dysfunction, the externalization of phosphatidylserine, Caspase-9 activity, and changes in nuclear morphology was also studied on MCF-7 cells. The ability to induce DNA
damage was also studied on L929 fibroblasts. The proteasome inhibitory activity was estimated through molecular docking studies. The compounds exhibited IC50 values between 15.35 and 53.34
µM. 12R-hydroxy-bromosphaerol and 12S-hydroxy-bromosphaerol increased the H2O2 levels on MCF-7 cells, and bromosphaerol induced DNA damage on fibroblasts. All compounds promoted a depolarization of mitochondrial membrane potential, Caspase-9 activity, and nuclear condensation and fragmentation. The compounds have been shown to interact with the chymotrypsin-like catalytic site through molecular docking studies; however, only 12S-hydroxy-bromosphaerol evidenced interaction with ALA20 and SER169, key residues of the proteasome catalytic mechanism. Further studies should be outlined to deeply characterize and understand the potential of those bromoditerpenes for
anticancer therapeutics.
account its presence in a healthy diet.
risk factors. Therefore, this study aimed to determine the association between cyclophilin A, B, C, and D and cardiovascular risk factors in coronary artery disease.
Serum levels of cyclophilins were measured in 167 subjects (subdivided according to cardiovascular risk factors presence). This study reveals that cyclophilin A and C are elevated in patients regardless of the risk factors presence. Moreover, cyclophilin B is elevated in male patients with hypertension, type 2 diabetes, or high glucose levels. In addition, cyclophilins A, B, and C were signi cantly correlated with cardiovascular risk factors, but only cyclophilin B was associated with type 2 diabetes. The multivariate analysis strengthens the predictive value for coronary artery disease presence of cyclophilin A (>8.2 ng/mL) and cyclophilin C (>17.5 pg/mL) along with the cardiovascular risk factors tobacco, hypertension, dyslipidemia, and high glucose and cholesterol levels. Moreover, the risk of coronary artery disease is increased in presence of cyclophilin B levels above 63.26 pg/mL and with hypertension or dyslipidemia in male patients. Consequently, cyclophilins A and C serum levels are reinforced as useful coronary artery disease biomarkers, meanwhile, cyclophilin B is a valuable biomarker in the male population when patients are also suffering from hypertension or dyslipidemia.
the sensitization with O/V due to its expression of sodium channels. In the case of sensitizing the cells with O/V, it was possible to detect the presence of the ciguatoxin by the classical cytotoxicity MTT method at concentrations as low as 0.0001 nM CTX3C, providing an alternative cell line for the detection of compounds that act on the sodium channels.
the food chain by accumulating in seafood. They are responsible for Diarrhetic Shellfish Poisoning (DSP) events
in humans over the world and therefore are also jointly named as Diarrhetic Shellfish Toxins (DSTs). The main
objective of this study was to evaluate symptoms, toxicity, absorption, distribution, and elimination of OA,
Dinophysistoxin-1 (DTX1), and Dinophysistoxin-2 (DTX2) at the sublethal dose of 90 μg toxin/kg bw administered
through voluntary feeding to mice. The toxin comparison highlighted that OA and DTX1 induced more
severe and specific symptoms such as diarrhea. After oral ingestion toxins were distributed through the entire
organism being detected in liver, kidney, stomach, small and large intestine. Predominant excretion of the toxins
was observed in feces, with OA exhibiting fast elimination, while DTX2 was showing prolonged excretion. The passage and accumulation of toxins in gastrointestinal organs instigated macroscopic damage in the stomach,
small and large intestine that could persist up to 120 h. These findings highlight the importance of pharmacokinetic
of sublethal doses of DSTs administered by voluntary feeding in their toxicity and their implication for
public health.