Papers by Santos Villanueva
European Neuropsychopharmacology, 2009
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The use of nuclear methods can enhance several features of DNA vaccines in protecting livestock a... more The use of nuclear methods can enhance several features of DNA vaccines in protecting livestock against pathogens. While DNA vaccines already have several advantages over their traditional predecessors (e.g. cheap production, stability over a wide range of temperature, amenability to genetic manipulation, and no risk of reversion to pathogenicity), conventional gene delivery systems make immunization of livestock and aquaculture populations tedious. For this reason, we are developing radiation-synthesized intelligent delivery systems for DNA vaccines. We encapsulated a reporter construct pCMV•SPORT-β-gal in radiation-synthesized κ-carrageenan-polyvinylpyr-rolidone microspheres IP20 (for stomach release) and IP18 (for intestinal release). The DNA-loaded polymers were orally administered to Oreochromis niloticus (black Nile tilapia), and whole organs were stained with X-gal to observe β-galactosidase activity. Intense staining was observed in the stomach regions with IP20, while minimal staining was observed with IP18. The gills, in contrast, did not express β-galactosidase activity. Our results show evidence of the successful gene delivery capabilities of radiation-synthesized microspheres.
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Process Biochemistry, 2004
... The cells were grown in Fernbach flasks of 2.5 l containing 2 l of BG-11 medium [13] at 28 °C... more ... The cells were grown in Fernbach flasks of 2.5 l containing 2 l of BG-11 medium [13] at 28 °C for 8 days, the cultures were supplied with 1 vvm air flow rate and continuous illumination (180 μE/(m 2 s)) provided by fluorescent daylight lamps. ...
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Anales De Pediatria, 2005
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Schizophrenia Research, 2010
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Journal of Nuclear Cardiology, 2005
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Schizophrenia Research, 2008
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Anales De Medicina Interna, 2005
... An Med Interna (Madrid) 2004; 21: 514-515. 2. Pritt B, Harmon M, Schwartz M, Cooper K. A tale... more ... An Med Interna (Madrid) 2004; 21: 514-515. 2. Pritt B, Harmon M, Schwartz M, Cooper K. A tale of three aspirations: foreign bodies in the airway. J Clin Pathol 2003; 56: 791-4. ... Crit Care Clin 2000; 16: 83-100. © 2011 Arán Ediciones, SL Castelló, 128, 1º 28006-MADRID Telf. ...
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Schizophrenia Research, 2008
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Journal of The American Academy of Dermatology, 2004
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Nuclear Instruments & Methods in Physics Research Section A-accelerators Spectrometers Detectors and Associated Equipment, 2007
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Respirology, 2007
Background and objectives: Pulmonary nocardiosis (PN) is an infrequent but severe infection caus... more Background and objectives: Pulmonary nocardiosis (PN) is an infrequent but severe infection caused by Nocardia spp., which can behave either as opportunistic or primary pathogens. The present study identifies the risk factors for PN, clinical symptoms and radiographic features and the factors that affect its prognosis.Methods: An observational study of all the patients diagnosed with PN over a 13-year period at the authors’ institution.Results: Thirty-one adult patients were identified with PN, 11 of whom had disseminated nocardiosis. The predisposing conditions were COPD (23%), transplantation (29%), HIV infection (19%), alcoholism (6.5%) and treatment with steroids (64.5%). Respiratory tract sampling using non-invasive techniques had a diagnostic yield of 77%, while specimens from invasive methods had a yield of 47%. Mean time to diagnosis was 42 days. Dissemination to the central nervous system was related to alcoholism. The mortality rates were 41% for PN and 64% for disseminated nocardiosis; when Nocardia disseminated to the central nervous system, the mortality was 100%.Conclusion: Specific risk factors were found in 94% of patients, with the most common being corticosteroid treatment and immunosuppressive therapy. The time to reach diagnosis and to prescribe specific treatment was considerable and mandatory assessment for nocardia in high-risk patients is required. The mortality rate of PN is high and early diagnosis and treatment are needed. Medications other than co-trimoxazole may be required.
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Papers by Santos Villanueva